Polaribacter irgensii 23-P

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Polaribacter; Polaribacter irgensii

Average proteome isoelectric point is 7.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2556 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A4BW98|A4BW98_9FLAO Uroporphyrinogen III methylase OS=Polaribacter irgensii 23-P OX=313594 GN=PI23P_02057 PE=4 SV=1
MM1 pKa = 7.42FGYY4 pKa = 9.85SQTPITDD11 pKa = 3.72ANIQTAINTCLSTKK25 pKa = 10.31PEE27 pKa = 4.55DD28 pKa = 4.09GMCSEE33 pKa = 4.62SEE35 pKa = 4.08YY36 pKa = 11.05GIMPEE41 pKa = 3.69WDD43 pKa = 3.07VSQVTNISNAFLGKK57 pKa = 8.91TDD59 pKa = 4.09FNGDD63 pKa = 2.96INGWDD68 pKa = 3.56VSNVTNMTCMFLEE81 pKa = 4.18VGSFNKK87 pKa = 10.23DD88 pKa = 2.89VSDD91 pKa = 3.64WNEE94 pKa = 3.85SSVTNMNDD102 pKa = 2.91LFANTTAFNKK112 pKa = 10.22PMGDD116 pKa = 2.65WDD118 pKa = 3.79VRR120 pKa = 11.84NVTNMTSMFQGASSFNGGYY139 pKa = 10.45KK140 pKa = 10.42FLGCEE145 pKa = 3.79

Molecular weight: 16.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A4BWD6|A4BWD6_9FLAO Putative sigma factor A OS=Polaribacter irgensii 23-P OX=313594 GN=PI23P_02247 PE=4 SV=1
MM1 pKa = 7.5HH2 pKa = 7.39GLAASALQIRR12 pKa = 11.84RR13 pKa = 11.84SSMNGRR19 pKa = 11.84VLLAPNLVDD28 pKa = 5.84LSWIKK33 pKa = 10.39CLKK36 pKa = 7.93HH37 pKa = 5.54QNYY40 pKa = 9.66SRR42 pKa = 11.84VYY44 pKa = 10.4LLL46 pKa = 4.45

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2556

0

2556

790124

21

2395

309.1

34.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.941 ± 0.049

0.725 ± 0.015

5.0 ± 0.035

6.473 ± 0.052

5.516 ± 0.042

6.33 ± 0.043

1.753 ± 0.022

8.324 ± 0.043

8.365 ± 0.06

9.419 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.178 ± 0.027

5.95 ± 0.048

3.131 ± 0.022

3.328 ± 0.026

3.396 ± 0.03

6.436 ± 0.037

5.813 ± 0.037

6.212 ± 0.042

0.957 ± 0.017

3.753 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski