Mucor lusitanicus CBS 277.49
Average proteome isoelectric point is 6.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11586 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A162ZG98|A0A162ZG98_MUCCL NAD_binding_1 domain-containing protein OS=Mucor lusitanicus CBS 277.49 OX=747725 GN=MUCCIDRAFT_155309 PE=4 SV=1
MM1 pKa = 7.4 KK2 pKa = 10.63 LSTTKK7 pKa = 10.66 LVLFMVFVICLVATQVTAKK26 pKa = 9.82 HH27 pKa = 5.38 HH28 pKa = 6.96 HH29 pKa = 6.87 KK30 pKa = 11.03 DD31 pKa = 3.84 CDD33 pKa = 4.35 DD34 pKa = 5.28 DD35 pKa = 5.55 DD36 pKa = 6.67 HH37 pKa = 7.3 EE38 pKa = 5.98 DD39 pKa = 4.67 WDD41 pKa = 5.5 DD42 pKa = 3.7 DD43 pKa = 4.07 WKK45 pKa = 11.27 DD46 pKa = 4.07 DD47 pKa = 6.08 HH48 pKa = 7.99 DD49 pKa = 6.54 DD50 pKa = 4.64 DD51 pKa = 5.6 WNDD54 pKa = 4.73 DD55 pKa = 4.04 NDD57 pKa = 3.97 WDD59 pKa = 4.71 NNDD62 pKa = 3.83 WDD64 pKa = 4.69 HH65 pKa = 7.43 DD66 pKa = 4.09 NHH68 pKa = 7.1 PSSTWSSGWPVPTFGQSTAYY88 pKa = 9.75 YY89 pKa = 9.24 PPGAPATVTATYY101 pKa = 8.17 TQVYY105 pKa = 6.92 TATGVPTAFPNQPQYY120 pKa = 11.67 GSLASVTSVGVPVTLAAIMFTMAIVAA146 pKa = 4.21
Molecular weight: 16.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.744
IPC2_protein 3.935
IPC_protein 3.973
Toseland 3.719
ProMoST 4.177
Dawson 3.999
Bjellqvist 4.151
Wikipedia 4.012
Rodwell 3.795
Grimsley 3.63
Solomon 3.999
Lehninger 3.948
Nozaki 4.113
DTASelect 4.482
Thurlkill 3.808
EMBOSS 4.012
Sillero 4.113
Patrickios 1.1
IPC_peptide 3.986
IPC2_peptide 4.075
IPC2.peptide.svr19 3.942
Protein with the highest isoelectric point:
>tr|A0A168LW99|A0A168LW99_MUCCL Ubiquitin carboxyl-terminal hydrolase OS=Mucor lusitanicus CBS 277.49 OX=747725 GN=MUCCIDRAFT_91953 PE=3 SV=1
MM1 pKa = 7.57 VIIVTVIITMVITSITSMHH20 pKa = 6.45 IFIMIIVSIIAVVMVIIAAIARR42 pKa = 11.84 VISIIAVAISIIAAITTIAGVISIIAIVIVVVAVVISIIAVVISIIARR90 pKa = 11.84 VISNIAVVISIIAAITAIIPAVARR114 pKa = 11.84 VIIITSITAIVFVASSTRR132 pKa = 11.84 IRR134 pKa = 11.84 RR135 pKa = 11.84 ALAITTVAII144 pKa = 4.22
Molecular weight: 14.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.43
IPC2_protein 10.921
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.173
Rodwell 12.193
Grimsley 12.735
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 11.974
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.149
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11584
2
11586
4425880
49
4707
382.0
42.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.354 ± 0.019
1.461 ± 0.01
5.853 ± 0.017
5.965 ± 0.021
4.104 ± 0.017
5.243 ± 0.02
2.667 ± 0.011
5.878 ± 0.018
6.113 ± 0.023
9.191 ± 0.024
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.54 ± 0.009
4.645 ± 0.013
4.725 ± 0.018
4.678 ± 0.018
4.843 ± 0.015
7.882 ± 0.024
6.051 ± 0.016
6.174 ± 0.015
1.19 ± 0.009
3.441 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here