Hubei sobemo-like virus 8

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEH3|A0A1L3KEH3_9VIRU RNA-directed RNA polymerase OS=Hubei sobemo-like virus 8 OX=1923241 PE=4 SV=1
MM1 pKa = 7.53TSTPGCCILAKK12 pKa = 10.49LGSTNAQIFKK22 pKa = 9.94WDD24 pKa = 4.14GVTMDD29 pKa = 4.97PDD31 pKa = 2.9RR32 pKa = 11.84LAYY35 pKa = 9.63VRR37 pKa = 11.84AVVRR41 pKa = 11.84QRR43 pKa = 11.84TLSLLEE49 pKa = 4.43SPVADD54 pKa = 3.95PLNVFVKK61 pKa = 10.1PEE63 pKa = 3.66PHH65 pKa = 6.14SAKK68 pKa = 10.43KK69 pKa = 9.72MEE71 pKa = 4.25EE72 pKa = 3.54GRR74 pKa = 11.84YY75 pKa = 9.32RR76 pKa = 11.84LISAVSLVDD85 pKa = 3.44TLVDD89 pKa = 3.17RR90 pKa = 11.84VLFGWLGRR98 pKa = 11.84VSLDD102 pKa = 3.36SVGRR106 pKa = 11.84TPCLVGWSPISGGWRR121 pKa = 11.84QLKK124 pKa = 9.8ALYY127 pKa = 9.93RR128 pKa = 11.84GEE130 pKa = 4.24VLCLDD135 pKa = 3.85KK136 pKa = 11.31SAWDD140 pKa = 3.48WSVQGWMVDD149 pKa = 2.84MWKK152 pKa = 8.52TFVKK156 pKa = 10.16EE157 pKa = 3.65LAFGHH162 pKa = 5.83SDD164 pKa = 2.49WWGKK168 pKa = 8.79MVDD171 pKa = 3.49QRR173 pKa = 11.84FKK175 pKa = 11.5LLFEE179 pKa = 4.54DD180 pKa = 6.05AIFQFKK186 pKa = 10.84DD187 pKa = 3.19GTQVFQGSPGIMKK200 pKa = 10.02SGCYY204 pKa = 8.46LTILLNSVGQSFVHH218 pKa = 5.66YY219 pKa = 9.9LANLSLGRR227 pKa = 11.84EE228 pKa = 4.08PTQNQPHH235 pKa = 7.05TIGDD239 pKa = 3.88DD240 pKa = 3.66TVQGADD246 pKa = 3.1IDD248 pKa = 4.15FEE250 pKa = 5.22VYY252 pKa = 10.29VEE254 pKa = 4.39AIKK257 pKa = 10.88RR258 pKa = 11.84LGFRR262 pKa = 11.84VKK264 pKa = 10.23GAHH267 pKa = 5.58IRR269 pKa = 11.84KK270 pKa = 8.77NPEE273 pKa = 3.2FAGFCFGKK281 pKa = 8.3TCWPAYY287 pKa = 5.79WQKK290 pKa = 11.09HH291 pKa = 4.8LFAMAYY297 pKa = 9.7AQDD300 pKa = 4.51LGEE303 pKa = 4.33MMHH306 pKa = 8.14SYY308 pKa = 9.55MPLYY312 pKa = 10.66VNEE315 pKa = 3.91PVMFNLLRR323 pKa = 11.84RR324 pKa = 11.84VAFEE328 pKa = 4.67LNPATVLTVGEE339 pKa = 4.69CRR341 pKa = 11.84EE342 pKa = 4.16IMNGAA347 pKa = 3.56

Molecular weight: 39.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEH3|A0A1L3KEH3_9VIRU RNA-directed RNA polymerase OS=Hubei sobemo-like virus 8 OX=1923241 PE=4 SV=1
MM1 pKa = 7.49SSTMSSLISLLKK13 pKa = 10.78SFLSDD18 pKa = 2.91TCRR21 pKa = 11.84FVMTLVIMFLIWPHH35 pKa = 5.08VSEE38 pKa = 4.13WAVEE42 pKa = 3.63NWLRR46 pKa = 11.84FVDD49 pKa = 4.92LFTIPVARR57 pKa = 11.84RR58 pKa = 11.84SQAFWVHH65 pKa = 4.16QKK67 pKa = 9.21MRR69 pKa = 11.84FIGHH73 pKa = 6.04TPSDD77 pKa = 3.88HH78 pKa = 7.31VSLLEE83 pKa = 3.93IGFMAAVAYY92 pKa = 10.49CMVSTAYY99 pKa = 10.33AQISPLVKK107 pKa = 9.58VIRR110 pKa = 11.84RR111 pKa = 11.84TFVVYY116 pKa = 8.28TQQKK120 pKa = 7.24TGIYY124 pKa = 8.9SFEE127 pKa = 4.65PEE129 pKa = 3.6RR130 pKa = 11.84MMPGSSFEE138 pKa = 4.13ASSIPSFQAEE148 pKa = 4.24VIIRR152 pKa = 11.84RR153 pKa = 11.84DD154 pKa = 3.45GIYY157 pKa = 10.1YY158 pKa = 9.94QAGQGFLTSAGKK170 pKa = 10.43YY171 pKa = 9.15FVTAYY176 pKa = 10.15HH177 pKa = 5.39VVEE180 pKa = 4.55GSEE183 pKa = 4.21DD184 pKa = 3.69VQLKK188 pKa = 5.76TLKK191 pKa = 10.84GEE193 pKa = 4.46VYY195 pKa = 10.23VSPDD199 pKa = 3.42RR200 pKa = 11.84FCAMDD205 pKa = 4.25GDD207 pKa = 4.52LVYY210 pKa = 10.39MEE212 pKa = 4.83ITPQEE217 pKa = 3.9RR218 pKa = 11.84SALNLSSAKK227 pKa = 10.48LMSTAVGKK235 pKa = 10.97GSGLFVQAVAGGQKK249 pKa = 10.97SMGLLDD255 pKa = 3.2KK256 pKa = 11.13HH257 pKa = 5.83EE258 pKa = 4.4AFGYY262 pKa = 7.91VTYY265 pKa = 10.47KK266 pKa = 10.8GSTIKK271 pKa = 10.69GFSGSPYY278 pKa = 9.11YY279 pKa = 10.51VGKK282 pKa = 8.11TVYY285 pKa = 9.79GLHH288 pKa = 6.63IGGSTEE294 pKa = 3.72NLGYY298 pKa = 10.44DD299 pKa = 3.16AAYY302 pKa = 9.99ISMQLSSFEE311 pKa = 4.15EE312 pKa = 4.76DD313 pKa = 3.04SSKK316 pKa = 10.49MFIEE320 pKa = 4.07QALARR325 pKa = 11.84GDD327 pKa = 4.26EE328 pKa = 4.4INFMRR333 pKa = 11.84SPYY336 pKa = 10.41DD337 pKa = 3.24PEE339 pKa = 4.31EE340 pKa = 4.33YY341 pKa = 10.05FVEE344 pKa = 4.61HH345 pKa = 6.07NGKK348 pKa = 9.57YY349 pKa = 9.9YY350 pKa = 11.08VMEE353 pKa = 4.57EE354 pKa = 4.18EE355 pKa = 5.65DD356 pKa = 4.85LDD358 pKa = 4.18DD359 pKa = 3.9LRR361 pKa = 11.84KK362 pKa = 9.92VKK364 pKa = 10.47RR365 pKa = 11.84GSVKK369 pKa = 10.33QGILQPVYY377 pKa = 10.11EE378 pKa = 4.78RR379 pKa = 11.84EE380 pKa = 4.03SAAAGSPRR388 pKa = 11.84VDD390 pKa = 3.77EE391 pKa = 5.32DD392 pKa = 3.96LPAAPRR398 pKa = 11.84TALVFQDD405 pKa = 3.26QEE407 pKa = 4.28NLVRR411 pKa = 11.84APAVVAGARR420 pKa = 11.84GEE422 pKa = 4.17VLASQEE428 pKa = 4.07IVRR431 pKa = 11.84DD432 pKa = 3.71PLLAGPRR439 pKa = 11.84LAGLLPRR446 pKa = 11.84EE447 pKa = 4.22SLEE450 pKa = 4.37SKK452 pKa = 9.31ATAGPSQIPAVPSAPLDD469 pKa = 3.68STVKK473 pKa = 10.46SIPKK477 pKa = 8.87SARR480 pKa = 11.84ARR482 pKa = 11.84RR483 pKa = 11.84LRR485 pKa = 11.84NLRR488 pKa = 11.84QKK490 pKa = 10.07IARR493 pKa = 11.84LEE495 pKa = 4.15QPSGLTRR502 pKa = 11.84RR503 pKa = 11.84GKK505 pKa = 10.53SILDD509 pKa = 3.24RR510 pKa = 11.84RR511 pKa = 11.84VASMTFSRR519 pKa = 11.84ASSPAQQ525 pKa = 3.16

Molecular weight: 58.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

872

347

525

436.0

48.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.028 ± 0.833

1.261 ± 0.621

4.702 ± 0.46

5.619 ± 0.6

4.931 ± 0.324

7.683 ± 0.401

1.72 ± 0.177

4.243 ± 0.467

4.817 ± 0.221

9.06 ± 0.611

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.555 ± 0.114

1.95 ± 0.555

4.931 ± 0.191

4.243 ± 0.124

6.193 ± 0.427

8.601 ± 1.688

4.472 ± 0.082

8.486 ± 0.266

1.835 ± 0.966

3.67 ± 0.469
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski