Richelia intracellularis HH01

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Nostocales; Nostocaceae; Richelia; Richelia intracellularis

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2278 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M1X2Q0|M1X2Q0_9NOST Uncharacterized protein OS=Richelia intracellularis HH01 OX=1165094 GN=RINTHH_10550 PE=4 SV=1
MM1 pKa = 7.02MVLVDD6 pKa = 4.37SYY8 pKa = 11.06DD9 pKa = 3.65TAVLVSGDD17 pKa = 3.51GNLEE21 pKa = 3.97YY22 pKa = 10.82VVNLDD27 pKa = 3.54GYY29 pKa = 9.69RR30 pKa = 11.84DD31 pKa = 3.8VIVVVVSFCSMTNDD45 pKa = 3.16RR46 pKa = 11.84LSNISNSYY54 pKa = 10.26IDD56 pKa = 3.96LEE58 pKa = 4.3EE59 pKa = 4.14VKK61 pKa = 10.78EE62 pKa = 4.55DD63 pKa = 3.51IQKK66 pKa = 9.53TSYY69 pKa = 10.98

Molecular weight: 7.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M1WZ77|M1WZ77_9NOST Expressed protein OS=Richelia intracellularis HH01 OX=1165094 GN=RINTHH_11920 PE=4 SV=1
MM1 pKa = 7.59RR2 pKa = 11.84PVKK5 pKa = 10.4RR6 pKa = 11.84SRR8 pKa = 11.84VNKK11 pKa = 9.63ARR13 pKa = 11.84SAGKK17 pKa = 9.47FRR19 pKa = 11.84KK20 pKa = 9.21QVGKK24 pKa = 8.83TKK26 pKa = 9.71MANLRR31 pKa = 11.84SNPMRR36 pKa = 11.84GGWRR40 pKa = 11.84LL41 pKa = 3.13

Molecular weight: 4.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2278

0

2278

598795

37

1823

262.9

29.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.956 ± 0.043

1.177 ± 0.017

4.544 ± 0.036

5.973 ± 0.045

3.909 ± 0.039

6.672 ± 0.043

2.063 ± 0.024

8.137 ± 0.045

5.562 ± 0.047

10.912 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.168 ± 0.021

4.87 ± 0.032

4.316 ± 0.03

4.81 ± 0.046

4.783 ± 0.041

6.635 ± 0.039

5.451 ± 0.029

6.433 ± 0.037

1.338 ± 0.023

3.29 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski