Mycobacterium phage Mdavu
Average proteome isoelectric point is 6.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 78 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A649VLU8|A0A649VLU8_9CAUD Uncharacterized protein OS=Mycobacterium phage Mdavu OX=2656595 GN=33 PE=4 SV=1
MM1 pKa = 8.09 DD2 pKa = 5.43 APTHH6 pKa = 5.1 FTAVASTGDD15 pKa = 3.51 EE16 pKa = 4.33 PGWLLVHH23 pKa = 6.26 VPEE26 pKa = 5.77 IDD28 pKa = 3.29 QYY30 pKa = 9.19 TQARR34 pKa = 11.84 TLDD37 pKa = 3.64 EE38 pKa = 4.8 VGPMAADD45 pKa = 6.26 LIATWLNVPVEE56 pKa = 4.4 SVDD59 pKa = 4.08 VEE61 pKa = 4.63 VKK63 pKa = 9.71 YY64 pKa = 9.62 TDD66 pKa = 3.01
Molecular weight: 7.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.072
IPC2_protein 3.884
IPC_protein 3.783
Toseland 3.592
ProMoST 3.859
Dawson 3.783
Bjellqvist 4.024
Wikipedia 3.732
Rodwell 3.617
Grimsley 3.503
Solomon 3.757
Lehninger 3.706
Nozaki 3.923
DTASelect 4.113
Thurlkill 3.668
EMBOSS 3.745
Sillero 3.91
Patrickios 3.121
IPC_peptide 3.757
IPC2_peptide 3.884
IPC2.peptide.svr19 3.87
Protein with the highest isoelectric point:
>tr|A0A649VN02|A0A649VN02_9CAUD Uncharacterized protein OS=Mycobacterium phage Mdavu OX=2656595 GN=48 PE=4 SV=1
MM1 pKa = 7.0 PCYY4 pKa = 10.12 RR5 pKa = 11.84 RR6 pKa = 11.84 THH8 pKa = 5.41 GFEE11 pKa = 4.33 LRR13 pKa = 11.84 ICVGTGARR21 pKa = 11.84 GGPRR25 pKa = 11.84 RR26 pKa = 11.84 RR27 pKa = 11.84 SQQRR31 pKa = 11.84 VQSRR35 pKa = 11.84 SEE37 pKa = 4.15 CVADD41 pKa = 3.0 IHH43 pKa = 6.48 TPLVVLGIWTFFHH56 pKa = 5.62 VVSRR60 pKa = 11.84 AVNRR64 pKa = 11.84 CFRR67 pKa = 11.84 RR68 pKa = 11.84 SVPIYY73 pKa = 10.0 DD74 pKa = 3.87 QISIPGSSTLKK85 pKa = 10.9 SPGQRR90 pKa = 11.84 HH91 pKa = 5.46 FLWPGLFYY99 pKa = 10.75 IHH101 pKa = 6.57 TPSHH105 pKa = 6.19 KK106 pKa = 9.78 SLRR109 pKa = 11.84 SATMASLRR117 pKa = 11.84 TGSRR121 pKa = 11.84 KK122 pKa = 10.39 DD123 pKa = 3.1 GTTYY127 pKa = 8.99 TQVRR131 pKa = 11.84 YY132 pKa = 9.85 RR133 pKa = 11.84 LNGKK137 pKa = 7.22 QTSTSFDD144 pKa = 3.6 DD145 pKa = 3.74 PAQAVEE151 pKa = 4.86 FKK153 pKa = 11.16 RR154 pKa = 11.84 MVEE157 pKa = 3.72 QLGAAKK163 pKa = 9.9 ALEE166 pKa = 4.61 VIEE169 pKa = 4.39 STEE172 pKa = 3.75 ATAQHH177 pKa = 5.66 YY178 pKa = 7.57 TVGDD182 pKa = 3.64 WLDD185 pKa = 3.79 HH186 pKa = 5.61 YY187 pKa = 10.59 LSHH190 pKa = 6.37 KK191 pKa = 9.54 TGVEE195 pKa = 3.53 KK196 pKa = 10.38 STMYY200 pKa = 10.58 DD201 pKa = 3.23 YY202 pKa = 11.63 RR203 pKa = 11.84 KK204 pKa = 9.14 MVEE207 pKa = 3.85 KK208 pKa = 10.62 DD209 pKa = 2.84 IAPVLGPIPLAALTAEE225 pKa = 4.6 DD226 pKa = 3.03 VAMWVKK232 pKa = 10.63 GLAARR237 pKa = 11.84 GLKK240 pKa = 10.35 GKK242 pKa = 8.65 TIANKK247 pKa = 10.32 HH248 pKa = 5.45 GFLSSALKK256 pKa = 10.03 VAASRR261 pKa = 11.84 GLIKK265 pKa = 10.8 SNPAVGGVGMVEE277 pKa = 4.11 IPRR280 pKa = 11.84 SEE282 pKa = 4.0 RR283 pKa = 11.84 EE284 pKa = 3.63 EE285 pKa = 4.01 MVFLTRR291 pKa = 11.84 EE292 pKa = 3.65 QYY294 pKa = 11.16 ARR296 pKa = 11.84 LRR298 pKa = 11.84 DD299 pKa = 4.07 CVTEE303 pKa = 3.8 PWRR306 pKa = 11.84 PLVEE310 pKa = 4.14 FLVASGARR318 pKa = 11.84 WGEE321 pKa = 4.04 VTALRR326 pKa = 11.84 PSDD329 pKa = 3.63 VNRR332 pKa = 11.84 DD333 pKa = 3.36 EE334 pKa = 4.11 GTVRR338 pKa = 11.84 ISRR341 pKa = 11.84 AWKK344 pKa = 8.46 RR345 pKa = 11.84 TYY347 pKa = 8.71 EE348 pKa = 4.0 TGGYY352 pKa = 9.78 EE353 pKa = 3.6 IGAPKK358 pKa = 9.2 TDD360 pKa = 3.04 KK361 pKa = 10.73 SRR363 pKa = 11.84 RR364 pKa = 11.84 TINVDD369 pKa = 3.2 PSVLDD374 pKa = 3.43 QLDD377 pKa = 3.89 YY378 pKa = 11.61 SGDD381 pKa = 3.19 WLFTNRR387 pKa = 11.84 SGGPVRR393 pKa = 11.84 HH394 pKa = 6.39 NGFHH398 pKa = 6.54 DD399 pKa = 4.16 RR400 pKa = 11.84 VWRR403 pKa = 11.84 PAIKK407 pKa = 10.31 RR408 pKa = 11.84 ADD410 pKa = 3.71 LGVKK414 pKa = 9.82 PRR416 pKa = 11.84 VHH418 pKa = 7.38 DD419 pKa = 4.41 LRR421 pKa = 11.84 HH422 pKa = 5.77 TCASWLIAAGLPLPAIQQHH441 pKa = 6.61 LGHH444 pKa = 6.69 EE445 pKa = 4.8 SIQVTIGVYY454 pKa = 9.36 GHH456 pKa = 7.43 LDD458 pKa = 3.46 RR459 pKa = 11.84 SHH461 pKa = 5.77 GRR463 pKa = 11.84 AVAAAIAAQLDD474 pKa = 4.1 RR475 pKa = 11.84 NN476 pKa = 3.99
Molecular weight: 53.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.244
IPC2_protein 9.297
IPC_protein 9.502
Toseland 10.014
ProMoST 9.75
Dawson 10.233
Bjellqvist 9.94
Wikipedia 10.409
Rodwell 10.482
Grimsley 10.306
Solomon 10.262
Lehninger 10.218
Nozaki 10.043
DTASelect 9.911
Thurlkill 10.072
EMBOSS 10.423
Sillero 10.145
Patrickios 9.853
IPC_peptide 10.262
IPC2_peptide 8.785
IPC2.peptide.svr19 8.261
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
78
0
78
17446
39
1377
223.7
24.31
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.696 ± 0.566
1.089 ± 0.145
6.454 ± 0.255
6.087 ± 0.334
2.648 ± 0.187
8.661 ± 0.532
2.218 ± 0.216
3.772 ± 0.162
3.193 ± 0.226
8.294 ± 0.223
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.086 ± 0.117
2.786 ± 0.236
5.583 ± 0.291
3.256 ± 0.194
7.549 ± 0.377
5.228 ± 0.215
5.589 ± 0.195
8.34 ± 0.383
2.155 ± 0.105
2.316 ± 0.198
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here