Tobacco leaf curl Japan virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 7.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9JH37|Q9JH37_9GEMI Transcriptional activator protein OS=Tobacco leaf curl Japan virus OX=223332 GN=C2 PE=3 SV=1
MM1 pKa = 7.71APPKK5 pKa = 10.21RR6 pKa = 11.84FQINAKK12 pKa = 10.09NYY14 pKa = 8.13FLTYY18 pKa = 7.85PHH20 pKa = 7.15CSLTKK25 pKa = 10.57EE26 pKa = 4.09EE27 pKa = 4.94ALSQIQALDD36 pKa = 3.77TITNKK41 pKa = 10.3LFIRR45 pKa = 11.84ICRR48 pKa = 11.84EE49 pKa = 3.54LHH51 pKa = 6.34EE52 pKa = 5.86DD53 pKa = 4.22GSPHH57 pKa = 6.11LHH59 pKa = 6.16VLIQFEE65 pKa = 4.66GKK67 pKa = 9.91YY68 pKa = 10.04KK69 pKa = 9.85CTNQRR74 pKa = 11.84FFDD77 pKa = 4.07LVSPTRR83 pKa = 11.84SAHH86 pKa = 4.86FHH88 pKa = 6.55PNIQGAKK95 pKa = 9.45SSSDD99 pKa = 2.96VKK101 pKa = 11.2AYY103 pKa = 8.93MEE105 pKa = 4.44KK106 pKa = 10.83DD107 pKa = 3.21GDD109 pKa = 4.05VVDD112 pKa = 4.15YY113 pKa = 11.3GVFQVDD119 pKa = 3.12GRR121 pKa = 11.84SARR124 pKa = 11.84GGCQSANDD132 pKa = 4.26AYY134 pKa = 10.9AEE136 pKa = 4.72AINSGSKK143 pKa = 10.01SAALSILRR151 pKa = 11.84EE152 pKa = 3.96KK153 pKa = 10.75APKK156 pKa = 10.5DD157 pKa = 3.42FVLQFHH163 pKa = 6.68NLNSNLDD170 pKa = 4.22RR171 pKa = 11.84IFSPPLEE178 pKa = 4.1EE179 pKa = 4.45FVCPFLSSSFDD190 pKa = 3.35RR191 pKa = 11.84VPEE194 pKa = 3.92EE195 pKa = 4.02LEE197 pKa = 3.46EE198 pKa = 4.08WAAEE202 pKa = 4.06NARR205 pKa = 11.84DD206 pKa = 3.59SAARR210 pKa = 11.84PWRR213 pKa = 11.84PISIVIEE220 pKa = 4.23GDD222 pKa = 3.2SRR224 pKa = 11.84TGKK227 pKa = 8.52TMWARR232 pKa = 11.84SLSPHH237 pKa = 6.45HH238 pKa = 6.91NYY240 pKa = 10.59LCGHH244 pKa = 7.4LDD246 pKa = 4.18LSPKK250 pKa = 10.09VYY252 pKa = 10.85SNSAWYY258 pKa = 10.31NVIDD262 pKa = 5.38DD263 pKa = 4.41VDD265 pKa = 3.61PHH267 pKa = 5.85YY268 pKa = 11.0LKK270 pKa = 10.7HH271 pKa = 6.2FKK273 pKa = 10.7EE274 pKa = 4.46FMGAQRR280 pKa = 11.84DD281 pKa = 3.81WQSNTKK287 pKa = 9.26YY288 pKa = 10.63GKK290 pKa = 9.49PIQIKK295 pKa = 10.42GGIPTIFLCNPGPTSSYY312 pKa = 10.78TEE314 pKa = 4.02YY315 pKa = 11.19LDD317 pKa = 3.6EE318 pKa = 5.81DD319 pKa = 3.79KK320 pKa = 11.41NAALKK325 pKa = 10.41AWAIKK330 pKa = 9.87NASFVTLYY338 pKa = 10.95GPLYY342 pKa = 8.7STSHH346 pKa = 5.72QGSAQIRR353 pKa = 11.84EE354 pKa = 4.37EE355 pKa = 4.09EE356 pKa = 4.41STSQTEE362 pKa = 4.13DD363 pKa = 2.78

Molecular weight: 40.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9JH40|Q9JH40_9GEMI Protein V2 OS=Tobacco leaf curl Japan virus OX=223332 GN=V1 PE=3 SV=1
MM1 pKa = 7.71SKK3 pKa = 10.31RR4 pKa = 11.84PADD7 pKa = 3.24IGIFTPVSKK16 pKa = 10.56VRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNFDD24 pKa = 3.03SPYY27 pKa = 10.85ANRR30 pKa = 11.84ASAPIALATNRR41 pKa = 11.84RR42 pKa = 11.84RR43 pKa = 11.84SWAQRR48 pKa = 11.84PIYY51 pKa = 9.61RR52 pKa = 11.84KK53 pKa = 8.12PRR55 pKa = 11.84MYY57 pKa = 10.94RR58 pKa = 11.84MYY60 pKa = 10.57RR61 pKa = 11.84SPDD64 pKa = 3.29VPKK67 pKa = 10.81GCEE70 pKa = 4.39GPCKK74 pKa = 9.23VQSYY78 pKa = 7.95EE79 pKa = 3.85KK80 pKa = 10.56KK81 pKa = 10.39NDD83 pKa = 3.44VGHH86 pKa = 6.71SGTLLCVSDD95 pKa = 3.33ITRR98 pKa = 11.84GNGLTHH104 pKa = 7.19RR105 pKa = 11.84LGKK108 pKa = 10.02RR109 pKa = 11.84FCIKK113 pKa = 9.97SVYY116 pKa = 10.03IIGKK120 pKa = 8.43IWMDD124 pKa = 3.58EE125 pKa = 4.03NIKK128 pKa = 9.75MKK130 pKa = 10.73NHH132 pKa = 5.87TNNVLFWLVRR142 pKa = 11.84DD143 pKa = 4.17RR144 pKa = 11.84RR145 pKa = 11.84PGTTPYY151 pKa = 10.91GFQEE155 pKa = 4.25AFNMFEE161 pKa = 4.62NEE163 pKa = 3.81PSTATVKK170 pKa = 10.75QEE172 pKa = 3.83LRR174 pKa = 11.84DD175 pKa = 3.75RR176 pKa = 11.84LQVLHH181 pKa = 7.25RR182 pKa = 11.84FRR184 pKa = 11.84ATVTGGQYY192 pKa = 10.83ASKK195 pKa = 9.34EE196 pKa = 3.58QAIIKK201 pKa = 9.21RR202 pKa = 11.84FWKK205 pKa = 10.26LNHH208 pKa = 6.28HH209 pKa = 5.5VTYY212 pKa = 10.93NHH214 pKa = 6.12QEE216 pKa = 3.62QAKK219 pKa = 9.66YY220 pKa = 10.54EE221 pKa = 4.12NHH223 pKa = 6.33TEE225 pKa = 3.99NALLLYY231 pKa = 7.29MACTHH236 pKa = 7.07ASNPVYY242 pKa = 9.77ATLKK246 pKa = 9.27IRR248 pKa = 11.84VYY250 pKa = 10.64FYY252 pKa = 11.37DD253 pKa = 3.61SVV255 pKa = 3.37

Molecular weight: 29.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1100

97

363

183.3

20.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.182 ± 0.746

2.273 ± 0.442

5.091 ± 0.557

4.727 ± 0.852

4.273 ± 0.389

4.545 ± 0.609

3.818 ± 0.488

5.364 ± 0.575

5.455 ± 0.548

8.091 ± 0.71

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.0 ± 0.397

5.091 ± 0.604

5.636 ± 0.519

5.091 ± 0.645

6.818 ± 0.977

8.818 ± 1.27

6.091 ± 0.767

5.182 ± 0.702

1.455 ± 0.179

4.0 ± 0.633
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski