Red clover vein mosaic virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Betaflexiviridae; Quinvirinae; Carlavirus

Average proteome isoelectric point is 7.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|C0L9F0|C0L9F0_9VIRU Movement protein TGB2 OS=Red clover vein mosaic virus OX=590403 PE=3 SV=1
MM1 pKa = 7.86DD2 pKa = 3.51VLFNVLEE9 pKa = 4.2LCGFTRR15 pKa = 11.84SSIRR19 pKa = 11.84VGNPVVILAVPGAGKK34 pKa = 7.56TTFLRR39 pKa = 11.84RR40 pKa = 11.84LLLEE44 pKa = 3.73DD45 pKa = 3.64TRR47 pKa = 11.84FIGCTFGIPDD57 pKa = 3.54PHH59 pKa = 7.2GVTGRR64 pKa = 11.84HH65 pKa = 5.72ILDD68 pKa = 3.63ARR70 pKa = 11.84TLGPVEE76 pKa = 4.1EE77 pKa = 4.75GKK79 pKa = 10.79LVLVDD84 pKa = 3.3EE85 pKa = 5.08FQRR88 pKa = 11.84GDD90 pKa = 3.66YY91 pKa = 10.22KK92 pKa = 11.3ALNPYY97 pKa = 10.13AILGDD102 pKa = 3.6VAQFCLAPNLVIEE115 pKa = 4.65ANWFKK120 pKa = 10.92SSSHH124 pKa = 6.02RR125 pKa = 11.84VPSVVCDD132 pKa = 3.9LLNTLGFSITGSSVGEE148 pKa = 3.62LHH150 pKa = 7.05ILGLFEE156 pKa = 5.52SDD158 pKa = 3.92LRR160 pKa = 11.84GTVITYY166 pKa = 10.33DD167 pKa = 3.69PEE169 pKa = 4.92ICQLLTDD176 pKa = 4.43HH177 pKa = 6.51TCEE180 pKa = 4.07HH181 pKa = 6.84TSLEE185 pKa = 4.02ACAGVEE191 pKa = 4.0FEE193 pKa = 5.06EE194 pKa = 5.69VSLLLNGPVIPAGDD208 pKa = 3.36RR209 pKa = 11.84AKK211 pKa = 10.62FYY213 pKa = 10.91LAATRR218 pKa = 11.84ASRR221 pKa = 11.84VLNIFLPIISEE232 pKa = 3.96LSIAGHH238 pKa = 6.68ASDD241 pKa = 4.11STTT244 pKa = 3.06

Molecular weight:
26.38 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|C0L9F1|C0L9F1_9VIRU 7 kDa protein OS=Red clover vein mosaic virus OX=590403 PE=3 SV=1
MM1 pKa = 7.95PLTPPPDD8 pKa = 3.36YY9 pKa = 11.06SKK11 pKa = 11.32AVLSICVGLGLAVVLFTATRR31 pKa = 11.84STLPHH36 pKa = 6.47VGDD39 pKa = 4.85NIHH42 pKa = 6.57HH43 pKa = 6.79LPHH46 pKa = 6.51GGLYY50 pKa = 10.16KK51 pKa = 10.93DD52 pKa = 3.73GTKK55 pKa = 10.39SIAFGRR61 pKa = 11.84PGGGNLTRR69 pKa = 11.84DD70 pKa = 3.5TFVRR74 pKa = 11.84PPAPLFLVIGLVLLITWVSIRR95 pKa = 11.84DD96 pKa = 3.45SRR98 pKa = 11.84VNRR101 pKa = 11.84GHH103 pKa = 6.63CSCVRR108 pKa = 11.84SS109 pKa = 3.65

Molecular weight:
11.67 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2763

65

1932

460.5

51.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.406 ± 0.732

2.895 ± 0.378

5.212 ± 0.276

6.659 ± 1.045

6.587 ± 0.987

6.877 ± 0.581

2.208 ± 0.358

5.284 ± 0.463

6.117 ± 1.074

10.858 ± 0.926

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.81 ± 0.339

4.126 ± 0.296

4.488 ± 0.656

2.353 ± 0.341

5.537 ± 0.6

7.673 ± 0.432

4.85 ± 0.68

6.515 ± 0.91

1.05 ± 0.194

2.497 ± 0.395

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski