Agromyces sp. SJ-23

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Agromyces

Average proteome isoelectric point is 5.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3729 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3M7ZVZ5|A0A3M7ZVZ5_9MICO ATP-binding protein (Fragment) OS=Agromyces sp. SJ-23 OX=2583849 GN=EDM22_19280 PE=4 SV=1
MM1 pKa = 8.04RR2 pKa = 11.84FPIHH6 pKa = 6.33VPAAVDD12 pKa = 3.32DD13 pKa = 3.83RR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84PVRR19 pKa = 11.84LRR21 pKa = 11.84LAALGVATILAAGGAFATPFAAHH44 pKa = 6.82AEE46 pKa = 4.23EE47 pKa = 4.41AQPAGGEE54 pKa = 4.05SAMLVEE60 pKa = 5.12AAASEE65 pKa = 4.28PVAAEE70 pKa = 4.19DD71 pKa = 4.38PAPADD76 pKa = 3.58EE77 pKa = 5.19GEE79 pKa = 4.47TGASATEE86 pKa = 4.14PAASATDD93 pKa = 3.88DD94 pKa = 4.13DD95 pKa = 4.95AATAHH100 pKa = 7.01ADD102 pKa = 3.47AAADD106 pKa = 3.75PAGPAAGAAGDD117 pKa = 4.41PEE119 pKa = 5.24ASHH122 pKa = 6.94LPDD125 pKa = 5.0AEE127 pKa = 4.39AEE129 pKa = 4.08PVAARR134 pKa = 11.84KK135 pKa = 9.06SAASAGQPAGFAAQVEE151 pKa = 4.45PSQLGPNQAPIAVDD165 pKa = 3.48DD166 pKa = 4.94AYY168 pKa = 11.88QMLADD173 pKa = 3.86TTLTVDD179 pKa = 4.1DD180 pKa = 5.21PGVTGNDD187 pKa = 3.72TEE189 pKa = 5.22PDD191 pKa = 3.7GDD193 pKa = 3.61WYY195 pKa = 11.18QVDD198 pKa = 4.63DD199 pKa = 3.83HH200 pKa = 6.8TKK202 pKa = 9.07PQVGSLWVNAHH213 pKa = 6.08GPFGYY218 pKa = 8.84TPPAGFTGTVTFTYY232 pKa = 10.6VLKK235 pKa = 10.74DD236 pKa = 3.44DD237 pKa = 5.17FGALSTWATVTIEE250 pKa = 3.96VLPAGSDD257 pKa = 3.08ATLPPTANDD266 pKa = 3.32DD267 pKa = 4.02TYY269 pKa = 11.75VYY271 pKa = 11.15ALSTPLYY278 pKa = 8.49IAAPGVLANDD288 pKa = 3.93DD289 pKa = 3.76AEE291 pKa = 4.38GRR293 pKa = 11.84EE294 pKa = 4.37GTLVLDD300 pKa = 4.06YY301 pKa = 10.89ASPQIGTVDD310 pKa = 3.92LQADD314 pKa = 4.26GSFLYY319 pKa = 10.01TPHH322 pKa = 7.55DD323 pKa = 4.84SIGGTWWFRR332 pKa = 11.84YY333 pKa = 8.86LLCTDD338 pKa = 4.54GGCASAEE345 pKa = 4.18VTLEE349 pKa = 3.57QAAAGEE355 pKa = 4.43QPSGPSQPPAEE366 pKa = 4.5PPAGEE371 pKa = 4.52DD372 pKa = 3.61LAPVAQPDD380 pKa = 3.88TLVAVAGDD388 pKa = 4.09LAILDD393 pKa = 4.01APGVLGNDD401 pKa = 4.01SDD403 pKa = 5.59PEE405 pKa = 4.3GQPLTLVDD413 pKa = 3.84VTTPAHH419 pKa = 5.44GVLYY423 pKa = 10.11YY424 pKa = 10.01WNADD428 pKa = 3.1GTVQYY433 pKa = 10.94VPNDD437 pKa = 3.38GFAGTDD443 pKa = 3.21QVEE446 pKa = 4.49YY447 pKa = 9.89TVSDD451 pKa = 3.98GAKK454 pKa = 9.85SATSTLTFTVTEE466 pKa = 4.13PANYY470 pKa = 9.98APQAWEE476 pKa = 4.1DD477 pKa = 3.74SAVAVAGTTLILDD490 pKa = 3.93APGVLGNDD498 pKa = 3.59SDD500 pKa = 4.62QDD502 pKa = 3.67GDD504 pKa = 4.01PLAVTWFGDD513 pKa = 3.64PHH515 pKa = 7.82HH516 pKa = 6.38GTIDD520 pKa = 3.13IAADD524 pKa = 3.64GSVVYY529 pKa = 9.65TPEE532 pKa = 5.05AGFVGGDD539 pKa = 3.34SVLYY543 pKa = 9.82QASDD547 pKa = 3.53GQATSEE553 pKa = 4.15AFLVIDD559 pKa = 4.24VVSAGAPAHH568 pKa = 5.38PTVVGDD574 pKa = 4.08HH575 pKa = 6.6YY576 pKa = 11.44DD577 pKa = 3.34AVSGVLLEE585 pKa = 4.31VSAPGVLGNDD595 pKa = 4.53LDD597 pKa = 4.19PSGPIAVTGHH607 pKa = 6.21EE608 pKa = 4.24AAQHH612 pKa = 4.41GTIEE616 pKa = 4.06IAADD620 pKa = 3.51GSLRR624 pKa = 11.84YY625 pKa = 8.14TSEE628 pKa = 3.82PGYY631 pKa = 9.62TGVDD635 pKa = 3.85TIRR638 pKa = 11.84YY639 pKa = 5.92TISDD643 pKa = 4.09GEE645 pKa = 4.12EE646 pKa = 4.05SADD649 pKa = 3.42GLISITVLGQAEE661 pKa = 4.8GSGQPGEE668 pKa = 4.5GEE670 pKa = 4.11QPGGSGGSGGPQQPGRR686 pKa = 11.84PQQPTGPDD694 pKa = 3.25EE695 pKa = 4.51PGRR698 pKa = 11.84PAEE701 pKa = 4.17HH702 pKa = 6.86APLPEE707 pKa = 4.47ARR709 pKa = 11.84PATTDD714 pKa = 3.11AAALADD720 pKa = 3.83TGSDD724 pKa = 3.32PRR726 pKa = 11.84LAAALAALLAALGII740 pKa = 4.44

Molecular weight: 74.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3M8A883|A0A3M8A883_9MICO PPOX class F420-dependent oxidoreductase OS=Agromyces sp. SJ-23 OX=2583849 GN=EDM22_12195 PE=4 SV=1
MM1 pKa = 7.96PLRR4 pKa = 11.84AARR7 pKa = 11.84VGRR10 pKa = 11.84VGVVGAPVAKK20 pKa = 10.13AAVVGAAVSPGRR32 pKa = 11.84SPVAKK37 pKa = 9.86AAVVGAAVTPGRR49 pKa = 11.84SPVAKK54 pKa = 9.86AAVVGAAVTPGRR66 pKa = 11.84RR67 pKa = 11.84RR68 pKa = 11.84RR69 pKa = 11.84II70 pKa = 3.53

Molecular weight: 6.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3729

0

3729

1218308

29

2207

326.7

34.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.335 ± 0.064

0.467 ± 0.009

6.257 ± 0.03

5.824 ± 0.03

3.153 ± 0.027

9.216 ± 0.033

2.005 ± 0.022

4.242 ± 0.028

1.619 ± 0.026

10.123 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.671 ± 0.015

1.767 ± 0.02

5.599 ± 0.028

2.49 ± 0.019

7.652 ± 0.052

5.295 ± 0.029

5.656 ± 0.034

9.152 ± 0.034

1.538 ± 0.018

1.939 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski