Acidiphilium sp. CAG:727

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Acetobacteraceae; Acidiphilium; environmental samples

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1479 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7G4K3|R7G4K3_9PROT Hydrolase_4 domain-containing protein OS=Acidiphilium sp. CAG:727 OX=1262689 GN=BN767_00229 PE=4 SV=1
MM1 pKa = 7.51NGNISIVIGSWGSYY15 pKa = 8.56NEE17 pKa = 4.16CNEE20 pKa = 4.02RR21 pKa = 11.84ALGSKK26 pKa = 9.0WLDD29 pKa = 3.21LSDD32 pKa = 3.73YY33 pKa = 10.86SDD35 pKa = 2.95WDD37 pKa = 4.17EE38 pKa = 4.63IVEE41 pKa = 4.08EE42 pKa = 4.19LKK44 pKa = 11.02KK45 pKa = 10.97EE46 pKa = 4.0GFEE49 pKa = 4.42LDD51 pKa = 4.56GIDD54 pKa = 4.36EE55 pKa = 4.35EE56 pKa = 6.22LFIQDD61 pKa = 4.33IEE63 pKa = 4.9GIDD66 pKa = 3.88DD67 pKa = 4.98GSINWDD73 pKa = 3.38YY74 pKa = 11.81VNPEE78 pKa = 3.98EE79 pKa = 5.88LFNTLNDD86 pKa = 3.78SGVLDD91 pKa = 4.88DD92 pKa = 4.43DD93 pKa = 3.93EE94 pKa = 6.38KK95 pKa = 11.89YY96 pKa = 10.83EE97 pKa = 4.14VFCAFLEE104 pKa = 4.63VRR106 pKa = 11.84CFDD109 pKa = 4.0DD110 pKa = 3.95FEE112 pKa = 4.61EE113 pKa = 4.45RR114 pKa = 11.84VKK116 pKa = 11.19SRR118 pKa = 11.84GHH120 pKa = 6.36RR121 pKa = 11.84WNDD124 pKa = 4.84DD125 pKa = 2.72IDD127 pKa = 3.28LWRR130 pKa = 11.84GYY132 pKa = 10.25DD133 pKa = 3.11WEE135 pKa = 5.74DD136 pKa = 3.26YY137 pKa = 9.81GKK139 pKa = 11.15EE140 pKa = 3.74MLEE143 pKa = 4.06CCGYY147 pKa = 10.46QIPDD151 pKa = 4.48HH152 pKa = 6.93ILDD155 pKa = 4.95FIDD158 pKa = 3.44LEE160 pKa = 4.51RR161 pKa = 11.84YY162 pKa = 9.08GRR164 pKa = 11.84YY165 pKa = 9.67CGEE168 pKa = 4.61EE169 pKa = 3.78YY170 pKa = 10.5LQEE173 pKa = 4.09YY174 pKa = 10.58SEE176 pKa = 4.49GLIEE180 pKa = 4.23IQQ182 pKa = 4.22

Molecular weight: 21.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7G356|R7G356_9PROT 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) OS=Acidiphilium sp. CAG:727 OX=1262689 GN=ispG PE=3 SV=1
MM1 pKa = 7.23IAAASRR7 pKa = 11.84GSQISEE13 pKa = 4.03IGRR16 pKa = 11.84HH17 pKa = 4.65SAKK20 pKa = 10.01PHH22 pKa = 5.28NARR25 pKa = 11.84LLIEE29 pKa = 3.98ISHH32 pKa = 7.54RR33 pKa = 11.84LRR35 pKa = 11.84QSHH38 pKa = 7.24IIFIRR43 pKa = 11.84QKK45 pKa = 10.8LDD47 pKa = 3.25DD48 pKa = 4.32SGIEE52 pKa = 3.99VARR55 pKa = 11.84SRR57 pKa = 11.84SHH59 pKa = 6.73RR60 pKa = 11.84NAAEE64 pKa = 3.71RR65 pKa = 11.84SEE67 pKa = 4.25THH69 pKa = 6.3GRR71 pKa = 11.84IHH73 pKa = 6.92TLTALNRR80 pKa = 11.84RR81 pKa = 11.84NRR83 pKa = 11.84RR84 pKa = 11.84AVTEE88 pKa = 3.96VHH90 pKa = 6.64SNNLKK95 pKa = 9.68IRR97 pKa = 11.84VVFSAEE103 pKa = 3.46ILSRR107 pKa = 11.84FCGNEE112 pKa = 3.58SVARR116 pKa = 11.84SVEE119 pKa = 4.38TVSSYY124 pKa = 11.34LIIGVRR130 pKa = 11.84FVRR133 pKa = 11.84DD134 pKa = 3.5TVDD137 pKa = 2.86VSLFGHH143 pKa = 5.58SVVEE147 pKa = 4.19SRR149 pKa = 11.84IEE151 pKa = 4.22NPYY154 pKa = 9.25HH155 pKa = 6.08WDD157 pKa = 3.12VRR159 pKa = 11.84HH160 pKa = 5.92YY161 pKa = 10.88SLTSPYY167 pKa = 8.91PDD169 pKa = 3.05NVRR172 pKa = 11.84GLMKK176 pKa = 10.28RR177 pKa = 11.84SEE179 pKa = 4.15FGKK182 pKa = 8.86TFDD185 pKa = 3.56SLHH188 pKa = 6.66NIFSS192 pKa = 3.77

Molecular weight: 21.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1479

0

1479

481004

29

2735

325.2

36.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.901 ± 0.065

1.564 ± 0.026

6.141 ± 0.049

6.901 ± 0.066

4.736 ± 0.049

7.281 ± 0.05

1.213 ± 0.025

6.999 ± 0.06

8.199 ± 0.052

8.24 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.128 ± 0.031

4.932 ± 0.048

2.923 ± 0.03

1.975 ± 0.034

4.063 ± 0.054

6.054 ± 0.048

5.789 ± 0.069

7.6 ± 0.059

0.737 ± 0.02

4.619 ± 0.055
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski