Modestobacter sp. VKM Ac-2676

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Geodermatophilales; Geodermatophilaceae; Modestobacter; unclassified Modestobacter

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3187 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S8C8D3|A0A1S8C8D3_9ACTN Uncharacterized protein (Fragment) OS=Modestobacter sp. VKM Ac-2676 OX=1678130 GN=A7K94_0212885 PE=4 SV=1
MM1 pKa = 7.29SISWRR6 pKa = 11.84RR7 pKa = 11.84IAGAVLATALGTAALSGCAVTFIEE31 pKa = 5.22GTATPLAGARR41 pKa = 11.84DD42 pKa = 4.11DD43 pKa = 3.88VSPAEE48 pKa = 4.05FPITGAADD56 pKa = 3.69TQVDD60 pKa = 4.98RR61 pKa = 11.84IARR64 pKa = 11.84NALADD69 pKa = 4.45LNTWWAGQFGPVYY82 pKa = 9.59QQQFTPLSGGYY93 pKa = 9.94YY94 pKa = 10.26SVDD97 pKa = 3.51PADD100 pKa = 4.62IDD102 pKa = 3.55PTAYY106 pKa = 9.47PGGEE110 pKa = 4.12IGCGEE115 pKa = 4.2PPEE118 pKa = 4.64AVEE121 pKa = 6.18DD122 pKa = 3.64NAFYY126 pKa = 10.73CGPSQQYY133 pKa = 10.02RR134 pKa = 11.84NSDD137 pKa = 3.94AIQYY141 pKa = 10.28DD142 pKa = 3.82RR143 pKa = 11.84AFLDD147 pKa = 3.45EE148 pKa = 4.53LAFGTGGSEE157 pKa = 4.33GYY159 pKa = 10.84GRR161 pKa = 11.84FIPALVMAHH170 pKa = 6.2EE171 pKa = 5.39FGHH174 pKa = 6.49AVQGRR179 pKa = 11.84VGYY182 pKa = 8.95PFQASIAIEE191 pKa = 4.06TQADD195 pKa = 3.98CFAGAWTRR203 pKa = 11.84WVADD207 pKa = 3.4GEE209 pKa = 4.39APHH212 pKa = 6.6NSIRR216 pKa = 11.84PAEE219 pKa = 4.34LDD221 pKa = 3.5DD222 pKa = 3.8VLRR225 pKa = 11.84GYY227 pKa = 11.18LLLRR231 pKa = 11.84DD232 pKa = 3.93PVGTGLDD239 pKa = 3.13AGEE242 pKa = 4.09AHH244 pKa = 6.97GSYY247 pKa = 10.43FDD249 pKa = 3.76RR250 pKa = 11.84VSAFQEE256 pKa = 4.5GFDD259 pKa = 5.16DD260 pKa = 5.99GPTACRR266 pKa = 11.84DD267 pKa = 3.21GFGPDD272 pKa = 2.81RR273 pKa = 11.84PYY275 pKa = 10.4TQGAFRR281 pKa = 11.84DD282 pKa = 4.03DD283 pKa = 4.56DD284 pKa = 4.32DD285 pKa = 4.93ALSGGDD291 pKa = 3.5APYY294 pKa = 10.33EE295 pKa = 3.88QTVGQFLPEE304 pKa = 3.97GLGEE308 pKa = 4.19FWQQVFAEE316 pKa = 4.27RR317 pKa = 11.84GEE319 pKa = 4.35VFRR322 pKa = 11.84PPTLEE327 pKa = 4.23PFSGRR332 pKa = 11.84APSCDD337 pKa = 3.12GAGADD342 pKa = 4.03VDD344 pKa = 5.54LVYY347 pKa = 11.02CPDD350 pKa = 4.94DD351 pKa = 3.6DD352 pKa = 4.02TVAFDD357 pKa = 3.74EE358 pKa = 4.78AQLTEE363 pKa = 4.14PLYY366 pKa = 10.33EE367 pKa = 4.95AEE369 pKa = 4.41EE370 pKa = 4.84GGDD373 pKa = 3.78YY374 pKa = 11.27AVLTAVAIPYY384 pKa = 9.93GLAGRR389 pKa = 11.84DD390 pKa = 3.57QLGLSTDD397 pKa = 3.76GEE399 pKa = 4.63DD400 pKa = 3.67ALSSAVCLSGAFTASVLNEE419 pKa = 3.86EE420 pKa = 4.49STVLSISPGDD430 pKa = 3.44VDD432 pKa = 3.81EE433 pKa = 5.0SVSFLLEE440 pKa = 4.1YY441 pKa = 9.99ATDD444 pKa = 3.97PQVLPDD450 pKa = 3.62AQLTGFQLVDD460 pKa = 3.08VFRR463 pKa = 11.84TGVFEE468 pKa = 5.08GPAACALDD476 pKa = 3.53RR477 pKa = 4.83

Molecular weight: 50.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S8C9V5|A0A1S8C9V5_9ACTN Uncharacterized protein (Fragment) OS=Modestobacter sp. VKM Ac-2676 OX=1678130 GN=A7K94_0207295 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.99KK16 pKa = 9.77HH17 pKa = 5.81RR18 pKa = 11.84KK19 pKa = 8.51LLKK22 pKa = 8.15KK23 pKa = 9.25TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.81KK32 pKa = 9.63

Molecular weight: 4.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3187

0

3187

715476

18

1558

224.5

23.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.34 ± 0.062

0.744 ± 0.014

6.189 ± 0.04

5.591 ± 0.043

2.771 ± 0.026

9.603 ± 0.042

2.056 ± 0.023

3.133 ± 0.034

1.438 ± 0.032

10.379 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.806 ± 0.017

1.586 ± 0.02

6.039 ± 0.04

2.993 ± 0.028

8.022 ± 0.057

5.12 ± 0.037

6.238 ± 0.036

9.625 ± 0.046

1.496 ± 0.023

1.832 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski