Mycoplasma sp. CAG:611

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae; Mycoplasma; environmental samples

Average proteome isoelectric point is 6.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 943 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5Y6Q4|R5Y6Q4_9MOLU Uncharacterized protein OS=Mycoplasma sp. CAG:611 OX=1262905 GN=BN735_00292 PE=4 SV=1
MM1 pKa = 7.84EE2 pKa = 6.62DD3 pKa = 3.6YY4 pKa = 11.23NIDD7 pKa = 3.78EE8 pKa = 4.51LLKK11 pKa = 10.51NIKK14 pKa = 9.84PNLHH18 pKa = 6.36KK19 pKa = 10.6SYY21 pKa = 11.29NGIFLTDD28 pKa = 3.55EE29 pKa = 4.14EE30 pKa = 5.09VSVLKK35 pKa = 10.93LYY37 pKa = 11.06GFDD40 pKa = 3.1INKK43 pKa = 10.08YY44 pKa = 9.77SDD46 pKa = 3.04IKK48 pKa = 11.18EE49 pKa = 4.09LMFDD53 pKa = 3.77LEE55 pKa = 4.58EE56 pKa = 4.07YY57 pKa = 11.13LNDD60 pKa = 3.75EE61 pKa = 4.33MQDD64 pKa = 3.68DD65 pKa = 4.73LEE67 pKa = 4.32QVLLSLAEE75 pKa = 3.89FNYY78 pKa = 10.61YY79 pKa = 11.21NNTTKK84 pKa = 10.96

Molecular weight: 10.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5Y9M6|R5Y9M6_9MOLU Uncharacterized protein OS=Mycoplasma sp. CAG:611 OX=1262905 GN=BN735_00774 PE=4 SV=1
MM1 pKa = 7.62KK2 pKa = 10.39KK3 pKa = 9.16GTFQPNNRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 9.5AKK15 pKa = 9.69KK16 pKa = 9.07HH17 pKa = 5.05GFFSRR22 pKa = 11.84MKK24 pKa = 10.33SGIVNKK30 pKa = 10.0RR31 pKa = 11.84RR32 pKa = 11.84AKK34 pKa = 10.05GRR36 pKa = 11.84KK37 pKa = 8.76KK38 pKa = 10.47LSKK41 pKa = 10.65

Molecular weight: 4.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

943

0

943

267452

30

1454

283.6

32.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.804 ± 0.07

1.167 ± 0.038

5.874 ± 0.065

7.302 ± 0.094

4.372 ± 0.068

5.083 ± 0.078

1.098 ± 0.029

10.441 ± 0.109

10.883 ± 0.092

9.456 ± 0.09

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.152 ± 0.033

8.269 ± 0.107

2.348 ± 0.04

1.738 ± 0.038

2.796 ± 0.058

5.961 ± 0.059

5.477 ± 0.081

5.988 ± 0.064

0.478 ± 0.018

5.313 ± 0.074
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski