Simian T-lymphotropic virus 1
Average proteome isoelectric point is 7.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q9WS53|Q9WS53_9STL1 Env polyprotein OS=Simian T-lymphotropic virus 1 OX=33747 PE=4 SV=1
MM1 pKa = 7.74 RR2 pKa = 11.84 KK3 pKa = 9.61 YY4 pKa = 10.63 SPFRR8 pKa = 11.84 NGYY11 pKa = 7.91 MEE13 pKa = 4.35 PTLGQHH19 pKa = 6.94 LPTLSFPDD27 pKa = 3.77 PGLRR31 pKa = 11.84 PQNLYY36 pKa = 8.25 TLWGDD41 pKa = 4.07 SVVCLYY47 pKa = 10.65 LYY49 pKa = 10.33 QLSPPITWPLPPHH62 pKa = 7.02 VIFCHH67 pKa = 6.21 PGQLGAFLTNVPYY80 pKa = 10.94 KK81 pKa = 10.66 RR82 pKa = 11.84 MEE84 pKa = 3.93 EE85 pKa = 3.84 LLYY88 pKa = 10.66 KK89 pKa = 10.55 ISLTTGALIILPEE102 pKa = 4.78 DD103 pKa = 3.97 CLPTTLFQPARR114 pKa = 11.84 APVTLTAWQNGLLPFHH130 pKa = 6.75 STLTTPGLIWTFTDD144 pKa = 3.88 GTPMVSGPCPKK155 pKa = 10.26 DD156 pKa = 3.49 GQPSLVLQSSSFIFHH171 pKa = 7.35 KK172 pKa = 10.59 FQTKK176 pKa = 9.83 AYY178 pKa = 8.81 HH179 pKa = 6.37 PSFLLSHH186 pKa = 6.6 GLIQYY191 pKa = 10.76 SSFHH195 pKa = 5.98 NLHH198 pKa = 6.68 LLFEE202 pKa = 5.28 EE203 pKa = 4.66 YY204 pKa = 10.86 TNIPVSLLFNEE215 pKa = 5.05 KK216 pKa = 9.61 EE217 pKa = 4.34 ANDD220 pKa = 4.02 TDD222 pKa = 4.35 HH223 pKa = 7.18 EE224 pKa = 4.3 PQISPGGLEE233 pKa = 4.2 PPAEE237 pKa = 3.88 KK238 pKa = 10.27 HH239 pKa = 5.35 FRR241 pKa = 11.84 EE242 pKa = 4.52 TEE244 pKa = 3.99 VV245 pKa = 3.0
Molecular weight: 27.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.504
IPC2_protein 5.626
IPC_protein 5.677
Toseland 6.173
ProMoST 6.059
Dawson 5.97
Bjellqvist 5.957
Wikipedia 5.995
Rodwell 5.957
Grimsley 6.351
Solomon 5.97
Lehninger 5.97
Nozaki 6.249
DTASelect 6.44
Thurlkill 6.465
EMBOSS 6.427
Sillero 6.351
Patrickios 2.918
IPC_peptide 5.982
IPC2_peptide 6.364
IPC2.peptide.svr19 6.263
Protein with the highest isoelectric point:
>tr|Q9WS54|Q9WS54_9STL1 Protease OS=Simian T-lymphotropic virus 1 OX=33747 PE=4 SV=1
MM1 pKa = 6.99 QLAHH5 pKa = 7.39 ILQPIRR11 pKa = 11.84 QAFSQCTILQYY22 pKa = 10.34 MDD24 pKa = 6.42 DD25 pKa = 4.46 ILLASPSHH33 pKa = 7.19 KK34 pKa = 10.39 DD35 pKa = 3.33 LQLLSEE41 pKa = 4.32 VTMASLISHH50 pKa = 7.03 GLPVSEE56 pKa = 5.77 NKK58 pKa = 8.15 TQQTPGTIKK67 pKa = 10.65 FLGQIISPNHH77 pKa = 4.96 LTYY80 pKa = 10.85 DD81 pKa = 3.91 AVPTVPIRR89 pKa = 11.84 SRR91 pKa = 11.84 WALPEE96 pKa = 3.93 LQALLGEE103 pKa = 4.47 IQWVSKK109 pKa = 8.24 GTPTLRR115 pKa = 11.84 QPLHH119 pKa = 6.34 SLYY122 pKa = 10.66 CALQRR127 pKa = 11.84 HH128 pKa = 5.66 TDD130 pKa = 3.46 PRR132 pKa = 11.84 DD133 pKa = 3.62 QICLNPSQVQSLVQLRR149 pKa = 11.84 QALSQNCRR157 pKa = 11.84 SRR159 pKa = 11.84 LVQTVPLLGLVMLTLTGTTTVVFQSKK185 pKa = 7.33 QQWPLVWLHH194 pKa = 6.78 APLPHH199 pKa = 6.99 TSQCPWGQLLASAVLLLDD217 pKa = 4.16 KK218 pKa = 10.45 YY219 pKa = 8.72 TLQSYY224 pKa = 10.23 GLLCQTIHH232 pKa = 6.81 HH233 pKa = 6.74 NISTQTFDD241 pKa = 4.64 QFIQTSDD248 pKa = 3.57 HH249 pKa = 6.49 PSVPILYY256 pKa = 9.26 HH257 pKa = 5.76 HH258 pKa = 6.23 SHH260 pKa = 6.6 RR261 pKa = 11.84 FKK263 pKa = 11.3 NLGAQTGEE271 pKa = 3.76 LWNTFLKK278 pKa = 9.77 TAAPLAPVKK287 pKa = 10.77 ALMPMFTLSPVIINAAPCLFSDD309 pKa = 4.86 GSTSRR314 pKa = 11.84 AAYY317 pKa = 9.42 ILWDD321 pKa = 3.53 KK322 pKa = 11.02 HH323 pKa = 6.54 ILSQKK328 pKa = 10.16 SFPLPPPHH336 pKa = 7.31 KK337 pKa = 9.2 SAQRR341 pKa = 11.84 AEE343 pKa = 4.27 LLGLLHH349 pKa = 6.71 GLSSAHH355 pKa = 6.23 SWRR358 pKa = 11.84 CLNIFLDD365 pKa = 4.03 SKK367 pKa = 11.18 YY368 pKa = 10.2 LYY370 pKa = 10.25 HH371 pKa = 6.84 YY372 pKa = 10.73 LRR374 pKa = 11.84 TLALGTFQGRR384 pKa = 11.84 SSQAPFQDD392 pKa = 3.7 PLPRR396 pKa = 11.84 LLSRR400 pKa = 11.84 KK401 pKa = 8.61 VVYY404 pKa = 9.83 LHH406 pKa = 6.55 HH407 pKa = 6.57 VRR409 pKa = 11.84 SHH411 pKa = 6.33 TNLPDD416 pKa = 5.24 PITRR420 pKa = 11.84 LNALTDD426 pKa = 3.78 ALLITPVLQLSPAEE440 pKa = 4.08 LHH442 pKa = 6.79 SLTHH446 pKa = 6.75 CGQAALTLQGATTTEE461 pKa = 3.61 ASNILRR467 pKa = 11.84 SCHH470 pKa = 5.84 ACRR473 pKa = 11.84 KK474 pKa = 9.68 NNPQHH479 pKa = 5.62 QMPRR483 pKa = 11.84 GHH485 pKa = 6.92 IRR487 pKa = 11.84 RR488 pKa = 11.84 GLLPNHH494 pKa = 6.78 IWQGDD499 pKa = 3.53 ITHH502 pKa = 7.09 FKK504 pKa = 10.36 YY505 pKa = 10.9 KK506 pKa = 8.54 NTLYY510 pKa = 10.61 RR511 pKa = 11.84 LHH513 pKa = 6.88 VWVDD517 pKa = 3.4 TFSGAISATQKK528 pKa = 10.74 KK529 pKa = 9.76 KK530 pKa = 8.78 EE531 pKa = 4.25 TSSEE535 pKa = 4.27 AISSLLQAIAYY546 pKa = 8.61 LGKK549 pKa = 9.73 PNYY552 pKa = 9.76 INTDD556 pKa = 3.0 NGPAYY561 pKa = 9.83 ISQDD565 pKa = 3.65 FLDD568 pKa = 4.16 MCTPLAIRR576 pKa = 11.84 HH577 pKa = 4.98 TTHH580 pKa = 5.93 VPYY583 pKa = 10.85 NPTSSGLVEE592 pKa = 4.4 RR593 pKa = 11.84 SNGILKK599 pKa = 8.91 TLLYY603 pKa = 10.45 KK604 pKa = 10.93 YY605 pKa = 9.32 FTDD608 pKa = 5.39 KK609 pKa = 10.95 PDD611 pKa = 3.67 LPMDD615 pKa = 4.1 NALSIALWTINHH627 pKa = 6.38 LNVLTHH633 pKa = 5.36 CHH635 pKa = 4.4 KK636 pKa = 9.75 TRR638 pKa = 11.84 WQLHH642 pKa = 5.27 HH643 pKa = 6.85 SPRR646 pKa = 11.84 LQPIPEE652 pKa = 4.12 TRR654 pKa = 11.84 SLSNKK659 pKa = 5.47 QTHH662 pKa = 5.75 WYY664 pKa = 8.91 YY665 pKa = 11.25 FKK667 pKa = 11.28 LPGLNSRR674 pKa = 11.84 QWKK677 pKa = 9.58 GPQEE681 pKa = 4.28 ALQEE685 pKa = 4.25 AAGPALIPVIASSAQWIPWRR705 pKa = 11.84 LLKK708 pKa = 10.48 RR709 pKa = 11.84 AACPRR714 pKa = 11.84 PVGAPPIPKK723 pKa = 10.07 KK724 pKa = 9.95 KK725 pKa = 7.01 TTNTMGKK732 pKa = 9.76 FLTTLILFFQFCPLILGDD750 pKa = 4.58 YY751 pKa = 10.57 SPSCCTLTIGVSSYY765 pKa = 10.86 HH766 pKa = 6.73 SKK768 pKa = 10.17 PCNPAQPVCSWTLDD782 pKa = 3.88 LLALSADD789 pKa = 3.67 QALQPPCPNLVSYY802 pKa = 10.82 SSYY805 pKa = 9.9 HH806 pKa = 5.03 ATYY809 pKa = 10.81 SLYY812 pKa = 10.83 LFPHH816 pKa = 7.5 WIKK819 pKa = 10.78 KK820 pKa = 8.39 PNRR823 pKa = 11.84 NGGGYY828 pKa = 10.33 YY829 pKa = 9.83 SASYY833 pKa = 10.97 SDD835 pKa = 4.19 PCSLKK840 pKa = 10.57 CPYY843 pKa = 10.0 LGCQSWTCPYY853 pKa = 9.29 TGAISSPYY861 pKa = 9.66 WKK863 pKa = 10.1 FQQDD867 pKa = 3.56 VNFTQEE873 pKa = 3.84 VSRR876 pKa = 11.84 LTINLHH882 pKa = 5.47 FSKK885 pKa = 10.77 CGFPFSLLIDD895 pKa = 3.93 APGYY899 pKa = 10.57 DD900 pKa = 4.9 PIWFLNTEE908 pKa = 4.47 PSQLPPTAPPLLPHH922 pKa = 6.84 SNLDD926 pKa = 3.99 HH927 pKa = 7.3 ILEE930 pKa = 4.41 PSIPWKK936 pKa = 10.74 SKK938 pKa = 10.61 LLTPVQLTLQSTNYY952 pKa = 8.58 TCIVCIDD959 pKa = 3.86 RR960 pKa = 11.84 ASLSTWHH967 pKa = 6.34 VLYY970 pKa = 10.3 FPNVSVPSSPSTPLLYY986 pKa = 10.08 PSLALPAPHH995 pKa = 6.68 LTLPFNWTHH1004 pKa = 6.49 CFNPQIQAIVSSPCHH1019 pKa = 6.19 NSLILPPFSLSPVPTLGSRR1038 pKa = 11.84 SRR1040 pKa = 11.84 RR1041 pKa = 11.84 AVPVAVWLVSALAMGAGMAGGITGSMSLASGRR1073 pKa = 11.84 SLLHH1077 pKa = 6.58 EE1078 pKa = 4.11 VDD1080 pKa = 4.21 KK1081 pKa = 11.3 DD1082 pKa = 3.49 ISQLTQAIVKK1092 pKa = 8.27 NHH1094 pKa = 6.09 KK1095 pKa = 9.92 NLLKK1099 pKa = 9.97 IAQYY1103 pKa = 10.57 AAQNRR1108 pKa = 11.84 RR1109 pKa = 11.84 GLDD1112 pKa = 3.36 LLFWEE1117 pKa = 5.19 QGGLCKK1123 pKa = 10.25 ALQEE1127 pKa = 4.27 QCCFLNITNSHH1138 pKa = 4.81 VSILQEE1144 pKa = 4.04 RR1145 pKa = 11.84 PPLEE1149 pKa = 4.01 NRR1151 pKa = 11.84 VLTGWGLNWDD1161 pKa = 4.35 LGLSQWARR1169 pKa = 11.84 EE1170 pKa = 3.91 ALQTGITLVALLPLVILAGPCILRR1194 pKa = 11.84 QLRR1197 pKa = 11.84 HH1198 pKa = 5.3 LPSRR1202 pKa = 11.84 VRR1204 pKa = 11.84 HH1205 pKa = 4.99 PHH1207 pKa = 5.44 YY1208 pKa = 11.08 SLINPEE1214 pKa = 4.01 SCLL1217 pKa = 3.69
Molecular weight: 135.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.633
IPC2_protein 8.492
IPC_protein 8.302
Toseland 8.448
ProMoST 8.858
Dawson 9.121
Bjellqvist 9.253
Wikipedia 9.194
Rodwell 9.194
Grimsley 8.741
Solomon 9.151
Lehninger 9.121
Nozaki 9.414
DTASelect 9.048
Thurlkill 9.121
EMBOSS 9.268
Sillero 9.37
Patrickios 4.012
IPC_peptide 9.136
IPC2_peptide 8.361
IPC2.peptide.svr19 8.004
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
2056
166
1217
514.0
57.24
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.518 ± 0.966
2.529 ± 0.238
3.356 ± 0.557
2.918 ± 0.818
3.064 ± 0.614
5.01 ± 0.51
4.037 ± 0.619
5.01 ± 0.391
3.842 ± 0.329
13.765 ± 1.03
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.216 ± 0.13
3.696 ± 0.278
10.554 ± 1.727
6.469 ± 0.603
4.086 ± 0.381
8.123 ± 0.9
6.663 ± 1.109
4.037 ± 0.363
2.14 ± 0.212
2.967 ± 0.45
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here