Shuangao sobemo-like virus 1

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEE6|A0A1L3KEE6_9VIRU RNA-directed RNA polymerase OS=Shuangao sobemo-like virus 1 OX=1923474 PE=4 SV=1
MM1 pKa = 7.67RR2 pKa = 11.84SIAAHH7 pKa = 5.73AGLRR11 pKa = 11.84EE12 pKa = 4.06GLSDD16 pKa = 4.32GPCNSIRR23 pKa = 11.84TKK25 pKa = 10.23MIQTVIEE32 pKa = 4.28RR33 pKa = 11.84NMAAQWEE40 pKa = 4.42IPPDD44 pKa = 3.71FLEE47 pKa = 4.36RR48 pKa = 11.84THH50 pKa = 7.0FDD52 pKa = 3.02RR53 pKa = 11.84VVAAIDD59 pKa = 3.51MTSTPGYY66 pKa = 9.3PYY68 pKa = 10.12IRR70 pKa = 11.84SATTNRR76 pKa = 11.84QYY78 pKa = 11.53FNADD82 pKa = 3.32DD83 pKa = 3.76PVKK86 pKa = 10.61FRR88 pKa = 11.84EE89 pKa = 4.29TCDD92 pKa = 3.79AIYY95 pKa = 8.46EE96 pKa = 4.49TVCAKK101 pKa = 10.09IDD103 pKa = 3.39GRR105 pKa = 11.84MGADD109 pKa = 4.18HH110 pKa = 7.0IRR112 pKa = 11.84LFIKK116 pKa = 10.69GEE118 pKa = 3.98PLPQHH123 pKa = 6.1KK124 pKa = 10.59VEE126 pKa = 4.19EE127 pKa = 4.47GRR129 pKa = 11.84LRR131 pKa = 11.84LISSVSVVDD140 pKa = 4.17QIVDD144 pKa = 3.41AMLHH148 pKa = 5.66GACNEE153 pKa = 3.84ALKK156 pKa = 10.49EE157 pKa = 3.68KK158 pKa = 10.16HH159 pKa = 6.67AYY161 pKa = 7.01VTPKK165 pKa = 10.62VGWSPYY171 pKa = 8.98GGGWKK176 pKa = 9.94IIPKK180 pKa = 8.49EE181 pKa = 3.52GWMALDD187 pKa = 3.67KK188 pKa = 11.03KK189 pKa = 10.79AWDD192 pKa = 3.51WSVKK196 pKa = 9.55PWILEE201 pKa = 3.81AEE203 pKa = 4.16LEE205 pKa = 4.1IRR207 pKa = 11.84IGLCTNINEE216 pKa = 4.31KK217 pKa = 9.84WLTLARR223 pKa = 11.84KK224 pKa = 9.48RR225 pKa = 11.84YY226 pKa = 7.67EE227 pKa = 3.8QLFVEE232 pKa = 5.43PIFVTSGGLLLQQIEE247 pKa = 4.21GGIIKK252 pKa = 10.31SGMVCTITTNCFGQDD267 pKa = 2.83ITHH270 pKa = 5.77VTTCYY275 pKa = 10.59TNGLLADD282 pKa = 4.18WLYY285 pKa = 12.13SMGDD289 pKa = 3.62DD290 pKa = 3.55TLQPPLEE297 pKa = 4.32EE298 pKa = 4.39PEE300 pKa = 3.88RR301 pKa = 11.84TEE303 pKa = 3.93YY304 pKa = 11.33LEE306 pKa = 4.23TLSQYY311 pKa = 10.48CHH313 pKa = 6.82IKK315 pKa = 10.34PGVGTNEE322 pKa = 3.85FAGFRR327 pKa = 11.84YY328 pKa = 10.06GRR330 pKa = 11.84GIEE333 pKa = 3.82PLYY336 pKa = 10.33RR337 pKa = 11.84PRR339 pKa = 11.84HH340 pKa = 5.59AYY342 pKa = 9.41QLLHH346 pKa = 6.75FDD348 pKa = 4.66DD349 pKa = 5.21RR350 pKa = 11.84YY351 pKa = 10.86KK352 pKa = 10.86EE353 pKa = 3.9QLAASYY359 pKa = 9.29TLLYY363 pKa = 10.1HH364 pKa = 7.61RR365 pKa = 11.84SSCRR369 pKa = 11.84NWMEE373 pKa = 4.27KK374 pKa = 10.46FFTEE378 pKa = 5.14LGISFPARR386 pKa = 11.84NIRR389 pKa = 11.84DD390 pKa = 3.94IIWDD394 pKa = 3.65SEE396 pKa = 4.21EE397 pKa = 3.76

Molecular weight: 45.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEE6|A0A1L3KEE6_9VIRU RNA-directed RNA polymerase OS=Shuangao sobemo-like virus 1 OX=1923474 PE=4 SV=1
MM1 pKa = 7.66LNALWNQRR9 pKa = 11.84KK10 pKa = 9.09LFVVGITLYY19 pKa = 10.9LVLSGWSGLPLHH31 pKa = 6.04TLVFRR36 pKa = 11.84TVRR39 pKa = 11.84YY40 pKa = 8.85LVWKK44 pKa = 9.9YY45 pKa = 9.02VLRR48 pKa = 11.84DD49 pKa = 4.05TIAGQFLEE57 pKa = 4.43EE58 pKa = 4.58LSSEE62 pKa = 4.13ILGKK66 pKa = 10.27EE67 pKa = 4.01MPPPPPPPPPKK78 pKa = 9.96PIPPLTDD85 pKa = 2.82RR86 pKa = 11.84LYY88 pKa = 10.88EE89 pKa = 4.04DD90 pKa = 3.97WIGLYY95 pKa = 10.55GRR97 pKa = 11.84TVNSTTRR104 pKa = 11.84VLEE107 pKa = 3.8KK108 pKa = 10.28AYY110 pKa = 10.48YY111 pKa = 10.21SSWTSWFHH119 pKa = 5.09MACNTIAIIVAIRR132 pKa = 11.84TLFVAISRR140 pKa = 11.84IIPGSPRR147 pKa = 11.84TAVATWAKK155 pKa = 10.01CKK157 pKa = 10.04IRR159 pKa = 11.84GIHH162 pKa = 5.8YY163 pKa = 7.45EE164 pKa = 3.77AAIQGSLLRR173 pKa = 11.84KK174 pKa = 8.41ATIPEE179 pKa = 3.99WQVEE183 pKa = 4.3VLTPGFLSDD192 pKa = 3.23THH194 pKa = 7.11VGWGVRR200 pKa = 11.84VVDD203 pKa = 3.71TLVVPTHH210 pKa = 5.88VLVEE214 pKa = 4.43AGPNYY219 pKa = 10.21LLKK222 pKa = 10.91GRR224 pKa = 11.84TGSVLCNGLPRR235 pKa = 11.84DD236 pKa = 3.81SRR238 pKa = 11.84IMQDD242 pKa = 2.42ISYY245 pKa = 10.39VPVEE249 pKa = 3.82VSTWSKK255 pKa = 11.05LGTPSVKK262 pKa = 9.9RR263 pKa = 11.84KK264 pKa = 10.08RR265 pKa = 11.84IDD267 pKa = 3.25VEE269 pKa = 4.51TVQSVSCCGLEE280 pKa = 4.33GASTGPLRR288 pKa = 11.84QTRR291 pKa = 11.84VRR293 pKa = 11.84GLMYY297 pKa = 10.83YY298 pKa = 10.1EE299 pKa = 4.59GTTMPGMSGAGYY311 pKa = 10.51YY312 pKa = 9.7IGNTCLGIHH321 pKa = 6.85LGASTNVNCGATIMVPFAEE340 pKa = 4.7IPAPNNLVVQVEE352 pKa = 4.81RR353 pKa = 11.84SAKK356 pKa = 9.56RR357 pKa = 11.84PKK359 pKa = 9.1KK360 pKa = 8.51TRR362 pKa = 11.84PPSGTSADD370 pKa = 3.89PTYY373 pKa = 11.02HH374 pKa = 7.78AMDD377 pKa = 3.57NTKK380 pKa = 8.8TAWGYY385 pKa = 11.02ADD387 pKa = 3.68IRR389 pKa = 11.84EE390 pKa = 4.4ALDD393 pKa = 4.1DD394 pKa = 3.89YY395 pKa = 11.73DD396 pKa = 5.56RR397 pKa = 11.84FIGPSDD403 pKa = 3.48WNNPTDD409 pKa = 5.34DD410 pKa = 4.12FDD412 pKa = 5.13YY413 pKa = 11.27DD414 pKa = 3.17QDD416 pKa = 3.59LGYY419 pKa = 10.6EE420 pKa = 4.23YY421 pKa = 10.62EE422 pKa = 4.3EE423 pKa = 4.27EE424 pKa = 3.92AATRR428 pKa = 11.84GNTLMEE434 pKa = 4.49QIRR437 pKa = 11.84RR438 pKa = 11.84MSVEE442 pKa = 4.26DD443 pKa = 3.28RR444 pKa = 11.84KK445 pKa = 11.09LWMDD449 pKa = 4.6SINAVATEE457 pKa = 4.0EE458 pKa = 4.06AVAARR463 pKa = 11.84STPVANPLPSVRR475 pKa = 11.84PMVQTRR481 pKa = 11.84QGVPRR486 pKa = 11.84QVTFDD491 pKa = 3.86EE492 pKa = 5.24IIVHH496 pKa = 5.62NQTGVGTSTTIHH508 pKa = 6.0TQPQPQRR515 pKa = 11.84LAGPPHH521 pKa = 7.12LGDD524 pKa = 4.74LVRR527 pKa = 11.84DD528 pKa = 4.22LIKK531 pKa = 10.51RR532 pKa = 11.84VTILEE537 pKa = 3.86QKK539 pKa = 9.76MIQRR543 pKa = 11.84DD544 pKa = 3.42EE545 pKa = 4.8RR546 pKa = 11.84MVEE549 pKa = 3.6IEE551 pKa = 4.15NAVGTLQDD559 pKa = 3.45AVAKK563 pKa = 10.01YY564 pKa = 9.93GSPEE568 pKa = 3.84EE569 pKa = 4.16LEE571 pKa = 4.15KK572 pKa = 11.12LRR574 pKa = 11.84NDD576 pKa = 3.49LSGYY580 pKa = 8.25RR581 pKa = 11.84HH582 pKa = 5.82TMQEE586 pKa = 3.69IAADD590 pKa = 4.0ACIMTQDD597 pKa = 3.08QVGYY601 pKa = 8.81FRR603 pKa = 11.84CEE605 pKa = 3.45QCGKK609 pKa = 9.9VLHH612 pKa = 6.16SQYY615 pKa = 11.34DD616 pKa = 3.93LNEE619 pKa = 3.85HH620 pKa = 6.97RR621 pKa = 11.84ISVHH625 pKa = 3.51KK626 pKa = 9.38WKK628 pKa = 10.21RR629 pKa = 11.84VSKK632 pKa = 10.2GRR634 pKa = 11.84PTGSCSIASEE644 pKa = 4.07IYY646 pKa = 10.23GPKK649 pKa = 9.84EE650 pKa = 3.71NPPKK654 pKa = 10.13EE655 pKa = 4.4EE656 pKa = 4.48KK657 pKa = 10.09PVSQPQRR664 pKa = 11.84NRR666 pKa = 11.84RR667 pKa = 11.84EE668 pKa = 3.85VHH670 pKa = 5.61EE671 pKa = 4.61EE672 pKa = 3.84EE673 pKa = 4.87LEE675 pKa = 3.93KK676 pKa = 10.89ALPYY680 pKa = 10.65KK681 pKa = 10.33CTDD684 pKa = 3.23CNRR687 pKa = 11.84RR688 pKa = 11.84FATEE692 pKa = 3.98SARR695 pKa = 11.84LAHH698 pKa = 6.79FGAVHH703 pKa = 7.57AIHH706 pKa = 7.13IAPEE710 pKa = 4.13SAIPTDD716 pKa = 3.34SQDD719 pKa = 3.29PVKK722 pKa = 10.14TDD724 pKa = 3.29RR725 pKa = 11.84AFLGQRR731 pKa = 11.84SRR733 pKa = 11.84LRR735 pKa = 11.84KK736 pKa = 8.76QRR738 pKa = 11.84RR739 pKa = 11.84TKK741 pKa = 10.77SADD744 pKa = 3.21TSVSLAGQTLLQSLQAGQSEE764 pKa = 4.43ISHH767 pKa = 6.77CLQSVSRR774 pKa = 11.84SLNEE778 pKa = 3.75LQKK781 pKa = 10.83VIHH784 pKa = 6.24GRR786 pKa = 11.84NSGTTQKK793 pKa = 11.33

Molecular weight: 88.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1190

397

793

595.0

66.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.723 ± 0.095

2.017 ± 0.276

4.79 ± 0.275

6.723 ± 0.597

2.353 ± 0.645

6.975 ± 0.181

2.605 ± 0.091

6.303 ± 0.828

4.538 ± 0.136

8.151 ± 0.089

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.269 ± 0.137

3.193 ± 0.094

6.134 ± 0.603

4.202 ± 0.51

7.143 ± 0.326

6.218 ± 0.787

7.227 ± 0.372

6.723 ± 1.203

2.101 ± 0.368

3.613 ± 0.367
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski