Dragonfly larvae associated circular virus-7
Average proteome isoelectric point is 7.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W5U7R2|W5U7R2_9VIRU ATP-dependent helicase Rep OS=Dragonfly larvae associated circular virus-7 OX=1454028 PE=3 SV=1
MM1 pKa = 7.5 ALILAKK7 pKa = 9.96 RR8 pKa = 11.84 VRR10 pKa = 11.84 RR11 pKa = 11.84 VCFTLFEE18 pKa = 4.32 EE19 pKa = 4.89 VPPEE23 pKa = 4.59 FEE25 pKa = 4.44 EE26 pKa = 4.54 EE27 pKa = 3.73 WMQYY31 pKa = 11.06 LLVGRR36 pKa = 11.84 EE37 pKa = 3.98 VCPATGRR44 pKa = 11.84 EE45 pKa = 3.86 HH46 pKa = 5.13 WQGYY50 pKa = 9.93 LEE52 pKa = 5.43 LKK54 pKa = 10.6 SQMLWTTVKK63 pKa = 10.45 GRR65 pKa = 11.84 LGDD68 pKa = 3.67 QVHH71 pKa = 6.83 LEE73 pKa = 4.19 EE74 pKa = 5.49 CNGSAQDD81 pKa = 3.93 NIVYY85 pKa = 10.14 CSKK88 pKa = 10.78 DD89 pKa = 3.64 GNWVEE94 pKa = 3.81 WGEE97 pKa = 4.44 CKK99 pKa = 10.7 VQGARR104 pKa = 11.84 SDD106 pKa = 3.84 LSSLRR111 pKa = 11.84 TDD113 pKa = 3.59 VLNHH117 pKa = 5.91 GVTVAEE123 pKa = 4.04 ILEE126 pKa = 4.58 ANPMTYY132 pKa = 9.44 HH133 pKa = 5.88 QYY135 pKa = 11.18 GRR137 pKa = 11.84 TLLALEE143 pKa = 4.36 DD144 pKa = 3.72 LRR146 pKa = 11.84 YY147 pKa = 10.48 SRR149 pKa = 11.84 VFRR152 pKa = 11.84 DD153 pKa = 4.04 CSAAPTVTWFWGPTGCGKK171 pKa = 9.57 SHH173 pKa = 6.88 EE174 pKa = 4.42 SRR176 pKa = 11.84 ARR178 pKa = 11.84 ALALAGGDD186 pKa = 3.07 RR187 pKa = 11.84 SKK189 pKa = 10.82 IYY191 pKa = 11.0 VLANHH196 pKa = 7.2 DD197 pKa = 4.0 KK198 pKa = 11.04 GWWDD202 pKa = 4.0 LYY204 pKa = 10.96 NLQEE208 pKa = 4.53 IVILDD213 pKa = 4.13 EE214 pKa = 4.41 LRR216 pKa = 11.84 ADD218 pKa = 3.66 QVPWGLLLQLTTNVEE233 pKa = 4.24 CFVPRR238 pKa = 11.84 RR239 pKa = 11.84 GRR241 pKa = 11.84 QPCPFVAKK249 pKa = 10.05 HH250 pKa = 5.41 VFITSPLHH258 pKa = 6.9 PRR260 pKa = 11.84 DD261 pKa = 3.61 QYY263 pKa = 12.01 ADD265 pKa = 3.28 RR266 pKa = 11.84 CGIDD270 pKa = 5.09 NIDD273 pKa = 3.32 QLLRR277 pKa = 11.84 RR278 pKa = 11.84 ITFIEE283 pKa = 4.18 EE284 pKa = 3.79 RR285 pKa = 11.84 NVPYY289 pKa = 10.47 AAGVFNHH296 pKa = 6.11 SKK298 pKa = 10.72 RR299 pKa = 11.84 LLFQGSMRR307 pKa = 11.84 TEE309 pKa = 3.9 RR310 pKa = 11.84 EE311 pKa = 3.71
Molecular weight: 35.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.937
IPC2_protein 5.957
IPC_protein 6.02
Toseland 6.275
ProMoST 6.249
Dawson 6.338
Bjellqvist 6.326
Wikipedia 6.351
Rodwell 6.326
Grimsley 6.376
Solomon 6.338
Lehninger 6.338
Nozaki 6.605
DTASelect 6.781
Thurlkill 6.81
EMBOSS 6.766
Sillero 6.722
Patrickios 4.088
IPC_peptide 6.351
IPC2_peptide 6.693
IPC2.peptide.svr19 6.627
Protein with the highest isoelectric point:
>tr|W5U7R2|W5U7R2_9VIRU ATP-dependent helicase Rep OS=Dragonfly larvae associated circular virus-7 OX=1454028 PE=3 SV=1
MM1 pKa = 7.47 SCRR4 pKa = 11.84 ACFYY8 pKa = 10.42 TFVGRR13 pKa = 11.84 NSGKK17 pKa = 9.98 VVNSLTLRR25 pKa = 11.84 RR26 pKa = 11.84 CLRR29 pKa = 11.84 VCVCVCVMAWGKK41 pKa = 9.16 YY42 pKa = 6.15 AHH44 pKa = 6.74 RR45 pKa = 11.84 RR46 pKa = 11.84 HH47 pKa = 5.72 YY48 pKa = 9.85 GHH50 pKa = 6.98 RR51 pKa = 11.84 RR52 pKa = 11.84 RR53 pKa = 11.84 YY54 pKa = 9.78 GRR56 pKa = 11.84 VNIQRR61 pKa = 11.84 NMLGMPSQRR70 pKa = 11.84 LVSLRR75 pKa = 11.84 QHH77 pKa = 6.28 NSWTQRR83 pKa = 11.84 MADD86 pKa = 3.72 AGSGPAAGTVTMPLLANCPLSITSPLVSGWDD117 pKa = 3.44 SDD119 pKa = 4.08 PLFWEE124 pKa = 4.43 MWSSVYY130 pKa = 10.66 SHH132 pKa = 5.62 YY133 pKa = 10.18 TVVGVKK139 pKa = 8.25 FTLKK143 pKa = 10.05 TMWAAPTVTPTDD155 pKa = 3.1 TEE157 pKa = 4.32 GAVRR161 pKa = 11.84 VPMRR165 pKa = 11.84 VSLMVNTRR173 pKa = 11.84 EE174 pKa = 4.3 SIPDD178 pKa = 3.46 YY179 pKa = 10.84 VVPEE183 pKa = 3.9 QLQEE187 pKa = 3.62 QGLIHH192 pKa = 6.87 WKK194 pKa = 10.7 DD195 pKa = 3.56 LMTSDD200 pKa = 4.62 LSRR203 pKa = 11.84 VHH205 pKa = 6.22 VVEE208 pKa = 4.5 KK209 pKa = 10.21 FSAKK213 pKa = 10.16 KK214 pKa = 10.22 FFGLSSVSDD223 pKa = 4.13 DD224 pKa = 3.91 PSIGAPCTTYY234 pKa = 10.22 PAALAWFLILVQKK247 pKa = 11.1 VEE249 pKa = 4.15 GTPFGGGGEE258 pKa = 4.21 AGLPSSCSTVPSSFPSFSATRR279 pKa = 11.84 SPRR282 pKa = 11.84 PSRR285 pKa = 11.84 RR286 pKa = 11.84 PRR288 pKa = 11.84 QANKK292 pKa = 10.58 AIILSLFSCCLEE304 pKa = 4.23 KK305 pKa = 10.96 GAVYY309 pKa = 10.67 YY310 pKa = 10.78 GG311 pKa = 3.35
Molecular weight: 34.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.936
IPC2_protein 9.033
IPC_protein 9.077
Toseland 9.458
ProMoST 9.399
Dawson 9.809
Bjellqvist 9.677
Wikipedia 10.014
Rodwell 9.955
Grimsley 9.882
Solomon 9.853
Lehninger 9.809
Nozaki 9.809
DTASelect 9.575
Thurlkill 9.663
EMBOSS 9.94
Sillero 9.823
Patrickios 4.774
IPC_peptide 9.838
IPC2_peptide 8.96
IPC2.peptide.svr19 7.832
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
622
311
311
311.0
35.09
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.592 ± 0.117
3.376 ± 0.117
4.019 ± 0.819
5.305 ± 1.754
3.698 ± 0.117
7.235 ± 0.117
2.572 ± 0.234
3.055 ± 0.351
3.376 ± 0.117
9.486 ± 1.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.572 ± 0.702
3.055 ± 0.351
5.466 ± 0.936
3.698 ± 0.585
8.199 ± 0.351
7.717 ± 2.339
5.788 ± 0.468
8.682 ± 0.702
2.894 ± 0.234
3.215 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here