Amino acid dipepetide frequency for Streptococcus sp. DD10

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.308AlaAla: 5.308 ± 0.149
0.489AlaCys: 0.489 ± 0.027
4.104AlaAsp: 4.104 ± 0.094
5.061AlaGlu: 5.061 ± 0.11
3.182AlaPhe: 3.182 ± 0.091
5.417AlaGly: 5.417 ± 0.128
1.308AlaHis: 1.308 ± 0.045
5.578AlaIle: 5.578 ± 0.111
4.96AlaLys: 4.96 ± 0.106
7.156AlaLeu: 7.156 ± 0.119
1.801AlaMet: 1.801 ± 0.054
3.104AlaAsn: 3.104 ± 0.094
2.128AlaPro: 2.128 ± 0.072
3.185AlaGln: 3.185 ± 0.085
2.997AlaArg: 2.997 ± 0.071
4.695AlaSer: 4.695 ± 0.092
4.258AlaThr: 4.258 ± 0.101
5.299AlaVal: 5.299 ± 0.109
0.62AlaTrp: 0.62 ± 0.038
2.658AlaTyr: 2.658 ± 0.076
0.0AlaXaa: 0.0 ± 0.0
Cys
0.279CysAla: 0.279 ± 0.023
0.048CysCys: 0.048 ± 0.011
0.286CysAsp: 0.286 ± 0.028
0.258CysGlu: 0.258 ± 0.023
0.266CysPhe: 0.266 ± 0.023
0.485CysGly: 0.485 ± 0.035
0.156CysHis: 0.156 ± 0.019
0.307CysIle: 0.307 ± 0.025
0.22CysLys: 0.22 ± 0.021
0.609CysLeu: 0.609 ± 0.036
0.142CysMet: 0.142 ± 0.014
0.204CysAsn: 0.204 ± 0.02
0.24CysPro: 0.24 ± 0.025
0.316CysGln: 0.316 ± 0.026
0.211CysArg: 0.211 ± 0.018
0.357CysSer: 0.357 ± 0.03
0.233CysThr: 0.233 ± 0.023
0.314CysVal: 0.314 ± 0.026
0.034CysTrp: 0.034 ± 0.009
0.219CysTyr: 0.219 ± 0.019
0.0CysXaa: 0.0 ± 0.0
Asp
3.633AspAla: 3.633 ± 0.088
0.33AspCys: 0.33 ± 0.025
2.603AspAsp: 2.603 ± 0.079
4.145AspGlu: 4.145 ± 0.103
3.439AspPhe: 3.439 ± 0.082
3.846AspGly: 3.846 ± 0.103
0.936AspHis: 0.936 ± 0.044
4.267AspIle: 4.267 ± 0.083
3.949AspLys: 3.949 ± 0.082
5.731AspLeu: 5.731 ± 0.112
1.409AspMet: 1.409 ± 0.05
2.384AspAsn: 2.384 ± 0.082
1.51AspPro: 1.51 ± 0.052
2.02AspGln: 2.02 ± 0.064
2.036AspArg: 2.036 ± 0.058
3.041AspSer: 3.041 ± 0.081
2.855AspThr: 2.855 ± 0.064
3.756AspVal: 3.756 ± 0.089
0.698AspTrp: 0.698 ± 0.036
2.786AspTyr: 2.786 ± 0.074
0.0AspXaa: 0.0 ± 0.0
Glu
5.443GluAla: 5.443 ± 0.109
0.259GluCys: 0.259 ± 0.019
3.768GluAsp: 3.768 ± 0.091
6.433GluGlu: 6.433 ± 0.123
2.867GluPhe: 2.867 ± 0.075
3.875GluGly: 3.875 ± 0.089
1.274GluHis: 1.274 ± 0.053
5.676GluIle: 5.676 ± 0.109
6.301GluLys: 6.301 ± 0.108
7.534GluLeu: 7.534 ± 0.138
1.99GluMet: 1.99 ± 0.057
4.289GluAsn: 4.289 ± 0.088
1.569GluPro: 1.569 ± 0.048
3.066GluGln: 3.066 ± 0.07
3.557GluArg: 3.557 ± 0.089
3.637GluSer: 3.637 ± 0.114
4.177GluThr: 4.177 ± 0.099
5.063GluVal: 5.063 ± 0.124
0.618GluTrp: 0.618 ± 0.033
2.061GluTyr: 2.061 ± 0.064
0.0GluXaa: 0.0 ± 0.0
Phe
3.272PheAla: 3.272 ± 0.077
0.27PheCys: 0.27 ± 0.022
2.995PheAsp: 2.995 ± 0.078
3.352PheGlu: 3.352 ± 0.083
2.123PhePhe: 2.123 ± 0.074
3.157PheGly: 3.157 ± 0.083
0.886PheHis: 0.886 ± 0.037
3.144PheIle: 3.144 ± 0.077
2.448PheLys: 2.448 ± 0.061
4.834PheLeu: 4.834 ± 0.113
1.107PheMet: 1.107 ± 0.045
1.977PheAsn: 1.977 ± 0.062
1.595PhePro: 1.595 ± 0.057
1.618PheGln: 1.618 ± 0.056
1.668PheArg: 1.668 ± 0.053
3.498PheSer: 3.498 ± 0.079
2.569PheThr: 2.569 ± 0.072
3.125PheVal: 3.125 ± 0.078
0.46PheTrp: 0.46 ± 0.03
1.924PheTyr: 1.924 ± 0.069
0.0PheXaa: 0.0 ± 0.0
Gly
4.601GlyAla: 4.601 ± 0.116
0.391GlyCys: 0.391 ± 0.032
3.326GlyAsp: 3.326 ± 0.081
3.955GlyGlu: 3.955 ± 0.098
3.324GlyPhe: 3.324 ± 0.081
4.322GlyGly: 4.322 ± 0.122
1.258GlyHis: 1.258 ± 0.049
5.09GlyIle: 5.09 ± 0.117
4.58GlyLys: 4.58 ± 0.096
6.705GlyLeu: 6.705 ± 0.125
1.769GlyMet: 1.769 ± 0.058
2.876GlyAsn: 2.876 ± 0.068
1.343GlyPro: 1.343 ± 0.053
2.951GlyGln: 2.951 ± 0.084
2.887GlyArg: 2.887 ± 0.07
3.667GlySer: 3.667 ± 0.082
3.796GlyThr: 3.796 ± 0.081
4.951GlyVal: 4.951 ± 0.101
0.65GlyTrp: 0.65 ± 0.035
2.659GlyTyr: 2.659 ± 0.071
0.0GlyXaa: 0.0 ± 0.0
His
1.208HisAla: 1.208 ± 0.047
0.155HisCys: 0.155 ± 0.019
0.945HisAsp: 0.945 ± 0.04
1.149HisGlu: 1.149 ± 0.051
1.164HisPhe: 1.164 ± 0.052
1.231HisGly: 1.231 ± 0.049
0.618HisHis: 0.618 ± 0.028
1.308HisIle: 1.308 ± 0.048
1.002HisLys: 1.002 ± 0.04
2.118HisLeu: 2.118 ± 0.065
0.37HisMet: 0.37 ± 0.023
0.727HisAsn: 0.727 ± 0.034
0.929HisPro: 0.929 ± 0.046
1.013HisGln: 1.013 ± 0.042
0.947HisArg: 0.947 ± 0.039
1.109HisSer: 1.109 ± 0.041
0.956HisThr: 0.956 ± 0.052
1.194HisVal: 1.194 ± 0.046
0.156HisTrp: 0.156 ± 0.017
0.981HisTyr: 0.981 ± 0.04
0.0HisXaa: 0.0 ± 0.0
Ile
5.836IleAla: 5.836 ± 0.112
0.48IleCys: 0.48 ± 0.031
4.186IleAsp: 4.186 ± 0.103
5.163IleGlu: 5.163 ± 0.111
3.367IlePhe: 3.367 ± 0.099
4.731IleGly: 4.731 ± 0.105
1.373IleHis: 1.373 ± 0.051
4.752IleIle: 4.752 ± 0.1
4.335IleLys: 4.335 ± 0.097
7.563IleLeu: 7.563 ± 0.154
1.411IleMet: 1.411 ± 0.056
3.057IleAsn: 3.057 ± 0.085
2.727IlePro: 2.727 ± 0.068
2.91IleGln: 2.91 ± 0.079
3.102IleArg: 3.102 ± 0.071
4.944IleSer: 4.944 ± 0.087
3.983IleThr: 3.983 ± 0.084
4.878IleVal: 4.878 ± 0.089
0.609IleTrp: 0.609 ± 0.031
2.58IleTyr: 2.58 ± 0.076
0.0IleXaa: 0.0 ± 0.0
Lys
4.576LysAla: 4.576 ± 0.106
0.174LysCys: 0.174 ± 0.02
3.804LysAsp: 3.804 ± 0.103
6.243LysGlu: 6.243 ± 0.108
1.97LysPhe: 1.97 ± 0.07
3.901LysGly: 3.901 ± 0.093
1.137LysHis: 1.137 ± 0.044
4.77LysIle: 4.77 ± 0.096
5.415LysLys: 5.415 ± 0.109
5.902LysLeu: 5.902 ± 0.108
1.954LysMet: 1.954 ± 0.067
3.626LysAsn: 3.626 ± 0.077
1.979LysPro: 1.979 ± 0.069
2.755LysGln: 2.755 ± 0.078
3.095LysArg: 3.095 ± 0.083
3.674LysSer: 3.674 ± 0.092
3.864LysThr: 3.864 ± 0.094
4.502LysVal: 4.502 ± 0.109
0.558LysTrp: 0.558 ± 0.03
2.173LysTyr: 2.173 ± 0.074
0.0LysXaa: 0.0 ± 0.0
Leu
8.698LeuAla: 8.698 ± 0.138
0.489LeuCys: 0.489 ± 0.031
5.816LeuAsp: 5.816 ± 0.106
7.534LeuGlu: 7.534 ± 0.15
4.551LeuPhe: 4.551 ± 0.13
6.523LeuGly: 6.523 ± 0.125
1.778LeuHis: 1.778 ± 0.055
6.568LeuIle: 6.568 ± 0.132
6.133LeuLys: 6.133 ± 0.11
10.297LeuLeu: 10.297 ± 0.236
2.313LeuMet: 2.313 ± 0.068
4.241LeuAsn: 4.241 ± 0.085
4.017LeuPro: 4.017 ± 0.091
3.644LeuGln: 3.644 ± 0.093
3.796LeuArg: 3.796 ± 0.096
7.46LeuSer: 7.46 ± 0.156
6.276LeuThr: 6.276 ± 0.096
7.088LeuVal: 7.088 ± 0.101
0.679LeuTrp: 0.679 ± 0.036
3.352LeuTyr: 3.352 ± 0.077
0.0LeuXaa: 0.0 ± 0.0
Met
1.785MetAla: 1.785 ± 0.057
0.103MetCys: 0.103 ± 0.013
1.4MetAsp: 1.4 ± 0.05
1.626MetGlu: 1.626 ± 0.054
0.81MetPhe: 0.81 ± 0.042
1.624MetGly: 1.624 ± 0.061
0.33MetHis: 0.33 ± 0.025
1.736MetIle: 1.736 ± 0.061
2.043MetLys: 2.043 ± 0.056
2.182MetLeu: 2.182 ± 0.067
0.629MetMet: 0.629 ± 0.037
1.252MetAsn: 1.252 ± 0.04
0.767MetPro: 0.767 ± 0.038
0.862MetGln: 0.862 ± 0.036
1.032MetArg: 1.032 ± 0.041
1.565MetSer: 1.565 ± 0.053
1.844MetThr: 1.844 ± 0.05
1.642MetVal: 1.642 ± 0.055
0.179MetTrp: 0.179 ± 0.017
0.567MetTyr: 0.567 ± 0.029
0.0MetXaa: 0.0 ± 0.0
Asn
2.954AsnAla: 2.954 ± 0.083
0.243AsnCys: 0.243 ± 0.02
2.226AsnAsp: 2.226 ± 0.061
2.699AsnGlu: 2.699 ± 0.073
2.187AsnPhe: 2.187 ± 0.07
3.464AsnGly: 3.464 ± 0.098
1.1AsnHis: 1.1 ± 0.042
3.23AsnIle: 3.23 ± 0.073
2.674AsnLys: 2.674 ± 0.066
4.839AsnLeu: 4.839 ± 0.102
1.132AsnMet: 1.132 ± 0.042
1.92AsnAsn: 1.92 ± 0.061
2.315AsnPro: 2.315 ± 0.066
2.482AsnGln: 2.482 ± 0.072
2.395AsnArg: 2.395 ± 0.061
2.583AsnSer: 2.583 ± 0.074
2.254AsnThr: 2.254 ± 0.062
2.709AsnVal: 2.709 ± 0.072
0.517AsnTrp: 0.517 ± 0.026
1.96AsnTyr: 1.96 ± 0.065
0.0AsnXaa: 0.0 ± 0.0
Pro
2.414ProAla: 2.414 ± 0.081
0.144ProCys: 0.144 ± 0.017
1.945ProAsp: 1.945 ± 0.059
2.858ProGlu: 2.858 ± 0.087
1.65ProPhe: 1.65 ± 0.06
1.672ProGly: 1.672 ± 0.059
0.748ProHis: 0.748 ± 0.04
2.434ProIle: 2.434 ± 0.062
1.924ProLys: 1.924 ± 0.065
2.848ProLeu: 2.848 ± 0.073
0.675ProMet: 0.675 ± 0.036
1.627ProAsn: 1.627 ± 0.053
0.56ProPro: 0.56 ± 0.033
1.345ProGln: 1.345 ± 0.052
1.151ProArg: 1.151 ± 0.042
2.135ProSer: 2.135 ± 0.064
2.079ProThr: 2.079 ± 0.066
2.542ProVal: 2.542 ± 0.07
0.281ProTrp: 0.281 ± 0.022
1.371ProTyr: 1.371 ± 0.05
0.0ProXaa: 0.0 ± 0.0
Gln
3.621GlnAla: 3.621 ± 0.08
0.124GlnCys: 0.124 ± 0.015
2.169GlnAsp: 2.169 ± 0.064
3.711GlnGlu: 3.711 ± 0.102
1.8GlnPhe: 1.8 ± 0.06
2.336GlnGly: 2.336 ± 0.077
0.819GlnHis: 0.819 ± 0.04
2.905GlnIle: 2.905 ± 0.071
2.821GlnLys: 2.821 ± 0.081
4.305GlnLeu: 4.305 ± 0.094
0.936GlnMet: 0.936 ± 0.042
1.899GlnAsn: 1.899 ± 0.068
1.174GlnPro: 1.174 ± 0.053
1.668GlnGln: 1.668 ± 0.06
1.498GlnArg: 1.498 ± 0.054
2.386GlnSer: 2.386 ± 0.077
2.505GlnThr: 2.505 ± 0.07
3.386GlnVal: 3.386 ± 0.08
0.311GlnTrp: 0.311 ± 0.027
1.387GlnTyr: 1.387 ± 0.05
0.0GlnXaa: 0.0 ± 0.0
Arg
2.72ArgAla: 2.72 ± 0.071
0.219ArgCys: 0.219 ± 0.024
2.37ArgAsp: 2.37 ± 0.065
3.413ArgGlu: 3.413 ± 0.086
2.112ArgPhe: 2.112 ± 0.067
2.448ArgGly: 2.448 ± 0.065
0.844ArgHis: 0.844 ± 0.04
3.057ArgIle: 3.057 ± 0.072
3.112ArgLys: 3.112 ± 0.084
4.228ArgLeu: 4.228 ± 0.097
1.228ArgMet: 1.228 ± 0.047
2.089ArgAsn: 2.089 ± 0.061
1.325ArgPro: 1.325 ± 0.051
1.92ArgGln: 1.92 ± 0.067
2.114ArgArg: 2.114 ± 0.063
2.132ArgSer: 2.132 ± 0.062
2.045ArgThr: 2.045 ± 0.07
2.938ArgVal: 2.938 ± 0.083
0.302ArgTrp: 0.302 ± 0.025
1.805ArgTyr: 1.805 ± 0.061
0.0ArgXaa: 0.0 ± 0.0
Ser
3.717SerAla: 3.717 ± 0.092
0.304SerCys: 0.304 ± 0.024
3.347SerAsp: 3.347 ± 0.084
3.901SerGlu: 3.901 ± 0.113
3.157SerPhe: 3.157 ± 0.082
4.372SerGly: 4.372 ± 0.088
1.302SerHis: 1.302 ± 0.049
4.615SerIle: 4.615 ± 0.11
3.946SerLys: 3.946 ± 0.089
6.792SerLeu: 6.792 ± 0.138
1.455SerMet: 1.455 ± 0.047
2.8SerAsn: 2.8 ± 0.063
1.917SerPro: 1.917 ± 0.051
3.111SerGln: 3.111 ± 0.09
2.656SerArg: 2.656 ± 0.069
4.445SerSer: 4.445 ± 0.143
3.416SerThr: 3.416 ± 0.165
4.111SerVal: 4.111 ± 0.164
0.593SerTrp: 0.593 ± 0.036
2.635SerTyr: 2.635 ± 0.073
0.0SerXaa: 0.0 ± 0.0
Thr
4.384ThrAla: 4.384 ± 0.103
0.304ThrCys: 0.304 ± 0.024
3.306ThrAsp: 3.306 ± 0.082
3.693ThrGlu: 3.693 ± 0.094
2.532ThrPhe: 2.532 ± 0.068
4.265ThrGly: 4.265 ± 0.098
1.105ThrHis: 1.105 ± 0.045
4.656ThrIle: 4.656 ± 0.09
3.185ThrLys: 3.185 ± 0.08
5.546ThrLeu: 5.546 ± 0.103
1.089ThrMet: 1.089 ± 0.049
2.638ThrAsn: 2.638 ± 0.067
2.462ThrPro: 2.462 ± 0.081
1.936ThrGln: 1.936 ± 0.058
2.036ThrArg: 2.036 ± 0.062
3.985ThrSer: 3.985 ± 0.166
3.455ThrThr: 3.455 ± 0.095
4.338ThrVal: 4.338 ± 0.105
0.506ThrTrp: 0.506 ± 0.028
2.269ThrTyr: 2.269 ± 0.058
0.0ThrXaa: 0.0 ± 0.0
Val
5.799ValAla: 5.799 ± 0.131
0.394ValCys: 0.394 ± 0.027
4.056ValAsp: 4.056 ± 0.092
5.246ValGlu: 5.246 ± 0.108
3.086ValPhe: 3.086 ± 0.091
4.431ValGly: 4.431 ± 0.116
1.22ValHis: 1.22 ± 0.053
4.946ValIle: 4.946 ± 0.095
4.404ValLys: 4.404 ± 0.095
6.902ValLeu: 6.902 ± 0.117
1.563ValMet: 1.563 ± 0.057
2.933ValAsn: 2.933 ± 0.072
2.249ValPro: 2.249 ± 0.074
2.258ValGln: 2.258 ± 0.057
2.887ValArg: 2.887 ± 0.074
4.578ValSer: 4.578 ± 0.142
4.726ValThr: 4.726 ± 0.087
5.138ValVal: 5.138 ± 0.119
0.529ValTrp: 0.529 ± 0.032
2.347ValTyr: 2.347 ± 0.061
0.0ValXaa: 0.0 ± 0.0
Trp
0.526TrpAla: 0.526 ± 0.027
0.05TrpCys: 0.05 ± 0.009
0.448TrpAsp: 0.448 ± 0.031
0.657TrpGlu: 0.657 ± 0.036
0.453TrpPhe: 0.453 ± 0.033
0.615TrpGly: 0.615 ± 0.036
0.163TrpHis: 0.163 ± 0.017
0.595TrpIle: 0.595 ± 0.027
0.547TrpLys: 0.547 ± 0.033
0.949TrpLeu: 0.949 ± 0.043
0.238TrpMet: 0.238 ± 0.022
0.51TrpAsn: 0.51 ± 0.031
0.158TrpPro: 0.158 ± 0.019
0.442TrpGln: 0.442 ± 0.03
0.373TrpArg: 0.373 ± 0.025
0.51TrpSer: 0.51 ± 0.037
0.496TrpThr: 0.496 ± 0.03
0.501TrpVal: 0.501 ± 0.027
0.11TrpTrp: 0.11 ± 0.013
0.389TrpTyr: 0.389 ± 0.025
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.533TyrAla: 2.533 ± 0.072
0.217TyrCys: 0.217 ± 0.023
2.276TyrAsp: 2.276 ± 0.06
2.359TyrGlu: 2.359 ± 0.062
1.984TyrPhe: 1.984 ± 0.077
2.386TyrGly: 2.386 ± 0.069
0.92TyrHis: 0.92 ± 0.043
2.35TyrIle: 2.35 ± 0.073
2.073TyrLys: 2.073 ± 0.063
4.216TyrLeu: 4.216 ± 0.104
0.766TyrMet: 0.766 ± 0.036
1.729TyrAsn: 1.729 ± 0.067
1.439TyrPro: 1.439 ± 0.052
2.258TyrGln: 2.258 ± 0.072
1.984TyrArg: 1.984 ± 0.064
2.19TyrSer: 2.19 ± 0.074
1.906TyrThr: 1.906 ± 0.061
2.215TyrVal: 2.215 ± 0.069
0.318TyrTrp: 0.318 ± 0.026
1.691TyrTyr: 1.691 ± 0.063
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2052 proteins (562896 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski