Propionibacterium phage P1.1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Pahexavirus; Propionibacterium virus P1.1

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 45 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K4HNN5|K4HNN5_9CAUD Helix-turn-helix DNA-binding protein OS=Propionibacterium phage P1.1 OX=1229792 GN=27 PE=4 SV=1
MM1 pKa = 7.49NNTHH5 pKa = 6.9NITYY9 pKa = 7.31TTLNTAIHH17 pKa = 7.0RR18 pKa = 11.84IVQQQPTNMQQLEE31 pKa = 4.44NIVDD35 pKa = 4.0SVEE38 pKa = 3.94NQYY41 pKa = 10.93GVPISLDD48 pKa = 3.46NVNLTVNEE56 pKa = 3.98VSLDD60 pKa = 3.74DD61 pKa = 4.84LAIDD65 pKa = 4.03QDD67 pKa = 4.19TLDD70 pKa = 4.0EE71 pKa = 4.48CSEE74 pKa = 4.13ILWLCDD80 pKa = 3.46STGHH84 pKa = 6.22PTNNSNTRR92 pKa = 11.84DD93 pKa = 3.59NSEE96 pKa = 4.05DD97 pKa = 3.4QSPYY101 pKa = 11.08ASQEE105 pKa = 3.89ALDD108 pKa = 3.92WLAGIAYY115 pKa = 8.03QAKK118 pKa = 9.8LLQAAADD125 pKa = 4.37EE126 pKa = 4.43IRR128 pKa = 11.84WAIIRR133 pKa = 11.84HH134 pKa = 5.92CDD136 pKa = 2.84NHH138 pKa = 7.05KK139 pKa = 10.93NVIGRR144 pKa = 11.84NVLDD148 pKa = 3.77QASDD152 pKa = 3.81TISTCLHH159 pKa = 7.12LYY161 pKa = 10.6QMLEE165 pKa = 3.9DD166 pKa = 4.09TIDD169 pKa = 3.64RR170 pKa = 11.84NEE172 pKa = 3.83SS173 pKa = 3.04

Molecular weight: 19.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K4HNY2|K4HNY2_9CAUD Scaffolding protein OS=Propionibacterium phage P1.1 OX=1229792 GN=5 PE=4 SV=1
MM1 pKa = 7.15MAHH4 pKa = 6.39TNRR7 pKa = 11.84TASASHH13 pKa = 5.86RR14 pKa = 11.84RR15 pKa = 11.84WRR17 pKa = 11.84QRR19 pKa = 11.84LITQARR25 pKa = 11.84QQGQTEE31 pKa = 4.38CPLCGATITWNTHH44 pKa = 5.99DD45 pKa = 5.37LPTSPEE51 pKa = 3.95ADD53 pKa = 3.64HH54 pKa = 6.3ITPVSRR60 pKa = 11.84GGLNTLDD67 pKa = 3.74NGQIICRR74 pKa = 11.84TCNRR78 pKa = 11.84SKK80 pKa = 11.33GNRR83 pKa = 11.84SEE85 pKa = 4.25PNIKK89 pKa = 9.81FQQQTTKK96 pKa = 10.53TLIPWW101 pKa = 3.75

Molecular weight: 11.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

45

0

45

8974

27

921

199.4

21.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.94 ± 0.615

1.125 ± 0.18

6.53 ± 0.495

4.769 ± 0.44

2.808 ± 0.224

8.569 ± 0.56

2.574 ± 0.333

4.925 ± 0.395

4.056 ± 0.287

7.433 ± 0.261

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.596 ± 0.301

3.778 ± 0.323

5.126 ± 0.323

4.435 ± 0.301

6.04 ± 0.394

6.619 ± 0.283

6.162 ± 0.436

7.778 ± 0.619

2.017 ± 0.214

2.719 ± 0.244
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski