Heliorestis convoluta

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Heliobacteriaceae; Heliorestis

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3259 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2MW37|A0A5Q2MW37_9FIRM Copper amine oxidase N-terminal domain-containing protein OS=Heliorestis convoluta OX=356322 GN=FTV88_0288 PE=4 SV=1
MM1 pKa = 7.87PSLKK5 pKa = 10.29EE6 pKa = 3.75RR7 pKa = 11.84ISYY10 pKa = 9.36IKK12 pKa = 10.77GMAEE16 pKa = 3.83GMKK19 pKa = 9.92IEE21 pKa = 4.43ADD23 pKa = 3.42PRR25 pKa = 11.84EE26 pKa = 4.05GRR28 pKa = 11.84LLSEE32 pKa = 5.2MITILDD38 pKa = 3.57TMAEE42 pKa = 4.16EE43 pKa = 4.14IEE45 pKa = 4.27EE46 pKa = 4.06VRR48 pKa = 11.84RR49 pKa = 11.84NQASLEE55 pKa = 4.01EE56 pKa = 4.24MVEE59 pKa = 4.48SIDD62 pKa = 3.8EE63 pKa = 4.26DD64 pKa = 4.65LVDD67 pKa = 5.54LEE69 pKa = 4.27NDD71 pKa = 3.72FYY73 pKa = 11.71DD74 pKa = 5.29LEE76 pKa = 5.18EE77 pKa = 6.36DD78 pKa = 5.03DD79 pKa = 6.72FDD81 pKa = 7.01DD82 pKa = 5.84EE83 pKa = 6.51DD84 pKa = 6.34FDD86 pKa = 7.55DD87 pKa = 6.47DD88 pKa = 5.46DD89 pKa = 7.21DD90 pKa = 7.32DD91 pKa = 7.33GILCDD96 pKa = 5.91CDD98 pKa = 6.09DD99 pKa = 5.8DD100 pKa = 7.08DD101 pKa = 7.43DD102 pKa = 5.51DD103 pKa = 5.8VIEE106 pKa = 5.21FEE108 pKa = 5.64CPTCNEE114 pKa = 4.27TVCFEE119 pKa = 4.64EE120 pKa = 5.23EE121 pKa = 4.51DD122 pKa = 4.1LDD124 pKa = 4.46DD125 pKa = 5.99DD126 pKa = 4.09EE127 pKa = 5.03VLEE130 pKa = 4.77VVCPTCDD137 pKa = 2.94HH138 pKa = 6.14TVYY141 pKa = 10.86VQDD144 pKa = 5.69GEE146 pKa = 4.43DD147 pKa = 4.17LDD149 pKa = 4.17MTACDD154 pKa = 5.76DD155 pKa = 4.49DD156 pKa = 7.13DD157 pKa = 4.97EE158 pKa = 6.21DD159 pKa = 5.07SPCFRR164 pKa = 11.84NNEE167 pKa = 3.88EE168 pKa = 4.03ATRR171 pKa = 11.84IPQSRR176 pKa = 11.84PPRR179 pKa = 11.84EE180 pKa = 3.95TYY182 pKa = 9.7RR183 pKa = 11.84ARR185 pKa = 11.84PYY187 pKa = 10.32HH188 pKa = 4.95YY189 pKa = 9.81HH190 pKa = 5.62HH191 pKa = 7.28HH192 pKa = 5.9NN193 pKa = 3.22

Molecular weight: 22.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2N406|A0A5Q2N406_9FIRM Nickel pincer cofactor biosynthesis protein LarC OS=Heliorestis convoluta OX=356322 GN=FTV88_0843 PE=4 SV=1
MM1 pKa = 7.32MAPLHH6 pKa = 6.65HH7 pKa = 6.71RR8 pKa = 11.84TQQRR12 pKa = 11.84IINQNPRR19 pKa = 11.84LHH21 pKa = 6.62ILNPLRR27 pKa = 11.84PMKK30 pKa = 10.42KK31 pKa = 8.94IASLHH36 pKa = 5.96SMHH39 pKa = 7.17LNPIIPLMRR48 pKa = 11.84QVRR51 pKa = 11.84ILKK54 pKa = 8.17TRR56 pKa = 11.84NHH58 pKa = 6.6QIHH61 pKa = 7.1PMPLIQKK68 pKa = 8.53PLHH71 pKa = 6.41NPLCMKK77 pKa = 10.07RR78 pKa = 11.84PRR80 pKa = 11.84RR81 pKa = 11.84ILGIIKK87 pKa = 8.56ITNNNNTHH95 pKa = 4.69QTTTSFSRR103 pKa = 11.84KK104 pKa = 6.82ITHH107 pKa = 6.93KK108 pKa = 10.29GTVLPPDD115 pKa = 4.04PPRR118 pKa = 11.84INHH121 pKa = 6.44NLLLQNLPIHH131 pKa = 6.45KK132 pKa = 9.41SKK134 pKa = 11.4SPMIYY139 pKa = 9.62QKK141 pKa = 10.35HH142 pKa = 5.99HH143 pKa = 6.84NISSLNRR150 pKa = 11.84LPHH153 pKa = 5.41IRR155 pKa = 11.84KK156 pKa = 7.28MHH158 pKa = 6.37TIITSQRR165 pKa = 11.84SRR167 pKa = 11.84QRR169 pKa = 11.84RR170 pKa = 11.84NMRR173 pKa = 11.84LHH175 pKa = 6.75HH176 pKa = 6.85IKK178 pKa = 10.16LAHH181 pKa = 6.54LVRR184 pKa = 11.84SQMTHH189 pKa = 5.27NLQSRR194 pKa = 11.84TLPQIINISLKK205 pKa = 10.5RR206 pKa = 11.84KK207 pKa = 7.15PQQRR211 pKa = 11.84NHH213 pKa = 6.41RR214 pKa = 11.84SAKK217 pKa = 7.67TLRR220 pKa = 11.84LLLNLTQHH228 pKa = 6.3MKK230 pKa = 10.32RR231 pKa = 11.84LPIIHH236 pKa = 6.65IPSRR240 pKa = 11.84PNQPRR245 pKa = 11.84LLRR248 pKa = 11.84RR249 pKa = 11.84RR250 pKa = 11.84IHH252 pKa = 5.8NKK254 pKa = 9.15PRR256 pKa = 11.84IHH258 pKa = 7.04CNTMTTHH265 pKa = 6.18PRR267 pKa = 11.84SRR269 pKa = 11.84LQNTHH274 pKa = 5.55PRR276 pKa = 11.84MAIGQINQFPHH287 pKa = 7.14IHH289 pKa = 6.02IQTLANQRR297 pKa = 11.84KK298 pKa = 9.56LIGKK302 pKa = 9.01SNIHH306 pKa = 4.4ITKK309 pKa = 10.47RR310 pKa = 11.84ILRR313 pKa = 11.84QLRR316 pKa = 11.84QLRR319 pKa = 11.84RR320 pKa = 11.84PSRR323 pKa = 11.84RR324 pKa = 11.84QQNLPLHH331 pKa = 6.12KK332 pKa = 10.32RR333 pKa = 11.84LIKK336 pKa = 9.54KK337 pKa = 7.61TSPLRR342 pKa = 11.84RR343 pKa = 11.84SQINAAHH350 pKa = 6.2NPVVINQLPQNLPRR364 pKa = 11.84QHH366 pKa = 7.5PLRR369 pKa = 11.84TMRR372 pKa = 11.84QIHH375 pKa = 6.7LPRR378 pKa = 11.84HH379 pKa = 4.49LQIRR383 pKa = 11.84PQLPQPLSNRR393 pKa = 11.84LCRR396 pKa = 11.84SHH398 pKa = 6.49RR399 pKa = 11.84TGRR402 pKa = 11.84LQNNQISRR410 pKa = 11.84LYY412 pKa = 9.97IGRR415 pKa = 11.84NSTRR419 pKa = 11.84RR420 pKa = 11.84RR421 pKa = 11.84LNIRR425 pKa = 11.84KK426 pKa = 8.65IRR428 pKa = 11.84HH429 pKa = 4.93MPLLKK434 pKa = 10.05RR435 pKa = 11.84RR436 pKa = 11.84RR437 pKa = 11.84HH438 pKa = 5.12RR439 pKa = 11.84QNKK442 pKa = 8.93NISLFRR448 pKa = 11.84IHH450 pKa = 7.65RR451 pKa = 11.84SLQIPTMDD459 pKa = 3.81SRR461 pKa = 11.84LHH463 pKa = 5.26QNIQIRR469 pKa = 11.84LINMDD474 pKa = 3.72LTTINRR480 pKa = 11.84LHH482 pKa = 6.84SLRR485 pKa = 11.84IHH487 pKa = 6.54INTMNTKK494 pKa = 7.51TLRR497 pKa = 11.84RR498 pKa = 3.8

Molecular weight: 59.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3259

0

3259

928659

31

2326

285.0

31.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.907 ± 0.053

0.965 ± 0.022

4.871 ± 0.029

7.734 ± 0.053

3.882 ± 0.029

6.823 ± 0.048

2.078 ± 0.019

7.045 ± 0.034

6.118 ± 0.049

10.322 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.603 ± 0.02

3.665 ± 0.039

4.029 ± 0.031

4.534 ± 0.049

5.342 ± 0.037

5.771 ± 0.032

5.211 ± 0.035

6.811 ± 0.039

1.074 ± 0.018

3.216 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski