Chilo suppressalis (Asiatic rice borer moth)
Average proteome isoelectric point is 6.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 15558 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3S2NGZ1|A0A3S2NGZ1_CHISP ABC transporter domain-containing protein (Fragment) OS=Chilo suppressalis OX=168631 GN=evm_015542 PE=4 SV=1
MM1 pKa = 7.48 CRR3 pKa = 11.84 NSSFSSILDD12 pKa = 3.48 LALILDD18 pKa = 4.38 NNDD21 pKa = 3.56 NDD23 pKa = 5.0 DD24 pKa = 3.74 STSNRR29 pKa = 11.84 NTHH32 pKa = 6.76 DD33 pKa = 3.6 EE34 pKa = 4.13 NSNLEE39 pKa = 4.24 PDD41 pKa = 4.57 DD42 pKa = 4.53 PCDD45 pKa = 3.31 TAMSSPLPPGARR57 pKa = 11.84 NIKK60 pKa = 10.25 EE61 pKa = 3.99 DD62 pKa = 3.6 MAEE65 pKa = 3.72 VWNDD69 pKa = 3.16 VFNNNYY75 pKa = 9.97 PIDD78 pKa = 4.12 FSQYY82 pKa = 9.6 QLQATYY88 pKa = 8.43 DD89 pKa = 3.56 TTFPCC94 pKa = 5.41
Molecular weight: 10.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.731
IPC2_protein 3.63
IPC_protein 3.605
Toseland 3.389
ProMoST 3.821
Dawson 3.63
Bjellqvist 3.795
Wikipedia 3.617
Rodwell 3.439
Grimsley 3.3
Solomon 3.605
Lehninger 3.567
Nozaki 3.757
DTASelect 4.037
Thurlkill 3.478
EMBOSS 3.617
Sillero 3.745
Patrickios 0.769
IPC_peptide 3.592
IPC2_peptide 3.706
IPC2.peptide.svr19 3.683
Protein with the highest isoelectric point:
>tr|A0A437BMJ8|A0A437BMJ8_CHISP Uncharacterized protein (Fragment) OS=Chilo suppressalis OX=168631 GN=evm_003678 PE=4 SV=1
MM1 pKa = 7.18 PQNGLRR7 pKa = 11.84 GRR9 pKa = 11.84 GTEE12 pKa = 3.85 IEE14 pKa = 4.23 GLAVAVAVATSMQSFVVLRR33 pKa = 11.84 CDD35 pKa = 3.82 CVIDD39 pKa = 4.81 LLVLLWAHH47 pKa = 5.9 NQLKK51 pKa = 9.78 LAKK54 pKa = 9.64 SYY56 pKa = 11.48 YY57 pKa = 10.63 GLLKK61 pKa = 10.82 DD62 pKa = 4.18 GIGRR66 pKa = 11.84 LGHH69 pKa = 6.65 DD70 pKa = 3.85 PPRR73 pKa = 11.84 GLSADD78 pKa = 2.84 WWVLTTADD86 pKa = 3.41 AAGTNGLTSGGFVVIATLGRR106 pKa = 11.84 RR107 pKa = 11.84 VGDD110 pKa = 3.12 RR111 pKa = 11.84 SVAMEE116 pKa = 4.5 LKK118 pKa = 10.22 EE119 pKa = 4.03 KK120 pKa = 9.99 TLLSVMTPMTPPLRR134 pKa = 11.84 LLRR137 pKa = 11.84 HH138 pKa = 5.92 PWDD141 pKa = 3.67 KK142 pKa = 11.82 GEE144 pKa = 3.76 WCYY147 pKa = 11.59 SRR149 pKa = 11.84 LEE151 pKa = 4.15 HH152 pKa = 6.06 TRR154 pKa = 11.84 HH155 pKa = 5.49 FFVKK159 pKa = 10.55 FMLITTQSNLRR170 pKa = 11.84 IFTSIACAFPNLRR183 pKa = 11.84 GTNIRR188 pKa = 11.84 TSPLRR193 pKa = 11.84 VRR195 pKa = 11.84 KK196 pKa = 9.97 LIRR199 pKa = 11.84 CHH201 pKa = 6.49 SVSTKK206 pKa = 9.91 LVRR209 pKa = 11.84 RR210 pKa = 11.84 AQKK213 pKa = 9.52 TEE215 pKa = 3.84 TPHH218 pKa = 6.76 PISYY222 pKa = 9.36 MIFLEE227 pKa = 4.11 NWQKK231 pKa = 10.91 RR232 pKa = 11.84 ALLLRR237 pKa = 11.84 LYY239 pKa = 10.49 CGVAPCIGGCSRR251 pKa = 11.84 DD252 pKa = 3.88 DD253 pKa = 3.67 FTKK256 pKa = 10.52 KK257 pKa = 10.25 SYY259 pKa = 10.5 IGIGVAIQILPGPHH273 pKa = 5.52 RR274 pKa = 11.84 TLVTEE279 pKa = 4.47 GTLLSHH285 pKa = 7.08 TLTISVARR293 pKa = 11.84 AAGLPHH299 pKa = 6.55 TVLHH303 pKa = 6.39 HH304 pKa = 5.3 QLAVPVRR311 pKa = 11.84 LVRR314 pKa = 11.84 RR315 pKa = 11.84 TPEE318 pKa = 4.75 DD319 pKa = 3.04 IRR321 pKa = 11.84 KK322 pKa = 9.71 SSPILRR328 pKa = 11.84 RR329 pKa = 11.84 AEE331 pKa = 4.04 RR332 pKa = 11.84 VDD334 pKa = 3.28 TDD336 pKa = 3.58 VVRR339 pKa = 11.84 ARR341 pKa = 11.84 LLRR344 pKa = 11.84 EE345 pKa = 3.86 LSGEE349 pKa = 4.05 ALVGAVQAAALARR362 pKa = 11.84 HH363 pKa = 6.33 AGALHH368 pKa = 6.99 LSRR371 pKa = 11.84 HH372 pKa = 5.12 SRR374 pKa = 11.84 SGRR377 pKa = 11.84 DD378 pKa = 3.34 GTADD382 pKa = 3.21 AAQYY386 pKa = 10.51 RR387 pKa = 11.84 AVHH390 pKa = 6.52 DD391 pKa = 4.16 PGIRR395 pKa = 11.84 DD396 pKa = 3.47 HH397 pKa = 6.62 VAAGVAASQAPLPNVAACRR416 pKa = 11.84 RR417 pKa = 11.84 LL418 pKa = 3.29
Molecular weight: 45.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.355
IPC2_protein 9.56
IPC_protein 10.438
Toseland 10.657
ProMoST 10.409
Dawson 10.76
Bjellqvist 10.496
Wikipedia 10.979
Rodwell 10.877
Grimsley 10.804
Solomon 10.891
Lehninger 10.862
Nozaki 10.672
DTASelect 10.482
Thurlkill 10.672
EMBOSS 11.082
Sillero 10.701
Patrickios 10.57
IPC_peptide 10.906
IPC2_peptide 9.75
IPC2.peptide.svr19 8.583
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
15558
0
15558
7277707
52
18336
467.8
52.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.107 ± 0.03
2.131 ± 0.036
5.474 ± 0.016
6.481 ± 0.03
3.508 ± 0.016
5.602 ± 0.028
2.584 ± 0.013
5.294 ± 0.021
6.315 ± 0.032
9.067 ± 0.029
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.227 ± 0.01
4.828 ± 0.023
5.549 ± 0.033
3.912 ± 0.02
5.833 ± 0.025
7.674 ± 0.022
5.796 ± 0.023
6.297 ± 0.015
1.14 ± 0.008
3.18 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here