Belliella baltica (strain DSM 15883 / CIP 108006 / LMG 21964 / BA134)
Average proteome isoelectric point is 6.31
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3609 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I3Z5B7|I3Z5B7_BELBD Uncharacterized protein OS=Belliella baltica (strain DSM 15883 / CIP 108006 / LMG 21964 / BA134) OX=866536 GN=Belba_1846 PE=4 SV=1
MM1 pKa = 7.77 KK2 pKa = 10.56 SRR4 pKa = 11.84 FKK6 pKa = 10.76 SIKK9 pKa = 9.68 FLLIFAGICVLSCIMPLNKK28 pKa = 10.07 SFSQGFNNNEE38 pKa = 4.04 WIFGYY43 pKa = 10.73 CEE45 pKa = 4.96 LDD47 pKa = 3.17 TLKK50 pKa = 10.77 RR51 pKa = 11.84 IVSFGKK57 pKa = 10.52 GEE59 pKa = 3.9 EE60 pKa = 3.96 PVIRR64 pKa = 11.84 EE65 pKa = 3.53 VSGAFNQNNSAVAVDD80 pKa = 4.78 PINGNVVFYY89 pKa = 11.25 SDD91 pKa = 4.14 GEE93 pKa = 4.13 LVYY96 pKa = 10.81 NYY98 pKa = 10.14 NNSPMQGALSINGNSSGRR116 pKa = 11.84 QEE118 pKa = 3.71 IGIGILEE125 pKa = 4.57 FDD127 pKa = 4.01 PNGDD131 pKa = 2.94 KK132 pKa = 10.72 LYY134 pKa = 10.84 YY135 pKa = 9.83 IFYY138 pKa = 8.72 LTTGGQLQYY147 pKa = 11.48 AVVDD151 pKa = 4.01 MNSPGAAVGNEE162 pKa = 3.9 PPQGRR167 pKa = 11.84 VEE169 pKa = 4.1 VFNRR173 pKa = 11.84 SIGPATGSIAVVKK186 pKa = 10.05 SAQSPSYY193 pKa = 10.41 LISFNGGNLISRR205 pKa = 11.84 RR206 pKa = 11.84 IEE208 pKa = 3.71 ATQGEE213 pKa = 4.84 FTEE216 pKa = 4.5 TDD218 pKa = 3.19 DD219 pKa = 4.31 VGIPFTPKK227 pKa = 10.45 AIIFNEE233 pKa = 3.98 ATGQLILIPEE243 pKa = 4.54 NPNEE247 pKa = 4.86 DD248 pKa = 3.18 IVLVDD253 pKa = 4.13 FDD255 pKa = 4.08 TAEE258 pKa = 4.1 GRR260 pKa = 11.84 FGTVNTISQSGGVDD274 pKa = 3.71 SIEE277 pKa = 4.18 GAAFSPDD284 pKa = 2.76 ADD286 pKa = 3.43 YY287 pKa = 10.88 IYY289 pKa = 10.71 FSRR292 pKa = 11.84 GNEE295 pKa = 4.14 LFRR298 pKa = 11.84 VPADD302 pKa = 4.58 DD303 pKa = 5.74 LDD305 pKa = 4.86 ADD307 pKa = 4.18 PEE309 pKa = 4.85 LIPLEE314 pKa = 4.2 NNVFRR319 pKa = 11.84 IYY321 pKa = 10.32 DD322 pKa = 3.54 IKK324 pKa = 11.09 VGPDD328 pKa = 2.64 GRR330 pKa = 11.84 LYY332 pKa = 10.79 YY333 pKa = 9.94 IYY335 pKa = 10.34 EE336 pKa = 4.15 EE337 pKa = 4.53 VAGGPQLIGRR347 pKa = 11.84 VDD349 pKa = 3.59 NPDD352 pKa = 3.55 EE353 pKa = 4.34 EE354 pKa = 4.91 DD355 pKa = 3.97 LEE357 pKa = 4.32 EE358 pKa = 4.82 LEE360 pKa = 4.94 KK361 pKa = 11.47 DD362 pKa = 3.21 EE363 pKa = 6.25 DD364 pKa = 4.13 PFDD367 pKa = 4.57 GVDD370 pKa = 3.7 FCGRR374 pKa = 11.84 IFPQFAPNADD384 pKa = 3.33 VEE386 pKa = 4.52 ATVDD390 pKa = 4.25 FTWEE394 pKa = 3.97 PEE396 pKa = 4.45 EE397 pKa = 4.35 PCSNNPVQLTSVITPEE413 pKa = 3.59 NYY415 pKa = 9.8 RR416 pKa = 11.84 PVSFEE421 pKa = 3.56 WTFEE425 pKa = 4.13 PEE427 pKa = 3.99 LTDD430 pKa = 4.2 EE431 pKa = 5.11 DD432 pKa = 5.26 GEE434 pKa = 4.47 PLDD437 pKa = 3.56 IDD439 pKa = 4.25 YY440 pKa = 10.99 NQEE443 pKa = 3.87 HH444 pKa = 7.09 LLLPAEE450 pKa = 4.44 ATSGEE455 pKa = 4.77 SVTATLTVTFADD467 pKa = 4.45 GTTSTSDD474 pKa = 3.05 PRR476 pKa = 11.84 TINLSEE482 pKa = 4.37 NNLEE486 pKa = 4.72 ANFSPQDD493 pKa = 3.43 TTVCEE498 pKa = 4.3 SCFDD502 pKa = 4.08 LMPLLEE508 pKa = 4.24 VQQGEE513 pKa = 4.68 GGQGGQGPGGPGGPGVGGPGGGNGVYY539 pKa = 10.21 EE540 pKa = 4.37 YY541 pKa = 10.66 FWSNKK546 pKa = 8.34 RR547 pKa = 11.84 DD548 pKa = 3.77 EE549 pKa = 4.64 GWGPEE554 pKa = 4.14 APNQICEE561 pKa = 4.21 PGLYY565 pKa = 8.71 WVLVKK570 pKa = 10.8 EE571 pKa = 4.86 PGSSCYY577 pKa = 10.14 AYY579 pKa = 10.41 ASIRR583 pKa = 11.84 VRR585 pKa = 11.84 MWDD588 pKa = 5.38 LDD590 pKa = 3.92 DD591 pKa = 3.55 QSNNIWYY598 pKa = 9.33 FGDD601 pKa = 3.68 GAGIDD606 pKa = 4.15 FNEE609 pKa = 4.8 DD610 pKa = 3.09 PAGILPTPRR619 pKa = 11.84 PVDD622 pKa = 3.2 PRR624 pKa = 11.84 HH625 pKa = 5.89 PQNIPAGTTTISDD638 pKa = 3.26 EE639 pKa = 4.2 TGQVLFYY646 pKa = 10.75 TDD648 pKa = 4.92 GSTVWDD654 pKa = 4.2 LYY656 pKa = 10.74 GDD658 pKa = 3.86 VMADD662 pKa = 3.45 GEE664 pKa = 4.98 DD665 pKa = 3.22 IGGSNQASEE674 pKa = 4.55 GVIAVAIPQEE684 pKa = 3.96 EE685 pKa = 4.82 TIFYY689 pKa = 10.81 LFTTSTAADD698 pKa = 3.66 GNSQVKK704 pKa = 10.12 FSLVDD709 pKa = 3.59 IKK711 pKa = 11.36 AEE713 pKa = 3.95 NPTGVGNVVSKK724 pKa = 11.44 DD725 pKa = 3.26 NFLFSPATQQSAAIAAGDD743 pKa = 3.89 TTWVLFHH750 pKa = 7.06 EE751 pKa = 5.11 LGNNTFRR758 pKa = 11.84 AYY760 pKa = 9.72 PVGSLGIGPPVFSSVGSTHH779 pKa = 7.13 GYY781 pKa = 7.13 DD782 pKa = 3.11 TGVGSMKK789 pKa = 10.4 FSPDD793 pKa = 2.97 GTKK796 pKa = 10.45 VAVTIQDD803 pKa = 4.02 GACSRR808 pKa = 11.84 VEE810 pKa = 5.24 LFDD813 pKa = 4.78 FDD815 pKa = 3.74 QSTGRR820 pKa = 11.84 LTEE823 pKa = 4.05 YY824 pKa = 11.09 ASVDD828 pKa = 3.98 LDD830 pKa = 4.33 CNDD833 pKa = 3.78 DD834 pKa = 3.63 DD835 pKa = 5.04 VYY837 pKa = 11.54 GVEE840 pKa = 5.07 FSNDD844 pKa = 2.74 SDD846 pKa = 4.54 RR847 pKa = 11.84 IFVTYY852 pKa = 9.85 TGDD855 pKa = 3.35 GGKK858 pKa = 9.59 VEE860 pKa = 4.18 EE861 pKa = 5.68 FIIQSPSSQDD871 pKa = 3.1 EE872 pKa = 4.38 DD873 pKa = 5.61 DD874 pKa = 5.28 DD875 pKa = 5.06 LSCGACFEE883 pKa = 4.57 RR884 pKa = 11.84 ATTSAQRR891 pKa = 11.84 AQCILDD897 pKa = 3.3 SRR899 pKa = 11.84 TDD901 pKa = 3.41 LRR903 pKa = 11.84 INGPIGAIQMGPMGDD918 pKa = 2.92 IYY920 pKa = 10.72 VARR923 pKa = 11.84 PGQNFLGTIQPGGLCEE939 pKa = 4.73 LSTGTTEE946 pKa = 5.45 GFQLLPGTTSNLGLPSFVQQSGSSIPEE973 pKa = 3.86 PAISGPDD980 pKa = 3.6 RR981 pKa = 11.84 LCLDD985 pKa = 4.17 PEE987 pKa = 4.44 NGAEE991 pKa = 4.23 GLFEE995 pKa = 4.43 GAGEE999 pKa = 4.14 PDD1001 pKa = 2.98 IDD1003 pKa = 4.08 SYY1005 pKa = 11.62 FWTIVHH1011 pKa = 6.89 EE1012 pKa = 4.72 DD1013 pKa = 3.97 GEE1015 pKa = 4.76 VIEE1018 pKa = 5.2 SDD1020 pKa = 2.98 LGGPGEE1026 pKa = 4.32 EE1027 pKa = 4.39 NQTLTYY1033 pKa = 10.01 IFQRR1037 pKa = 11.84 EE1038 pKa = 3.89 GLYY1041 pKa = 9.99 TVTLRR1046 pKa = 11.84 VDD1048 pKa = 3.04 RR1049 pKa = 11.84 CGDD1052 pKa = 3.38 PEE1054 pKa = 4.72 HH1055 pKa = 6.74 YY1056 pKa = 10.41 NKK1058 pKa = 10.48 NNSIEE1063 pKa = 4.26 VLVVAPPEE1071 pKa = 3.84 ITLEE1075 pKa = 4.02 NDD1077 pKa = 2.84 VTLCSGTPVTLTAIDD1092 pKa = 4.97 GYY1094 pKa = 11.44 DD1095 pKa = 3.76 PADD1098 pKa = 3.52 GLYY1101 pKa = 10.83 DD1102 pKa = 3.85 FEE1104 pKa = 4.45 WRR1106 pKa = 11.84 NAAGIQFGDD1115 pKa = 3.78 EE1116 pKa = 4.03 NSNEE1120 pKa = 3.86 ITVKK1124 pKa = 10.52 EE1125 pKa = 3.86 EE1126 pKa = 3.72 SIYY1129 pKa = 10.62 TVTVSYY1135 pKa = 11.19 RR1136 pKa = 11.84 NTNQDD1141 pKa = 3.08 EE1142 pKa = 4.69 EE1143 pKa = 5.05 FFDD1146 pKa = 3.95 ACPATRR1152 pKa = 11.84 SVFVGPAFEE1161 pKa = 5.4 FDD1163 pKa = 3.28 LTQTAEE1169 pKa = 4.26 EE1170 pKa = 4.33 VCYY1173 pKa = 11.03 DD1174 pKa = 3.35 EE1175 pKa = 4.53 TLVVFAPDD1183 pKa = 3.78 TPVSGEE1189 pKa = 3.48 WFYY1192 pKa = 11.72 QRR1194 pKa = 11.84 VGDD1197 pKa = 4.07 PNRR1200 pKa = 11.84 VQFPVGLAFEE1210 pKa = 5.29 LEE1212 pKa = 4.14 ITPSIDD1218 pKa = 3.29 LPGPGLYY1225 pKa = 10.08 QIIFVTEE1232 pKa = 3.91 DD1233 pKa = 4.36 PIVEE1237 pKa = 4.27 GCLVEE1242 pKa = 5.19 KK1243 pKa = 10.81 VLEE1246 pKa = 4.27 LEE1248 pKa = 4.5 VFPLPNFEE1256 pKa = 5.47 IVILTNAEE1264 pKa = 4.07 DD1265 pKa = 4.12 CNSPEE1270 pKa = 3.82 GSFEE1274 pKa = 3.93 IEE1276 pKa = 3.64 ALIAMSVLEE1285 pKa = 4.02 IMEE1288 pKa = 4.57 TGDD1291 pKa = 3.17 VFQNVSAGDD1300 pKa = 4.0 FFTLTDD1306 pKa = 4.71 LEE1308 pKa = 4.66 PGNYY1312 pKa = 7.18 TVRR1315 pKa = 11.84 GQTDD1319 pKa = 4.18 FGCEE1323 pKa = 3.92 FTRR1326 pKa = 11.84 TATIQNVNPPQGFEE1340 pKa = 4.4 NITLDD1345 pKa = 4.43 STDD1348 pKa = 3.42 EE1349 pKa = 4.19 SCGVNEE1355 pKa = 5.39 IIPGSITIEE1364 pKa = 3.73 LSAAVQGTYY1373 pKa = 10.3 TITRR1377 pKa = 11.84 QGDD1380 pKa = 3.73 GQEE1383 pKa = 4.05 FTGALDD1389 pKa = 3.72 GQTTVIEE1396 pKa = 4.58 IGHH1399 pKa = 6.84 GDD1401 pKa = 3.66 YY1402 pKa = 10.97 AVQVEE1407 pKa = 4.65 DD1408 pKa = 5.02 ANGCAIPVGDD1418 pKa = 3.54 VTVARR1423 pKa = 11.84 RR1424 pKa = 11.84 FLADD1428 pKa = 4.04 FNVPSNIVACEE1439 pKa = 3.93 SFEE1442 pKa = 5.36 LEE1444 pKa = 4.92 ISTQQNLVFTVTDD1457 pKa = 4.07 PNGDD1461 pKa = 4.77 LINADD1466 pKa = 3.09 ADD1468 pKa = 4.45 GIYY1471 pKa = 10.35 LLNISGVYY1479 pKa = 8.8 TVFGEE1484 pKa = 4.51 DD1485 pKa = 3.95 PDD1487 pKa = 5.29 GVACPRR1493 pKa = 11.84 EE1494 pKa = 3.85 RR1495 pKa = 11.84 TINLTINDD1503 pKa = 5.27 PIQYY1507 pKa = 9.24 EE1508 pKa = 4.24 LSAPQVDD1515 pKa = 4.55 CVDD1518 pKa = 3.59 GVSYY1522 pKa = 10.27 EE1523 pKa = 4.42 AIILDD1528 pKa = 3.92 GTDD1531 pKa = 3.34 PASVFFFWRR1540 pKa = 11.84 NEE1542 pKa = 3.57 SSQVVGRR1549 pKa = 11.84 SQRR1552 pKa = 11.84 FFPSAPGQYY1561 pKa = 9.37 TLDD1564 pKa = 3.44 VQPRR1568 pKa = 11.84 VGSNCPTPPIPFEE1581 pKa = 4.18 VEE1583 pKa = 3.65 EE1584 pKa = 4.15 FAAAVDD1590 pKa = 4.8 FEE1592 pKa = 5.93 LEE1594 pKa = 4.16 VQPFCVGEE1602 pKa = 3.76 PSTFVNLLIDD1612 pKa = 4.74 ADD1614 pKa = 4.19 DD1615 pKa = 3.97 MDD1617 pKa = 4.7 AVGQIRR1623 pKa = 11.84 WFFVQGGNRR1632 pKa = 11.84 TPLPANDD1639 pKa = 4.3 DD1640 pKa = 3.58 ATMVSVDD1647 pKa = 3.46 QANQGTYY1654 pKa = 7.75 QVEE1657 pKa = 4.58 VYY1659 pKa = 11.25 NNFGCLVGRR1668 pKa = 11.84 DD1669 pKa = 3.97 QIQVIQSTLVAPVLQATYY1687 pKa = 10.05 IICALEE1693 pKa = 4.27 NIVFQLDD1700 pKa = 3.03 PGQYY1704 pKa = 10.93 DD1705 pKa = 3.22 NYY1707 pKa = 9.69 EE1708 pKa = 3.96 WYY1710 pKa = 10.9 LEE1712 pKa = 4.52 GEE1714 pKa = 4.68 DD1715 pKa = 4.69 EE1716 pKa = 5.6 PISTDD1721 pKa = 4.51 PIFTPQQEE1729 pKa = 4.33 GNYY1732 pKa = 9.02 RR1733 pKa = 11.84 LVVFDD1738 pKa = 4.51 EE1739 pKa = 4.61 LGCVEE1744 pKa = 5.11 EE1745 pKa = 4.22 ITFEE1749 pKa = 4.17 VVEE1752 pKa = 4.3 DD1753 pKa = 3.76 CEE1755 pKa = 4.62 LRR1757 pKa = 11.84 VRR1759 pKa = 11.84 FPNAMSPSDD1768 pKa = 3.52 ASKK1771 pKa = 11.0 QFLIYY1776 pKa = 10.53 TNDD1779 pKa = 4.02 FIDD1782 pKa = 3.83 EE1783 pKa = 4.33 LSVYY1787 pKa = 10.0 IYY1789 pKa = 10.73 NRR1791 pKa = 11.84 WGEE1794 pKa = 4.18 LIFYY1798 pKa = 9.63 CEE1800 pKa = 3.96 QSNLPGDD1807 pKa = 3.9 AVGGYY1812 pKa = 9.14 CPWDD1816 pKa = 3.36 GTVNGQKK1823 pKa = 10.09 VPIGTYY1829 pKa = 8.39 PVVVRR1834 pKa = 11.84 FRR1836 pKa = 11.84 SNNQNITKK1844 pKa = 8.0 TLRR1847 pKa = 11.84 EE1848 pKa = 4.4 AIVVIEE1854 pKa = 3.99
Molecular weight: 202.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.72
IPC2_protein 3.757
IPC_protein 3.783
Toseland 3.579
ProMoST 3.923
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.656
Rodwell 3.605
Grimsley 3.478
Solomon 3.757
Lehninger 3.706
Nozaki 3.859
DTASelect 4.062
Thurlkill 3.605
EMBOSS 3.668
Sillero 3.897
Patrickios 0.858
IPC_peptide 3.757
IPC2_peptide 3.884
IPC2.peptide.svr19 3.802
Protein with the highest isoelectric point:
>tr|I3Z591|I3Z591_BELBD PIF1 helicase/Helicase OS=Belliella baltica (strain DSM 15883 / CIP 108006 / LMG 21964 / BA134) OX=866536 GN=Belba_1817 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.56 RR3 pKa = 11.84 TFQPSRR9 pKa = 11.84 RR10 pKa = 11.84 KK11 pKa = 9.62 RR12 pKa = 11.84 RR13 pKa = 11.84 NKK15 pKa = 9.5 HH16 pKa = 3.99 GFRR19 pKa = 11.84 EE20 pKa = 4.18 RR21 pKa = 11.84 MSTANGRR28 pKa = 11.84 RR29 pKa = 11.84 VIRR32 pKa = 11.84 SRR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.0 GRR39 pKa = 11.84 HH40 pKa = 5.41 KK41 pKa = 10.43 LTVSSEE47 pKa = 4.01 KK48 pKa = 9.88 TLKK51 pKa = 10.51 KK52 pKa = 10.72
Molecular weight: 6.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.368
IPC2_protein 10.789
IPC_protein 12.369
Toseland 12.53
ProMoST 13.027
Dawson 12.53
Bjellqvist 12.53
Wikipedia 13.013
Rodwell 12.281
Grimsley 12.574
Solomon 13.027
Lehninger 12.925
Nozaki 12.53
DTASelect 12.53
Thurlkill 12.53
EMBOSS 13.027
Sillero 12.53
Patrickios 12.003
IPC_peptide 13.027
IPC2_peptide 12.018
IPC2.peptide.svr19 9.088
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3609
0
3609
1180055
29
3807
327.0
36.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.334 ± 0.039
0.678 ± 0.013
5.461 ± 0.031
7.187 ± 0.039
5.459 ± 0.034
6.636 ± 0.04
1.779 ± 0.025
7.868 ± 0.038
7.427 ± 0.05
9.706 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.405 ± 0.019
5.482 ± 0.035
3.594 ± 0.024
3.621 ± 0.02
3.899 ± 0.03
6.564 ± 0.029
4.951 ± 0.039
6.049 ± 0.04
1.12 ± 0.015
3.782 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here