Cronobacter phage vB_CsaP_009
Average proteome isoelectric point is 6.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 140 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A679FHZ9|A0A679FHZ9_9CAUD Uncharacterized protein OS=Cronobacter phage vB_CsaP_009 OX=2699738 PE=4 SV=1
MM1 pKa = 7.56 ACCDD5 pKa = 3.44 GWNLLSDD12 pKa = 4.05 GSKK15 pKa = 10.91 PNGVCPDD22 pKa = 4.2 CGVDD26 pKa = 3.53 TLDD29 pKa = 3.87 GDD31 pKa = 4.22 SVEE34 pKa = 4.14 GCFYY38 pKa = 11.26 SKK40 pKa = 10.4 VVCEE44 pKa = 4.38 TCGDD48 pKa = 3.91 APCDD52 pKa = 3.74 EE53 pKa = 4.76 SCC55 pKa = 5.14
Molecular weight: 5.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.999
IPC2_protein 3.478
IPC_protein 3.389
Toseland 3.198
ProMoST 3.63
Dawson 3.427
Bjellqvist 3.605
Wikipedia 3.439
Rodwell 3.249
Grimsley 3.121
Solomon 3.363
Lehninger 3.312
Nozaki 3.579
DTASelect 3.795
Thurlkill 3.3
EMBOSS 3.439
Sillero 3.528
Patrickios 0.006
IPC_peptide 3.35
IPC2_peptide 3.49
IPC2.peptide.svr19 3.647
Protein with the highest isoelectric point:
>tr|A0A679FGB6|A0A679FGB6_9CAUD Uncharacterized protein OS=Cronobacter phage vB_CsaP_009 OX=2699738 PE=4 SV=1
MM1 pKa = 7.46 ATLKK5 pKa = 10.82 YY6 pKa = 9.84 QVKK9 pKa = 9.87 KK10 pKa = 10.64 FKK12 pKa = 10.56 KK13 pKa = 9.4 SQIKK17 pKa = 8.91 SASMLINGDD26 pKa = 3.56 VYY28 pKa = 11.06 KK29 pKa = 11.13 GNNGDD34 pKa = 4.12 LYY36 pKa = 11.21 AVTGKK41 pKa = 8.46 TSRR44 pKa = 11.84 GLYY47 pKa = 9.38 VSNLRR52 pKa = 11.84 TGCSGYY58 pKa = 10.4 RR59 pKa = 11.84 SNKK62 pKa = 6.12 MHH64 pKa = 6.85 NPQAKK69 pKa = 9.14 PRR71 pKa = 11.84 LIVIGSIDD79 pKa = 3.47 ITSMQIRR86 pKa = 3.76
Molecular weight: 9.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.258
IPC2_protein 9.911
IPC_protein 10.116
Toseland 10.526
ProMoST 10.248
Dawson 10.657
Bjellqvist 10.292
Wikipedia 10.804
Rodwell 11.242
Grimsley 10.716
Solomon 10.687
Lehninger 10.657
Nozaki 10.496
DTASelect 10.292
Thurlkill 10.526
EMBOSS 10.906
Sillero 10.57
Patrickios 10.979
IPC_peptide 10.687
IPC2_peptide 9.004
IPC2.peptide.svr19 8.615
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
140
0
140
27768
37
1530
198.3
22.44
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.499 ± 0.277
1.167 ± 0.135
6.781 ± 0.2
7.336 ± 0.272
4.177 ± 0.194
6.594 ± 0.27
1.804 ± 0.095
6.706 ± 0.192
7.977 ± 0.284
7.808 ± 0.211
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.003 ± 0.103
5.946 ± 0.148
3.378 ± 0.149
3.126 ± 0.169
4.415 ± 0.156
7.375 ± 0.169
5.485 ± 0.167
6.457 ± 0.187
1.152 ± 0.107
3.814 ± 0.174
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here