Flavisolibacter ginsengisoli DSM 18119
Average proteome isoelectric point is 6.94
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4178 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1M5CRL0|A0A1M5CRL0_9BACT ATP synthase subunit beta OS=Flavisolibacter ginsengisoli DSM 18119 OX=1121884 GN=atpD PE=3 SV=1
MM1 pKa = 8.11 DD2 pKa = 4.95 SLHH5 pKa = 6.45 NVIDD9 pKa = 4.03 PEE11 pKa = 5.08 IGLNIVDD18 pKa = 5.22 LGLIYY23 pKa = 10.54 QVDD26 pKa = 4.26 FDD28 pKa = 6.55 DD29 pKa = 4.19 DD30 pKa = 4.05 TKK32 pKa = 10.84 TCFVSMTLTTQFCPMGEE49 pKa = 4.35 SIRR52 pKa = 11.84 DD53 pKa = 3.58 NVVNALEE60 pKa = 4.11 QAFPGYY66 pKa = 10.35 AINVDD71 pKa = 4.25 LVFDD75 pKa = 4.9 PPWSTEE81 pKa = 3.83 KK82 pKa = 10.82 VSEE85 pKa = 4.44 QGLKK89 pKa = 10.04 YY90 pKa = 10.95 LNGEE94 pKa = 4.07
Molecular weight: 10.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.745
IPC2_protein 3.77
IPC_protein 3.719
Toseland 3.503
ProMoST 3.859
Dawson 3.719
Bjellqvist 3.948
Wikipedia 3.681
Rodwell 3.554
Grimsley 3.427
Solomon 3.694
Lehninger 3.656
Nozaki 3.846
DTASelect 4.075
Thurlkill 3.579
EMBOSS 3.681
Sillero 3.846
Patrickios 1.875
IPC_peptide 3.694
IPC2_peptide 3.808
IPC2.peptide.svr19 3.788
Protein with the highest isoelectric point:
>tr|A0A1M5BYY4|A0A1M5BYY4_9BACT Hemerythrin HHE cation binding domain-containing protein OS=Flavisolibacter ginsengisoli DSM 18119 OX=1121884 GN=SAMN02745131_02741 PE=4 SV=1
MM1 pKa = 6.69 VAKK4 pKa = 8.98 VHH6 pKa = 6.23 PLGLSRR12 pKa = 11.84 RR13 pKa = 11.84 MKK15 pKa = 10.41 GSFRR19 pKa = 11.84 GTRR22 pKa = 11.84 NLWIVDD28 pKa = 3.35 GVARR32 pKa = 11.84 VLWIKK37 pKa = 10.15 VQAFAIKK44 pKa = 10.61 NCVLWNDD51 pKa = 3.55 CLRR54 pKa = 11.84 WFTTYY59 pKa = 10.48 YY60 pKa = 10.42 FCLPSASHH68 pKa = 6.82 FLLMPKK74 pKa = 10.13 RR75 pKa = 11.84 KK76 pKa = 9.56
Molecular weight: 8.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.331
IPC2_protein 9.867
IPC_protein 10.643
Toseland 10.935
ProMoST 10.687
Dawson 11.008
Bjellqvist 10.716
Wikipedia 11.213
Rodwell 11.316
Grimsley 11.038
Solomon 11.155
Lehninger 11.111
Nozaki 10.935
DTASelect 10.701
Thurlkill 10.921
EMBOSS 11.345
Sillero 10.95
Patrickios 11.082
IPC_peptide 11.155
IPC2_peptide 9.911
IPC2.peptide.svr19 8.433
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4178
0
4178
1362833
36
2514
326.2
36.64
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.045 ± 0.033
0.885 ± 0.012
5.123 ± 0.027
5.734 ± 0.039
4.956 ± 0.03
6.884 ± 0.038
1.987 ± 0.015
7.268 ± 0.033
6.938 ± 0.037
9.423 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.409 ± 0.018
5.533 ± 0.031
3.939 ± 0.024
3.815 ± 0.026
4.035 ± 0.024
6.573 ± 0.036
5.733 ± 0.037
6.39 ± 0.028
1.28 ± 0.013
4.05 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here