Salmonella phage Astrid

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Astrithrvirus; unclassified Astrithrvirus

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 15 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G8F2F9|A0A3G8F2F9_9CAUD Uncharacterized protein OS=Salmonella phage Astrid OX=2483851 PE=4 SV=1
MM1 pKa = 7.36ANVYY5 pKa = 10.32RR6 pKa = 11.84FTQADD11 pKa = 3.05IDD13 pKa = 3.7AAAAQGVTMKK23 pKa = 10.95LSGLDD28 pKa = 3.3VSDD31 pKa = 4.56FDD33 pKa = 5.05GFSDD37 pKa = 4.6SDD39 pKa = 4.01TLVATASGGRR49 pKa = 11.84KK50 pKa = 7.57FTVIPASGFDD60 pKa = 3.5PAYY63 pKa = 8.61TSIYY67 pKa = 9.53FFGWNPSEE75 pKa = 4.07GEE77 pKa = 3.92NMNISFVLSNNDD89 pKa = 2.7TVATLKK95 pKa = 10.52PAVGMNFTKK104 pKa = 10.46FFVTTQAAAVALAWVNHH121 pKa = 6.23PSSGSEE127 pKa = 3.51NTDD130 pKa = 3.29IVFSWDD136 pKa = 3.07GGTLTTGNYY145 pKa = 9.52NVVVTKK151 pKa = 10.86DD152 pKa = 3.04GAEE155 pKa = 4.09VVNISTPDD163 pKa = 3.05KK164 pKa = 11.13SYY166 pKa = 9.62TLNKK170 pKa = 8.68TAGDD174 pKa = 4.1YY175 pKa = 11.08VVTVYY180 pKa = 11.22DD181 pKa = 3.91KK182 pKa = 11.42GGKK185 pKa = 9.17SDD187 pKa = 3.65STASNISQAITLSAVLPKK205 pKa = 10.6ALYY208 pKa = 8.3TVKK211 pKa = 10.62AADD214 pKa = 3.16ITAISDD220 pKa = 3.5NEE222 pKa = 3.46IDD224 pKa = 3.77MKK226 pKa = 11.67VNGADD231 pKa = 2.97ITAGSVLRR239 pKa = 11.84LGDD242 pKa = 3.65VIVAKK247 pKa = 10.85VSGIRR252 pKa = 11.84KK253 pKa = 9.29FYY255 pKa = 10.85TDD257 pKa = 3.33TTLHH261 pKa = 5.37GTSINFAVFFDD272 pKa = 5.81GEE274 pKa = 4.65SQWLQFALSDD284 pKa = 3.71NDD286 pKa = 3.37QTATFTMVDD295 pKa = 3.97DD296 pKa = 4.42TSGSAGTYY304 pKa = 6.73QAWNIRR310 pKa = 11.84TKK312 pKa = 10.57QEE314 pKa = 3.66TPAVVGTNNVYY325 pKa = 10.66KK326 pKa = 9.93IDD328 pKa = 3.8TSILSSVNKK337 pKa = 9.76EE338 pKa = 3.73RR339 pKa = 11.84FVTITGSDD347 pKa = 3.39TPFDD351 pKa = 3.8YY352 pKa = 10.84GQYY355 pKa = 9.88ILSVLQFPFDD365 pKa = 3.58IPADD369 pKa = 3.67QILTPEE375 pKa = 4.73NIQLANRR382 pKa = 11.84QLSVAANKK390 pKa = 9.81VATDD394 pKa = 4.32KK395 pKa = 11.22IKK397 pKa = 10.42IDD399 pKa = 3.4LGEE402 pKa = 4.01IVVPDD407 pKa = 3.77TYY409 pKa = 11.91GNMLSFANTNAVIHH423 pKa = 6.43LPLAPSIVLDD433 pKa = 3.7LEE435 pKa = 4.46YY436 pKa = 11.1VIGQTLGVYY445 pKa = 10.19YY446 pKa = 10.95LLDD449 pKa = 4.86CYY451 pKa = 10.79TGTATINITSTKK463 pKa = 8.71LAAVISSTQVNIGVRR478 pKa = 11.84VPYY481 pKa = 9.89MADD484 pKa = 2.97SYY486 pKa = 10.14TAPEE490 pKa = 4.06NTGVVAGGNNGVKK503 pKa = 9.69IPYY506 pKa = 9.48IEE508 pKa = 5.51LISHH512 pKa = 7.26DD513 pKa = 5.09AILPHH518 pKa = 6.82GFFTVPVVDD527 pKa = 3.68EE528 pKa = 4.36TLISGQTGYY537 pKa = 10.64IKK539 pKa = 10.53VDD541 pKa = 3.2NVEE544 pKa = 4.44LVTGALGNEE553 pKa = 4.02KK554 pKa = 10.56AQIISLLNSGVIIKK568 pKa = 10.32

Molecular weight: 60.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G8F4Y3|A0A3G8F4Y3_9CAUD DNA-directed DNA polymerase OS=Salmonella phage Astrid OX=2483851 PE=4 SV=1
MM1 pKa = 7.88GINITRR7 pKa = 11.84QRR9 pKa = 11.84LLDD12 pKa = 3.53GMRR15 pKa = 11.84SIGEE19 pKa = 4.23PFTQSEE25 pKa = 4.33IEE27 pKa = 4.47SICRR31 pKa = 11.84EE32 pKa = 4.01LFHH35 pKa = 7.08NRR37 pKa = 11.84GKK39 pKa = 10.51ISDD42 pKa = 4.49LKK44 pKa = 11.19NKK46 pKa = 9.57LASKK50 pKa = 10.39SDD52 pKa = 3.5NKK54 pKa = 10.32KK55 pKa = 10.83APP57 pKa = 3.43

Molecular weight: 6.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

15

0

15

3632

57

708

242.1

27.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.828 ± 0.805

0.798 ± 0.222

6.635 ± 0.403

6.47 ± 0.8

4.268 ± 0.249

6.333 ± 0.396

1.377 ± 0.319

6.718 ± 0.361

7.461 ± 0.725

7.764 ± 0.429

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.029 ± 0.347

6.856 ± 0.639

2.671 ± 0.248

3.139 ± 0.595

3.111 ± 0.514

6.525 ± 0.417

7.048 ± 0.782

7.241 ± 0.818

0.964 ± 0.132

4.763 ± 0.784
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski