Actinokineospora terrae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Actinokineospora

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6615 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H9XPG1|A0A1H9XPG1_9PSEU Uncharacterized protein OS=Actinokineospora terrae OX=155974 GN=SAMN04487818_11871 PE=4 SV=1
MM1 pKa = 8.02PIGTTRR7 pKa = 11.84PRR9 pKa = 11.84AAIGRR14 pKa = 11.84RR15 pKa = 11.84VRR17 pKa = 11.84TLTCAVIMAAALPVVVGPPAQATFPGINGKK47 pKa = 9.65IYY49 pKa = 10.24FDD51 pKa = 3.83QDD53 pKa = 3.61DD54 pKa = 4.19EE55 pKa = 5.14VYY57 pKa = 10.73SINPDD62 pKa = 3.2GTGLTQLTDD71 pKa = 3.5SDD73 pKa = 5.12DD74 pKa = 3.66LSKK77 pKa = 11.09DD78 pKa = 3.2PRR80 pKa = 11.84VNADD84 pKa = 2.97GSKK87 pKa = 10.25VAFLRR92 pKa = 11.84YY93 pKa = 9.65SFSQGSNQVWVMNPDD108 pKa = 3.13GTGATQLTSAISADD122 pKa = 3.8SGSLAWSPAGDD133 pKa = 3.44KK134 pKa = 10.62LVYY137 pKa = 10.71SPDD140 pKa = 3.53LLTVIDD146 pKa = 5.3ADD148 pKa = 3.87GTDD151 pKa = 3.82PFSLGVSGGAPTWSPDD167 pKa = 3.03GTRR170 pKa = 11.84IAYY173 pKa = 6.56TASGGIRR180 pKa = 11.84AINPDD185 pKa = 3.0GTGQTTIKK193 pKa = 10.29NRR195 pKa = 11.84PSPPEE200 pKa = 3.96FLSGPSWSPDD210 pKa = 2.78GTKK213 pKa = 9.82IAASTYY219 pKa = 10.8DD220 pKa = 3.37FSTGQGGVFTINADD234 pKa = 3.21GTGYY238 pKa = 9.63TNISGTNTDD247 pKa = 3.93DD248 pKa = 4.51GAAVWSPDD256 pKa = 2.72GTKK259 pKa = 9.98ISFHH263 pKa = 7.41DD264 pKa = 4.03GTGLAVMNTNGTGRR278 pKa = 11.84TALHH282 pKa = 6.63SGFLNNGDD290 pKa = 3.52WAAVPAGADD299 pKa = 3.29VSVSVTDD306 pKa = 3.47SADD309 pKa = 3.54PVSLGGSPYY318 pKa = 9.73TYY320 pKa = 9.65TVSVANAGPAGATGVSVSTTLSGANRR346 pKa = 11.84TINSATSSQGSCTVSAPTVSCALGAVAASGSATVTISVTPSATGTITATSTVSATEE402 pKa = 3.87TDD404 pKa = 3.74PLSGNNTAAQSTTVNNALGCAITGTPGNDD433 pKa = 3.15TLNGGNGNDD442 pKa = 4.02VICGLGGNDD451 pKa = 4.65VINGGNNNDD460 pKa = 4.12TIYY463 pKa = 11.04AGAGDD468 pKa = 3.97DD469 pKa = 4.53TIDD472 pKa = 4.84GGNSDD477 pKa = 4.34DD478 pKa = 4.78TIHH481 pKa = 7.1AGDD484 pKa = 4.28GDD486 pKa = 4.28DD487 pKa = 5.12VIGGGNSADD496 pKa = 3.74YY497 pKa = 10.93LYY499 pKa = 11.45GEE501 pKa = 5.24AGNDD505 pKa = 3.34TNYY508 pKa = 11.11GEE510 pKa = 4.44TFLGSLLYY518 pKa = 11.11LFDD521 pKa = 4.45NGNDD525 pKa = 3.92HH526 pKa = 7.54IYY528 pKa = 10.76GGPGNDD534 pKa = 5.36DD535 pKa = 4.48LDD537 pKa = 4.22GQNGNDD543 pKa = 4.09TIVDD547 pKa = 3.62HH548 pKa = 7.68DD549 pKa = 4.21GTDD552 pKa = 3.02IMSGSNGNDD561 pKa = 3.46TIDD564 pKa = 3.46VLDD567 pKa = 4.24GVGGDD572 pKa = 3.74TANGGLGSDD581 pKa = 3.74TCAVDD586 pKa = 4.42GGDD589 pKa = 3.59TATGCC594 pKa = 4.36

Molecular weight: 59.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H9XRS5|A0A1H9XRS5_9PSEU Uncharacterized protein OS=Actinokineospora terrae OX=155974 GN=SAMN04487818_12310 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIIAARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84GRR40 pKa = 11.84DD41 pKa = 3.0KK42 pKa = 11.37LSAA45 pKa = 3.85

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6615

0

6615

2246422

39

7858

339.6

36.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.6 ± 0.044

0.754 ± 0.009

6.248 ± 0.024

5.094 ± 0.029

2.721 ± 0.018

9.102 ± 0.029

2.251 ± 0.015

3.249 ± 0.018

1.882 ± 0.024

10.461 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.516 ± 0.014

1.84 ± 0.018

6.007 ± 0.031

2.696 ± 0.019

7.814 ± 0.04

5.13 ± 0.026

6.689 ± 0.043

9.525 ± 0.034

1.52 ± 0.014

1.902 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski