Pseudoalteromonas phage pYD6-A

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Schitoviridae; Fuhrmanvirinae; Matsuvirus; Pseudoalteromonas virus pYD6A

Average proteome isoelectric point is 5.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 98 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M4SMF5|M4SMF5_9CAUD Uncharacterized protein OS=Pseudoalteromonas phage pYD6-A OX=754052 GN=PYDG_00015 PE=4 SV=1
MM1 pKa = 7.07QVHH4 pKa = 7.14LYY6 pKa = 10.5KK7 pKa = 10.84VVDD10 pKa = 3.98EE11 pKa = 4.85NNDD14 pKa = 3.19VIYY17 pKa = 10.55ILMGEE22 pKa = 4.58EE23 pKa = 4.58GWSSYY28 pKa = 10.58EE29 pKa = 3.99PSNNDD34 pKa = 3.07PSVNNLTNIILSINDD49 pKa = 3.44TQFPPEE55 pKa = 3.91TAFEE59 pKa = 4.18YY60 pKa = 10.4SDD62 pKa = 3.82KK63 pKa = 11.03SVEE66 pKa = 4.42LILIDD71 pKa = 3.92VPFDD75 pKa = 3.35VAFDD79 pKa = 3.86YY80 pKa = 11.4ARR82 pKa = 11.84GLSLLDD88 pKa = 3.48

Molecular weight: 10.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M4SNE6|M4SNE6_9CAUD Uncharacterized protein OS=Pseudoalteromonas phage pYD6-A OX=754052 GN=PYDG_00031 PE=4 SV=1
MM1 pKa = 8.04KK2 pKa = 9.98IRR4 pKa = 11.84KK5 pKa = 8.65RR6 pKa = 11.84CKK8 pKa = 9.83LRR10 pKa = 11.84SQDD13 pKa = 4.21RR14 pKa = 11.84AWQLFWTAQKK24 pKa = 10.27EE25 pKa = 4.19QYY27 pKa = 10.5KK28 pKa = 9.69VIKK31 pKa = 10.34VRR33 pKa = 11.84IIVEE37 pKa = 3.93NLSNYY42 pKa = 8.05STGRR46 pKa = 11.84PRR48 pKa = 11.84KK49 pKa = 9.24VINHH53 pKa = 6.76RR54 pKa = 11.84SQLVLTPKK62 pKa = 10.41GNSYY66 pKa = 11.2NLLCMITGDD75 pKa = 3.84QPLLEE80 pKa = 4.3TFTWSFTNTHH90 pKa = 6.72KK91 pKa = 10.84NRR93 pKa = 11.84QDD95 pKa = 3.42YY96 pKa = 10.83VLL98 pKa = 4.65

Molecular weight: 11.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

98

0

98

23211

44

3264

236.8

26.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.686 ± 0.424

0.926 ± 0.151

6.85 ± 0.235

7.053 ± 0.264

3.796 ± 0.165

6.669 ± 0.285

1.887 ± 0.131

6.269 ± 0.214

6.518 ± 0.288

8.229 ± 0.274

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.675 ± 0.136

5.743 ± 0.278

3.464 ± 0.148

3.925 ± 0.24

4.179 ± 0.161

6.2 ± 0.207

6.389 ± 0.241

6.338 ± 0.203

1.361 ± 0.119

3.843 ± 0.234
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski