Asbolus verrucosus

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Coleoptera; Polyphaga; Cucujiformia; Tenebrionoidea; Tenebrionidae;

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 15225 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482W0Z4|A0A482W0Z4_9CUCU Protein hunchback OS=Asbolus verrucosus OX=1661398 GN=BDFB_004443 PE=3 SV=1
MM1 pKa = 7.4LFEE4 pKa = 4.4VVFVFHH10 pKa = 6.48YY11 pKa = 9.83LQRR14 pKa = 11.84TCPLCSKK21 pKa = 10.31HH22 pKa = 6.97LSLKK26 pKa = 10.24QINFTTAVYY35 pKa = 9.94ICEE38 pKa = 4.81DD39 pKa = 3.86LQCNYY44 pKa = 10.01PIGYY48 pKa = 9.52DD49 pKa = 3.63CITVEE54 pKa = 5.34RR55 pKa = 11.84KK56 pKa = 9.8FEE58 pKa = 5.06DD59 pKa = 3.19MDD61 pKa = 5.19KK62 pKa = 11.0DD63 pKa = 3.94IEE65 pKa = 4.23LLNNRR70 pKa = 11.84EE71 pKa = 3.89EE72 pKa = 5.78DD73 pKa = 4.06LIDD76 pKa = 4.18WIDD79 pKa = 4.14EE80 pKa = 4.1IVEE83 pKa = 4.17KK84 pKa = 11.33SNDD87 pKa = 3.53ANSSEE92 pKa = 4.56TNTSLTEE99 pKa = 4.02LPVTDD104 pKa = 4.17EE105 pKa = 4.45TTSEE109 pKa = 5.37DD110 pKa = 3.4PTNWIDD116 pKa = 5.32SIINSNADD124 pKa = 3.55DD125 pKa = 4.66DD126 pKa = 5.6LSTSVVSNVGEE137 pKa = 4.27VTQVNSDD144 pKa = 3.68TNEE147 pKa = 3.83DD148 pKa = 3.57NAISSISFDD157 pKa = 4.63DD158 pKa = 3.85IVDD161 pKa = 4.14FVYY164 pKa = 11.04NIDD167 pKa = 3.34

Molecular weight: 19.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482VFH7|A0A482VFH7_9CUCU Uncharacterized protein OS=Asbolus verrucosus OX=1661398 GN=BDFB_010571 PE=4 SV=1
MM1 pKa = 6.91VRR3 pKa = 11.84APGTTFHH10 pKa = 6.49LQTRR14 pKa = 11.84KK15 pKa = 9.9RR16 pKa = 11.84FRR18 pKa = 11.84ILPRR22 pKa = 11.84SRR24 pKa = 11.84FRR26 pKa = 11.84GGRR29 pKa = 3.32

Molecular weight: 3.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

15225

0

15225

5816570

8

10722

382.0

43.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.712 ± 0.017

2.025 ± 0.019

5.387 ± 0.015

6.772 ± 0.03

4.286 ± 0.019

5.255 ± 0.026

2.439 ± 0.011

6.295 ± 0.017

7.115 ± 0.028

9.336 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.214 ± 0.009

5.433 ± 0.019

4.8 ± 0.023

4.061 ± 0.016

4.838 ± 0.018

7.465 ± 0.022

5.683 ± 0.018

6.331 ± 0.017

1.11 ± 0.007

3.425 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski