Amino acid dipepetide frequency for Synechococcus phage S-RIP2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.624AlaAla: 10.624 ± 1.088
0.841AlaCys: 0.841 ± 0.315
6.038AlaAsp: 6.038 ± 0.842
6.114AlaGlu: 6.114 ± 0.999
4.051AlaPhe: 4.051 ± 0.523
5.961AlaGly: 5.961 ± 0.901
0.841AlaHis: 0.841 ± 0.259
4.586AlaIle: 4.586 ± 0.585
3.592AlaLys: 3.592 ± 0.823
6.726AlaLeu: 6.726 ± 0.657
2.064AlaMet: 2.064 ± 0.365
4.051AlaAsn: 4.051 ± 0.444
3.821AlaPro: 3.821 ± 0.518
3.439AlaGln: 3.439 ± 0.538
4.586AlaArg: 4.586 ± 0.831
6.267AlaSer: 6.267 ± 0.828
6.726AlaThr: 6.726 ± 1.26
5.503AlaVal: 5.503 ± 0.682
0.688AlaTrp: 0.688 ± 0.242
3.363AlaTyr: 3.363 ± 0.59
0.0AlaXaa: 0.0 ± 0.0
Cys
0.611CysAla: 0.611 ± 0.235
0.076CysCys: 0.076 ± 0.076
0.229CysAsp: 0.229 ± 0.131
0.535CysGlu: 0.535 ± 0.259
0.153CysPhe: 0.153 ± 0.097
0.306CysGly: 0.306 ± 0.146
0.306CysHis: 0.306 ± 0.154
0.459CysIle: 0.459 ± 0.166
0.688CysLys: 0.688 ± 0.305
0.764CysLeu: 0.764 ± 0.233
0.459CysMet: 0.459 ± 0.198
0.535CysAsn: 0.535 ± 0.206
0.153CysPro: 0.153 ± 0.125
0.306CysGln: 0.306 ± 0.161
0.306CysArg: 0.306 ± 0.171
0.994CysSer: 0.994 ± 0.373
0.764CysThr: 0.764 ± 0.258
0.459CysVal: 0.459 ± 0.221
0.153CysTrp: 0.153 ± 0.118
0.076CysTyr: 0.076 ± 0.066
0.0CysXaa: 0.0 ± 0.0
Asp
6.191AspAla: 6.191 ± 0.764
0.459AspCys: 0.459 ± 0.179
4.051AspAsp: 4.051 ± 0.604
4.356AspGlu: 4.356 ± 0.989
2.064AspPhe: 2.064 ± 0.345
5.197AspGly: 5.197 ± 0.974
0.688AspHis: 0.688 ± 0.18
3.745AspIle: 3.745 ± 0.51
2.828AspLys: 2.828 ± 0.535
5.274AspLeu: 5.274 ± 0.741
1.758AspMet: 1.758 ± 0.408
2.675AspAsn: 2.675 ± 0.595
3.516AspPro: 3.516 ± 0.715
1.376AspGln: 1.376 ± 0.264
2.522AspArg: 2.522 ± 0.403
4.127AspSer: 4.127 ± 0.732
4.127AspThr: 4.127 ± 0.748
3.592AspVal: 3.592 ± 0.411
0.994AspTrp: 0.994 ± 0.262
2.522AspTyr: 2.522 ± 0.552
0.0AspXaa: 0.0 ± 0.0
Glu
5.121GluAla: 5.121 ± 0.711
0.688GluCys: 0.688 ± 0.268
2.751GluAsp: 2.751 ± 0.397
4.204GluGlu: 4.204 ± 0.741
3.363GluPhe: 3.363 ± 0.46
3.669GluGly: 3.669 ± 0.498
0.917GluHis: 0.917 ± 0.294
3.592GluIle: 3.592 ± 0.447
3.286GluLys: 3.286 ± 0.628
5.809GluLeu: 5.809 ± 0.727
1.911GluMet: 1.911 ± 0.404
2.446GluAsn: 2.446 ± 0.431
2.14GluPro: 2.14 ± 0.424
3.516GluGln: 3.516 ± 0.506
2.599GluArg: 2.599 ± 0.466
2.828GluSer: 2.828 ± 0.638
3.898GluThr: 3.898 ± 0.488
4.28GluVal: 4.28 ± 0.667
1.299GluTrp: 1.299 ± 0.302
2.369GluTyr: 2.369 ± 0.488
0.0GluXaa: 0.0 ± 0.0
Phe
1.911PheAla: 1.911 ± 0.286
0.382PheCys: 0.382 ± 0.182
2.828PheAsp: 2.828 ± 0.687
1.452PheGlu: 1.452 ± 0.369
1.911PhePhe: 1.911 ± 0.422
2.293PheGly: 2.293 ± 0.509
0.764PheHis: 0.764 ± 0.302
2.216PheIle: 2.216 ± 0.381
2.064PheLys: 2.064 ± 0.306
2.522PheLeu: 2.522 ± 0.605
1.605PheMet: 1.605 ± 0.357
2.14PheAsn: 2.14 ± 0.446
1.681PhePro: 1.681 ± 0.375
1.758PheGln: 1.758 ± 0.426
1.223PheArg: 1.223 ± 0.313
2.446PheSer: 2.446 ± 0.4
2.828PheThr: 2.828 ± 0.536
2.446PheVal: 2.446 ± 0.374
0.153PheTrp: 0.153 ± 0.104
1.529PheTyr: 1.529 ± 0.494
0.0PheXaa: 0.0 ± 0.0
Gly
6.573GlyAla: 6.573 ± 0.955
0.688GlyCys: 0.688 ± 0.203
4.051GlyAsp: 4.051 ± 0.548
2.751GlyGlu: 2.751 ± 0.369
2.446GlyPhe: 2.446 ± 0.509
7.184GlyGly: 7.184 ± 2.417
0.459GlyHis: 0.459 ± 0.171
3.898GlyIle: 3.898 ± 0.596
4.28GlyLys: 4.28 ± 0.626
4.968GlyLeu: 4.968 ± 0.651
1.529GlyMet: 1.529 ± 0.425
3.669GlyAsn: 3.669 ± 0.571
2.446GlyPro: 2.446 ± 0.412
3.974GlyGln: 3.974 ± 0.858
3.439GlyArg: 3.439 ± 0.503
5.732GlySer: 5.732 ± 1.303
7.261GlyThr: 7.261 ± 1.297
6.114GlyVal: 6.114 ± 0.797
0.764GlyTrp: 0.764 ± 0.224
2.599GlyTyr: 2.599 ± 0.427
0.0GlyXaa: 0.0 ± 0.0
His
0.459HisAla: 0.459 ± 0.203
0.076HisCys: 0.076 ± 0.078
1.299HisAsp: 1.299 ± 0.305
0.841HisGlu: 0.841 ± 0.251
0.611HisPhe: 0.611 ± 0.211
1.07HisGly: 1.07 ± 0.309
0.229HisHis: 0.229 ± 0.133
0.841HisIle: 0.841 ± 0.202
0.917HisLys: 0.917 ± 0.2
1.452HisLeu: 1.452 ± 0.453
0.535HisMet: 0.535 ± 0.195
0.535HisAsn: 0.535 ± 0.205
1.07HisPro: 1.07 ± 0.259
0.764HisGln: 0.764 ± 0.23
0.611HisArg: 0.611 ± 0.205
0.688HisSer: 0.688 ± 0.207
0.917HisThr: 0.917 ± 0.239
0.841HisVal: 0.841 ± 0.221
0.229HisTrp: 0.229 ± 0.167
0.688HisTyr: 0.688 ± 0.21
0.0HisXaa: 0.0 ± 0.0
Ile
5.197IleAla: 5.197 ± 0.635
0.535IleCys: 0.535 ± 0.21
3.516IleAsp: 3.516 ± 0.574
3.286IleGlu: 3.286 ± 0.486
1.758IlePhe: 1.758 ± 0.291
3.669IleGly: 3.669 ± 0.498
0.764IleHis: 0.764 ± 0.316
2.293IleIle: 2.293 ± 0.35
2.904IleLys: 2.904 ± 0.498
3.592IleLeu: 3.592 ± 0.392
1.299IleMet: 1.299 ± 0.305
2.828IleAsn: 2.828 ± 0.455
2.14IlePro: 2.14 ± 0.392
2.369IleGln: 2.369 ± 0.392
2.293IleArg: 2.293 ± 0.444
3.057IleSer: 3.057 ± 0.565
4.127IleThr: 4.127 ± 1.117
2.904IleVal: 2.904 ± 0.459
0.611IleTrp: 0.611 ± 0.186
1.681IleTyr: 1.681 ± 0.351
0.0IleXaa: 0.0 ± 0.0
Lys
3.821LysAla: 3.821 ± 0.838
0.306LysCys: 0.306 ± 0.181
2.522LysAsp: 2.522 ± 0.437
3.363LysGlu: 3.363 ± 0.416
1.299LysPhe: 1.299 ± 0.33
2.599LysGly: 2.599 ± 0.588
0.994LysHis: 0.994 ± 0.347
2.216LysIle: 2.216 ± 0.304
3.592LysLys: 3.592 ± 0.834
5.426LysLeu: 5.426 ± 0.754
1.299LysMet: 1.299 ± 0.437
2.064LysAsn: 2.064 ± 0.318
3.134LysPro: 3.134 ± 0.736
1.605LysGln: 1.605 ± 0.337
2.675LysArg: 2.675 ± 0.512
3.21LysSer: 3.21 ± 0.48
4.204LysThr: 4.204 ± 0.625
3.21LysVal: 3.21 ± 0.621
0.535LysTrp: 0.535 ± 0.165
1.987LysTyr: 1.987 ± 0.378
0.0LysXaa: 0.0 ± 0.0
Leu
6.267LeuAla: 6.267 ± 0.587
0.382LeuCys: 0.382 ± 0.175
4.662LeuAsp: 4.662 ± 0.772
4.204LeuGlu: 4.204 ± 0.627
1.452LeuPhe: 1.452 ± 0.309
5.503LeuGly: 5.503 ± 0.56
1.605LeuHis: 1.605 ± 0.349
4.28LeuIle: 4.28 ± 0.912
3.898LeuLys: 3.898 ± 0.684
6.038LeuLeu: 6.038 ± 0.982
2.522LeuMet: 2.522 ± 0.513
4.968LeuAsn: 4.968 ± 0.637
4.509LeuPro: 4.509 ± 0.657
4.433LeuGln: 4.433 ± 0.911
4.739LeuArg: 4.739 ± 0.628
4.815LeuSer: 4.815 ± 0.533
5.885LeuThr: 5.885 ± 0.555
4.433LeuVal: 4.433 ± 0.556
0.611LeuTrp: 0.611 ± 0.215
2.446LeuTyr: 2.446 ± 0.516
0.0LeuXaa: 0.0 ± 0.0
Met
2.904MetAla: 2.904 ± 0.466
0.306MetCys: 0.306 ± 0.178
1.758MetAsp: 1.758 ± 0.587
1.452MetGlu: 1.452 ± 0.343
1.146MetPhe: 1.146 ± 0.34
1.911MetGly: 1.911 ± 0.538
0.764MetHis: 0.764 ± 0.305
0.841MetIle: 0.841 ± 0.243
1.681MetLys: 1.681 ± 0.47
1.758MetLeu: 1.758 ± 0.329
0.611MetMet: 0.611 ± 0.31
1.299MetAsn: 1.299 ± 0.311
1.146MetPro: 1.146 ± 0.306
1.376MetGln: 1.376 ± 0.455
1.452MetArg: 1.452 ± 0.337
1.987MetSer: 1.987 ± 0.402
2.904MetThr: 2.904 ± 0.491
1.376MetVal: 1.376 ± 0.289
0.076MetTrp: 0.076 ± 0.075
0.535MetTyr: 0.535 ± 0.192
0.0MetXaa: 0.0 ± 0.0
Asn
5.274AsnAla: 5.274 ± 0.615
0.306AsnCys: 0.306 ± 0.132
2.828AsnAsp: 2.828 ± 0.561
3.134AsnGlu: 3.134 ± 0.491
1.834AsnPhe: 1.834 ± 0.389
4.815AsnGly: 4.815 ± 1.263
0.611AsnHis: 0.611 ± 0.196
2.751AsnIle: 2.751 ± 0.574
1.987AsnLys: 1.987 ± 0.402
3.898AsnLeu: 3.898 ± 0.45
1.452AsnMet: 1.452 ± 0.301
2.675AsnAsn: 2.675 ± 0.479
2.675AsnPro: 2.675 ± 0.394
1.911AsnGln: 1.911 ± 0.41
2.751AsnArg: 2.751 ± 0.385
3.439AsnSer: 3.439 ± 0.531
3.669AsnThr: 3.669 ± 0.799
3.592AsnVal: 3.592 ± 0.634
0.382AsnTrp: 0.382 ± 0.168
1.529AsnTyr: 1.529 ± 0.319
0.0AsnXaa: 0.0 ± 0.0
Pro
2.904ProAla: 2.904 ± 0.378
0.153ProCys: 0.153 ± 0.105
2.751ProAsp: 2.751 ± 0.401
3.898ProGlu: 3.898 ± 0.693
1.529ProPhe: 1.529 ± 0.283
3.134ProGly: 3.134 ± 0.562
0.611ProHis: 0.611 ± 0.217
2.293ProIle: 2.293 ± 0.432
2.216ProLys: 2.216 ± 0.492
2.293ProLeu: 2.293 ± 0.408
1.376ProMet: 1.376 ± 0.422
2.751ProAsn: 2.751 ± 0.454
2.828ProPro: 2.828 ± 0.888
2.599ProGln: 2.599 ± 0.625
1.681ProArg: 1.681 ± 0.461
3.592ProSer: 3.592 ± 0.596
3.439ProThr: 3.439 ± 0.713
2.904ProVal: 2.904 ± 0.623
0.994ProTrp: 0.994 ± 0.235
1.452ProTyr: 1.452 ± 0.342
0.0ProXaa: 0.0 ± 0.0
Gln
5.35GlnAla: 5.35 ± 0.685
0.459GlnCys: 0.459 ± 0.174
2.446GlnAsp: 2.446 ± 0.334
2.599GlnGlu: 2.599 ± 0.58
1.681GlnPhe: 1.681 ± 0.376
2.981GlnGly: 2.981 ± 0.499
0.764GlnHis: 0.764 ± 0.216
2.216GlnIle: 2.216 ± 0.337
1.911GlnLys: 1.911 ± 0.389
3.592GlnLeu: 3.592 ± 0.648
1.299GlnMet: 1.299 ± 0.44
1.758GlnAsn: 1.758 ± 0.519
1.529GlnPro: 1.529 ± 0.392
2.904GlnGln: 2.904 ± 0.587
2.828GlnArg: 2.828 ± 0.528
2.522GlnSer: 2.522 ± 0.479
2.904GlnThr: 2.904 ± 0.459
3.21GlnVal: 3.21 ± 0.398
0.306GlnTrp: 0.306 ± 0.124
1.681GlnTyr: 1.681 ± 0.357
0.0GlnXaa: 0.0 ± 0.0
Arg
4.586ArgAla: 4.586 ± 0.539
0.229ArgCys: 0.229 ± 0.13
2.904ArgAsp: 2.904 ± 0.483
2.981ArgGlu: 2.981 ± 0.66
1.529ArgPhe: 1.529 ± 0.265
3.745ArgGly: 3.745 ± 0.608
0.764ArgHis: 0.764 ± 0.286
2.751ArgIle: 2.751 ± 0.425
2.446ArgLys: 2.446 ± 0.531
4.204ArgLeu: 4.204 ± 0.425
1.223ArgMet: 1.223 ± 0.268
2.369ArgAsn: 2.369 ± 0.415
1.987ArgPro: 1.987 ± 0.349
2.293ArgGln: 2.293 ± 0.502
3.134ArgArg: 3.134 ± 0.764
3.439ArgSer: 3.439 ± 0.408
3.057ArgThr: 3.057 ± 0.566
2.446ArgVal: 2.446 ± 0.359
0.688ArgTrp: 0.688 ± 0.249
1.299ArgTyr: 1.299 ± 0.267
0.0ArgXaa: 0.0 ± 0.0
Ser
5.961SerAla: 5.961 ± 0.803
0.535SerCys: 0.535 ± 0.242
4.356SerAsp: 4.356 ± 0.735
3.745SerGlu: 3.745 ± 0.43
2.599SerPhe: 2.599 ± 0.342
6.573SerGly: 6.573 ± 1.329
0.764SerHis: 0.764 ± 0.232
3.134SerIle: 3.134 ± 0.584
2.675SerLys: 2.675 ± 0.622
5.197SerLeu: 5.197 ± 0.623
1.452SerMet: 1.452 ± 0.321
4.356SerAsn: 4.356 ± 0.606
3.057SerPro: 3.057 ± 0.358
2.446SerGln: 2.446 ± 0.49
3.057SerArg: 3.057 ± 0.593
4.815SerSer: 4.815 ± 0.968
5.197SerThr: 5.197 ± 0.997
5.044SerVal: 5.044 ± 0.549
0.917SerTrp: 0.917 ± 0.25
1.911SerTyr: 1.911 ± 0.372
0.0SerXaa: 0.0 ± 0.0
Thr
7.031ThrAla: 7.031 ± 0.926
0.611ThrCys: 0.611 ± 0.279
4.891ThrAsp: 4.891 ± 0.942
4.433ThrGlu: 4.433 ± 0.624
2.216ThrPhe: 2.216 ± 0.46
5.656ThrGly: 5.656 ± 1.107
1.223ThrHis: 1.223 ± 0.367
4.051ThrIle: 4.051 ± 0.796
3.439ThrLys: 3.439 ± 0.419
6.038ThrLeu: 6.038 ± 0.603
1.376ThrMet: 1.376 ± 0.328
4.739ThrAsn: 4.739 ± 0.851
3.286ThrPro: 3.286 ± 0.542
2.981ThrGln: 2.981 ± 0.469
2.675ThrArg: 2.675 ± 0.456
6.191ThrSer: 6.191 ± 0.861
7.108ThrThr: 7.108 ± 1.503
5.809ThrVal: 5.809 ± 0.71
1.299ThrTrp: 1.299 ± 0.359
3.363ThrTyr: 3.363 ± 0.401
0.0ThrXaa: 0.0 ± 0.0
Val
6.573ValAla: 6.573 ± 0.738
0.688ValCys: 0.688 ± 0.264
4.739ValAsp: 4.739 ± 0.61
4.28ValGlu: 4.28 ± 0.815
2.522ValPhe: 2.522 ± 0.455
4.968ValGly: 4.968 ± 0.564
0.917ValHis: 0.917 ± 0.27
2.675ValIle: 2.675 ± 0.492
2.981ValLys: 2.981 ± 0.363
4.127ValLeu: 4.127 ± 0.633
1.911ValMet: 1.911 ± 0.424
3.21ValAsn: 3.21 ± 0.616
2.751ValPro: 2.751 ± 0.677
2.599ValGln: 2.599 ± 0.343
2.828ValArg: 2.828 ± 0.461
5.044ValSer: 5.044 ± 0.703
5.35ValThr: 5.35 ± 1.054
4.433ValVal: 4.433 ± 0.586
0.917ValTrp: 0.917 ± 0.22
1.681ValTyr: 1.681 ± 0.396
0.0ValXaa: 0.0 ± 0.0
Trp
0.611TrpAla: 0.611 ± 0.261
0.076TrpCys: 0.076 ± 0.079
1.07TrpAsp: 1.07 ± 0.292
0.764TrpGlu: 0.764 ± 0.261
0.611TrpPhe: 0.611 ± 0.218
0.306TrpGly: 0.306 ± 0.115
0.229TrpHis: 0.229 ± 0.122
0.688TrpIle: 0.688 ± 0.221
0.535TrpLys: 0.535 ± 0.237
1.529TrpLeu: 1.529 ± 0.433
0.459TrpMet: 0.459 ± 0.193
0.535TrpAsn: 0.535 ± 0.2
0.382TrpPro: 0.382 ± 0.155
0.611TrpGln: 0.611 ± 0.203
0.535TrpArg: 0.535 ± 0.197
0.841TrpSer: 0.841 ± 0.268
1.07TrpThr: 1.07 ± 0.29
0.611TrpVal: 0.611 ± 0.195
0.153TrpTrp: 0.153 ± 0.109
0.382TrpTyr: 0.382 ± 0.182
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.911TyrAla: 1.911 ± 0.314
0.535TyrCys: 0.535 ± 0.25
2.369TyrAsp: 2.369 ± 0.559
2.293TyrGlu: 2.293 ± 0.417
1.376TyrPhe: 1.376 ± 0.371
3.363TyrGly: 3.363 ± 0.6
0.459TyrHis: 0.459 ± 0.164
1.299TyrIle: 1.299 ± 0.249
2.14TyrLys: 2.14 ± 0.535
2.446TyrLeu: 2.446 ± 0.339
1.07TyrMet: 1.07 ± 0.324
1.911TyrAsn: 1.911 ± 0.38
1.146TyrPro: 1.146 ± 0.256
1.681TyrGln: 1.681 ± 0.319
2.14TyrArg: 2.14 ± 0.438
1.758TyrSer: 1.758 ± 0.349
2.981TyrThr: 2.981 ± 0.592
1.987TyrVal: 1.987 ± 0.315
0.229TyrTrp: 0.229 ± 0.138
1.146TyrTyr: 1.146 ± 0.24
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 54 proteins (13085 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski