Butyricicoccus sp. 1XD8-22

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Butyricicoccus; unclassified Butyricicoccus

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10148 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A658JCX7|A0A658JCX7_9CLOT Long-chain fatty acid--CoA ligase (Fragment) OS=Butyricicoccus sp. 1XD8-22 OX=2320083 GN=D7X33_46345 PE=4 SV=1
AA1 pKa = 7.69YY2 pKa = 9.63DD3 pKa = 3.65GLTVVVNPEE12 pKa = 4.03NTWATEE18 pKa = 3.96LTVDD22 pKa = 3.85QLKK25 pKa = 10.55QIWIEE30 pKa = 4.37DD31 pKa = 3.67GTTKK35 pKa = 10.04KK36 pKa = 9.95WSDD39 pKa = 3.86IDD41 pKa = 3.92PSWPAEE47 pKa = 4.06EE48 pKa = 4.04IAFYY52 pKa = 11.15APGTDD57 pKa = 2.85SGTYY61 pKa = 9.98DD62 pKa = 3.49YY63 pKa = 10.79FDD65 pKa = 3.58EE66 pKa = 5.19VILEE70 pKa = 4.02GAEE73 pKa = 3.81IVKK76 pKa = 10.22NAEE79 pKa = 3.94LSEE82 pKa = 4.53DD83 pKa = 4.13DD84 pKa = 3.62NTLVMGVSGNKK95 pKa = 9.1NAIGFFGYY103 pKa = 10.47AYY105 pKa = 10.75YY106 pKa = 11.0LEE108 pKa = 4.8NQDD111 pKa = 3.82KK112 pKa = 8.92LTAVTVDD119 pKa = 3.88GVAPNNQTIEE129 pKa = 4.21SGEE132 pKa = 4.05YY133 pKa = 9.34TPLSRR138 pKa = 11.84PLFVYY143 pKa = 10.66ASNSALKK150 pKa = 10.28EE151 pKa = 3.49NAAFYY156 pKa = 11.13DD157 pKa = 4.0FMEE160 pKa = 4.75FTLEE164 pKa = 4.1NAGDD168 pKa = 3.66MAEE171 pKa = 4.04EE172 pKa = 3.99VGYY175 pKa = 10.99VRR177 pKa = 11.84LPDD180 pKa = 4.01EE181 pKa = 4.96IYY183 pKa = 11.15AEE185 pKa = 4.4GLATLEE191 pKa = 4.1GLKK194 pKa = 10.62

Molecular weight: 21.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A658JS82|A0A658JS82_9CLOT Anaerobic ribonucleoside-triphosphate reductase OS=Butyricicoccus sp. 1XD8-22 OX=2320083 GN=nrdD PE=4 SV=1
MM1 pKa = 7.73RR2 pKa = 11.84SISGSGAKK10 pKa = 10.12APAGAGQSPAAARR23 pKa = 11.84AGLGSRR29 pKa = 11.84GNAPALPAQGWGPGATPRR47 pKa = 11.84RR48 pKa = 11.84RR49 pKa = 11.84PRR51 pKa = 11.84RR52 pKa = 11.84AGVQGQRR59 pKa = 11.84PGAARR64 pKa = 11.84AGLGSRR70 pKa = 11.84GKK72 pKa = 10.71APALPAA78 pKa = 4.52

Molecular weight: 7.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10148

0

10148

2192787

16

3207

216.1

24.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.484 ± 0.044

1.261 ± 0.012

5.352 ± 0.025

7.311 ± 0.031

4.208 ± 0.02

7.252 ± 0.032

1.884 ± 0.013

6.791 ± 0.035

5.797 ± 0.031

9.599 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.649 ± 0.014

4.058 ± 0.023

3.912 ± 0.02

3.633 ± 0.017

5.086 ± 0.029

5.871 ± 0.02

5.483 ± 0.029

6.777 ± 0.026

1.001 ± 0.008

3.594 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski