Amino acid dipepetide frequency for Pseudomonas phage phi12

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.675AlaAla: 12.675 ± 3.122
0.528AlaCys: 0.528 ± 0.368
3.697AlaAsp: 3.697 ± 0.846
5.809AlaGlu: 5.809 ± 0.97
3.697AlaPhe: 3.697 ± 1.014
8.186AlaGly: 8.186 ± 1.984
0.792AlaHis: 0.792 ± 0.301
8.186AlaIle: 8.186 ± 1.223
4.753AlaLys: 4.753 ± 0.906
6.866AlaLeu: 6.866 ± 0.9
3.961AlaMet: 3.961 ± 0.9
2.905AlaAsn: 2.905 ± 0.799
3.697AlaPro: 3.697 ± 0.756
5.809AlaGln: 5.809 ± 1.872
4.225AlaArg: 4.225 ± 0.808
7.658AlaSer: 7.658 ± 1.932
3.433AlaThr: 3.433 ± 1.084
6.602AlaVal: 6.602 ± 1.555
2.641AlaTrp: 2.641 ± 2.466
4.225AlaTyr: 4.225 ± 0.903
0.0AlaXaa: 0.0 ± 0.0
Cys
0.0CysAla: 0.0 ± 0.0
0.0CysCys: 0.0 ± 0.0
0.0CysAsp: 0.0 ± 0.0
0.264CysGlu: 0.264 ± 0.198
0.0CysPhe: 0.0 ± 0.0
0.792CysGly: 0.792 ± 0.505
0.0CysHis: 0.0 ± 0.0
0.264CysIle: 0.264 ± 0.233
0.0CysLys: 0.0 ± 0.0
0.528CysLeu: 0.528 ± 0.416
0.792CysMet: 0.792 ± 0.46
0.528CysAsn: 0.528 ± 0.402
0.528CysPro: 0.528 ± 0.417
0.0CysGln: 0.0 ± 0.0
0.0CysArg: 0.0 ± 0.0
0.792CysSer: 0.792 ± 0.425
0.264CysThr: 0.264 ± 0.294
0.792CysVal: 0.792 ± 0.401
0.0CysTrp: 0.0 ± 0.0
0.792CysTyr: 0.792 ± 0.426
0.0CysXaa: 0.0 ± 0.0
Asp
6.073AspAla: 6.073 ± 1.196
0.528AspCys: 0.528 ± 0.438
5.281AspAsp: 5.281 ± 1.158
4.753AspGlu: 4.753 ± 0.877
2.641AspPhe: 2.641 ± 1.178
5.545AspGly: 5.545 ± 0.861
1.32AspHis: 1.32 ± 0.621
3.697AspIle: 3.697 ± 0.652
3.169AspLys: 3.169 ± 0.818
4.753AspLeu: 4.753 ± 1.173
2.377AspMet: 2.377 ± 0.57
1.32AspAsn: 1.32 ± 0.723
3.433AspPro: 3.433 ± 1.383
1.584AspGln: 1.584 ± 0.657
2.112AspArg: 2.112 ± 0.723
3.169AspSer: 3.169 ± 0.86
1.848AspThr: 1.848 ± 0.546
3.961AspVal: 3.961 ± 0.952
0.264AspTrp: 0.264 ± 0.294
1.32AspTyr: 1.32 ± 0.713
0.0AspXaa: 0.0 ± 0.0
Glu
5.545GluAla: 5.545 ± 1.191
0.0GluCys: 0.0 ± 0.0
3.961GluAsp: 3.961 ± 0.71
3.169GluGlu: 3.169 ± 1.04
2.641GluPhe: 2.641 ± 0.758
3.169GluGly: 3.169 ± 0.895
1.056GluHis: 1.056 ± 0.396
3.433GluIle: 3.433 ± 1.927
2.377GluLys: 2.377 ± 0.716
6.866GluLeu: 6.866 ± 1.971
1.056GluMet: 1.056 ± 0.423
2.112GluAsn: 2.112 ± 0.689
1.848GluPro: 1.848 ± 0.782
1.056GluGln: 1.056 ± 0.518
3.169GluArg: 3.169 ± 1.579
3.697GluSer: 3.697 ± 1.146
2.377GluThr: 2.377 ± 0.662
3.697GluVal: 3.697 ± 0.992
0.792GluTrp: 0.792 ± 0.396
1.056GluTyr: 1.056 ± 0.493
0.0GluXaa: 0.0 ± 0.0
Phe
3.961PheAla: 3.961 ± 1.172
0.0PheCys: 0.0 ± 0.0
3.169PheAsp: 3.169 ± 0.976
2.377PheGlu: 2.377 ± 0.858
2.112PhePhe: 2.112 ± 0.471
3.433PheGly: 3.433 ± 0.613
0.528PheHis: 0.528 ± 0.288
3.169PheIle: 3.169 ± 0.975
1.584PheLys: 1.584 ± 0.954
2.905PheLeu: 2.905 ± 1.021
1.056PheMet: 1.056 ± 0.41
2.377PheAsn: 2.377 ± 0.771
1.056PhePro: 1.056 ± 0.485
1.32PheGln: 1.32 ± 0.688
1.584PheArg: 1.584 ± 0.599
3.169PheSer: 3.169 ± 1.123
2.377PheThr: 2.377 ± 0.829
2.905PheVal: 2.905 ± 0.921
0.528PheTrp: 0.528 ± 0.315
0.792PheTyr: 0.792 ± 0.463
0.0PheXaa: 0.0 ± 0.0
Gly
7.13GlyAla: 7.13 ± 1.974
1.056GlyCys: 1.056 ± 0.651
5.809GlyAsp: 5.809 ± 0.894
5.809GlyGlu: 5.809 ± 2.158
3.433GlyPhe: 3.433 ± 1.008
5.809GlyGly: 5.809 ± 1.455
1.584GlyHis: 1.584 ± 0.642
2.905GlyIle: 2.905 ± 1.028
4.225GlyLys: 4.225 ± 1.113
6.866GlyLeu: 6.866 ± 1.589
2.377GlyMet: 2.377 ± 0.803
2.641GlyAsn: 2.641 ± 1.174
3.169GlyPro: 3.169 ± 1.563
2.112GlyGln: 2.112 ± 1.037
3.433GlyArg: 3.433 ± 1.423
5.281GlySer: 5.281 ± 0.968
4.489GlyThr: 4.489 ± 1.009
5.545GlyVal: 5.545 ± 1.497
1.056GlyTrp: 1.056 ± 0.516
4.489GlyTyr: 4.489 ± 0.874
0.0GlyXaa: 0.0 ± 0.0
His
1.848HisAla: 1.848 ± 0.962
0.0HisCys: 0.0 ± 0.0
0.528HisAsp: 0.528 ± 0.315
1.056HisGlu: 1.056 ± 0.437
0.264HisPhe: 0.264 ± 0.294
0.528HisGly: 0.528 ± 0.441
0.528HisHis: 0.528 ± 0.27
0.528HisIle: 0.528 ± 0.294
0.264HisLys: 0.264 ± 0.233
1.32HisLeu: 1.32 ± 0.404
0.528HisMet: 0.528 ± 0.405
0.528HisAsn: 0.528 ± 0.322
1.056HisPro: 1.056 ± 0.428
0.264HisGln: 0.264 ± 0.241
2.112HisArg: 2.112 ± 0.708
0.792HisSer: 0.792 ± 0.315
2.641HisThr: 2.641 ± 1.18
1.056HisVal: 1.056 ± 0.514
0.264HisTrp: 0.264 ± 0.3
1.056HisTyr: 1.056 ± 0.582
0.0HisXaa: 0.0 ± 0.0
Ile
6.337IleAla: 6.337 ± 1.212
0.528IleCys: 0.528 ± 0.27
2.377IleAsp: 2.377 ± 0.639
1.848IleGlu: 1.848 ± 0.683
1.584IlePhe: 1.584 ± 0.825
5.017IleGly: 5.017 ± 1.104
2.112IleHis: 2.112 ± 0.775
1.056IleIle: 1.056 ± 0.48
3.169IleLys: 3.169 ± 1.375
3.697IleLeu: 3.697 ± 1.075
1.584IleMet: 1.584 ± 0.805
2.905IleAsn: 2.905 ± 0.59
2.377IlePro: 2.377 ± 0.896
1.32IleGln: 1.32 ± 0.549
3.169IleArg: 3.169 ± 0.918
3.433IleSer: 3.433 ± 0.939
4.225IleThr: 4.225 ± 1.07
5.809IleVal: 5.809 ± 1.098
1.056IleTrp: 1.056 ± 0.532
0.792IleTyr: 0.792 ± 0.301
0.0IleXaa: 0.0 ± 0.0
Lys
5.281LysAla: 5.281 ± 1.604
0.792LysCys: 0.792 ± 0.81
2.905LysAsp: 2.905 ± 0.866
3.433LysGlu: 3.433 ± 0.994
1.32LysPhe: 1.32 ± 0.49
3.961LysGly: 3.961 ± 0.824
0.792LysHis: 0.792 ± 0.575
2.905LysIle: 2.905 ± 0.789
2.641LysLys: 2.641 ± 0.806
3.961LysLeu: 3.961 ± 0.44
1.848LysMet: 1.848 ± 0.738
2.377LysAsn: 2.377 ± 0.879
1.056LysPro: 1.056 ± 0.831
2.112LysGln: 2.112 ± 0.889
3.169LysArg: 3.169 ± 1.077
3.433LysSer: 3.433 ± 0.928
2.905LysThr: 2.905 ± 0.894
4.225LysVal: 4.225 ± 1.095
0.792LysTrp: 0.792 ± 0.419
1.32LysTyr: 1.32 ± 0.459
0.0LysXaa: 0.0 ± 0.0
Leu
8.45LeuAla: 8.45 ± 1.773
0.528LeuCys: 0.528 ± 0.431
3.961LeuAsp: 3.961 ± 0.893
4.489LeuGlu: 4.489 ± 1.26
4.225LeuPhe: 4.225 ± 1.087
6.337LeuGly: 6.337 ± 1.204
1.32LeuHis: 1.32 ± 0.453
4.225LeuIle: 4.225 ± 1.423
3.697LeuLys: 3.697 ± 0.604
6.866LeuLeu: 6.866 ± 1.705
2.641LeuMet: 2.641 ± 0.832
4.225LeuAsn: 4.225 ± 0.985
2.377LeuPro: 2.377 ± 0.819
5.281LeuGln: 5.281 ± 1.064
4.225LeuArg: 4.225 ± 0.966
6.073LeuSer: 6.073 ± 0.893
5.281LeuThr: 5.281 ± 1.569
7.658LeuVal: 7.658 ± 1.462
0.0LeuTrp: 0.0 ± 0.0
1.584LeuTyr: 1.584 ± 0.673
0.0LeuXaa: 0.0 ± 0.0
Met
2.905MetAla: 2.905 ± 0.904
0.0MetCys: 0.0 ± 0.0
2.641MetAsp: 2.641 ± 0.716
1.32MetGlu: 1.32 ± 0.721
1.32MetPhe: 1.32 ± 0.767
1.584MetGly: 1.584 ± 0.672
0.528MetHis: 0.528 ± 0.299
1.056MetIle: 1.056 ± 0.679
1.584MetLys: 1.584 ± 0.67
3.697MetLeu: 3.697 ± 1.365
1.848MetMet: 1.848 ± 0.88
1.584MetAsn: 1.584 ± 0.651
1.584MetPro: 1.584 ± 0.631
1.056MetGln: 1.056 ± 0.42
2.112MetArg: 2.112 ± 0.567
1.848MetSer: 1.848 ± 0.611
1.584MetThr: 1.584 ± 0.789
2.905MetVal: 2.905 ± 0.936
0.0MetTrp: 0.0 ± 0.0
0.792MetTyr: 0.792 ± 0.53
0.0MetXaa: 0.0 ± 0.0
Asn
3.697AsnAla: 3.697 ± 0.877
0.0AsnCys: 0.0 ± 0.0
2.905AsnAsp: 2.905 ± 0.687
1.32AsnGlu: 1.32 ± 0.741
1.32AsnPhe: 1.32 ± 0.804
3.169AsnGly: 3.169 ± 0.976
0.792AsnHis: 0.792 ± 0.565
1.848AsnIle: 1.848 ± 0.789
1.584AsnLys: 1.584 ± 0.575
1.056AsnLeu: 1.056 ± 0.462
2.112AsnMet: 2.112 ± 0.398
0.792AsnAsn: 0.792 ± 0.55
3.433AsnPro: 3.433 ± 0.866
0.792AsnGln: 0.792 ± 0.47
2.641AsnArg: 2.641 ± 0.825
3.697AsnSer: 3.697 ± 0.723
2.905AsnThr: 2.905 ± 0.805
5.809AsnVal: 5.809 ± 1.441
0.264AsnTrp: 0.264 ± 0.241
1.584AsnTyr: 1.584 ± 0.57
0.0AsnXaa: 0.0 ± 0.0
Pro
2.377ProAla: 2.377 ± 0.884
0.528ProCys: 0.528 ± 0.529
3.433ProAsp: 3.433 ± 1.278
2.112ProGlu: 2.112 ± 0.562
1.584ProPhe: 1.584 ± 0.908
3.169ProGly: 3.169 ± 0.846
0.264ProHis: 0.264 ± 0.233
1.848ProIle: 1.848 ± 0.692
1.848ProLys: 1.848 ± 0.741
4.753ProLeu: 4.753 ± 1.079
0.264ProMet: 0.264 ± 0.294
2.377ProAsn: 2.377 ± 0.788
1.056ProPro: 1.056 ± 0.336
2.112ProGln: 2.112 ± 0.722
2.905ProArg: 2.905 ± 0.818
2.641ProSer: 2.641 ± 0.824
2.905ProThr: 2.905 ± 0.779
2.377ProVal: 2.377 ± 0.707
0.264ProTrp: 0.264 ± 0.233
0.264ProTyr: 0.264 ± 0.313
0.0ProXaa: 0.0 ± 0.0
Gln
5.281GlnAla: 5.281 ± 1.349
0.264GlnCys: 0.264 ± 0.198
0.792GlnAsp: 0.792 ± 0.422
1.584GlnGlu: 1.584 ± 0.607
1.056GlnPhe: 1.056 ± 0.64
2.112GlnGly: 2.112 ± 1.104
0.792GlnHis: 0.792 ± 0.356
0.792GlnIle: 0.792 ± 0.371
1.848GlnLys: 1.848 ± 0.819
3.433GlnLeu: 3.433 ± 0.777
1.32GlnMet: 1.32 ± 0.456
0.792GlnAsn: 0.792 ± 0.383
1.848GlnPro: 1.848 ± 0.929
1.584GlnGln: 1.584 ± 0.664
2.641GlnArg: 2.641 ± 0.996
2.641GlnSer: 2.641 ± 1.009
1.848GlnThr: 1.848 ± 0.882
2.905GlnVal: 2.905 ± 0.914
0.792GlnTrp: 0.792 ± 0.301
1.584GlnTyr: 1.584 ± 0.441
0.0GlnXaa: 0.0 ± 0.0
Arg
5.281ArgAla: 5.281 ± 1.219
0.0ArgCys: 0.0 ± 0.0
2.377ArgAsp: 2.377 ± 1.081
2.112ArgGlu: 2.112 ± 0.865
2.905ArgPhe: 2.905 ± 0.741
2.641ArgGly: 2.641 ± 1.054
0.264ArgHis: 0.264 ± 0.264
2.905ArgIle: 2.905 ± 0.557
2.112ArgLys: 2.112 ± 0.974
3.697ArgLeu: 3.697 ± 0.846
2.905ArgMet: 2.905 ± 1.003
2.377ArgAsn: 2.377 ± 0.762
2.377ArgPro: 2.377 ± 0.63
3.169ArgGln: 3.169 ± 0.821
2.377ArgArg: 2.377 ± 0.868
3.961ArgSer: 3.961 ± 0.945
3.697ArgThr: 3.697 ± 1.145
4.489ArgVal: 4.489 ± 1.167
0.792ArgTrp: 0.792 ± 0.326
2.112ArgTyr: 2.112 ± 0.695
0.0ArgXaa: 0.0 ± 0.0
Ser
5.545SerAla: 5.545 ± 1.103
0.264SerCys: 0.264 ± 0.294
3.961SerAsp: 3.961 ± 0.879
2.377SerGlu: 2.377 ± 0.676
3.961SerPhe: 3.961 ± 0.916
7.658SerGly: 7.658 ± 1.869
1.32SerHis: 1.32 ± 0.662
2.641SerIle: 2.641 ± 0.537
4.753SerLys: 4.753 ± 1.073
7.13SerLeu: 7.13 ± 1.425
2.377SerMet: 2.377 ± 0.807
2.641SerAsn: 2.641 ± 0.827
2.377SerPro: 2.377 ± 0.957
0.264SerGln: 0.264 ± 0.294
3.169SerArg: 3.169 ± 1.031
2.905SerSer: 2.905 ± 0.762
5.017SerThr: 5.017 ± 1.497
7.922SerVal: 7.922 ± 1.465
1.056SerTrp: 1.056 ± 0.482
1.32SerTyr: 1.32 ± 0.632
0.0SerXaa: 0.0 ± 0.0
Thr
4.753ThrAla: 4.753 ± 1.406
0.792ThrCys: 0.792 ± 0.423
2.905ThrAsp: 2.905 ± 0.716
2.112ThrGlu: 2.112 ± 0.843
1.848ThrPhe: 1.848 ± 0.833
5.545ThrGly: 5.545 ± 1.02
0.792ThrHis: 0.792 ± 0.418
3.961ThrIle: 3.961 ± 1.194
2.112ThrLys: 2.112 ± 0.615
4.753ThrLeu: 4.753 ± 1.795
0.528ThrMet: 0.528 ± 0.351
1.848ThrAsn: 1.848 ± 0.443
1.848ThrPro: 1.848 ± 0.54
1.584ThrGln: 1.584 ± 0.612
3.169ThrArg: 3.169 ± 0.611
7.13ThrSer: 7.13 ± 1.925
5.545ThrThr: 5.545 ± 1.291
5.809ThrVal: 5.809 ± 1.004
0.792ThrTrp: 0.792 ± 0.55
1.584ThrTyr: 1.584 ± 0.612
0.0ThrXaa: 0.0 ± 0.0
Val
8.186ValAla: 8.186 ± 1.796
0.528ValCys: 0.528 ± 0.368
5.281ValAsp: 5.281 ± 0.985
4.225ValGlu: 4.225 ± 1.328
2.641ValPhe: 2.641 ± 0.675
6.866ValGly: 6.866 ± 0.794
1.584ValHis: 1.584 ± 0.713
6.602ValIle: 6.602 ± 2.057
6.602ValLys: 6.602 ± 1.996
7.13ValLeu: 7.13 ± 1.514
1.584ValMet: 1.584 ± 0.613
3.697ValAsn: 3.697 ± 0.647
2.641ValPro: 2.641 ± 0.962
2.641ValGln: 2.641 ± 0.525
2.905ValArg: 2.905 ± 0.946
5.545ValSer: 5.545 ± 1.216
4.753ValThr: 4.753 ± 1.156
8.45ValVal: 8.45 ± 2.188
1.584ValTrp: 1.584 ± 0.982
1.056ValTyr: 1.056 ± 0.512
0.0ValXaa: 0.0 ± 0.0
Trp
1.32TrpAla: 1.32 ± 0.537
0.0TrpCys: 0.0 ± 0.0
0.792TrpAsp: 0.792 ± 0.562
1.32TrpGlu: 1.32 ± 0.764
1.32TrpPhe: 1.32 ± 0.44
0.792TrpGly: 0.792 ± 0.429
0.264TrpHis: 0.264 ± 0.233
1.056TrpIle: 1.056 ± 0.614
1.32TrpLys: 1.32 ± 0.703
0.792TrpLeu: 0.792 ± 0.396
0.0TrpMet: 0.0 ± 0.0
0.792TrpAsn: 0.792 ± 0.711
0.792TrpPro: 0.792 ± 0.453
1.056TrpGln: 1.056 ± 0.38
0.528TrpArg: 0.528 ± 0.303
0.264TrpSer: 0.264 ± 0.241
0.528TrpThr: 0.528 ± 0.354
0.528TrpVal: 0.528 ± 0.434
0.0TrpTrp: 0.0 ± 0.0
0.0TrpTyr: 0.0 ± 0.0
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.697TyrAla: 3.697 ± 0.807
0.0TyrCys: 0.0 ± 0.0
2.377TyrAsp: 2.377 ± 1.042
1.584TyrGlu: 1.584 ± 0.44
0.528TyrPhe: 0.528 ± 0.34
2.905TyrGly: 2.905 ± 0.725
0.528TyrHis: 0.528 ± 0.303
1.32TyrIle: 1.32 ± 0.561
1.584TyrLys: 1.584 ± 0.551
2.377TyrLeu: 2.377 ± 0.605
0.528TyrMet: 0.528 ± 0.428
2.905TyrAsn: 2.905 ± 0.487
0.528TyrPro: 0.528 ± 0.315
0.792TyrGln: 0.792 ± 0.453
2.905TyrArg: 2.905 ± 0.869
0.528TyrSer: 0.528 ± 0.27
1.056TyrThr: 1.056 ± 0.503
1.056TyrVal: 1.056 ± 0.474
0.528TyrTrp: 0.528 ± 0.405
0.264TyrTyr: 0.264 ± 0.264
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 15 proteins (3788 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski