Streptomyces sp. Z26

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5304 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A498DNL0|A0A498DNL0_9ACTN Uncharacterized protein OS=Streptomyces sp. Z26 OX=2500177 GN=D7M15_10770 PE=4 SV=1
MM1 pKa = 7.02STGNEE6 pKa = 3.93ALEE9 pKa = 3.98VWIDD13 pKa = 3.41QDD15 pKa = 3.99LCTGDD20 pKa = 5.01GICAQYY26 pKa = 10.9APEE29 pKa = 4.23VFEE32 pKa = 5.9LDD34 pKa = 3.05IDD36 pKa = 3.63GLAYY40 pKa = 10.47VKK42 pKa = 10.9SGDD45 pKa = 5.18DD46 pKa = 4.27EE47 pKa = 4.88LLQDD51 pKa = 4.65KK52 pKa = 10.51GATTPVPLPLIRR64 pKa = 11.84DD65 pKa = 3.69VADD68 pKa = 3.59SAKK71 pKa = 9.94EE72 pKa = 3.91CPGDD76 pKa = 4.17CIHH79 pKa = 6.91VRR81 pKa = 11.84RR82 pKa = 11.84QSDD85 pKa = 3.62GVEE88 pKa = 4.34VYY90 pKa = 10.92GPDD93 pKa = 3.44ADD95 pKa = 3.69

Molecular weight: 10.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A498DUM8|A0A498DUM8_9ACTN Mechanosensitive ion channel family protein OS=Streptomyces sp. Z26 OX=2500177 GN=D7M15_07915 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIVANRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.81GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5304

0

5304

1814538

18

8259

342.1

36.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.147 ± 0.055

0.77 ± 0.009

6.386 ± 0.026

5.889 ± 0.029

2.563 ± 0.017

10.078 ± 0.036

2.335 ± 0.015

2.434 ± 0.023

1.813 ± 0.025

10.208 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.622 ± 0.013

1.54 ± 0.016

6.371 ± 0.036

2.466 ± 0.023

8.637 ± 0.044

4.691 ± 0.028

6.021 ± 0.024

8.585 ± 0.031

1.437 ± 0.012

2.006 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski