Amino acid dipepetide frequency for Acidiphilium sp. CAG:727

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.748AlaAla: 6.748 ± 0.154	1.21AlaCys: 1.21 ± 0.051	5.32AlaAsp: 5.32 ± 0.118	6.27AlaGlu: 6.27 ± 0.145	3.977AlaPhe: 3.977 ± 0.126	5.786AlaGly: 5.786 ± 0.131	0.965AlaHis: 0.965 ± 0.045	5.262AlaIle: 5.262 ± 0.121	6.062AlaLys: 6.062 ± 0.132	7.106AlaLeu: 7.106 ± 0.129	1.73AlaMet: 1.73 ± 0.068	3.212AlaAsn: 3.212 ± 0.081	1.807AlaPro: 1.807 ± 0.057	1.628AlaGln: 1.628 ± 0.055	2.956AlaArg: 2.956 ± 0.091	3.836AlaSer: 3.836 ± 0.103	4.482AlaThr: 4.482 ± 0.136	6.827AlaVal: 6.827 ± 0.125	0.397AlaTrp: 0.397 ± 0.033	3.416AlaTyr: 3.416 ± 0.101	0.006AlaXaa: 0.006 ± 0.004
Cys	1.22CysAla: 1.22 ± 0.055	0.218CysCys: 0.218 ± 0.024	1.089CysAsp: 1.089 ± 0.054	0.99CysGlu: 0.99 ± 0.052	0.767CysPhe: 0.767 ± 0.039	1.753CysGly: 1.753 ± 0.064	0.247CysHis: 0.247 ± 0.023	0.838CysIle: 0.838 ± 0.037	1.185CysLys: 1.185 ± 0.054	1.185CysLeu: 1.185 ± 0.05	0.277CysMet: 0.277 ± 0.023	0.609CysAsn: 0.609 ± 0.036	0.578CysPro: 0.578 ± 0.033	0.225CysGln: 0.225 ± 0.025	0.63CysArg: 0.63 ± 0.042	1.046CysSer: 1.046 ± 0.046	0.709CysThr: 0.709 ± 0.062	1.345CysVal: 1.345 ± 0.048	0.11CysTrp: 0.11 ± 0.014	0.615CysTyr: 0.615 ± 0.034	0.0CysXaa: 0.0 ± 0.0
Asp	4.096AspAla: 4.096 ± 0.107	1.039AspCys: 1.039 ± 0.046	3.875AspAsp: 3.875 ± 0.118	5.813AspGlu: 5.813 ± 0.124	3.301AspPhe: 3.301 ± 0.077	6.071AspGly: 6.071 ± 0.142	0.582AspHis: 0.582 ± 0.03	4.062AspIle: 4.062 ± 0.095	5.389AspLys: 5.389 ± 0.121	4.609AspLeu: 4.609 ± 0.107	1.401AspMet: 1.401 ± 0.057	2.827AspAsn: 2.827 ± 0.088	1.449AspPro: 1.449 ± 0.057	0.528AspGln: 0.528 ± 0.031	2.216AspArg: 2.216 ± 0.076	2.649AspSer: 2.649 ± 0.081	2.78AspThr: 2.78 ± 0.076	4.792AspVal: 4.792 ± 0.111	0.478AspTrp: 0.478 ± 0.035	3.447AspTyr: 3.447 ± 0.092	0.004AspXaa: 0.004 ± 0.003
Glu	4.26GluAla: 4.26 ± 0.119	0.854GluCys: 0.854 ± 0.038	3.601GluAsp: 3.601 ± 0.105	5.193GluGlu: 5.193 ± 0.153	3.006GluPhe: 3.006 ± 0.082	3.437GluGly: 3.437 ± 0.095	0.85GluHis: 0.85 ± 0.047	6.306GluIle: 6.306 ± 0.143	7.53GluLys: 7.53 ± 0.172	5.584GluLeu: 5.584 ± 0.12	1.59GluMet: 1.59 ± 0.063	5.1GluAsn: 5.1 ± 0.108	1.491GluPro: 1.491 ± 0.06	1.597GluGln: 1.597 ± 0.068	3.268GluArg: 3.268 ± 0.092	3.333GluSer: 3.333 ± 0.095	3.902GluThr: 3.902 ± 0.101	4.089GluVal: 4.089 ± 0.098	0.624GluTrp: 0.624 ± 0.037	3.391GluTyr: 3.391 ± 0.11	0.002GluXaa: 0.002 ± 0.002
Phe	4.274PheAla: 4.274 ± 0.107	0.909PheCys: 0.909 ± 0.05	3.247PheAsp: 3.247 ± 0.079	2.736PheGlu: 2.736 ± 0.076	2.241PhePhe: 2.241 ± 0.092	3.985PheGly: 3.985 ± 0.103	0.568PheHis: 0.568 ± 0.03	3.197PheIle: 3.197 ± 0.093	2.807PheLys: 2.807 ± 0.076	3.428PheLeu: 3.428 ± 0.103	0.942PheMet: 0.942 ± 0.047	2.195PheAsn: 2.195 ± 0.075	1.312PhePro: 1.312 ± 0.056	0.736PheGln: 0.736 ± 0.035	1.875PheArg: 1.875 ± 0.064	3.553PheSer: 3.553 ± 0.095	2.973PheThr: 2.973 ± 0.093	3.823PheVal: 3.823 ± 0.099	0.366PheTrp: 0.366 ± 0.03	2.193PheTyr: 2.193 ± 0.078	0.0PheXaa: 0.0 ± 0.0
Gly	5.351GlyAla: 5.351 ± 0.133	1.243GlyCys: 1.243 ± 0.06	4.393GlyAsp: 4.393 ± 0.108	5.719GlyGlu: 5.719 ± 0.118	3.403GlyPhe: 3.403 ± 0.085	5.628GlyGly: 5.628 ± 0.128	0.871GlyHis: 0.871 ± 0.047	4.9GlyIle: 4.9 ± 0.108	7.145GlyLys: 7.145 ± 0.129	5.451GlyLeu: 5.451 ± 0.117	1.63GlyMet: 1.63 ± 0.057	3.732GlyAsn: 3.732 ± 0.125	0.786GlyPro: 0.786 ± 0.038	1.47GlyGln: 1.47 ± 0.055	2.94GlyArg: 2.94 ± 0.094	3.919GlySer: 3.919 ± 0.105	3.663GlyThr: 3.663 ± 0.123	6.15GlyVal: 6.15 ± 0.118	0.669GlyTrp: 0.669 ± 0.043	3.744GlyTyr: 3.744 ± 0.093	0.0GlyXaa: 0.0 ± 0.0
His	0.958HisAla: 0.958 ± 0.046	0.26HisCys: 0.26 ± 0.024	0.686HisAsp: 0.686 ± 0.041	0.723HisGlu: 0.723 ± 0.039	0.605HisPhe: 0.605 ± 0.035	0.977HisGly: 0.977 ± 0.04	0.245HisHis: 0.245 ± 0.027	0.881HisIle: 0.881 ± 0.043	0.784HisLys: 0.784 ± 0.04	0.863HisLeu: 0.863 ± 0.037	0.191HisMet: 0.191 ± 0.021	0.636HisAsn: 0.636 ± 0.035	0.588HisPro: 0.588 ± 0.041	0.208HisGln: 0.208 ± 0.022	0.499HisArg: 0.499 ± 0.034	0.792HisSer: 0.792 ± 0.043	0.73HisThr: 0.73 ± 0.039	0.79HisVal: 0.79 ± 0.038	0.106HisTrp: 0.106 ± 0.016	0.599HisTyr: 0.599 ± 0.037	0.004HisXaa: 0.004 ± 0.003
Ile	6.052IleAla: 6.052 ± 0.13	1.162IleCys: 1.162 ± 0.059	4.547IleAsp: 4.547 ± 0.095	4.555IleGlu: 4.555 ± 0.11	3.062IlePhe: 3.062 ± 0.091	4.821IleGly: 4.821 ± 0.126	0.701IleHis: 0.701 ± 0.04	5.012IleIle: 5.012 ± 0.13	5.524IleLys: 5.524 ± 0.118	5.47IleLeu: 5.47 ± 0.12	1.349IleMet: 1.349 ± 0.055	3.385IleAsn: 3.385 ± 0.081	2.632IlePro: 2.632 ± 0.082	1.094IleGln: 1.094 ± 0.054	2.775IleArg: 2.775 ± 0.085	4.792IleSer: 4.792 ± 0.114	4.713IleThr: 4.713 ± 0.117	5.243IleVal: 5.243 ± 0.116	0.437IleTrp: 0.437 ± 0.033	2.667IleTyr: 2.667 ± 0.082	0.0IleXaa: 0.0 ± 0.0
Lys	5.728LysAla: 5.728 ± 0.13	1.0LysCys: 1.0 ± 0.051	4.642LysAsp: 4.642 ± 0.108	5.809LysGlu: 5.809 ± 0.143	3.345LysPhe: 3.345 ± 0.079	4.732LysGly: 4.732 ± 0.107	0.915LysHis: 0.915 ± 0.043	6.771LysIle: 6.771 ± 0.127	7.391LysLys: 7.391 ± 0.162	6.378LysLeu: 6.378 ± 0.13	1.886LysMet: 1.886 ± 0.062	5.272LysAsn: 5.272 ± 0.104	2.374LysPro: 2.374 ± 0.068	1.813LysGln: 1.813 ± 0.074	3.896LysArg: 3.896 ± 0.107	4.744LysSer: 4.744 ± 0.091	5.715LysThr: 5.715 ± 0.117	5.077LysVal: 5.077 ± 0.111	0.576LysTrp: 0.576 ± 0.032	3.925LysTyr: 3.925 ± 0.094	0.002LysXaa: 0.002 ± 0.002
Leu	6.466LeuAla: 6.466 ± 0.121	1.509LeuCys: 1.509 ± 0.056	4.59LeuAsp: 4.59 ± 0.118	4.162LeuGlu: 4.162 ± 0.114	3.996LeuPhe: 3.996 ± 0.105	5.509LeuGly: 5.509 ± 0.131	0.977LeuHis: 0.977 ± 0.046	5.407LeuIle: 5.407 ± 0.132	7.152LeuLys: 7.152 ± 0.146	6.842LeuLeu: 6.842 ± 0.151	1.825LeuMet: 1.825 ± 0.079	3.906LeuAsn: 3.906 ± 0.093	3.416LeuPro: 3.416 ± 0.092	1.744LeuGln: 1.744 ± 0.061	3.457LeuArg: 3.457 ± 0.105	6.753LeuSer: 6.753 ± 0.149	5.628LeuThr: 5.628 ± 0.139	5.031LeuVal: 5.031 ± 0.11	0.647LeuTrp: 0.647 ± 0.039	3.38LeuTyr: 3.38 ± 0.097	0.004LeuXaa: 0.004 ± 0.003
Met	1.511MetAla: 1.511 ± 0.058	0.241MetCys: 0.241 ± 0.024	0.996MetAsp: 0.996 ± 0.05	0.938MetGlu: 0.938 ± 0.05	0.859MetPhe: 0.859 ± 0.045	1.17MetGly: 1.17 ± 0.055	0.27MetHis: 0.27 ± 0.024	1.499MetIle: 1.499 ± 0.059	2.173MetLys: 2.173 ± 0.069	2.087MetLeu: 2.087 ± 0.071	0.52MetMet: 0.52 ± 0.038	1.239MetAsn: 1.239 ± 0.051	0.975MetPro: 0.975 ± 0.036	0.732MetGln: 0.732 ± 0.038	1.156MetArg: 1.156 ± 0.053	1.462MetSer: 1.462 ± 0.063	1.441MetThr: 1.441 ± 0.052	1.135MetVal: 1.135 ± 0.054	0.164MetTrp: 0.164 ± 0.018	0.709MetTyr: 0.709 ± 0.039	0.0MetXaa: 0.0 ± 0.0
Asn	4.455AsnAla: 4.455 ± 0.108	0.784AsnCys: 0.784 ± 0.053	2.985AsnAsp: 2.985 ± 0.077	3.457AsnGlu: 3.457 ± 0.083	2.276AsnPhe: 2.276 ± 0.076	4.96AsnGly: 4.96 ± 0.14	0.615AsnHis: 0.615 ± 0.038	2.954AsnIle: 2.954 ± 0.075	3.534AsnLys: 3.534 ± 0.089	4.131AsnLeu: 4.131 ± 0.107	1.054AsnMet: 1.054 ± 0.045	2.062AsnAsn: 2.062 ± 0.087	1.817AsnPro: 1.817 ± 0.065	0.83AsnGln: 0.83 ± 0.043	1.815AsnArg: 1.815 ± 0.058	2.713AsnSer: 2.713 ± 0.077	2.087AsnThr: 2.087 ± 0.071	4.01AsnVal: 4.01 ± 0.103	0.341AsnTrp: 0.341 ± 0.028	2.437AsnTyr: 2.437 ± 0.081	0.0AsnXaa: 0.0 ± 0.0
Pro	2.139ProAla: 2.139 ± 0.074	0.455ProCys: 0.455 ± 0.036	2.279ProAsp: 2.279 ± 0.078	2.491ProGlu: 2.491 ± 0.084	1.484ProPhe: 1.484 ± 0.056	1.385ProGly: 1.385 ± 0.052	0.464ProHis: 0.464 ± 0.032	1.906ProIle: 1.906 ± 0.068	1.975ProLys: 1.975 ± 0.064	2.516ProLeu: 2.516 ± 0.07	0.593ProMet: 0.593 ± 0.034	1.222ProAsn: 1.222 ± 0.048	0.713ProPro: 0.713 ± 0.039	0.761ProGln: 0.761 ± 0.044	0.877ProArg: 0.877 ± 0.041	1.906ProSer: 1.906 ± 0.067	1.877ProThr: 1.877 ± 0.067	2.634ProVal: 2.634 ± 0.081	0.216ProTrp: 0.216 ± 0.022	1.337ProTyr: 1.337 ± 0.057	0.0ProXaa: 0.0 ± 0.0
Gln	1.422GlnAla: 1.422 ± 0.064	0.214GlnCys: 0.214 ± 0.021	0.983GlnAsp: 0.983 ± 0.043	1.102GlnGlu: 1.102 ± 0.056	0.854GlnPhe: 0.854 ± 0.043	1.262GlnGly: 1.262 ± 0.055	0.239GlnHis: 0.239 ± 0.023	1.672GlnIle: 1.672 ± 0.058	1.78GlnLys: 1.78 ± 0.064	1.613GlnLeu: 1.613 ± 0.065	0.505GlnMet: 0.505 ± 0.036	1.197GlnAsn: 1.197 ± 0.048	0.582GlnPro: 0.582 ± 0.033	0.468GlnGln: 0.468 ± 0.035	1.006GlnArg: 1.006 ± 0.057	1.27GlnSer: 1.27 ± 0.051	1.362GlnThr: 1.362 ± 0.054	1.162GlnVal: 1.162 ± 0.05	0.177GlnTrp: 0.177 ± 0.022	0.875GlnTyr: 0.875 ± 0.042	0.0GlnXaa: 0.0 ± 0.0
Arg	2.913ArgAla: 2.913 ± 0.082	0.522ArgCys: 0.522 ± 0.036	2.091ArgAsp: 2.091 ± 0.075	2.83ArgGlu: 2.83 ± 0.096	1.948ArgPhe: 1.948 ± 0.069	2.484ArgGly: 2.484 ± 0.075	0.574ArgHis: 0.574 ± 0.039	3.245ArgIle: 3.245 ± 0.088	3.688ArgLys: 3.688 ± 0.096	3.732ArgLeu: 3.732 ± 0.109	0.967ArgMet: 0.967 ± 0.042	2.002ArgAsn: 2.002 ± 0.071	1.104ArgPro: 1.104 ± 0.045	1.162ArgGln: 1.162 ± 0.054	2.029ArgArg: 2.029 ± 0.081	2.222ArgSer: 2.222 ± 0.075	2.012ArgThr: 2.012 ± 0.062	2.655ArgVal: 2.655 ± 0.083	0.281ArgTrp: 0.281 ± 0.022	2.164ArgTyr: 2.164 ± 0.073	0.002ArgXaa: 0.002 ± 0.002
Ser	5.528SerAla: 5.528 ± 0.13	0.863SerCys: 0.863 ± 0.052	4.214SerAsp: 4.214 ± 0.097	4.162SerGlu: 4.162 ± 0.094	3.05SerPhe: 3.05 ± 0.092	5.331SerGly: 5.331 ± 0.112	0.8SerHis: 0.8 ± 0.036	3.441SerIle: 3.441 ± 0.118	3.694SerLys: 3.694 ± 0.094	5.628SerLeu: 5.628 ± 0.123	1.131SerMet: 1.131 ± 0.055	2.087SerAsn: 2.087 ± 0.078	1.711SerPro: 1.711 ± 0.059	1.254SerGln: 1.254 ± 0.052	2.299SerArg: 2.299 ± 0.07	3.815SerSer: 3.815 ± 0.095	2.711SerThr: 2.711 ± 0.07	5.767SerVal: 5.767 ± 0.135	0.478SerTrp: 0.478 ± 0.034	2.574SerTyr: 2.574 ± 0.077	0.002SerXaa: 0.002 ± 0.002
Thr	5.449ThrAla: 5.449 ± 0.17	0.844ThrCys: 0.844 ± 0.043	3.881ThrAsp: 3.881 ± 0.091	4.239ThrGlu: 4.239 ± 0.106	2.815ThrPhe: 2.815 ± 0.089	4.262ThrGly: 4.262 ± 0.09	0.763ThrHis: 0.763 ± 0.035	3.807ThrIle: 3.807 ± 0.108	3.179ThrLys: 3.179 ± 0.085	5.534ThrLeu: 5.534 ± 0.112	0.956ThrMet: 0.956 ± 0.05	2.089ThrAsn: 2.089 ± 0.069	2.112ThrPro: 2.112 ± 0.066	1.252ThrGln: 1.252 ± 0.059	1.884ThrArg: 1.884 ± 0.073	3.085ThrSer: 3.085 ± 0.104	3.021ThrThr: 3.021 ± 0.102	5.99ThrVal: 5.99 ± 0.165	0.331ThrTrp: 0.331 ± 0.029	2.399ThrTyr: 2.399 ± 0.103	0.002ThrXaa: 0.002 ± 0.002
Val	6.017ValAla: 6.017 ± 0.129	1.449ValCys: 1.449 ± 0.054	4.094ValAsp: 4.094 ± 0.094	4.493ValGlu: 4.493 ± 0.101	3.661ValPhe: 3.661 ± 0.102	5.091ValGly: 5.091 ± 0.123	0.811ValHis: 0.811 ± 0.043	5.532ValIle: 5.532 ± 0.126	6.888ValLys: 6.888 ± 0.145	6.058ValLeu: 6.058 ± 0.121	1.655ValMet: 1.655 ± 0.061	3.794ValAsn: 3.794 ± 0.093	2.208ValPro: 2.208 ± 0.07	1.164ValGln: 1.164 ± 0.051	2.988ValArg: 2.988 ± 0.088	5.098ValSer: 5.098 ± 0.112	5.089ValThr: 5.089 ± 0.143	5.711ValVal: 5.711 ± 0.127	0.57ValTrp: 0.57 ± 0.034	3.628ValTyr: 3.628 ± 0.081	0.004ValXaa: 0.004 ± 0.003
Trp	0.549TrpAla: 0.549 ± 0.037	0.11TrpCys: 0.11 ± 0.014	0.385TrpAsp: 0.385 ± 0.029	0.366TrpGlu: 0.366 ± 0.026	0.36TrpPhe: 0.36 ± 0.024	0.526TrpGly: 0.526 ± 0.033	0.166TrpHis: 0.166 ± 0.02	0.509TrpIle: 0.509 ± 0.031	0.524TrpLys: 0.524 ± 0.03	0.757TrpLeu: 0.757 ± 0.04	0.175TrpMet: 0.175 ± 0.019	0.503TrpAsn: 0.503 ± 0.038	0.106TrpPro: 0.106 ± 0.018	0.331TrpGln: 0.331 ± 0.025	0.258TrpArg: 0.258 ± 0.023	0.41TrpSer: 0.41 ± 0.033	0.422TrpThr: 0.422 ± 0.029	0.405TrpVal: 0.405 ± 0.034	0.112TrpTrp: 0.112 ± 0.017	0.397TrpTyr: 0.397 ± 0.029	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.867TyrAla: 3.867 ± 0.092	0.748TyrCys: 0.748 ± 0.043	3.503TyrAsp: 3.503 ± 0.093	2.96TyrGlu: 2.96 ± 0.07	2.349TyrPhe: 2.349 ± 0.075	3.636TyrGly: 3.636 ± 0.093	0.503TyrHis: 0.503 ± 0.033	2.79TyrIle: 2.79 ± 0.075	3.58TyrLys: 3.58 ± 0.091	3.428TyrLeu: 3.428 ± 0.089	0.894TyrMet: 0.894 ± 0.043	2.245TyrAsn: 2.245 ± 0.067	1.47TyrPro: 1.47 ± 0.067	0.753TyrGln: 0.753 ± 0.043	1.798TyrArg: 1.798 ± 0.065	3.141TyrSer: 3.141 ± 0.091	2.576TyrThr: 2.576 ± 0.106	3.364TyrVal: 3.364 ± 0.1	0.291TyrTrp: 0.291 ± 0.029	2.289TyrTyr: 2.289 ± 0.094	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.002XaaAla: 0.002 ± 0.002	0.0XaaCys: 0.0 ± 0.0	0.006XaaAsp: 0.006 ± 0.003	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.002XaaGly: 0.002 ± 0.002	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.008XaaLys: 0.008 ± 0.004	0.002XaaLeu: 0.002 ± 0.002	0.004XaaMet: 0.004 ± 0.003	0.002XaaAsn: 0.002 ± 0.002	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.004XaaArg: 0.004 ± 0.003	0.002XaaSer: 0.002 ± 0.002	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.033XaaXaa: 0.033 ± 0.01

Statistics based on 1479 proteins (481004 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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