Amino acid dipepetide frequency for Pseudomonas sp. HPB0071

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.156AlaAla: 11.156 ± 0.103
1.193AlaCys: 1.193 ± 0.028
5.275AlaAsp: 5.275 ± 0.055
6.563AlaGlu: 6.563 ± 0.066
3.757AlaPhe: 3.757 ± 0.053
7.932AlaGly: 7.932 ± 0.081
2.209AlaHis: 2.209 ± 0.035
5.416AlaIle: 5.416 ± 0.061
3.718AlaLys: 3.718 ± 0.055
12.948AlaLeu: 12.948 ± 0.12
2.854AlaMet: 2.854 ± 0.044
3.045AlaAsn: 3.045 ± 0.053
4.161AlaPro: 4.161 ± 0.053
4.758AlaGln: 4.758 ± 0.064
6.648AlaArg: 6.648 ± 0.064
6.142AlaSer: 6.142 ± 0.068
4.867AlaThr: 4.867 ± 0.054
7.127AlaVal: 7.127 ± 0.074
1.476AlaTrp: 1.476 ± 0.036
2.64AlaTyr: 2.64 ± 0.041
0.0AlaXaa: 0.0 ± 0.0
Cys
0.93CysAla: 0.93 ± 0.024
0.144CysCys: 0.144 ± 0.009
0.553CysAsp: 0.553 ± 0.019
0.573CysGlu: 0.573 ± 0.019
0.367CysPhe: 0.367 ± 0.015
0.912CysGly: 0.912 ± 0.028
0.285CysHis: 0.285 ± 0.012
0.505CysIle: 0.505 ± 0.019
0.3CysLys: 0.3 ± 0.012
1.185CysLeu: 1.185 ± 0.028
0.243CysMet: 0.243 ± 0.011
0.273CysAsn: 0.273 ± 0.013
0.465CysPro: 0.465 ± 0.018
0.388CysGln: 0.388 ± 0.016
0.617CysArg: 0.617 ± 0.021
0.679CysSer: 0.679 ± 0.02
0.48CysThr: 0.48 ± 0.017
0.668CysVal: 0.668 ± 0.022
0.161CysTrp: 0.161 ± 0.01
0.282CysTyr: 0.282 ± 0.012
0.0CysXaa: 0.0 ± 0.0
Asp
5.169AspAla: 5.169 ± 0.055
0.476AspCys: 0.476 ± 0.016
2.752AspAsp: 2.752 ± 0.04
3.727AspGlu: 3.727 ± 0.054
2.023AspPhe: 2.023 ± 0.041
3.797AspGly: 3.797 ± 0.06
1.166AspHis: 1.166 ± 0.029
2.947AspIle: 2.947 ± 0.046
2.014AspLys: 2.014 ± 0.037
5.786AspLeu: 5.786 ± 0.055
1.274AspMet: 1.274 ± 0.03
1.588AspAsn: 1.588 ± 0.037
2.81AspPro: 2.81 ± 0.044
2.276AspGln: 2.276 ± 0.038
3.278AspArg: 3.278 ± 0.041
2.776AspSer: 2.776 ± 0.041
2.635AspThr: 2.635 ± 0.041
3.647AspVal: 3.647 ± 0.051
0.877AspTrp: 0.877 ± 0.022
1.685AspTyr: 1.685 ± 0.033
0.0AspXaa: 0.0 ± 0.0
Glu
6.88GluAla: 6.88 ± 0.086
0.479GluCys: 0.479 ± 0.017
2.642GluAsp: 2.642 ± 0.044
3.604GluGlu: 3.604 ± 0.06
1.781GluPhe: 1.781 ± 0.041
4.229GluGly: 4.229 ± 0.051
1.752GluHis: 1.752 ± 0.03
2.995GluIle: 2.995 ± 0.046
2.477GluLys: 2.477 ± 0.042
6.784GluLeu: 6.784 ± 0.075
1.411GluMet: 1.411 ± 0.029
1.781GluAsn: 1.781 ± 0.033
2.679GluPro: 2.679 ± 0.052
3.619GluGln: 3.619 ± 0.05
5.031GluArg: 5.031 ± 0.057
3.193GluSer: 3.193 ± 0.044
3.14GluThr: 3.14 ± 0.046
4.002GluVal: 4.002 ± 0.056
0.836GluTrp: 0.836 ± 0.022
1.303GluTyr: 1.303 ± 0.031
0.0GluXaa: 0.0 ± 0.0
Phe
3.621PheAla: 3.621 ± 0.049
0.452PheCys: 0.452 ± 0.016
2.306PheAsp: 2.306 ± 0.036
2.176PheGlu: 2.176 ± 0.037
1.497PhePhe: 1.497 ± 0.03
3.115PheGly: 3.115 ± 0.048
0.769PheHis: 0.769 ± 0.023
2.041PheIle: 2.041 ± 0.038
1.429PheLys: 1.429 ± 0.032
3.42PheLeu: 3.42 ± 0.052
0.887PheMet: 0.887 ± 0.022
1.374PheAsn: 1.374 ± 0.031
1.468PhePro: 1.468 ± 0.029
1.201PheGln: 1.201 ± 0.027
1.926PheArg: 1.926 ± 0.034
2.591PheSer: 2.591 ± 0.05
2.095PheThr: 2.095 ± 0.036
2.428PheVal: 2.428 ± 0.039
0.583PheTrp: 0.583 ± 0.022
1.108PheTyr: 1.108 ± 0.027
0.0PheXaa: 0.0 ± 0.0
Gly
6.595GlyAla: 6.595 ± 0.07
0.916GlyCys: 0.916 ± 0.026
3.645GlyAsp: 3.645 ± 0.04
4.436GlyGlu: 4.436 ± 0.063
3.312GlyPhe: 3.312 ± 0.045
5.442GlyGly: 5.442 ± 0.084
1.85GlyHis: 1.85 ± 0.031
4.55GlyIle: 4.55 ± 0.053
3.464GlyLys: 3.464 ± 0.05
8.658GlyLeu: 8.658 ± 0.09
2.157GlyMet: 2.157 ± 0.038
2.453GlyAsn: 2.453 ± 0.052
2.468GlyPro: 2.468 ± 0.037
3.406GlyGln: 3.406 ± 0.049
4.664GlyArg: 4.664 ± 0.052
4.583GlySer: 4.583 ± 0.058
3.892GlyThr: 3.892 ± 0.053
5.507GlyVal: 5.507 ± 0.066
1.342GlyTrp: 1.342 ± 0.031
2.567GlyTyr: 2.567 ± 0.043
0.0GlyXaa: 0.0 ± 0.0
His
2.194HisAla: 2.194 ± 0.033
0.303HisCys: 0.303 ± 0.015
1.284HisAsp: 1.284 ± 0.031
1.409HisGlu: 1.409 ± 0.037
1.008HisPhe: 1.008 ± 0.027
1.872HisGly: 1.872 ± 0.039
0.647HisHis: 0.647 ± 0.023
1.125HisIle: 1.125 ± 0.026
0.715HisLys: 0.715 ± 0.021
2.521HisLeu: 2.521 ± 0.039
0.582HisMet: 0.582 ± 0.019
0.639HisAsn: 0.639 ± 0.018
1.404HisPro: 1.404 ± 0.032
0.95HisGln: 0.95 ± 0.024
1.429HisArg: 1.429 ± 0.031
1.248HisSer: 1.248 ± 0.029
1.126HisThr: 1.126 ± 0.026
1.526HisVal: 1.526 ± 0.033
0.423HisTrp: 0.423 ± 0.018
0.839HisTyr: 0.839 ± 0.024
0.0HisXaa: 0.0 ± 0.0
Ile
5.948IleAla: 5.948 ± 0.066
0.543IleCys: 0.543 ± 0.02
3.387IleAsp: 3.387 ± 0.047
3.763IleGlu: 3.763 ± 0.058
1.562IlePhe: 1.562 ± 0.029
4.509IleGly: 4.509 ± 0.056
1.159IleHis: 1.159 ± 0.026
2.414IleIle: 2.414 ± 0.045
2.016IleLys: 2.016 ± 0.042
4.515IleLeu: 4.515 ± 0.053
1.028IleMet: 1.028 ± 0.023
1.973IleAsn: 1.973 ± 0.036
2.477IlePro: 2.477 ± 0.036
1.96IleGln: 1.96 ± 0.036
3.257IleArg: 3.257 ± 0.041
3.222IleSer: 3.222 ± 0.036
2.963IleThr: 2.963 ± 0.04
3.59IleVal: 3.59 ± 0.053
0.591IleTrp: 0.591 ± 0.02
1.184IleTyr: 1.184 ± 0.028
0.0IleXaa: 0.0 ± 0.0
Lys
4.584LysAla: 4.584 ± 0.065
0.209LysCys: 0.209 ± 0.013
1.97LysAsp: 1.97 ± 0.034
2.034LysGlu: 2.034 ± 0.037
0.855LysPhe: 0.855 ± 0.027
2.931LysGly: 2.931 ± 0.044
0.867LysHis: 0.867 ± 0.022
1.629LysIle: 1.629 ± 0.033
1.543LysLys: 1.543 ± 0.045
3.751LysLeu: 3.751 ± 0.047
0.756LysMet: 0.756 ± 0.024
1.126LysAsn: 1.126 ± 0.028
2.357LysPro: 2.357 ± 0.036
1.762LysGln: 1.762 ± 0.039
2.731LysArg: 2.731 ± 0.045
2.035LysSer: 2.035 ± 0.037
2.125LysThr: 2.125 ± 0.043
2.793LysVal: 2.793 ± 0.045
0.407LysTrp: 0.407 ± 0.017
0.773LysTyr: 0.773 ± 0.023
0.0LysXaa: 0.0 ± 0.0
Leu
12.774LeuAla: 12.774 ± 0.109
1.231LeuCys: 1.231 ± 0.028
6.375LeuAsp: 6.375 ± 0.062
6.769LeuGlu: 6.769 ± 0.075
4.15LeuPhe: 4.15 ± 0.06
8.592LeuGly: 8.592 ± 0.094
2.363LeuHis: 2.363 ± 0.041
6.068LeuIle: 6.068 ± 0.062
4.679LeuLys: 4.679 ± 0.054
12.169LeuLeu: 12.169 ± 0.134
2.707LeuMet: 2.707 ± 0.039
3.781LeuAsn: 3.781 ± 0.054
5.962LeuPro: 5.962 ± 0.074
3.918LeuGln: 3.918 ± 0.05
6.677LeuArg: 6.677 ± 0.081
7.606LeuSer: 7.606 ± 0.077
6.045LeuThr: 6.045 ± 0.07
7.478LeuVal: 7.478 ± 0.068
1.344LeuTrp: 1.344 ± 0.031
2.672LeuTyr: 2.672 ± 0.045
0.0LeuXaa: 0.0 ± 0.0
Met
2.73MetAla: 2.73 ± 0.043
0.168MetCys: 0.168 ± 0.009
1.201MetAsp: 1.201 ± 0.03
1.077MetGlu: 1.077 ± 0.026
0.745MetPhe: 0.745 ± 0.022
1.722MetGly: 1.722 ± 0.034
0.534MetHis: 0.534 ± 0.018
1.227MetIle: 1.227 ± 0.027
0.982MetLys: 0.982 ± 0.025
2.675MetLeu: 2.675 ± 0.046
0.563MetMet: 0.563 ± 0.019
0.901MetAsn: 0.901 ± 0.023
1.368MetPro: 1.368 ± 0.028
1.115MetGln: 1.115 ± 0.028
1.553MetArg: 1.553 ± 0.034
1.785MetSer: 1.785 ± 0.032
1.707MetThr: 1.707 ± 0.031
1.571MetVal: 1.571 ± 0.03
0.178MetTrp: 0.178 ± 0.01
0.408MetTyr: 0.408 ± 0.016
0.0MetXaa: 0.0 ± 0.0
Asn
3.281AsnAla: 3.281 ± 0.048
0.279AsnCys: 0.279 ± 0.014
1.693AsnAsp: 1.693 ± 0.039
1.819AsnGlu: 1.819 ± 0.035
1.069AsnPhe: 1.069 ± 0.027
2.712AsnGly: 2.712 ± 0.045
0.704AsnHis: 0.704 ± 0.023
1.554AsnIle: 1.554 ± 0.034
1.07AsnLys: 1.07 ± 0.028
3.339AsnLeu: 3.339 ± 0.051
0.645AsnMet: 0.645 ± 0.021
1.127AsnAsn: 1.127 ± 0.037
1.993AsnPro: 1.993 ± 0.037
1.411AsnGln: 1.411 ± 0.029
2.055AsnArg: 2.055 ± 0.035
1.669AsnSer: 1.669 ± 0.037
1.71AsnThr: 1.71 ± 0.041
2.126AsnVal: 2.126 ± 0.037
0.5AsnTrp: 0.5 ± 0.017
0.854AsnTyr: 0.854 ± 0.028
0.0AsnXaa: 0.0 ± 0.0
Pro
5.043ProAla: 5.043 ± 0.059
0.372ProCys: 0.372 ± 0.016
2.998ProAsp: 2.998 ± 0.047
3.616ProGlu: 3.616 ± 0.058
1.856ProPhe: 1.856 ± 0.033
3.619ProGly: 3.619 ± 0.052
1.081ProHis: 1.081 ± 0.026
2.244ProIle: 2.244 ± 0.039
1.72ProLys: 1.72 ± 0.032
5.358ProLeu: 5.358 ± 0.058
1.101ProMet: 1.101 ± 0.027
1.476ProAsn: 1.476 ± 0.032
1.887ProPro: 1.887 ± 0.046
1.875ProGln: 1.875 ± 0.034
2.518ProArg: 2.518 ± 0.042
2.818ProSer: 2.818 ± 0.042
2.337ProThr: 2.337 ± 0.034
3.874ProVal: 3.874 ± 0.052
0.687ProTrp: 0.687 ± 0.021
1.349ProTyr: 1.349 ± 0.029
0.0ProXaa: 0.0 ± 0.0
Gln
5.389GlnAla: 5.389 ± 0.063
0.335GlnCys: 0.335 ± 0.014
1.893GlnAsp: 1.893 ± 0.035
2.396GlnGlu: 2.396 ± 0.043
1.285GlnPhe: 1.285 ± 0.031
3.271GlnGly: 3.271 ± 0.044
1.093GlnHis: 1.093 ± 0.03
2.14GlnIle: 2.14 ± 0.032
1.491GlnLys: 1.491 ± 0.031
4.848GlnLeu: 4.848 ± 0.069
1.036GlnMet: 1.036 ± 0.026
1.277GlnAsn: 1.277 ± 0.032
2.268GlnPro: 2.268 ± 0.043
2.493GlnGln: 2.493 ± 0.047
3.253GlnArg: 3.253 ± 0.05
2.343GlnSer: 2.343 ± 0.043
2.269GlnThr: 2.269 ± 0.043
3.206GlnVal: 3.206 ± 0.047
0.673GlnTrp: 0.673 ± 0.02
1.033GlnTyr: 1.033 ± 0.026
0.0GlnXaa: 0.0 ± 0.0
Arg
5.563ArgAla: 5.563 ± 0.061
0.605ArgCys: 0.605 ± 0.018
3.291ArgAsp: 3.291 ± 0.044
4.156ArgGlu: 4.156 ± 0.061
2.891ArgPhe: 2.891 ± 0.042
3.73ArgGly: 3.73 ± 0.049
1.727ArgHis: 1.727 ± 0.036
3.691ArgIle: 3.691 ± 0.054
2.281ArgLys: 2.281 ± 0.037
8.227ArgLeu: 8.227 ± 0.088
1.651ArgMet: 1.651 ± 0.036
2.092ArgAsn: 2.092 ± 0.035
2.716ArgPro: 2.716 ± 0.041
3.34ArgGln: 3.34 ± 0.057
4.322ArgArg: 4.322 ± 0.059
3.495ArgSer: 3.495 ± 0.047
3.026ArgThr: 3.026 ± 0.044
4.153ArgVal: 4.153 ± 0.056
1.084ArgTrp: 1.084 ± 0.025
2.195ArgTyr: 2.195 ± 0.038
0.0ArgXaa: 0.0 ± 0.0
Ser
5.964SerAla: 5.964 ± 0.073
0.54SerCys: 0.54 ± 0.017
2.983SerAsp: 2.983 ± 0.04
3.376SerGlu: 3.376 ± 0.047
2.276SerPhe: 2.276 ± 0.037
5.06SerGly: 5.06 ± 0.055
1.41SerHis: 1.41 ± 0.027
3.095SerIle: 3.095 ± 0.045
2.001SerLys: 2.001 ± 0.033
7.169SerLeu: 7.169 ± 0.078
1.537SerMet: 1.537 ± 0.031
1.837SerAsn: 1.837 ± 0.039
2.83SerPro: 2.83 ± 0.042
2.562SerGln: 2.562 ± 0.047
3.83SerArg: 3.83 ± 0.051
3.842SerSer: 3.842 ± 0.062
3.2SerThr: 3.2 ± 0.051
4.131SerVal: 4.131 ± 0.056
0.833SerTrp: 0.833 ± 0.026
1.554SerTyr: 1.554 ± 0.034
0.0SerXaa: 0.0 ± 0.0
Thr
5.304ThrAla: 5.304 ± 0.063
0.496ThrCys: 0.496 ± 0.018
2.573ThrAsp: 2.573 ± 0.042
2.674ThrGlu: 2.674 ± 0.039
1.918ThrPhe: 1.918 ± 0.033
4.487ThrGly: 4.487 ± 0.057
1.261ThrHis: 1.261 ± 0.028
2.407ThrIle: 2.407 ± 0.036
1.405ThrLys: 1.405 ± 0.032
7.021ThrLeu: 7.021 ± 0.068
0.964ThrMet: 0.964 ± 0.027
1.406ThrAsn: 1.406 ± 0.031
3.207ThrPro: 3.207 ± 0.048
2.306ThrGln: 2.306 ± 0.042
3.239ThrArg: 3.239 ± 0.046
2.968ThrSer: 2.968 ± 0.046
2.65ThrThr: 2.65 ± 0.043
3.815ThrVal: 3.815 ± 0.052
0.707ThrTrp: 0.707 ± 0.021
1.433ThrTyr: 1.433 ± 0.032
0.0ThrXaa: 0.0 ± 0.0
Val
7.058ValAla: 7.058 ± 0.073
0.772ValCys: 0.772 ± 0.023
3.821ValAsp: 3.821 ± 0.051
4.34ValGlu: 4.34 ± 0.059
2.511ValPhe: 2.511 ± 0.045
4.948ValGly: 4.948 ± 0.062
1.366ValHis: 1.366 ± 0.028
4.13ValIle: 4.13 ± 0.056
2.48ValLys: 2.48 ± 0.041
7.801ValLeu: 7.801 ± 0.08
1.878ValMet: 1.878 ± 0.033
2.206ValAsn: 2.206 ± 0.04
3.266ValPro: 3.266 ± 0.05
2.524ValGln: 2.524 ± 0.041
4.175ValArg: 4.175 ± 0.045
4.483ValSer: 4.483 ± 0.053
3.938ValThr: 3.938 ± 0.049
5.062ValVal: 5.062 ± 0.064
0.89ValTrp: 0.89 ± 0.027
1.731ValTyr: 1.731 ± 0.034
0.0ValXaa: 0.0 ± 0.0
Trp
1.15TrpAla: 1.15 ± 0.03
0.157TrpCys: 0.157 ± 0.01
0.626TrpAsp: 0.626 ± 0.02
0.651TrpGlu: 0.651 ± 0.022
0.557TrpPhe: 0.557 ± 0.019
0.876TrpGly: 0.876 ± 0.023
0.399TrpHis: 0.399 ± 0.017
0.746TrpIle: 0.746 ± 0.022
0.534TrpLys: 0.534 ± 0.019
2.118TrpLeu: 2.118 ± 0.046
0.392TrpMet: 0.392 ± 0.015
0.46TrpAsn: 0.46 ± 0.017
0.669TrpPro: 0.669 ± 0.021
0.832TrpGln: 0.832 ± 0.024
1.017TrpArg: 1.017 ± 0.026
0.891TrpSer: 0.891 ± 0.023
0.66TrpThr: 0.66 ± 0.019
0.957TrpVal: 0.957 ± 0.026
0.233TrpTrp: 0.233 ± 0.014
0.329TrpTyr: 0.329 ± 0.014
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.551TyrAla: 2.551 ± 0.041
0.337TyrCys: 0.337 ± 0.014
1.437TyrAsp: 1.437 ± 0.03
1.459TyrGlu: 1.459 ± 0.027
1.068TyrPhe: 1.068 ± 0.026
2.153TyrGly: 2.153 ± 0.038
0.601TyrHis: 0.601 ± 0.02
1.137TyrIle: 1.137 ± 0.03
0.919TyrLys: 0.919 ± 0.027
3.068TyrLeu: 3.068 ± 0.043
0.542TyrMet: 0.542 ± 0.018
0.794TyrAsn: 0.794 ± 0.021
1.373TyrPro: 1.373 ± 0.026
1.296TyrGln: 1.296 ± 0.031
2.017TyrArg: 2.017 ± 0.041
1.662TyrSer: 1.662 ± 0.035
1.371TyrThr: 1.371 ± 0.029
1.721TyrVal: 1.721 ± 0.035
0.432TyrTrp: 0.432 ± 0.016
0.76TyrTyr: 0.76 ± 0.022
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5313 proteins (1648862 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski