Amino acid dipepetide frequency for Pseudoxanthomonas sp. CF125

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.427AlaAla: 16.427 ± 0.165
1.132AlaCys: 1.132 ± 0.035
6.904AlaAsp: 6.904 ± 0.081
6.893AlaGlu: 6.893 ± 0.086
4.053AlaPhe: 4.053 ± 0.066
10.641AlaGly: 10.641 ± 0.128
2.341AlaHis: 2.341 ± 0.051
5.442AlaIle: 5.442 ± 0.084
4.051AlaLys: 4.051 ± 0.074
14.18AlaLeu: 14.18 ± 0.134
3.184AlaMet: 3.184 ± 0.062
3.159AlaAsn: 3.159 ± 0.06
5.823AlaPro: 5.823 ± 0.08
5.141AlaGln: 5.141 ± 0.07
8.411AlaArg: 8.411 ± 0.106
6.956AlaSer: 6.956 ± 0.085
6.308AlaThr: 6.308 ± 0.105
8.448AlaVal: 8.448 ± 0.084
1.913AlaTrp: 1.913 ± 0.041
2.725AlaTyr: 2.725 ± 0.053
0.0AlaXaa: 0.0 ± 0.0
Cys
0.987CysAla: 0.987 ± 0.03
0.098CysCys: 0.098 ± 0.01
0.463CysAsp: 0.463 ± 0.023
0.45CysGlu: 0.45 ± 0.02
0.278CysPhe: 0.278 ± 0.013
0.871CysGly: 0.871 ± 0.027
0.199CysHis: 0.199 ± 0.013
0.35CysIle: 0.35 ± 0.017
0.237CysLys: 0.237 ± 0.014
0.775CysLeu: 0.775 ± 0.023
0.156CysMet: 0.156 ± 0.012
0.23CysAsn: 0.23 ± 0.014
0.376CysPro: 0.376 ± 0.019
0.213CysGln: 0.213 ± 0.014
0.533CysArg: 0.533 ± 0.02
0.466CysSer: 0.466 ± 0.02
0.457CysThr: 0.457 ± 0.026
0.605CysVal: 0.605 ± 0.025
0.109CysTrp: 0.109 ± 0.009
0.197CysTyr: 0.197 ± 0.013
0.0CysXaa: 0.0 ± 0.0
Asp
7.627AspAla: 7.627 ± 0.102
0.437AspCys: 0.437 ± 0.02
3.175AspAsp: 3.175 ± 0.052
3.244AspGlu: 3.244 ± 0.053
2.243AspPhe: 2.243 ± 0.045
5.514AspGly: 5.514 ± 0.075
1.174AspHis: 1.174 ± 0.031
2.442AspIle: 2.442 ± 0.045
1.784AspLys: 1.784 ± 0.041
5.402AspLeu: 5.402 ± 0.069
1.141AspMet: 1.141 ± 0.031
1.573AspAsn: 1.573 ± 0.041
3.36AspPro: 3.36 ± 0.05
1.768AspGln: 1.768 ± 0.038
3.796AspArg: 3.796 ± 0.058
2.938AspSer: 2.938 ± 0.054
2.829AspThr: 2.829 ± 0.052
3.806AspVal: 3.806 ± 0.065
1.101AspTrp: 1.101 ± 0.033
1.77AspTyr: 1.77 ± 0.039
0.0AspXaa: 0.0 ± 0.0
Glu
6.743GluAla: 6.743 ± 0.081
0.332GluCys: 0.332 ± 0.018
2.512GluAsp: 2.512 ± 0.049
2.777GluGlu: 2.777 ± 0.063
1.914GluPhe: 1.914 ± 0.048
4.174GluGly: 4.174 ± 0.055
1.262GluHis: 1.262 ± 0.031
2.759GluIle: 2.759 ± 0.049
1.87GluLys: 1.87 ± 0.042
5.902GluLeu: 5.902 ± 0.087
1.198GluMet: 1.198 ± 0.035
1.514GluAsn: 1.514 ± 0.037
2.492GluPro: 2.492 ± 0.04
2.537GluGln: 2.537 ± 0.054
4.699GluArg: 4.699 ± 0.066
2.991GluSer: 2.991 ± 0.054
2.919GluThr: 2.919 ± 0.056
3.991GluVal: 3.991 ± 0.067
0.848GluTrp: 0.848 ± 0.025
1.307GluTyr: 1.307 ± 0.034
0.0GluXaa: 0.0 ± 0.0
Phe
4.397PheAla: 4.397 ± 0.061
0.336PheCys: 0.336 ± 0.016
2.726PheAsp: 2.726 ± 0.046
1.985PheGlu: 1.985 ± 0.044
1.267PhePhe: 1.267 ± 0.034
3.53PheGly: 3.53 ± 0.062
0.799PheHis: 0.799 ± 0.028
1.385PheIle: 1.385 ± 0.037
1.094PheLys: 1.094 ± 0.031
3.235PheLeu: 3.235 ± 0.052
0.66PheMet: 0.66 ± 0.022
1.232PheAsn: 1.232 ± 0.037
1.512PhePro: 1.512 ± 0.037
0.989PheGln: 0.989 ± 0.029
2.102PheArg: 2.102 ± 0.039
2.27PheSer: 2.27 ± 0.047
1.855PheThr: 1.855 ± 0.043
2.61PheVal: 2.61 ± 0.041
0.538PheTrp: 0.538 ± 0.023
0.948PheTyr: 0.948 ± 0.029
0.0PheXaa: 0.0 ± 0.0
Gly
8.965GlyAla: 8.965 ± 0.122
0.775GlyCys: 0.775 ± 0.03
4.881GlyAsp: 4.881 ± 0.071
4.824GlyGlu: 4.824 ± 0.072
3.582GlyPhe: 3.582 ± 0.062
7.34GlyGly: 7.34 ± 0.11
1.838GlyHis: 1.838 ± 0.042
4.179GlyIle: 4.179 ± 0.06
3.739GlyLys: 3.739 ± 0.064
8.601GlyLeu: 8.601 ± 0.093
2.258GlyMet: 2.258 ± 0.043
2.783GlyAsn: 2.783 ± 0.06
3.133GlyPro: 3.133 ± 0.055
3.153GlyGln: 3.153 ± 0.05
5.573GlyArg: 5.573 ± 0.068
5.138GlySer: 5.138 ± 0.088
4.638GlyThr: 4.638 ± 0.083
6.367GlyVal: 6.367 ± 0.073
1.619GlyTrp: 1.619 ± 0.034
2.601GlyTyr: 2.601 ± 0.052
0.0GlyXaa: 0.0 ± 0.0
His
2.73HisAla: 2.73 ± 0.053
0.234HisCys: 0.234 ± 0.014
1.181HisAsp: 1.181 ± 0.033
1.117HisGlu: 1.117 ± 0.033
0.855HisPhe: 0.855 ± 0.025
2.118HisGly: 2.118 ± 0.044
0.532HisHis: 0.532 ± 0.021
0.791HisIle: 0.791 ± 0.024
0.494HisLys: 0.494 ± 0.02
2.028HisLeu: 2.028 ± 0.046
0.416HisMet: 0.416 ± 0.017
0.51HisAsn: 0.51 ± 0.021
1.325HisPro: 1.325 ± 0.038
0.619HisGln: 0.619 ± 0.022
1.506HisArg: 1.506 ± 0.034
1.077HisSer: 1.077 ± 0.033
0.929HisThr: 0.929 ± 0.026
1.382HisVal: 1.382 ± 0.032
0.425HisTrp: 0.425 ± 0.018
0.614HisTyr: 0.614 ± 0.023
0.0HisXaa: 0.0 ± 0.0
Ile
6.76IleAla: 6.76 ± 0.074
0.38IleCys: 0.38 ± 0.017
3.357IleAsp: 3.357 ± 0.052
3.044IleGlu: 3.044 ± 0.056
1.258IlePhe: 1.258 ± 0.032
4.635IleGly: 4.635 ± 0.078
0.817IleHis: 0.817 ± 0.029
1.403IleIle: 1.403 ± 0.039
1.305IleLys: 1.305 ± 0.037
3.2IleLeu: 3.2 ± 0.057
0.58IleMet: 0.58 ± 0.025
1.359IleAsn: 1.359 ± 0.043
2.26IlePro: 2.26 ± 0.04
1.135IleGln: 1.135 ± 0.031
2.693IleArg: 2.693 ± 0.052
2.433IleSer: 2.433 ± 0.043
2.248IleThr: 2.248 ± 0.046
3.395IleVal: 3.395 ± 0.055
0.49IleTrp: 0.49 ± 0.021
0.942IleTyr: 0.942 ± 0.027
0.0IleXaa: 0.0 ± 0.0
Lys
3.627LysAla: 3.627 ± 0.063
0.171LysCys: 0.171 ± 0.013
1.625LysAsp: 1.625 ± 0.04
1.45LysGlu: 1.45 ± 0.037
0.991LysPhe: 0.991 ± 0.028
2.313LysGly: 2.313 ± 0.049
0.721LysHis: 0.721 ± 0.028
1.495LysIle: 1.495 ± 0.035
1.347LysLys: 1.347 ± 0.054
3.709LysLeu: 3.709 ± 0.05
0.736LysMet: 0.736 ± 0.026
0.864LysAsn: 0.864 ± 0.025
2.341LysPro: 2.341 ± 0.05
1.45LysGln: 1.45 ± 0.04
2.504LysArg: 2.504 ± 0.055
1.811LysSer: 1.811 ± 0.038
1.849LysThr: 1.849 ± 0.042
2.374LysVal: 2.374 ± 0.053
0.389LysTrp: 0.389 ± 0.019
0.797LysTyr: 0.797 ± 0.026
0.0LysXaa: 0.0 ± 0.0
Leu
13.7LeuAla: 13.7 ± 0.129
0.922LeuCys: 0.922 ± 0.031
6.15LeuAsp: 6.15 ± 0.077
5.373LeuGlu: 5.373 ± 0.078
3.526LeuPhe: 3.526 ± 0.067
8.334LeuGly: 8.334 ± 0.094
2.245LeuHis: 2.245 ± 0.051
4.233LeuIle: 4.233 ± 0.059
3.389LeuLys: 3.389 ± 0.056
11.331LeuLeu: 11.331 ± 0.14
2.195LeuMet: 2.195 ± 0.048
2.563LeuAsn: 2.563 ± 0.05
6.238LeuPro: 6.238 ± 0.077
4.082LeuGln: 4.082 ± 0.067
8.052LeuArg: 8.052 ± 0.106
6.496LeuSer: 6.496 ± 0.09
5.113LeuThr: 5.113 ± 0.081
7.347LeuVal: 7.347 ± 0.096
1.431LeuTrp: 1.431 ± 0.043
2.264LeuTyr: 2.264 ± 0.047
0.0LeuXaa: 0.0 ± 0.0
Met
2.602MetAla: 2.602 ± 0.048
0.138MetCys: 0.138 ± 0.011
1.073MetAsp: 1.073 ± 0.027
1.052MetGlu: 1.052 ± 0.027
0.651MetPhe: 0.651 ± 0.027
1.613MetGly: 1.613 ± 0.039
0.436MetHis: 0.436 ± 0.021
0.92MetIle: 0.92 ± 0.029
0.916MetLys: 0.916 ± 0.031
2.351MetLeu: 2.351 ± 0.051
0.465MetMet: 0.465 ± 0.019
0.73MetAsn: 0.73 ± 0.022
1.375MetPro: 1.375 ± 0.036
0.914MetGln: 0.914 ± 0.028
1.711MetArg: 1.711 ± 0.038
1.573MetSer: 1.573 ± 0.037
1.386MetThr: 1.386 ± 0.033
1.424MetVal: 1.424 ± 0.037
0.224MetTrp: 0.224 ± 0.013
0.356MetTyr: 0.356 ± 0.017
0.0MetXaa: 0.0 ± 0.0
Asn
3.587AsnAla: 3.587 ± 0.063
0.234AsnCys: 0.234 ± 0.015
1.593AsnAsp: 1.593 ± 0.04
1.425AsnGlu: 1.425 ± 0.035
1.041AsnPhe: 1.041 ± 0.029
2.75AsnGly: 2.75 ± 0.064
0.528AsnHis: 0.528 ± 0.021
1.213AsnIle: 1.213 ± 0.03
0.774AsnLys: 0.774 ± 0.028
2.762AsnLeu: 2.762 ± 0.046
0.458AsnMet: 0.458 ± 0.021
0.836AsnAsn: 0.836 ± 0.03
1.967AsnPro: 1.967 ± 0.046
0.883AsnGln: 0.883 ± 0.031
1.78AsnArg: 1.78 ± 0.032
1.468AsnSer: 1.468 ± 0.047
1.577AsnThr: 1.577 ± 0.044
2.012AsnVal: 2.012 ± 0.039
0.452AsnTrp: 0.452 ± 0.024
0.836AsnTyr: 0.836 ± 0.03
0.0AsnXaa: 0.0 ± 0.0
Pro
6.996ProAla: 6.996 ± 0.103
0.291ProCys: 0.291 ± 0.018
3.251ProAsp: 3.251 ± 0.054
3.379ProGlu: 3.379 ± 0.052
1.682ProPhe: 1.682 ± 0.034
4.619ProGly: 4.619 ± 0.065
1.052ProHis: 1.052 ± 0.033
2.004ProIle: 2.004 ± 0.038
1.582ProLys: 1.582 ± 0.036
5.26ProLeu: 5.26 ± 0.07
1.243ProMet: 1.243 ± 0.034
1.376ProAsn: 1.376 ± 0.037
2.681ProPro: 2.681 ± 0.067
2.163ProGln: 2.163 ± 0.041
3.017ProArg: 3.017 ± 0.045
2.906ProSer: 2.906 ± 0.058
2.473ProThr: 2.473 ± 0.048
4.136ProVal: 4.136 ± 0.065
0.797ProTrp: 0.797 ± 0.025
1.299ProTyr: 1.299 ± 0.031
0.0ProXaa: 0.0 ± 0.0
Gln
5.053GlnAla: 5.053 ± 0.081
0.259GlnCys: 0.259 ± 0.015
1.65GlnAsp: 1.65 ± 0.037
1.665GlnGlu: 1.665 ± 0.041
1.191GlnPhe: 1.191 ± 0.035
2.95GlnGly: 2.95 ± 0.052
0.788GlnHis: 0.788 ± 0.028
1.642GlnIle: 1.642 ± 0.028
1.125GlnLys: 1.125 ± 0.033
3.866GlnLeu: 3.866 ± 0.06
0.801GlnMet: 0.801 ± 0.028
0.899GlnAsn: 0.899 ± 0.031
2.07GlnPro: 2.07 ± 0.043
1.78GlnGln: 1.78 ± 0.053
3.333GlnArg: 3.333 ± 0.062
2.119GlnSer: 2.119 ± 0.043
1.864GlnThr: 1.864 ± 0.042
2.846GlnVal: 2.846 ± 0.04
0.653GlnTrp: 0.653 ± 0.025
0.885GlnTyr: 0.885 ± 0.025
0.0GlnXaa: 0.0 ± 0.0
Arg
7.487ArgAla: 7.487 ± 0.087
0.473ArgCys: 0.473 ± 0.019
4.192ArgAsp: 4.192 ± 0.058
4.469ArgGlu: 4.469 ± 0.074
2.877ArgPhe: 2.877 ± 0.055
4.945ArgGly: 4.945 ± 0.067
1.593ArgHis: 1.593 ± 0.039
3.928ArgIle: 3.928 ± 0.053
2.453ArgLys: 2.453 ± 0.048
7.602ArgLeu: 7.602 ± 0.096
1.836ArgMet: 1.836 ± 0.037
2.099ArgAsn: 2.099 ± 0.044
3.054ArgPro: 3.054 ± 0.048
2.74ArgGln: 2.74 ± 0.057
4.986ArgArg: 4.986 ± 0.078
3.717ArgSer: 3.717 ± 0.052
3.267ArgThr: 3.267 ± 0.047
4.884ArgVal: 4.884 ± 0.063
1.375ArgTrp: 1.375 ± 0.035
2.248ArgTyr: 2.248 ± 0.043
0.0ArgXaa: 0.0 ± 0.0
Ser
6.787SerAla: 6.787 ± 0.091
0.417SerCys: 0.417 ± 0.018
3.163SerAsp: 3.163 ± 0.055
2.908SerGlu: 2.908 ± 0.049
2.097SerPhe: 2.097 ± 0.05
5.665SerGly: 5.665 ± 0.094
1.183SerHis: 1.183 ± 0.034
2.468SerIle: 2.468 ± 0.05
1.865SerLys: 1.865 ± 0.042
5.99SerLeu: 5.99 ± 0.077
1.231SerMet: 1.231 ± 0.032
1.666SerAsn: 1.666 ± 0.043
3.078SerPro: 3.078 ± 0.058
2.024SerGln: 2.024 ± 0.043
3.81SerArg: 3.81 ± 0.054
3.399SerSer: 3.399 ± 0.061
3.033SerThr: 3.033 ± 0.067
4.115SerVal: 4.115 ± 0.072
0.848SerTrp: 0.848 ± 0.027
1.536SerTyr: 1.536 ± 0.042
0.0SerXaa: 0.0 ± 0.0
Thr
6.291ThrAla: 6.291 ± 0.091
0.441ThrCys: 0.441 ± 0.022
2.789ThrAsp: 2.789 ± 0.057
2.403ThrGlu: 2.403 ± 0.049
1.691ThrPhe: 1.691 ± 0.039
4.97ThrGly: 4.97 ± 0.075
1.105ThrHis: 1.105 ± 0.032
2.169ThrIle: 2.169 ± 0.048
1.193ThrLys: 1.193 ± 0.034
6.174ThrLeu: 6.174 ± 0.089
0.947ThrMet: 0.947 ± 0.029
1.332ThrAsn: 1.332 ± 0.042
3.409ThrPro: 3.409 ± 0.071
1.792ThrGln: 1.792 ± 0.042
3.314ThrArg: 3.314 ± 0.049
2.645ThrSer: 2.645 ± 0.056
2.847ThrThr: 2.847 ± 0.081
4.11ThrVal: 4.11 ± 0.073
0.687ThrTrp: 0.687 ± 0.026
1.268ThrTyr: 1.268 ± 0.043
0.0ThrXaa: 0.0 ± 0.0
Val
9.031ValAla: 9.031 ± 0.106
0.626ValCys: 0.626 ± 0.021
4.181ValAsp: 4.181 ± 0.056
4.37ValGlu: 4.37 ± 0.063
2.63ValPhe: 2.63 ± 0.048
5.631ValGly: 5.631 ± 0.088
1.398ValHis: 1.398 ± 0.037
3.298ValIle: 3.298 ± 0.054
2.074ValLys: 2.074 ± 0.042
7.865ValLeu: 7.865 ± 0.087
1.537ValMet: 1.537 ± 0.038
2.108ValAsn: 2.108 ± 0.052
3.727ValPro: 3.727 ± 0.062
2.398ValGln: 2.398 ± 0.042
4.895ValArg: 4.895 ± 0.065
4.388ValSer: 4.388 ± 0.069
3.786ValThr: 3.786 ± 0.064
5.88ValVal: 5.88 ± 0.067
0.984ValTrp: 0.984 ± 0.031
1.672ValTyr: 1.672 ± 0.037
0.0ValXaa: 0.0 ± 0.0
Trp
1.32TrpAla: 1.32 ± 0.034
0.137TrpCys: 0.137 ± 0.011
0.691TrpAsp: 0.691 ± 0.024
0.643TrpGlu: 0.643 ± 0.025
0.629TrpPhe: 0.629 ± 0.023
0.983TrpGly: 0.983 ± 0.028
0.374TrpHis: 0.374 ± 0.017
0.734TrpIle: 0.734 ± 0.027
0.625TrpLys: 0.625 ± 0.025
2.107TrpLeu: 2.107 ± 0.05
0.454TrpMet: 0.454 ± 0.021
0.571TrpAsn: 0.571 ± 0.021
0.766TrpPro: 0.766 ± 0.027
0.736TrpGln: 0.736 ± 0.028
1.395TrpArg: 1.395 ± 0.034
0.985TrpSer: 0.985 ± 0.03
0.795TrpThr: 0.795 ± 0.029
0.979TrpVal: 0.979 ± 0.03
0.328TrpTrp: 0.328 ± 0.017
0.401TrpTyr: 0.401 ± 0.019
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.015TyrAla: 3.015 ± 0.051
0.215TyrCys: 0.215 ± 0.014
1.569TyrAsp: 1.569 ± 0.043
1.268TyrGlu: 1.268 ± 0.037
1.015TyrPhe: 1.015 ± 0.028
2.394TyrGly: 2.394 ± 0.048
0.496TyrHis: 0.496 ± 0.022
0.838TyrIle: 0.838 ± 0.027
0.646TyrLys: 0.646 ± 0.025
2.645TyrLeu: 2.645 ± 0.05
0.415TyrMet: 0.415 ± 0.018
0.774TyrAsn: 0.774 ± 0.03
1.22TyrPro: 1.22 ± 0.031
0.924TyrGln: 0.924 ± 0.028
2.114TyrArg: 2.114 ± 0.041
1.505TyrSer: 1.505 ± 0.04
1.404TyrThr: 1.404 ± 0.043
1.768TyrVal: 1.768 ± 0.035
0.441TyrTrp: 0.441 ± 0.022
0.759TyrTyr: 0.759 ± 0.025
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3691 proteins (1245779 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski