[Clostridium] innocuum 2959

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Erysipelotrichia; Erysipelotrichales; Erysipelotrichaceae; Erysipelatoclostridium; [Clostridium] innocuum

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4653 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|N9WUR9|N9WUR9_CLOIN HTH deoR-type domain-containing protein OS=[Clostridium] innocuum 2959 OX=999413 GN=HMPREF1094_02081 PE=4 SV=1
MM1 pKa = 7.66HH2 pKa = 7.31YY3 pKa = 10.74DD4 pKa = 5.22DD5 pKa = 6.35YY6 pKa = 12.1DD7 pKa = 4.83FDD9 pKa = 6.32DD10 pKa = 4.77KK11 pKa = 11.75NCCNEE16 pKa = 4.3CSSSSCDD23 pKa = 3.13QGDD26 pKa = 3.86YY27 pKa = 10.96DD28 pKa = 4.41VCCCPGPRR36 pKa = 11.84GPRR39 pKa = 11.84GFRR42 pKa = 11.84GPAGEE47 pKa = 4.6AGATGATGATGNTGLTGATGATGLSEE73 pKa = 4.58TITIRR78 pKa = 11.84STTTADD84 pKa = 3.44PGTPAQVHH92 pKa = 6.7DD93 pKa = 4.31SGGPNHH99 pKa = 6.2VLDD102 pKa = 4.39FVIPRR107 pKa = 11.84GDD109 pKa = 3.37TGVTGATGPTGPGVGATGPTGATGATGNTGATGPIGATGPTGANGNTGPTGAIGATGEE167 pKa = 4.14NGATGPTGPAGPTGATGSTGAAGATGVTGPTGDD200 pKa = 3.82IGPTGPTGATGSTGAAGATGVTGATGDD227 pKa = 3.57IGPTGPTGATGPTGATGNTGATGTTGVTGADD258 pKa = 3.76GAIGPTGPIGDD269 pKa = 5.16PGPQGEE275 pKa = 5.01PGPQGEE281 pKa = 4.92PGPQGEE287 pKa = 4.45QGPQGEE293 pKa = 4.62TGATPIVRR301 pKa = 11.84VGSTTTSEE309 pKa = 4.34PGTPADD315 pKa = 3.32VSYY318 pKa = 11.62VEE320 pKa = 4.37TPDD323 pKa = 4.73GIEE326 pKa = 4.17LNFIIPRR333 pKa = 11.84GDD335 pKa = 3.22TGPGGGGGGLLAYY348 pKa = 9.67GGKK351 pKa = 10.59YY352 pKa = 9.69NDD354 pKa = 3.8SAQTLNLLIGAEE366 pKa = 4.09QQLPLPVDD374 pKa = 3.81MSASNVDD381 pKa = 4.21LAPVNALTIVTSGVYY396 pKa = 9.83EE397 pKa = 3.84INYY400 pKa = 7.27MFNASASLGAAVTLAVRR417 pKa = 11.84RR418 pKa = 11.84NGAAIPSTEE427 pKa = 3.91EE428 pKa = 3.44RR429 pKa = 11.84HH430 pKa = 5.85LLAIATEE437 pKa = 4.59SIYY440 pKa = 10.68SGSVIEE446 pKa = 4.77PLSAGDD452 pKa = 4.17IIDD455 pKa = 3.87MAVSAAIALTLTLSTGVTVTLSVKK479 pKa = 10.35RR480 pKa = 11.84LNDD483 pKa = 3.35ALANN487 pKa = 3.9

Molecular weight: 46.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|N9VCF8|N9VCF8_CLOIN Peptide chain release factor 1 OS=[Clostridium] innocuum 2959 OX=999413 GN=prfA PE=3 SV=1
MM1 pKa = 7.41FFHH4 pKa = 6.36SWRR7 pKa = 11.84RR8 pKa = 11.84KK9 pKa = 8.67RR10 pKa = 11.84KK11 pKa = 8.75LKK13 pKa = 10.22RR14 pKa = 11.84FRR16 pKa = 11.84PIMTLHH22 pKa = 7.11LNRR25 pKa = 11.84TLHH28 pKa = 6.71HH29 pKa = 6.42EE30 pKa = 4.2FHH32 pKa = 6.56PTFLKK37 pKa = 10.84AKK39 pKa = 8.0TVLRR43 pKa = 11.84EE44 pKa = 3.85VRR46 pKa = 11.84SSVIRR51 pKa = 11.84KK52 pKa = 9.06EE53 pKa = 3.92EE54 pKa = 4.03HH55 pKa = 4.86THH57 pKa = 6.6HH58 pKa = 6.43IHH60 pKa = 7.73HH61 pKa = 6.71MMTLHH66 pKa = 5.69VHH68 pKa = 5.11QAQTFLMLNHH78 pKa = 6.12QNDD81 pKa = 3.69EE82 pKa = 4.08RR83 pKa = 11.84SLIIQSKK90 pKa = 9.78KK91 pKa = 10.05VINRR95 pKa = 11.84KK96 pKa = 8.59YY97 pKa = 9.95PSRR100 pKa = 11.84ILQLHH105 pKa = 6.41LAKK108 pKa = 10.53QSIEE112 pKa = 3.89RR113 pKa = 11.84LAEE116 pKa = 3.77QRR118 pKa = 11.84LLEE121 pKa = 4.3EE122 pKa = 3.72KK123 pKa = 10.25SYY125 pKa = 11.54YY126 pKa = 10.01KK127 pKa = 9.88YY128 pKa = 10.68HH129 pKa = 6.49PAKK132 pKa = 10.44AKK134 pKa = 9.84EE135 pKa = 3.81LAVSYY140 pKa = 9.22LHH142 pKa = 6.56NRR144 pKa = 11.84EE145 pKa = 4.17IKK147 pKa = 10.27NCLNTHH153 pKa = 5.9MNRR156 pKa = 11.84LSQYY160 pKa = 10.07SYY162 pKa = 10.89AGSLFRR168 pKa = 11.84SSLFTTLSSQSWKK181 pKa = 10.11QVLIPVIYY189 pKa = 8.79QQSRR193 pKa = 11.84ALIHH197 pKa = 6.43RR198 pKa = 11.84EE199 pKa = 3.63KK200 pKa = 10.52IQLQRR205 pKa = 11.84EE206 pKa = 4.52VISEE210 pKa = 4.02KK211 pKa = 10.83SRR213 pKa = 11.84DD214 pKa = 3.59EE215 pKa = 3.79MHH217 pKa = 6.56VEE219 pKa = 4.06RR220 pKa = 11.84EE221 pKa = 4.22PEE223 pKa = 3.52HH224 pKa = 6.41RR225 pKa = 11.84AAVQRR230 pKa = 11.84NDD232 pKa = 2.96MNTLVNTVIRR242 pKa = 11.84EE243 pKa = 3.9VEE245 pKa = 3.8RR246 pKa = 11.84RR247 pKa = 11.84TKK249 pKa = 10.42RR250 pKa = 11.84EE251 pKa = 3.31RR252 pKa = 11.84LRR254 pKa = 11.84EE255 pKa = 3.98GRR257 pKa = 11.84RR258 pKa = 3.32

Molecular weight: 31.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4653

0

4653

1343130

29

2670

288.7

32.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.181 ± 0.037

1.601 ± 0.014

5.703 ± 0.032

7.141 ± 0.041

4.197 ± 0.029

6.175 ± 0.043

2.278 ± 0.019

7.611 ± 0.034

6.825 ± 0.037

9.692 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.324 ± 0.019

4.227 ± 0.023

3.019 ± 0.019

3.958 ± 0.029

4.497 ± 0.028

5.796 ± 0.025

5.174 ± 0.03

6.427 ± 0.032

0.882 ± 0.011

4.292 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski