Red clover vein mosaic virus
Average proteome isoelectric point is 7.34
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C0L9F0|C0L9F0_9VIRU Movement protein TGB2 OS=Red clover vein mosaic virus OX=590403 PE=3 SV=1
MM1 pKa = 7.86 DD2 pKa = 3.51 VLFNVLEE9 pKa = 4.2 LCGFTRR15 pKa = 11.84 SSIRR19 pKa = 11.84 VGNPVVILAVPGAGKK34 pKa = 7.56 TTFLRR39 pKa = 11.84 RR40 pKa = 11.84 LLLEE44 pKa = 3.73 DD45 pKa = 3.64 TRR47 pKa = 11.84 FIGCTFGIPDD57 pKa = 3.54 PHH59 pKa = 7.2 GVTGRR64 pKa = 11.84 HH65 pKa = 5.72 ILDD68 pKa = 3.63 ARR70 pKa = 11.84 TLGPVEE76 pKa = 4.1 EE77 pKa = 4.75 GKK79 pKa = 10.79 LVLVDD84 pKa = 3.3 EE85 pKa = 5.08 FQRR88 pKa = 11.84 GDD90 pKa = 3.66 YY91 pKa = 10.22 KK92 pKa = 11.3 ALNPYY97 pKa = 10.13 AILGDD102 pKa = 3.6 VAQFCLAPNLVIEE115 pKa = 4.65 ANWFKK120 pKa = 10.92 SSSHH124 pKa = 6.02 RR125 pKa = 11.84 VPSVVCDD132 pKa = 3.9 LLNTLGFSITGSSVGEE148 pKa = 3.62 LHH150 pKa = 7.05 ILGLFEE156 pKa = 5.52 SDD158 pKa = 3.92 LRR160 pKa = 11.84 GTVITYY166 pKa = 10.33 DD167 pKa = 3.69 PEE169 pKa = 4.92 ICQLLTDD176 pKa = 4.43 HH177 pKa = 6.51 TCEE180 pKa = 4.07 HH181 pKa = 6.84 TSLEE185 pKa = 4.02 ACAGVEE191 pKa = 4.0 FEE193 pKa = 5.06 EE194 pKa = 5.69 VSLLLNGPVIPAGDD208 pKa = 3.36 RR209 pKa = 11.84 AKK211 pKa = 10.62 FYY213 pKa = 10.91 LAATRR218 pKa = 11.84 ASRR221 pKa = 11.84 VLNIFLPIISEE232 pKa = 3.96 LSIAGHH238 pKa = 6.68 ASDD241 pKa = 4.11 STTT244 pKa = 3.06
Molecular weight: 26.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.962
IPC2_protein 5.029
IPC_protein 4.965
Toseland 4.94
ProMoST 5.118
Dawson 4.978
Bjellqvist 5.105
Wikipedia 4.863
Rodwell 4.902
Grimsley 4.863
Solomon 4.978
Lehninger 4.94
Nozaki 5.105
DTASelect 5.258
Thurlkill 4.952
EMBOSS 4.914
Sillero 5.181
Patrickios 3.783
IPC_peptide 4.991
IPC2_peptide 5.181
IPC2.peptide.svr19 5.109
Protein with the highest isoelectric point:
>tr|C0L9F1|C0L9F1_9VIRU 7 kDa protein OS=Red clover vein mosaic virus OX=590403 PE=3 SV=1
MM1 pKa = 7.95 PLTPPPDD8 pKa = 3.36 YY9 pKa = 11.06 SKK11 pKa = 11.32 AVLSICVGLGLAVVLFTATRR31 pKa = 11.84 STLPHH36 pKa = 6.47 VGDD39 pKa = 4.85 NIHH42 pKa = 6.57 HH43 pKa = 6.79 LPHH46 pKa = 6.51 GGLYY50 pKa = 10.16 KK51 pKa = 10.93 DD52 pKa = 3.73 GTKK55 pKa = 10.39 SIAFGRR61 pKa = 11.84 PGGGNLTRR69 pKa = 11.84 DD70 pKa = 3.5 TFVRR74 pKa = 11.84 PPAPLFLVIGLVLLITWVSIRR95 pKa = 11.84 DD96 pKa = 3.45 SRR98 pKa = 11.84 VNRR101 pKa = 11.84 GHH103 pKa = 6.63 CSCVRR108 pKa = 11.84 SS109 pKa = 3.65
Molecular weight: 11.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.255
IPC2_protein 9.209
IPC_protein 9.487
Toseland 9.911
ProMoST 9.677
Dawson 10.131
Bjellqvist 9.911
Wikipedia 10.321
Rodwell 10.35
Grimsley 10.204
Solomon 10.218
Lehninger 10.204
Nozaki 10.101
DTASelect 9.853
Thurlkill 9.999
EMBOSS 10.321
Sillero 10.101
Patrickios 10.16
IPC_peptide 10.218
IPC2_peptide 9.238
IPC2.peptide.svr19 8.013
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2763
65
1932
460.5
51.62
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.406 ± 0.732
2.895 ± 0.378
5.212 ± 0.276
6.659 ± 1.045
6.587 ± 0.987
6.877 ± 0.581
2.208 ± 0.358
5.284 ± 0.463
6.117 ± 1.074
10.858 ± 0.926
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.81 ± 0.339
4.126 ± 0.296
4.488 ± 0.656
2.353 ± 0.341
5.537 ± 0.6
7.673 ± 0.432
4.85 ± 0.68
6.515 ± 0.91
1.05 ± 0.194
2.497 ± 0.395
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here