Mycobacterium phage Mdavu

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Timquatrovirus; unclassified Timquatrovirus

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 78 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A649VLU8|A0A649VLU8_9CAUD Uncharacterized protein OS=Mycobacterium phage Mdavu OX=2656595 GN=33 PE=4 SV=1
MM1 pKa = 8.09DD2 pKa = 5.43APTHH6 pKa = 5.1FTAVASTGDD15 pKa = 3.51EE16 pKa = 4.33PGWLLVHH23 pKa = 6.26VPEE26 pKa = 5.77IDD28 pKa = 3.29QYY30 pKa = 9.19TQARR34 pKa = 11.84TLDD37 pKa = 3.64EE38 pKa = 4.8VGPMAADD45 pKa = 6.26LIATWLNVPVEE56 pKa = 4.4SVDD59 pKa = 4.08VEE61 pKa = 4.63VKK63 pKa = 9.71YY64 pKa = 9.62TDD66 pKa = 3.01

Molecular weight:
7.23 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A649VN02|A0A649VN02_9CAUD Uncharacterized protein OS=Mycobacterium phage Mdavu OX=2656595 GN=48 PE=4 SV=1
MM1 pKa = 7.0PCYY4 pKa = 10.12RR5 pKa = 11.84RR6 pKa = 11.84THH8 pKa = 5.41GFEE11 pKa = 4.33LRR13 pKa = 11.84ICVGTGARR21 pKa = 11.84GGPRR25 pKa = 11.84RR26 pKa = 11.84RR27 pKa = 11.84SQQRR31 pKa = 11.84VQSRR35 pKa = 11.84SEE37 pKa = 4.15CVADD41 pKa = 3.0IHH43 pKa = 6.48TPLVVLGIWTFFHH56 pKa = 5.62VVSRR60 pKa = 11.84AVNRR64 pKa = 11.84CFRR67 pKa = 11.84RR68 pKa = 11.84SVPIYY73 pKa = 10.0DD74 pKa = 3.87QISIPGSSTLKK85 pKa = 10.9SPGQRR90 pKa = 11.84HH91 pKa = 5.46FLWPGLFYY99 pKa = 10.75IHH101 pKa = 6.57TPSHH105 pKa = 6.19KK106 pKa = 9.78SLRR109 pKa = 11.84SATMASLRR117 pKa = 11.84TGSRR121 pKa = 11.84KK122 pKa = 10.39DD123 pKa = 3.1GTTYY127 pKa = 8.99TQVRR131 pKa = 11.84YY132 pKa = 9.85RR133 pKa = 11.84LNGKK137 pKa = 7.22QTSTSFDD144 pKa = 3.6DD145 pKa = 3.74PAQAVEE151 pKa = 4.86FKK153 pKa = 11.16RR154 pKa = 11.84MVEE157 pKa = 3.72QLGAAKK163 pKa = 9.9ALEE166 pKa = 4.61VIEE169 pKa = 4.39STEE172 pKa = 3.75ATAQHH177 pKa = 5.66YY178 pKa = 7.57TVGDD182 pKa = 3.64WLDD185 pKa = 3.79HH186 pKa = 5.61YY187 pKa = 10.59LSHH190 pKa = 6.37KK191 pKa = 9.54TGVEE195 pKa = 3.53KK196 pKa = 10.38STMYY200 pKa = 10.58DD201 pKa = 3.23YY202 pKa = 11.63RR203 pKa = 11.84KK204 pKa = 9.14MVEE207 pKa = 3.85KK208 pKa = 10.62DD209 pKa = 2.84IAPVLGPIPLAALTAEE225 pKa = 4.6DD226 pKa = 3.03VAMWVKK232 pKa = 10.63GLAARR237 pKa = 11.84GLKK240 pKa = 10.35GKK242 pKa = 8.65TIANKK247 pKa = 10.32HH248 pKa = 5.45GFLSSALKK256 pKa = 10.03VAASRR261 pKa = 11.84GLIKK265 pKa = 10.8SNPAVGGVGMVEE277 pKa = 4.11IPRR280 pKa = 11.84SEE282 pKa = 4.0RR283 pKa = 11.84EE284 pKa = 3.63EE285 pKa = 4.01MVFLTRR291 pKa = 11.84EE292 pKa = 3.65QYY294 pKa = 11.16ARR296 pKa = 11.84LRR298 pKa = 11.84DD299 pKa = 4.07CVTEE303 pKa = 3.8PWRR306 pKa = 11.84PLVEE310 pKa = 4.14FLVASGARR318 pKa = 11.84WGEE321 pKa = 4.04VTALRR326 pKa = 11.84PSDD329 pKa = 3.63VNRR332 pKa = 11.84DD333 pKa = 3.36EE334 pKa = 4.11GTVRR338 pKa = 11.84ISRR341 pKa = 11.84AWKK344 pKa = 8.46RR345 pKa = 11.84TYY347 pKa = 8.71EE348 pKa = 4.0TGGYY352 pKa = 9.78EE353 pKa = 3.6IGAPKK358 pKa = 9.2TDD360 pKa = 3.04KK361 pKa = 10.73SRR363 pKa = 11.84RR364 pKa = 11.84TINVDD369 pKa = 3.2PSVLDD374 pKa = 3.43QLDD377 pKa = 3.89YY378 pKa = 11.61SGDD381 pKa = 3.19WLFTNRR387 pKa = 11.84SGGPVRR393 pKa = 11.84HH394 pKa = 6.39NGFHH398 pKa = 6.54DD399 pKa = 4.16RR400 pKa = 11.84VWRR403 pKa = 11.84PAIKK407 pKa = 10.31RR408 pKa = 11.84ADD410 pKa = 3.71LGVKK414 pKa = 9.82PRR416 pKa = 11.84VHH418 pKa = 7.38DD419 pKa = 4.41LRR421 pKa = 11.84HH422 pKa = 5.77TCASWLIAAGLPLPAIQQHH441 pKa = 6.61LGHH444 pKa = 6.69EE445 pKa = 4.8SIQVTIGVYY454 pKa = 9.36GHH456 pKa = 7.43LDD458 pKa = 3.46RR459 pKa = 11.84SHH461 pKa = 5.77GRR463 pKa = 11.84AVAAAIAAQLDD474 pKa = 4.1RR475 pKa = 11.84NN476 pKa = 3.99

Molecular weight:
53.05 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

78

0

78

17446

39

1377

223.7

24.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.696 ± 0.566

1.089 ± 0.145

6.454 ± 0.255

6.087 ± 0.334

2.648 ± 0.187

8.661 ± 0.532

2.218 ± 0.216

3.772 ± 0.162

3.193 ± 0.226

8.294 ± 0.223

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.086 ± 0.117

2.786 ± 0.236

5.583 ± 0.291

3.256 ± 0.194

7.549 ± 0.377

5.228 ± 0.215

5.589 ± 0.195

8.34 ± 0.383

2.155 ± 0.105

2.316 ± 0.198

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski