Amino acid dipepetide frequency for Firmicutes bacterium CAG:240

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	11.605AlaAla: 11.605 ± 0.199	1.652AlaCys: 1.652 ± 0.063	5.975AlaAsp: 5.975 ± 0.126	7.069AlaGlu: 7.069 ± 0.141	3.657AlaPhe: 3.657 ± 0.098	6.77AlaGly: 6.77 ± 0.124	1.454AlaHis: 1.454 ± 0.053	5.764AlaIle: 5.764 ± 0.114	5.152AlaLys: 5.152 ± 0.101	9.466AlaLeu: 9.466 ± 0.148	2.958AlaMet: 2.958 ± 0.075	2.723AlaAsn: 2.723 ± 0.071	2.92AlaPro: 2.92 ± 0.074	3.116AlaGln: 3.116 ± 0.082	4.004AlaArg: 4.004 ± 0.087	4.871AlaSer: 4.871 ± 0.088	3.45AlaThr: 3.45 ± 0.087	8.193AlaVal: 8.193 ± 0.128	0.687AlaTrp: 0.687 ± 0.038	2.927AlaTyr: 2.927 ± 0.082	0.004AlaXaa: 0.004 ± 0.002
Cys	2.014CysAla: 2.014 ± 0.065	0.433CysCys: 0.433 ± 0.029	1.284CysAsp: 1.284 ± 0.053	1.053CysGlu: 1.053 ± 0.046	0.842CysPhe: 0.842 ± 0.044	2.128CysGly: 2.128 ± 0.065	0.298CysHis: 0.298 ± 0.025	1.275CysIle: 1.275 ± 0.053	0.873CysLys: 0.873 ± 0.039	1.473CysLeu: 1.473 ± 0.048	0.454CysMet: 0.454 ± 0.03	0.55CysAsn: 0.55 ± 0.034	0.849CysPro: 0.849 ± 0.045	0.337CysGln: 0.337 ± 0.026	1.167CysArg: 1.167 ± 0.05	1.219CysSer: 1.219 ± 0.048	1.08CysThr: 1.08 ± 0.056	1.417CysVal: 1.417 ± 0.058	0.18CysTrp: 0.18 ± 0.019	0.619CysTyr: 0.619 ± 0.04	0.0CysXaa: 0.0 ± 0.0
Asp	5.573AspAla: 5.573 ± 0.111	1.102AspCys: 1.102 ± 0.045	3.452AspAsp: 3.452 ± 0.091	4.75AspGlu: 4.75 ± 0.109	2.395AspPhe: 2.395 ± 0.075	5.131AspGly: 5.131 ± 0.103	0.878AspHis: 0.878 ± 0.041	4.568AspIle: 4.568 ± 0.101	3.708AspLys: 3.708 ± 0.084	4.276AspLeu: 4.276 ± 0.102	2.038AspMet: 2.038 ± 0.063	2.139AspAsn: 2.139 ± 0.06	2.247AspPro: 2.247 ± 0.063	0.927AspGln: 0.927 ± 0.036	2.541AspArg: 2.541 ± 0.074	3.095AspSer: 3.095 ± 0.075	3.028AspThr: 3.028 ± 0.073	4.031AspVal: 4.031 ± 0.094	0.572AspTrp: 0.572 ± 0.034	2.523AspTyr: 2.523 ± 0.077	0.0AspXaa: 0.0 ± 0.0
Glu	5.495GluAla: 5.495 ± 0.104	0.994GluCys: 0.994 ± 0.045	3.383GluAsp: 3.383 ± 0.079	4.171GluGlu: 4.171 ± 0.11	2.35GluPhe: 2.35 ± 0.078	3.969GluGly: 3.969 ± 0.097	1.432GluHis: 1.432 ± 0.054	4.415GluIle: 4.415 ± 0.101	5.235GluLys: 5.235 ± 0.095	7.376GluLeu: 7.376 ± 0.134	2.114GluMet: 2.114 ± 0.059	3.318GluAsn: 3.318 ± 0.08	2.153GluPro: 2.153 ± 0.069	2.335GluGln: 2.335 ± 0.071	3.661GluArg: 3.661 ± 0.092	3.279GluSer: 3.279 ± 0.072	3.221GluThr: 3.221 ± 0.074	3.865GluVal: 3.865 ± 0.092	0.492GluTrp: 0.492 ± 0.029	2.757GluTyr: 2.757 ± 0.077	0.002GluXaa: 0.002 ± 0.002
Phe	3.96PheAla: 3.96 ± 0.094	0.855PheCys: 0.855 ± 0.041	2.756PheAsp: 2.756 ± 0.073	2.559PheGlu: 2.559 ± 0.07	1.742PhePhe: 1.742 ± 0.069	3.511PheGly: 3.511 ± 0.083	0.541PheHis: 0.541 ± 0.031	2.647PheIle: 2.647 ± 0.08	2.049PheLys: 2.049 ± 0.065	3.284PheLeu: 3.284 ± 0.077	1.165PheMet: 1.165 ± 0.046	1.329PheAsn: 1.329 ± 0.045	1.369PhePro: 1.369 ± 0.053	0.671PheGln: 0.671 ± 0.032	1.654PheArg: 1.654 ± 0.053	2.95PheSer: 2.95 ± 0.076	2.343PheThr: 2.343 ± 0.071	3.004PheVal: 3.004 ± 0.075	0.359PheTrp: 0.359 ± 0.026	1.425PheTyr: 1.425 ± 0.051	0.0PheXaa: 0.0 ± 0.0
Gly	6.667GlyAla: 6.667 ± 0.122	1.627GlyCys: 1.627 ± 0.064	4.287GlyAsp: 4.287 ± 0.095	4.952GlyGlu: 4.952 ± 0.102	3.304GlyPhe: 3.304 ± 0.088	5.809GlyGly: 5.809 ± 0.124	1.306GlyHis: 1.306 ± 0.046	5.852GlyIle: 5.852 ± 0.105	5.21GlyLys: 5.21 ± 0.1	6.418GlyLeu: 6.418 ± 0.11	2.541GlyMet: 2.541 ± 0.076	2.595GlyAsn: 2.595 ± 0.07	1.316GlyPro: 1.316 ± 0.047	1.944GlyGln: 1.944 ± 0.064	3.657GlyArg: 3.657 ± 0.089	5.023GlySer: 5.023 ± 0.107	4.316GlyThr: 4.316 ± 0.103	6.04GlyVal: 6.04 ± 0.111	0.756GlyTrp: 0.756 ± 0.042	3.039GlyTyr: 3.039 ± 0.073	0.005GlyXaa: 0.005 ± 0.003
His	1.347HisAla: 1.347 ± 0.048	0.357HisCys: 0.357 ± 0.027	1.089HisAsp: 1.089 ± 0.047	1.066HisGlu: 1.066 ± 0.044	0.718HisPhe: 0.718 ± 0.035	1.472HisGly: 1.472 ± 0.053	0.35HisHis: 0.35 ± 0.028	1.199HisIle: 1.199 ± 0.046	0.857HisLys: 0.857 ± 0.042	1.403HisLeu: 1.403 ± 0.053	0.516HisMet: 0.516 ± 0.03	0.642HisAsn: 0.642 ± 0.034	0.851HisPro: 0.851 ± 0.043	0.348HisGln: 0.348 ± 0.026	0.963HisArg: 0.963 ± 0.045	0.945HisSer: 0.945 ± 0.048	0.844HisThr: 0.844 ± 0.04	1.008HisVal: 1.008 ± 0.046	0.182HisTrp: 0.182 ± 0.017	0.619HisTyr: 0.619 ± 0.032	0.0HisXaa: 0.0 ± 0.0
Ile	6.837IleAla: 6.837 ± 0.115	1.56IleCys: 1.56 ± 0.054	4.103IleAsp: 4.103 ± 0.083	4.377IleGlu: 4.377 ± 0.092	2.485IlePhe: 2.485 ± 0.079	5.275IleGly: 5.275 ± 0.116	0.979IleHis: 0.979 ± 0.043	4.927IleIle: 4.927 ± 0.127	3.749IleLys: 3.749 ± 0.09	5.522IleLeu: 5.522 ± 0.126	2.058IleMet: 2.058 ± 0.065	2.734IleAsn: 2.734 ± 0.068	2.889IlePro: 2.889 ± 0.071	1.298IleGln: 1.298 ± 0.047	2.873IleArg: 2.873 ± 0.07	4.949IleSer: 4.949 ± 0.095	3.542IleThr: 3.542 ± 0.087	5.183IleVal: 5.183 ± 0.111	0.575IleTrp: 0.575 ± 0.036	2.128IleTyr: 2.128 ± 0.063	0.0IleXaa: 0.0 ± 0.0
Lys	5.488LysAla: 5.488 ± 0.092	0.943LysCys: 0.943 ± 0.042	2.889LysAsp: 2.889 ± 0.085	3.549LysGlu: 3.549 ± 0.091	1.858LysPhe: 1.858 ± 0.064	3.481LysGly: 3.481 ± 0.089	0.997LysHis: 0.997 ± 0.042	3.699LysIle: 3.699 ± 0.076	4.303LysLys: 4.303 ± 0.095	5.903LysLeu: 5.903 ± 0.117	2.031LysMet: 2.031 ± 0.063	2.819LysAsn: 2.819 ± 0.069	2.256LysPro: 2.256 ± 0.061	1.82LysGln: 1.82 ± 0.062	3.026LysArg: 3.026 ± 0.083	3.196LysSer: 3.196 ± 0.081	3.596LysThr: 3.596 ± 0.091	3.546LysVal: 3.546 ± 0.094	0.534LysTrp: 0.534 ± 0.035	2.357LysTyr: 2.357 ± 0.065	0.0LysXaa: 0.0 ± 0.0
Leu	8.307LeuAla: 8.307 ± 0.159	2.142LeuCys: 2.142 ± 0.067	5.632LeuAsp: 5.632 ± 0.114	5.268LeuGlu: 5.268 ± 0.112	3.733LeuPhe: 3.733 ± 0.099	7.086LeuGly: 7.086 ± 0.135	1.592LeuHis: 1.592 ± 0.053	6.068LeuIle: 6.068 ± 0.137	4.958LeuLys: 4.958 ± 0.1	8.467LeuLeu: 8.467 ± 0.174	2.593LeuMet: 2.593 ± 0.075	3.264LeuAsn: 3.264 ± 0.085	3.892LeuPro: 3.892 ± 0.091	1.865LeuGln: 1.865 ± 0.062	4.981LeuArg: 4.981 ± 0.103	6.736LeuSer: 6.736 ± 0.122	5.077LeuThr: 5.077 ± 0.098	5.71LeuVal: 5.71 ± 0.106	0.842LeuTrp: 0.842 ± 0.045	2.875LeuTyr: 2.875 ± 0.088	0.002LeuXaa: 0.002 ± 0.002
Met	2.61MetAla: 2.61 ± 0.066	0.491MetCys: 0.491 ± 0.034	1.654MetAsp: 1.654 ± 0.054	1.715MetGlu: 1.715 ± 0.052	1.073MetPhe: 1.073 ± 0.046	2.092MetGly: 2.092 ± 0.067	0.61MetHis: 0.61 ± 0.036	1.876MetIle: 1.876 ± 0.061	2.105MetLys: 2.105 ± 0.06	3.311MetLeu: 3.311 ± 0.086	0.893MetMet: 0.893 ± 0.04	1.371MetAsn: 1.371 ± 0.054	1.428MetPro: 1.428 ± 0.047	1.017MetGln: 1.017 ± 0.044	1.717MetArg: 1.717 ± 0.055	2.218MetSer: 2.218 ± 0.072	1.748MetThr: 1.748 ± 0.056	1.683MetVal: 1.683 ± 0.056	0.242MetTrp: 0.242 ± 0.024	0.97MetTyr: 0.97 ± 0.043	0.0MetXaa: 0.0 ± 0.0
Asn	3.614AsnAla: 3.614 ± 0.09	0.716AsnCys: 0.716 ± 0.042	2.157AsnAsp: 2.157 ± 0.071	2.339AsnGlu: 2.339 ± 0.066	1.399AsnPhe: 1.399 ± 0.045	3.327AsnGly: 3.327 ± 0.08	0.579AsnHis: 0.579 ± 0.03	2.711AsnIle: 2.711 ± 0.062	1.913AsnLys: 1.913 ± 0.063	2.815AsnLeu: 2.815 ± 0.066	1.152AsnMet: 1.152 ± 0.044	1.376AsnAsn: 1.376 ± 0.057	1.573AsnPro: 1.573 ± 0.055	0.747AsnGln: 0.747 ± 0.037	1.711AsnArg: 1.711 ± 0.054	2.023AsnSer: 2.023 ± 0.062	1.919AsnThr: 1.919 ± 0.064	2.867AsnVal: 2.867 ± 0.067	0.353AsnTrp: 0.353 ± 0.028	1.425AsnTyr: 1.425 ± 0.051	0.004AsnXaa: 0.004 ± 0.003
Pro	3.179ProAla: 3.179 ± 0.085	0.647ProCys: 0.647 ± 0.039	2.662ProAsp: 2.662 ± 0.071	3.803ProGlu: 3.803 ± 0.097	1.499ProPhe: 1.499 ± 0.056	2.629ProGly: 2.629 ± 0.071	0.579ProHis: 0.579 ± 0.034	2.063ProIle: 2.063 ± 0.068	1.915ProLys: 1.915 ± 0.061	2.757ProLeu: 2.757 ± 0.065	1.035ProMet: 1.035 ± 0.041	1.228ProAsn: 1.228 ± 0.049	1.039ProPro: 1.039 ± 0.051	1.125ProGln: 1.125 ± 0.045	1.396ProArg: 1.396 ± 0.055	1.978ProSer: 1.978 ± 0.06	1.949ProThr: 1.949 ± 0.074	3.078ProVal: 3.078 ± 0.082	0.352ProTrp: 0.352 ± 0.025	1.333ProTyr: 1.333 ± 0.046	0.004ProXaa: 0.004 ± 0.003
Gln	2.251GlnAla: 2.251 ± 0.074	0.442GlnCys: 0.442 ± 0.03	1.093GlnAsp: 1.093 ± 0.041	1.416GlnGlu: 1.416 ± 0.055	0.99GlnPhe: 0.99 ± 0.038	1.553GlnGly: 1.553 ± 0.055	0.467GlnHis: 0.467 ± 0.03	1.861GlnIle: 1.861 ± 0.067	1.589GlnLys: 1.589 ± 0.058	2.763GlnLeu: 2.763 ± 0.078	0.938GlnMet: 0.938 ± 0.046	1.087GlnAsn: 1.087 ± 0.045	0.952GlnPro: 0.952 ± 0.046	0.904GlnGln: 0.904 ± 0.047	1.367GlnArg: 1.367 ± 0.05	1.648GlnSer: 1.648 ± 0.062	1.517GlnThr: 1.517 ± 0.051	1.486GlnVal: 1.486 ± 0.055	0.303GlnTrp: 0.303 ± 0.021	1.019GlnTyr: 1.019 ± 0.039	0.0GlnXaa: 0.0 ± 0.0
Arg	4.303ArgAla: 4.303 ± 0.098	0.936ArgCys: 0.936 ± 0.042	2.786ArgAsp: 2.786 ± 0.08	3.582ArgGlu: 3.582 ± 0.084	2.014ArgPhe: 2.014 ± 0.062	3.313ArgGly: 3.313 ± 0.093	0.929ArgHis: 0.929 ± 0.041	3.437ArgIle: 3.437 ± 0.085	2.851ArgLys: 2.851 ± 0.076	4.75ArgLeu: 4.75 ± 0.125	1.538ArgMet: 1.538 ± 0.053	1.605ArgAsn: 1.605 ± 0.049	1.506ArgPro: 1.506 ± 0.057	1.594ArgGln: 1.594 ± 0.05	3.381ArgArg: 3.381 ± 0.091	2.936ArgSer: 2.936 ± 0.084	2.325ArgThr: 2.325 ± 0.071	2.967ArgVal: 2.967 ± 0.07	0.456ArgTrp: 0.456 ± 0.03	1.899ArgTyr: 1.899 ± 0.058	0.002ArgXaa: 0.002 ± 0.002
Ser	6.105SerAla: 6.105 ± 0.095	1.115SerCys: 1.115 ± 0.046	3.811SerAsp: 3.811 ± 0.1	4.014SerGlu: 4.014 ± 0.088	2.894SerPhe: 2.894 ± 0.089	5.848SerGly: 5.848 ± 0.123	0.947SerHis: 0.947 ± 0.043	3.946SerIle: 3.946 ± 0.093	2.842SerLys: 2.842 ± 0.069	5.122SerLeu: 5.122 ± 0.101	1.863SerMet: 1.863 ± 0.063	1.686SerAsn: 1.686 ± 0.06	2.139SerPro: 2.139 ± 0.061	1.477SerGln: 1.477 ± 0.051	3.217SerArg: 3.217 ± 0.086	3.785SerSer: 3.785 ± 0.108	2.97SerThr: 2.97 ± 0.086	5.062SerVal: 5.062 ± 0.098	0.583SerTrp: 0.583 ± 0.039	2.287SerTyr: 2.287 ± 0.077	0.002SerXaa: 0.002 ± 0.002
Thr	5.275ThrAla: 5.275 ± 0.092	0.763ThrCys: 0.763 ± 0.046	3.156ThrAsp: 3.156 ± 0.073	3.345ThrGlu: 3.345 ± 0.08	1.942ThrPhe: 1.942 ± 0.072	4.64ThrGly: 4.64 ± 0.104	0.913ThrHis: 0.913 ± 0.043	3.142ThrIle: 3.142 ± 0.079	2.494ThrLys: 2.494 ± 0.065	4.871ThrLeu: 4.871 ± 0.103	1.416ThrMet: 1.416 ± 0.055	1.748ThrAsn: 1.748 ± 0.056	2.537ThrPro: 2.537 ± 0.076	1.327ThrGln: 1.327 ± 0.05	2.078ThrArg: 2.078 ± 0.068	2.747ThrSer: 2.747 ± 0.079	2.545ThrThr: 2.545 ± 0.076	5.046ThrVal: 5.046 ± 0.102	0.476ThrTrp: 0.476 ± 0.032	1.713ThrTyr: 1.713 ± 0.057	0.0ThrXaa: 0.0 ± 0.0
Val	5.847ValAla: 5.847 ± 0.115	1.823ValCys: 1.823 ± 0.065	3.735ValAsp: 3.735 ± 0.083	4.198ValGlu: 4.198 ± 0.086	3.214ValPhe: 3.214 ± 0.085	5.096ValGly: 5.096 ± 0.109	1.143ValHis: 1.143 ± 0.045	5.551ValIle: 5.551 ± 0.116	4.15ValLys: 4.15 ± 0.083	7.023ValLeu: 7.023 ± 0.125	2.13ValMet: 2.13 ± 0.065	2.57ValAsn: 2.57 ± 0.076	2.788ValPro: 2.788 ± 0.081	1.657ValGln: 1.657 ± 0.061	3.443ValArg: 3.443 ± 0.08	5.327ValSer: 5.327 ± 0.101	4.238ValThr: 4.238 ± 0.109	4.95ValVal: 4.95 ± 0.096	0.702ValTrp: 0.702 ± 0.033	2.507ValTyr: 2.507 ± 0.072	0.004ValXaa: 0.004 ± 0.002
Trp	0.77TrpAla: 0.77 ± 0.04	0.22TrpCys: 0.22 ± 0.02	0.543TrpAsp: 0.543 ± 0.028	0.545TrpGlu: 0.545 ± 0.03	0.435TrpPhe: 0.435 ± 0.031	0.691TrpGly: 0.691 ± 0.034	0.191TrpHis: 0.191 ± 0.021	0.552TrpIle: 0.552 ± 0.033	0.463TrpLys: 0.463 ± 0.031	1.039TrpLeu: 1.039 ± 0.05	0.298TrpMet: 0.298 ± 0.025	0.362TrpAsn: 0.362 ± 0.025	0.265TrpPro: 0.265 ± 0.022	0.328TrpGln: 0.328 ± 0.028	0.491TrpArg: 0.491 ± 0.031	0.494TrpSer: 0.494 ± 0.03	0.456TrpThr: 0.456 ± 0.028	0.507TrpVal: 0.507 ± 0.033	0.106TrpTrp: 0.106 ± 0.015	0.368TrpTyr: 0.368 ± 0.026	0.002TrpXaa: 0.002 ± 0.002
Tyr	3.165TyrAla: 3.165 ± 0.068	0.73TyrCys: 0.73 ± 0.034	2.527TyrAsp: 2.527 ± 0.075	2.245TyrGlu: 2.245 ± 0.068	1.628TyrPhe: 1.628 ± 0.056	2.837TyrGly: 2.837 ± 0.075	0.592TyrHis: 0.592 ± 0.032	2.487TyrIle: 2.487 ± 0.064	1.872TyrLys: 1.872 ± 0.057	3.05TyrLeu: 3.05 ± 0.078	1.089TyrMet: 1.089 ± 0.046	1.574TyrAsn: 1.574 ± 0.056	1.279TyrPro: 1.279 ± 0.052	0.806TyrGln: 0.806 ± 0.041	1.776TyrArg: 1.776 ± 0.057	2.294TyrSer: 2.294 ± 0.061	2.011TyrThr: 2.011 ± 0.069	2.453TyrVal: 2.453 ± 0.07	0.373TyrTrp: 0.373 ± 0.024	1.502TyrTyr: 1.502 ± 0.061	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.005XaaAla: 0.005 ± 0.003	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.005XaaGly: 0.005 ± 0.003	0.0XaaHis: 0.0 ± 0.0	0.004XaaIle: 0.004 ± 0.002	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.004XaaPro: 0.004 ± 0.002	0.0XaaGln: 0.0 ± 0.0	0.007XaaArg: 0.007 ± 0.003	0.002XaaSer: 0.002 ± 0.002	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.002XaaTyr: 0.002 ± 0.002	0.04XaaXaa: 0.04 ± 0.011

Statistics based on 1917 proteins (554504 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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