Amino acid dipepetide frequency for Methylobacterium sp. Leaf99

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
21.751AlaAla: 21.751 ± 0.221
1.261AlaCys: 1.261 ± 0.031
7.638AlaAsp: 7.638 ± 0.082
8.503AlaGlu: 8.503 ± 0.104
4.813AlaPhe: 4.813 ± 0.072
13.015AlaGly: 13.015 ± 0.127
2.519AlaHis: 2.519 ± 0.053
6.156AlaIle: 6.156 ± 0.083
3.821AlaLys: 3.821 ± 0.075
15.858AlaLeu: 15.858 ± 0.145
3.486AlaMet: 3.486 ± 0.051
2.627AlaAsn: 2.627 ± 0.056
7.494AlaPro: 7.494 ± 0.098
4.072AlaGln: 4.072 ± 0.056
11.778AlaArg: 11.778 ± 0.129
6.356AlaSer: 6.356 ± 0.073
7.245AlaThr: 7.245 ± 0.082
10.632AlaVal: 10.632 ± 0.11
1.832AlaTrp: 1.832 ± 0.041
2.894AlaTyr: 2.894 ± 0.052
0.0AlaXaa: 0.0 ± 0.0
Cys
1.029CysAla: 1.029 ± 0.031
0.072CysCys: 0.072 ± 0.008
0.488CysAsp: 0.488 ± 0.02
0.391CysGlu: 0.391 ± 0.019
0.256CysPhe: 0.256 ± 0.014
0.864CysGly: 0.864 ± 0.031
0.184CysHis: 0.184 ± 0.012
0.302CysIle: 0.302 ± 0.016
0.133CysLys: 0.133 ± 0.011
0.901CysLeu: 0.901 ± 0.031
0.137CysMet: 0.137 ± 0.01
0.161CysAsn: 0.161 ± 0.013
0.446CysPro: 0.446 ± 0.019
0.168CysGln: 0.168 ± 0.012
0.662CysArg: 0.662 ± 0.024
0.317CysSer: 0.317 ± 0.017
0.413CysThr: 0.413 ± 0.021
0.594CysVal: 0.594 ± 0.022
0.091CysTrp: 0.091 ± 0.009
0.131CysTyr: 0.131 ± 0.01
0.0CysXaa: 0.0 ± 0.0
Asp
8.23AspAla: 8.23 ± 0.105
0.399AspCys: 0.399 ± 0.021
2.934AspAsp: 2.934 ± 0.054
3.052AspGlu: 3.052 ± 0.058
1.871AspPhe: 1.871 ± 0.04
6.099AspGly: 6.099 ± 0.085
1.242AspHis: 1.242 ± 0.034
2.325AspIle: 2.325 ± 0.046
1.306AspLys: 1.306 ± 0.039
6.786AspLeu: 6.786 ± 0.084
0.961AspMet: 0.961 ± 0.027
0.913AspAsn: 0.913 ± 0.031
4.086AspPro: 4.086 ± 0.057
1.283AspGln: 1.283 ± 0.033
4.937AspArg: 4.937 ± 0.075
1.89AspSer: 1.89 ± 0.038
2.804AspThr: 2.804 ± 0.051
4.166AspVal: 4.166 ± 0.064
0.88AspTrp: 0.88 ± 0.027
1.25AspTyr: 1.25 ± 0.032
0.0AspXaa: 0.0 ± 0.0
Glu
9.086GluAla: 9.086 ± 0.109
0.307GluCys: 0.307 ± 0.016
2.508GluAsp: 2.508 ± 0.053
2.393GluGlu: 2.393 ± 0.05
1.378GluPhe: 1.378 ± 0.037
4.477GluGly: 4.477 ± 0.066
1.069GluHis: 1.069 ± 0.032
3.05GluIle: 3.05 ± 0.06
1.479GluLys: 1.479 ± 0.037
3.998GluLeu: 3.998 ± 0.061
1.138GluMet: 1.138 ± 0.031
1.141GluAsn: 1.141 ± 0.032
3.006GluPro: 3.006 ± 0.061
1.396GluGln: 1.396 ± 0.038
5.281GluArg: 5.281 ± 0.08
2.208GluSer: 2.208 ± 0.042
3.83GluThr: 3.83 ± 0.056
3.57GluVal: 3.57 ± 0.051
0.505GluTrp: 0.505 ± 0.021
0.728GluTyr: 0.728 ± 0.023
0.0GluXaa: 0.0 ± 0.0
Phe
4.761PheAla: 4.761 ± 0.063
0.323PheCys: 0.323 ± 0.017
2.355PheAsp: 2.355 ± 0.043
1.869PheGlu: 1.869 ± 0.043
1.168PhePhe: 1.168 ± 0.035
3.566PheGly: 3.566 ± 0.062
0.656PheHis: 0.656 ± 0.026
1.115PheIle: 1.115 ± 0.037
0.798PheLys: 0.798 ± 0.027
3.237PheLeu: 3.237 ± 0.054
0.594PheMet: 0.594 ± 0.023
0.798PheAsn: 0.798 ± 0.027
1.533PhePro: 1.533 ± 0.034
0.856PheGln: 0.856 ± 0.03
2.249PheArg: 2.249 ± 0.045
1.985PheSer: 1.985 ± 0.038
2.004PheThr: 2.004 ± 0.043
2.811PheVal: 2.811 ± 0.05
0.434PheTrp: 0.434 ± 0.02
0.764PheTyr: 0.764 ± 0.026
0.0PheXaa: 0.0 ± 0.0
Gly
11.539GlyAla: 11.539 ± 0.121
0.801GlyCys: 0.801 ± 0.029
4.696GlyAsp: 4.696 ± 0.061
4.601GlyGlu: 4.601 ± 0.058
3.758GlyPhe: 3.758 ± 0.063
8.085GlyGly: 8.085 ± 0.141
1.984GlyHis: 1.984 ± 0.043
4.442GlyIle: 4.442 ± 0.059
2.428GlyLys: 2.428 ± 0.046
10.994GlyLeu: 10.994 ± 0.119
1.886GlyMet: 1.886 ± 0.033
1.876GlyAsn: 1.876 ± 0.049
4.662GlyPro: 4.662 ± 0.059
2.749GlyGln: 2.749 ± 0.051
7.952GlyArg: 7.952 ± 0.081
4.516GlySer: 4.516 ± 0.073
5.663GlyThr: 5.663 ± 0.098
6.228GlyVal: 6.228 ± 0.086
1.301GlyTrp: 1.301 ± 0.037
2.2GlyTyr: 2.2 ± 0.057
0.0GlyXaa: 0.0 ± 0.0
His
2.767HisAla: 2.767 ± 0.052
0.187HisCys: 0.187 ± 0.012
1.261HisAsp: 1.261 ± 0.034
0.919HisGlu: 0.919 ± 0.026
0.702HisPhe: 0.702 ± 0.025
2.064HisGly: 2.064 ± 0.045
0.568HisHis: 0.568 ± 0.025
0.694HisIle: 0.694 ± 0.025
0.358HisLys: 0.358 ± 0.018
2.181HisLeu: 2.181 ± 0.049
0.357HisMet: 0.357 ± 0.013
0.345HisAsn: 0.345 ± 0.015
1.435HisPro: 1.435 ± 0.033
0.471HisGln: 0.471 ± 0.02
1.614HisArg: 1.614 ± 0.034
0.682HisSer: 0.682 ± 0.025
0.796HisThr: 0.796 ± 0.028
1.491HisVal: 1.491 ± 0.038
0.298HisTrp: 0.298 ± 0.017
0.487HisTyr: 0.487 ± 0.02
0.0HisXaa: 0.0 ± 0.0
Ile
6.79IleAla: 6.79 ± 0.078
0.384IleCys: 0.384 ± 0.016
3.099IleAsp: 3.099 ± 0.052
3.02IleGlu: 3.02 ± 0.057
1.173IlePhe: 1.173 ± 0.034
4.568IleGly: 4.568 ± 0.072
0.783IleHis: 0.783 ± 0.026
1.385IleIle: 1.385 ± 0.033
1.024IleLys: 1.024 ± 0.028
4.471IleLeu: 4.471 ± 0.07
0.633IleMet: 0.633 ± 0.023
0.957IleAsn: 0.957 ± 0.028
2.187IlePro: 2.187 ± 0.045
1.057IleGln: 1.057 ± 0.029
3.18IleArg: 3.18 ± 0.042
1.82IleSer: 1.82 ± 0.04
2.345IleThr: 2.345 ± 0.045
3.983IleVal: 3.983 ± 0.06
0.424IleTrp: 0.424 ± 0.019
0.764IleTyr: 0.764 ± 0.028
0.0IleXaa: 0.0 ± 0.0
Lys
3.894LysAla: 3.894 ± 0.072
0.123LysCys: 0.123 ± 0.009
1.398LysAsp: 1.398 ± 0.042
1.056LysGlu: 1.056 ± 0.032
0.634LysPhe: 0.634 ± 0.019
2.323LysGly: 2.323 ± 0.05
0.404LysHis: 0.404 ± 0.018
1.23LysIle: 1.23 ± 0.033
0.87LysLys: 0.87 ± 0.033
2.411LysLeu: 2.411 ± 0.049
0.498LysMet: 0.498 ± 0.022
0.589LysAsn: 0.589 ± 0.024
1.911LysPro: 1.911 ± 0.049
0.655LysGln: 0.655 ± 0.023
1.979LysArg: 1.979 ± 0.043
1.319LysSer: 1.319 ± 0.034
1.516LysThr: 1.516 ± 0.037
1.979LysVal: 1.979 ± 0.052
0.226LysTrp: 0.226 ± 0.015
0.408LysTyr: 0.408 ± 0.019
0.0LysXaa: 0.0 ± 0.0
Leu
16.554LeuAla: 16.554 ± 0.164
0.917LeuCys: 0.917 ± 0.028
6.782LeuAsp: 6.782 ± 0.089
4.534LeuGlu: 4.534 ± 0.073
3.388LeuPhe: 3.388 ± 0.056
9.404LeuGly: 9.404 ± 0.087
1.958LeuHis: 1.958 ± 0.044
4.268LeuIle: 4.268 ± 0.068
2.971LeuLys: 2.971 ± 0.055
9.1LeuLeu: 9.1 ± 0.123
2.039LeuMet: 2.039 ± 0.047
2.2LeuAsn: 2.2 ± 0.044
5.939LeuPro: 5.939 ± 0.075
2.346LeuGln: 2.346 ± 0.048
7.705LeuArg: 7.705 ± 0.105
5.902LeuSer: 5.902 ± 0.073
6.143LeuThr: 6.143 ± 0.073
8.7LeuVal: 8.7 ± 0.102
1.065LeuTrp: 1.065 ± 0.031
2.017LeuTyr: 2.017 ± 0.039
0.0LeuXaa: 0.0 ± 0.0
Met
2.754MetAla: 2.754 ± 0.047
0.112MetCys: 0.112 ± 0.01
0.881MetAsp: 0.881 ± 0.029
0.92MetGlu: 0.92 ± 0.031
0.477MetPhe: 0.477 ± 0.021
1.582MetGly: 1.582 ± 0.039
0.346MetHis: 0.346 ± 0.016
1.024MetIle: 1.024 ± 0.032
0.661MetLys: 0.661 ± 0.026
1.989MetLeu: 1.989 ± 0.04
0.517MetMet: 0.517 ± 0.024
0.596MetAsn: 0.596 ± 0.021
1.494MetPro: 1.494 ± 0.032
0.62MetGln: 0.62 ± 0.024
1.817MetArg: 1.817 ± 0.042
1.313MetSer: 1.313 ± 0.034
1.587MetThr: 1.587 ± 0.036
1.377MetVal: 1.377 ± 0.032
0.143MetTrp: 0.143 ± 0.01
0.192MetTyr: 0.192 ± 0.013
0.0MetXaa: 0.0 ± 0.0
Asn
2.943AsnAla: 2.943 ± 0.05
0.172AsnCys: 0.172 ± 0.012
1.128AsnAsp: 1.128 ± 0.031
0.875AsnGlu: 0.875 ± 0.031
0.691AsnPhe: 0.691 ± 0.026
2.061AsnGly: 2.061 ± 0.047
0.413AsnHis: 0.413 ± 0.02
0.92AsnIle: 0.92 ± 0.028
0.457AsnLys: 0.457 ± 0.02
2.232AsnLeu: 2.232 ± 0.045
0.398AsnMet: 0.398 ± 0.016
0.501AsnAsn: 0.501 ± 0.025
1.674AsnPro: 1.674 ± 0.036
0.557AsnGln: 0.557 ± 0.022
1.665AsnArg: 1.665 ± 0.038
0.817AsnSer: 0.817 ± 0.027
1.131AsnThr: 1.131 ± 0.037
1.621AsnVal: 1.621 ± 0.044
0.269AsnTrp: 0.269 ± 0.017
0.474AsnTyr: 0.474 ± 0.022
0.0AsnXaa: 0.0 ± 0.0
Pro
8.206ProAla: 8.206 ± 0.106
0.346ProCys: 0.346 ± 0.017
4.287ProAsp: 4.287 ± 0.07
4.005ProGlu: 4.005 ± 0.071
2.042ProPhe: 2.042 ± 0.041
5.618ProGly: 5.618 ± 0.071
1.153ProHis: 1.153 ± 0.033
2.192ProIle: 2.192 ± 0.041
1.541ProLys: 1.541 ± 0.04
5.138ProLeu: 5.138 ± 0.066
1.248ProMet: 1.248 ± 0.035
1.239ProAsn: 1.239 ± 0.036
3.261ProPro: 3.261 ± 0.073
1.45ProGln: 1.45 ± 0.035
3.728ProArg: 3.728 ± 0.057
2.863ProSer: 2.863 ± 0.052
2.81ProThr: 2.81 ± 0.044
4.956ProVal: 4.956 ± 0.07
0.754ProTrp: 0.754 ± 0.024
1.119ProTyr: 1.119 ± 0.033
0.0ProXaa: 0.0 ± 0.0
Gln
4.32GlnAla: 4.32 ± 0.063
0.15GlnCys: 0.15 ± 0.012
1.388GlnAsp: 1.388 ± 0.033
1.099GlnGlu: 1.099 ± 0.034
0.825GlnPhe: 0.825 ± 0.027
2.453GlnGly: 2.453 ± 0.042
0.503GlnHis: 0.503 ± 0.021
1.442GlnIle: 1.442 ± 0.038
0.729GlnLys: 0.729 ± 0.024
2.027GlnLeu: 2.027 ± 0.046
0.544GlnMet: 0.544 ± 0.021
0.67GlnAsn: 0.67 ± 0.021
1.522GlnPro: 1.522 ± 0.036
0.787GlnGln: 0.787 ± 0.028
2.111GlnArg: 2.111 ± 0.043
1.389GlnSer: 1.389 ± 0.036
1.635GlnThr: 1.635 ± 0.033
2.045GlnVal: 2.045 ± 0.045
0.272GlnTrp: 0.272 ± 0.014
0.451GlnTyr: 0.451 ± 0.02
0.0GlnXaa: 0.0 ± 0.0
Arg
10.548ArgAla: 10.548 ± 0.118
0.49ArgCys: 0.49 ± 0.023
4.797ArgAsp: 4.797 ± 0.068
4.164ArgGlu: 4.164 ± 0.07
3.169ArgPhe: 3.169 ± 0.052
5.737ArgGly: 5.737 ± 0.076
1.86ArgHis: 1.86 ± 0.041
4.209ArgIle: 4.209 ± 0.059
1.773ArgLys: 1.773 ± 0.038
9.161ArgLeu: 9.161 ± 0.118
1.835ArgMet: 1.835 ± 0.035
1.62ArgAsn: 1.62 ± 0.038
4.66ArgPro: 4.66 ± 0.067
2.187ArgGln: 2.187 ± 0.047
7.004ArgArg: 7.004 ± 0.099
3.739ArgSer: 3.739 ± 0.065
4.472ArgThr: 4.472 ± 0.069
5.759ArgVal: 5.759 ± 0.075
0.968ArgTrp: 0.968 ± 0.032
1.681ArgTyr: 1.681 ± 0.037
0.0ArgXaa: 0.0 ± 0.0
Ser
6.015SerAla: 6.015 ± 0.077
0.336SerCys: 0.336 ± 0.016
2.703SerAsp: 2.703 ± 0.045
2.457SerGlu: 2.457 ± 0.046
1.76SerPhe: 1.76 ± 0.038
5.322SerGly: 5.322 ± 0.078
0.903SerHis: 0.903 ± 0.024
2.053SerIle: 2.053 ± 0.039
1.146SerLys: 1.146 ± 0.032
5.279SerLeu: 5.279 ± 0.07
0.958SerMet: 0.958 ± 0.031
1.05SerAsn: 1.05 ± 0.032
2.816SerPro: 2.816 ± 0.044
1.309SerGln: 1.309 ± 0.031
3.359SerArg: 3.359 ± 0.056
2.188SerSer: 2.188 ± 0.048
2.401SerThr: 2.401 ± 0.051
3.75SerVal: 3.75 ± 0.053
0.552SerTrp: 0.552 ± 0.021
1.046SerTyr: 1.046 ± 0.03
0.0SerXaa: 0.0 ± 0.0
Thr
7.159ThrAla: 7.159 ± 0.084
0.415ThrCys: 0.415 ± 0.021
3.01ThrAsp: 3.01 ± 0.052
2.624ThrGlu: 2.624 ± 0.05
1.939ThrPhe: 1.939 ± 0.042
6.008ThrGly: 6.008 ± 0.092
1.032ThrHis: 1.032 ± 0.033
2.603ThrIle: 2.603 ± 0.06
1.347ThrLys: 1.347 ± 0.036
6.842ThrLeu: 6.842 ± 0.076
0.951ThrMet: 0.951 ± 0.027
1.186ThrAsn: 1.186 ± 0.041
3.624ThrPro: 3.624 ± 0.056
1.508ThrGln: 1.508 ± 0.032
4.023ThrArg: 4.023 ± 0.06
2.388ThrSer: 2.388 ± 0.048
3.016ThrThr: 3.016 ± 0.065
5.068ThrVal: 5.068 ± 0.092
0.696ThrTrp: 0.696 ± 0.023
1.273ThrTyr: 1.273 ± 0.035
0.0ThrXaa: 0.0 ± 0.0
Val
11.35ValAla: 11.35 ± 0.114
0.614ValCys: 0.614 ± 0.023
4.161ValAsp: 4.161 ± 0.068
4.557ValGlu: 4.557 ± 0.057
2.633ValPhe: 2.633 ± 0.053
6.064ValGly: 6.064 ± 0.078
1.414ValHis: 1.414 ± 0.041
3.443ValIle: 3.443 ± 0.05
1.863ValLys: 1.863 ± 0.046
7.811ValLeu: 7.811 ± 0.096
1.625ValMet: 1.625 ± 0.037
1.719ValAsn: 1.719 ± 0.04
4.56ValPro: 4.56 ± 0.062
1.994ValGln: 1.994 ± 0.045
5.794ValArg: 5.794 ± 0.085
4.142ValSer: 4.142 ± 0.064
5.074ValThr: 5.074 ± 0.083
6.613ValVal: 6.613 ± 0.084
0.839ValTrp: 0.839 ± 0.029
1.345ValTyr: 1.345 ± 0.035
0.0ValXaa: 0.0 ± 0.0
Trp
1.247TrpAla: 1.247 ± 0.035
0.143TrpCys: 0.143 ± 0.009
0.59TrpAsp: 0.59 ± 0.023
0.454TrpGlu: 0.454 ± 0.02
0.466TrpPhe: 0.466 ± 0.021
0.841TrpGly: 0.841 ± 0.029
0.294TrpHis: 0.294 ± 0.014
0.584TrpIle: 0.584 ± 0.023
0.307TrpLys: 0.307 ± 0.017
1.472TrpLeu: 1.472 ± 0.04
0.298TrpMet: 0.298 ± 0.014
0.354TrpAsn: 0.354 ± 0.02
0.646TrpPro: 0.646 ± 0.023
0.408TrpGln: 0.408 ± 0.02
1.176TrpArg: 1.176 ± 0.03
0.762TrpSer: 0.762 ± 0.024
0.809TrpThr: 0.809 ± 0.027
0.744TrpVal: 0.744 ± 0.025
0.201TrpTrp: 0.201 ± 0.012
0.243TrpTyr: 0.243 ± 0.014
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.808TyrAla: 2.808 ± 0.045
0.189TyrCys: 0.189 ± 0.012
1.309TyrAsp: 1.309 ± 0.029
1.059TyrGlu: 1.059 ± 0.034
0.731TyrPhe: 0.731 ± 0.028
2.212TyrGly: 2.212 ± 0.054
0.389TyrHis: 0.389 ± 0.019
0.609TyrIle: 0.609 ± 0.023
0.407TyrLys: 0.407 ± 0.019
2.046TyrLeu: 2.046 ± 0.044
0.297TyrMet: 0.297 ± 0.015
0.459TyrAsn: 0.459 ± 0.019
1.0TyrPro: 1.0 ± 0.03
0.491TyrGln: 0.491 ± 0.02
1.821TyrArg: 1.821 ± 0.039
0.809TyrSer: 0.809 ± 0.027
1.017TyrThr: 1.017 ± 0.029
1.527TyrVal: 1.527 ± 0.04
0.287TyrTrp: 0.287 ± 0.015
0.442TyrTyr: 0.442 ± 0.02
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4109 proteins (1270044 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski