Acholeplasma phage MV-L51

Taxonomy: Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes; Tubulavirales; Plectroviridae; Plectrovirus; Acholeplasma virus L51

Average proteome isoelectric point is 8.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q04392|Q04392_9VIRU Uncharacterized protein OS=Acholeplasma phage MV-L51 OX=1977403 PE=4 SV=1
MM1 pKa = 7.82EE2 pKa = 5.31YY3 pKa = 10.21IEE5 pKa = 4.12ITVNKK10 pKa = 9.14SQHH13 pKa = 5.46FVNTDD18 pKa = 3.12QIYY21 pKa = 10.52NMLWSSAIMQCLISNEE37 pKa = 3.83YY38 pKa = 8.86HH39 pKa = 6.65HH40 pKa = 7.07NNNEE44 pKa = 3.76HH45 pKa = 5.94TSCINRR51 pKa = 11.84INRR54 pKa = 11.84NYY56 pKa = 10.49RR57 pKa = 11.84SNQRR61 pKa = 11.84HH62 pKa = 4.64HH63 pKa = 6.39QGYY66 pKa = 9.89NDD68 pKa = 4.83LYY70 pKa = 11.23DD71 pKa = 4.59SINIIQGMLEE81 pKa = 4.12NLNASIVYY89 pKa = 6.71FTKK92 pKa = 10.42DD93 pKa = 2.92GKK95 pKa = 11.09YY96 pKa = 10.55KK97 pKa = 11.02LIMTLL102 pKa = 3.53

Molecular weight: 12.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q04394|Q04394_9VIRU Uncharacterized protein OS=Acholeplasma phage MV-L51 OX=1977403 PE=4 SV=1
MM1 pKa = 7.53KK2 pKa = 10.04KK3 pKa = 9.23YY4 pKa = 10.73AKK6 pKa = 10.32LIFIFIMIIFIMNYY20 pKa = 9.95FIEE23 pKa = 4.51NADD26 pKa = 3.66AFRR29 pKa = 11.84LIIMEE34 pKa = 4.08VLQVQSTKK42 pKa = 10.77SFTIFIQLYY51 pKa = 8.58SFKK54 pKa = 10.83KK55 pKa = 9.96VINRR59 pKa = 11.84LCYY62 pKa = 9.63IFRR65 pKa = 11.84FLPFVSSLSLIYY77 pKa = 10.57LILSWRR83 pKa = 11.84IHH85 pKa = 6.56HH86 pKa = 6.45IVGHH90 pKa = 6.34KK91 pKa = 6.6MTKK94 pKa = 10.13SLLDD98 pKa = 3.36MSFSTWSLYY107 pKa = 10.66LGSILLYY114 pKa = 9.23TQVVWGLSLLIIAIIALYY132 pKa = 10.02FILKK136 pKa = 9.05FWWNNSQVTKK146 pKa = 10.99DD147 pKa = 3.27FTLYY151 pKa = 10.64NIHH154 pKa = 7.29IYY156 pKa = 10.02GAKK159 pKa = 9.61QRR161 pKa = 11.84GKK163 pKa = 10.47DD164 pKa = 3.4LTTQTIYY171 pKa = 10.96RR172 pKa = 11.84RR173 pKa = 11.84FHH175 pKa = 6.34KK176 pKa = 10.14EE177 pKa = 3.18YY178 pKa = 10.67NKK180 pKa = 10.42RR181 pKa = 11.84IKK183 pKa = 9.49QLKK186 pKa = 8.82NALKK190 pKa = 10.61LVFKK194 pKa = 10.92

Molecular weight: 23.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

725

102

266

181.3

21.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.414 ± 0.605

0.828 ± 0.263

2.621 ± 0.23

3.034 ± 0.927

5.103 ± 1.361

4.138 ± 0.651

2.897 ± 0.472

11.31 ± 0.949

8.828 ± 0.989

9.655 ± 1.504

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.621 ± 0.517

6.345 ± 1.323

1.793 ± 0.706

6.759 ± 1.382

3.448 ± 0.585

6.759 ± 0.767

6.897 ± 1.718

4.138 ± 0.209

1.379 ± 0.402

7.034 ± 0.994
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski