Amino acid dipepetide frequency for Thalassomonas phage BA3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.376AlaAla: 7.376 ± 1.227
0.405AlaCys: 0.405 ± 0.294
4.782AlaAsp: 4.782 ± 0.846
7.781AlaGlu: 7.781 ± 1.47
3.323AlaPhe: 3.323 ± 0.608
5.592AlaGly: 5.592 ± 0.719
0.973AlaHis: 0.973 ± 0.295
5.674AlaIle: 5.674 ± 0.65
7.7AlaLys: 7.7 ± 0.915
6.889AlaLeu: 6.889 ± 1.003
2.513AlaMet: 2.513 ± 0.371
4.944AlaAsn: 4.944 ± 0.572
2.35AlaPro: 2.35 ± 0.422
3.971AlaGln: 3.971 ± 0.981
3.08AlaArg: 3.08 ± 0.6
4.377AlaSer: 4.377 ± 0.772
4.458AlaThr: 4.458 ± 0.525
4.296AlaVal: 4.296 ± 0.733
0.729AlaTrp: 0.729 ± 0.251
2.513AlaTyr: 2.513 ± 0.607
0.0AlaXaa: 0.0 ± 0.0
Cys
0.811CysAla: 0.811 ± 0.322
0.243CysCys: 0.243 ± 0.197
0.648CysAsp: 0.648 ± 0.292
0.486CysGlu: 0.486 ± 0.192
0.486CysPhe: 0.486 ± 0.284
0.405CysGly: 0.405 ± 0.248
0.081CysHis: 0.081 ± 0.093
0.243CysIle: 0.243 ± 0.136
0.405CysLys: 0.405 ± 0.271
1.297CysLeu: 1.297 ± 0.494
0.081CysMet: 0.081 ± 0.082
0.405CysAsn: 0.405 ± 0.252
0.162CysPro: 0.162 ± 0.142
0.243CysGln: 0.243 ± 0.177
0.243CysArg: 0.243 ± 0.198
0.648CysSer: 0.648 ± 0.298
0.162CysThr: 0.162 ± 0.116
0.486CysVal: 0.486 ± 0.243
0.162CysTrp: 0.162 ± 0.116
0.648CysTyr: 0.648 ± 0.295
0.0CysXaa: 0.0 ± 0.0
Asp
3.323AspAla: 3.323 ± 0.55
0.567AspCys: 0.567 ± 0.244
3.485AspAsp: 3.485 ± 0.595
4.377AspGlu: 4.377 ± 0.737
2.756AspPhe: 2.756 ± 0.478
3.728AspGly: 3.728 ± 0.639
1.378AspHis: 1.378 ± 0.374
3.647AspIle: 3.647 ± 0.499
4.944AspLys: 4.944 ± 0.877
5.025AspLeu: 5.025 ± 0.888
1.783AspMet: 1.783 ± 0.359
3.08AspAsn: 3.08 ± 0.509
3.161AspPro: 3.161 ± 0.571
2.999AspGln: 2.999 ± 0.443
2.756AspArg: 2.756 ± 0.48
2.999AspSer: 2.999 ± 0.603
3.647AspThr: 3.647 ± 0.71
2.837AspVal: 2.837 ± 0.425
0.243AspTrp: 0.243 ± 0.131
1.702AspTyr: 1.702 ± 0.537
0.0AspXaa: 0.0 ± 0.0
Glu
8.429GluAla: 8.429 ± 1.369
0.648GluCys: 0.648 ± 0.24
4.296GluAsp: 4.296 ± 0.921
6.484GluGlu: 6.484 ± 1.072
3.89GluPhe: 3.89 ± 0.587
4.944GluGly: 4.944 ± 0.61
1.378GluHis: 1.378 ± 0.429
5.592GluIle: 5.592 ± 0.807
5.998GluLys: 5.998 ± 0.976
7.132GluLeu: 7.132 ± 0.879
1.864GluMet: 1.864 ± 0.442
2.756GluAsn: 2.756 ± 0.622
2.269GluPro: 2.269 ± 0.503
5.349GluGln: 5.349 ± 0.97
4.701GluArg: 4.701 ± 0.667
4.539GluSer: 4.539 ± 0.635
4.053GluThr: 4.053 ± 0.742
5.43GluVal: 5.43 ± 0.963
1.54GluTrp: 1.54 ± 0.322
2.269GluTyr: 2.269 ± 0.322
0.0GluXaa: 0.0 ± 0.0
Phe
2.675PheAla: 2.675 ± 0.481
0.324PheCys: 0.324 ± 0.195
2.756PheAsp: 2.756 ± 0.61
2.999PheGlu: 2.999 ± 0.617
1.216PhePhe: 1.216 ± 0.335
2.675PheGly: 2.675 ± 0.52
0.892PheHis: 0.892 ± 0.348
2.999PheIle: 2.999 ± 0.519
3.485PheLys: 3.485 ± 0.58
2.35PheLeu: 2.35 ± 0.419
0.729PheMet: 0.729 ± 0.206
2.432PheAsn: 2.432 ± 0.456
0.892PhePro: 0.892 ± 0.224
1.864PheGln: 1.864 ± 0.322
1.945PheArg: 1.945 ± 0.561
2.188PheSer: 2.188 ± 0.437
2.513PheThr: 2.513 ± 0.416
2.107PheVal: 2.107 ± 0.547
0.405PheTrp: 0.405 ± 0.182
1.702PheTyr: 1.702 ± 0.389
0.0PheXaa: 0.0 ± 0.0
Gly
5.187GlyAla: 5.187 ± 0.881
0.567GlyCys: 0.567 ± 0.28
4.377GlyAsp: 4.377 ± 0.615
4.377GlyGlu: 4.377 ± 0.447
3.242GlyPhe: 3.242 ± 0.565
5.511GlyGly: 5.511 ± 0.929
0.892GlyHis: 0.892 ± 0.25
4.701GlyIle: 4.701 ± 0.797
6.16GlyLys: 6.16 ± 0.755
3.404GlyLeu: 3.404 ± 0.564
1.702GlyMet: 1.702 ± 0.35
2.918GlyAsn: 2.918 ± 0.623
1.054GlyPro: 1.054 ± 0.257
2.594GlyGln: 2.594 ± 0.705
3.485GlyArg: 3.485 ± 0.584
4.134GlySer: 4.134 ± 1.019
3.971GlyThr: 3.971 ± 0.596
4.863GlyVal: 4.863 ± 0.644
1.054GlyTrp: 1.054 ± 0.341
3.08GlyTyr: 3.08 ± 0.512
0.0GlyXaa: 0.0 ± 0.0
His
0.892HisAla: 0.892 ± 0.237
0.162HisCys: 0.162 ± 0.121
1.054HisAsp: 1.054 ± 0.383
1.378HisGlu: 1.378 ± 0.438
0.324HisPhe: 0.324 ± 0.181
1.216HisGly: 1.216 ± 0.358
0.243HisHis: 0.243 ± 0.18
0.729HisIle: 0.729 ± 0.239
1.135HisLys: 1.135 ± 0.433
1.54HisLeu: 1.54 ± 0.437
0.486HisMet: 0.486 ± 0.182
0.892HisAsn: 0.892 ± 0.252
0.162HisPro: 0.162 ± 0.134
0.567HisGln: 0.567 ± 0.33
1.054HisArg: 1.054 ± 0.316
0.811HisSer: 0.811 ± 0.284
0.567HisThr: 0.567 ± 0.175
0.811HisVal: 0.811 ± 0.254
0.162HisTrp: 0.162 ± 0.123
0.729HisTyr: 0.729 ± 0.265
0.0HisXaa: 0.0 ± 0.0
Ile
5.187IleAla: 5.187 ± 0.788
0.729IleCys: 0.729 ± 0.3
4.377IleAsp: 4.377 ± 0.68
5.917IleGlu: 5.917 ± 0.789
1.459IlePhe: 1.459 ± 0.271
3.971IleGly: 3.971 ± 0.738
0.892IleHis: 0.892 ± 0.335
2.918IleIle: 2.918 ± 0.557
5.025IleLys: 5.025 ± 0.682
3.647IleLeu: 3.647 ± 0.627
1.621IleMet: 1.621 ± 0.365
3.647IleAsn: 3.647 ± 0.675
2.756IlePro: 2.756 ± 0.62
2.269IleGln: 2.269 ± 0.44
2.675IleArg: 2.675 ± 0.355
3.809IleSer: 3.809 ± 0.682
4.782IleThr: 4.782 ± 0.883
2.999IleVal: 2.999 ± 0.619
0.405IleTrp: 0.405 ± 0.221
2.026IleTyr: 2.026 ± 0.573
0.0IleXaa: 0.0 ± 0.0
Lys
8.834LysAla: 8.834 ± 1.349
0.567LysCys: 0.567 ± 0.248
4.539LysAsp: 4.539 ± 0.901
6.079LysGlu: 6.079 ± 0.826
2.918LysPhe: 2.918 ± 0.453
5.268LysGly: 5.268 ± 0.729
1.378LysHis: 1.378 ± 0.329
3.728LysIle: 3.728 ± 0.588
6.727LysLys: 6.727 ± 0.885
6.808LysLeu: 6.808 ± 0.929
2.107LysMet: 2.107 ± 0.401
3.485LysAsn: 3.485 ± 0.503
3.728LysPro: 3.728 ± 1.071
4.377LysGln: 4.377 ± 0.629
3.647LysArg: 3.647 ± 0.557
5.106LysSer: 5.106 ± 0.632
4.215LysThr: 4.215 ± 0.428
4.377LysVal: 4.377 ± 0.487
0.648LysTrp: 0.648 ± 0.26
2.432LysTyr: 2.432 ± 0.446
0.0LysXaa: 0.0 ± 0.0
Leu
7.213LeuAla: 7.213 ± 1.068
0.486LeuCys: 0.486 ± 0.272
3.89LeuAsp: 3.89 ± 0.646
7.376LeuGlu: 7.376 ± 0.794
2.432LeuPhe: 2.432 ± 0.459
4.539LeuGly: 4.539 ± 0.525
1.216LeuHis: 1.216 ± 0.461
5.025LeuIle: 5.025 ± 0.829
6.322LeuLys: 6.322 ± 1.0
7.376LeuLeu: 7.376 ± 1.652
1.459LeuMet: 1.459 ± 0.295
3.89LeuAsn: 3.89 ± 0.676
3.242LeuPro: 3.242 ± 0.565
2.918LeuGln: 2.918 ± 0.477
3.404LeuArg: 3.404 ± 0.571
5.917LeuSer: 5.917 ± 0.749
5.998LeuThr: 5.998 ± 0.862
4.296LeuVal: 4.296 ± 0.732
1.54LeuTrp: 1.54 ± 0.369
1.702LeuTyr: 1.702 ± 0.481
0.0LeuXaa: 0.0 ± 0.0
Met
3.485MetAla: 3.485 ± 0.739
0.0MetCys: 0.0 ± 0.0
1.297MetAsp: 1.297 ± 0.283
1.621MetGlu: 1.621 ± 0.415
0.973MetPhe: 0.973 ± 0.306
1.459MetGly: 1.459 ± 0.373
0.243MetHis: 0.243 ± 0.127
1.864MetIle: 1.864 ± 0.392
2.35MetLys: 2.35 ± 0.447
2.35MetLeu: 2.35 ± 0.477
0.486MetMet: 0.486 ± 0.17
0.892MetAsn: 0.892 ± 0.334
1.297MetPro: 1.297 ± 0.333
0.486MetGln: 0.486 ± 0.154
0.892MetArg: 0.892 ± 0.288
1.54MetSer: 1.54 ± 0.439
0.973MetThr: 0.973 ± 0.301
1.297MetVal: 1.297 ± 0.353
0.0MetTrp: 0.0 ± 0.0
0.729MetTyr: 0.729 ± 0.209
0.0MetXaa: 0.0 ± 0.0
Asn
2.675AsnAla: 2.675 ± 0.533
0.648AsnCys: 0.648 ± 0.286
2.756AsnAsp: 2.756 ± 0.475
3.404AsnGlu: 3.404 ± 0.5
1.945AsnPhe: 1.945 ± 0.461
4.053AsnGly: 4.053 ± 1.007
0.973AsnHis: 0.973 ± 0.388
3.404AsnIle: 3.404 ± 0.679
3.971AsnLys: 3.971 ± 0.624
4.296AsnLeu: 4.296 ± 0.74
1.297AsnMet: 1.297 ± 0.338
3.728AsnAsn: 3.728 ± 0.797
2.756AsnPro: 2.756 ± 0.524
2.594AsnGln: 2.594 ± 0.487
2.513AsnArg: 2.513 ± 0.5
2.675AsnSer: 2.675 ± 0.623
1.945AsnThr: 1.945 ± 0.402
2.918AsnVal: 2.918 ± 0.455
1.135AsnTrp: 1.135 ± 0.368
1.702AsnTyr: 1.702 ± 0.361
0.0AsnXaa: 0.0 ± 0.0
Pro
2.675ProAla: 2.675 ± 0.523
0.243ProCys: 0.243 ± 0.164
2.35ProAsp: 2.35 ± 0.636
5.674ProGlu: 5.674 ± 1.092
1.54ProPhe: 1.54 ± 0.419
1.783ProGly: 1.783 ± 0.362
0.486ProHis: 0.486 ± 0.263
1.621ProIle: 1.621 ± 0.354
2.837ProLys: 2.837 ± 0.521
3.323ProLeu: 3.323 ± 0.698
0.567ProMet: 0.567 ± 0.187
1.216ProAsn: 1.216 ± 0.329
0.892ProPro: 0.892 ± 0.303
1.297ProGln: 1.297 ± 0.341
1.054ProArg: 1.054 ± 0.342
2.107ProSer: 2.107 ± 0.519
2.513ProThr: 2.513 ± 0.43
3.728ProVal: 3.728 ± 0.585
0.162ProTrp: 0.162 ± 0.13
0.648ProTyr: 0.648 ± 0.228
0.0ProXaa: 0.0 ± 0.0
Gln
5.106GlnAla: 5.106 ± 0.822
0.162GlnCys: 0.162 ± 0.128
1.945GlnAsp: 1.945 ± 0.332
4.539GlnGlu: 4.539 ± 0.561
2.026GlnPhe: 2.026 ± 0.466
3.242GlnGly: 3.242 ± 0.577
0.405GlnHis: 0.405 ± 0.193
2.188GlnIle: 2.188 ± 0.371
2.837GlnLys: 2.837 ± 0.601
4.377GlnLeu: 4.377 ± 0.76
1.297GlnMet: 1.297 ± 0.266
2.594GlnAsn: 2.594 ± 0.7
1.621GlnPro: 1.621 ± 0.357
3.89GlnGln: 3.89 ± 1.431
2.675GlnArg: 2.675 ± 0.516
2.269GlnSer: 2.269 ± 0.543
2.188GlnThr: 2.188 ± 0.47
2.35GlnVal: 2.35 ± 0.438
0.405GlnTrp: 0.405 ± 0.16
1.054GlnTyr: 1.054 ± 0.242
0.0GlnXaa: 0.0 ± 0.0
Arg
4.458ArgAla: 4.458 ± 0.692
0.405ArgCys: 0.405 ± 0.255
2.188ArgAsp: 2.188 ± 0.381
3.89ArgGlu: 3.89 ± 0.66
1.783ArgPhe: 1.783 ± 0.371
1.864ArgGly: 1.864 ± 0.464
0.729ArgHis: 0.729 ± 0.282
3.404ArgIle: 3.404 ± 0.44
5.025ArgLys: 5.025 ± 0.737
3.89ArgLeu: 3.89 ± 0.613
1.216ArgMet: 1.216 ± 0.271
2.756ArgAsn: 2.756 ± 0.404
2.107ArgPro: 2.107 ± 0.432
2.026ArgGln: 2.026 ± 0.473
2.837ArgArg: 2.837 ± 0.55
2.432ArgSer: 2.432 ± 0.497
1.702ArgThr: 1.702 ± 0.446
3.323ArgVal: 3.323 ± 0.527
0.243ArgTrp: 0.243 ± 0.161
1.054ArgTyr: 1.054 ± 0.275
0.0ArgXaa: 0.0 ± 0.0
Ser
4.134SerAla: 4.134 ± 0.648
0.324SerCys: 0.324 ± 0.184
3.971SerAsp: 3.971 ± 0.665
4.296SerGlu: 4.296 ± 0.555
2.999SerPhe: 2.999 ± 0.527
5.592SerGly: 5.592 ± 1.024
0.324SerHis: 0.324 ± 0.222
3.485SerIle: 3.485 ± 0.573
4.944SerLys: 4.944 ± 0.734
4.944SerLeu: 4.944 ± 0.823
1.54SerMet: 1.54 ± 0.348
3.566SerAsn: 3.566 ± 0.787
1.783SerPro: 1.783 ± 0.383
2.594SerGln: 2.594 ± 0.518
2.675SerArg: 2.675 ± 0.605
4.458SerSer: 4.458 ± 1.498
2.837SerThr: 2.837 ± 0.505
3.242SerVal: 3.242 ± 0.729
0.324SerTrp: 0.324 ± 0.194
2.026SerTyr: 2.026 ± 0.482
0.0SerXaa: 0.0 ± 0.0
Thr
4.134ThrAla: 4.134 ± 0.596
0.892ThrCys: 0.892 ± 0.36
2.918ThrAsp: 2.918 ± 0.453
4.134ThrGlu: 4.134 ± 0.984
2.107ThrPhe: 2.107 ± 0.554
5.592ThrGly: 5.592 ± 0.511
0.729ThrHis: 0.729 ± 0.274
3.647ThrIle: 3.647 ± 0.454
3.647ThrLys: 3.647 ± 0.562
4.134ThrLeu: 4.134 ± 0.665
0.811ThrMet: 0.811 ± 0.266
2.999ThrAsn: 2.999 ± 0.706
3.323ThrPro: 3.323 ± 0.59
2.107ThrGln: 2.107 ± 0.535
2.026ThrArg: 2.026 ± 0.47
3.161ThrSer: 3.161 ± 0.491
2.837ThrThr: 2.837 ± 0.486
3.161ThrVal: 3.161 ± 0.642
0.324ThrTrp: 0.324 ± 0.187
2.269ThrTyr: 2.269 ± 0.555
0.0ThrXaa: 0.0 ± 0.0
Val
4.377ValAla: 4.377 ± 0.557
0.243ValCys: 0.243 ± 0.195
3.647ValAsp: 3.647 ± 0.525
5.025ValGlu: 5.025 ± 0.637
2.107ValPhe: 2.107 ± 0.377
3.485ValGly: 3.485 ± 0.794
0.243ValHis: 0.243 ± 0.141
3.728ValIle: 3.728 ± 0.626
4.782ValLys: 4.782 ± 0.855
3.809ValLeu: 3.809 ± 0.55
1.945ValMet: 1.945 ± 0.425
2.999ValAsn: 2.999 ± 0.672
1.945ValPro: 1.945 ± 0.36
2.35ValGln: 2.35 ± 0.401
3.161ValArg: 3.161 ± 0.62
3.89ValSer: 3.89 ± 0.511
3.647ValThr: 3.647 ± 0.517
3.323ValVal: 3.323 ± 0.528
0.811ValTrp: 0.811 ± 0.325
1.864ValTyr: 1.864 ± 0.441
0.0ValXaa: 0.0 ± 0.0
Trp
0.892TrpAla: 0.892 ± 0.258
0.486TrpCys: 0.486 ± 0.242
0.811TrpAsp: 0.811 ± 0.235
0.811TrpGlu: 0.811 ± 0.228
0.486TrpPhe: 0.486 ± 0.201
0.892TrpGly: 0.892 ± 0.321
0.729TrpHis: 0.729 ± 0.242
0.567TrpIle: 0.567 ± 0.229
0.648TrpLys: 0.648 ± 0.233
1.54TrpLeu: 1.54 ± 0.453
0.081TrpMet: 0.081 ± 0.094
0.648TrpAsn: 0.648 ± 0.28
0.486TrpPro: 0.486 ± 0.249
0.324TrpGln: 0.324 ± 0.139
0.567TrpArg: 0.567 ± 0.292
0.405TrpSer: 0.405 ± 0.252
0.324TrpThr: 0.324 ± 0.152
0.324TrpVal: 0.324 ± 0.183
0.162TrpTrp: 0.162 ± 0.105
0.162TrpTyr: 0.162 ± 0.13
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.864TyrAla: 1.864 ± 0.454
0.243TyrCys: 0.243 ± 0.155
2.35TyrAsp: 2.35 ± 0.453
2.918TyrGlu: 2.918 ± 0.649
1.135TyrPhe: 1.135 ± 0.425
1.459TyrGly: 1.459 ± 0.339
0.729TyrHis: 0.729 ± 0.322
1.945TyrIle: 1.945 ± 0.408
2.026TyrLys: 2.026 ± 0.349
2.107TyrLeu: 2.107 ± 0.368
0.567TyrMet: 0.567 ± 0.303
1.54TyrAsn: 1.54 ± 0.307
0.729TyrPro: 0.729 ± 0.325
2.35TyrGln: 2.35 ± 0.511
1.945TyrArg: 1.945 ± 0.405
2.594TyrSer: 2.594 ± 0.426
1.621TyrThr: 1.621 ± 0.383
1.216TyrVal: 1.216 ± 0.311
0.892TyrTrp: 0.892 ± 0.332
1.459TyrTyr: 1.459 ± 0.396
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 47 proteins (12339 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski