Sinorhizobium fredii (strain NBRC 101917 / NGR234)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium; Sinorhizobium fredii group; Sinorhizobium fredii

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6273 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C3MB35|C3MB35_SINFN Penicillin-binding protein OS=Sinorhizobium fredii (strain NBRC 101917 / NGR234) OX=394 GN=NGR_c12470 PE=3 SV=1
MM1 pKa = 7.31GVTVSVADD9 pKa = 3.57SHH11 pKa = 6.61GLDD14 pKa = 3.15MRR16 pKa = 11.84EE17 pKa = 3.7IDD19 pKa = 4.88FSSLLYY25 pKa = 10.29ADD27 pKa = 4.09SYY29 pKa = 11.35SRR31 pKa = 11.84SSSIFSAHH39 pKa = 5.77TGNWTDD45 pKa = 3.24QFRR48 pKa = 11.84GSGFKK53 pKa = 10.53YY54 pKa = 10.4DD55 pKa = 3.51GNGYY59 pKa = 6.8PTAGTVTSYY68 pKa = 11.44AILSGGSRR76 pKa = 11.84MVLISGFSIAAAKK89 pKa = 8.17ITSAAKK95 pKa = 9.58TGSLSDD101 pKa = 3.88DD102 pKa = 3.52LAIIRR107 pKa = 11.84DD108 pKa = 3.83ALAGNDD114 pKa = 3.61NLNGGEE120 pKa = 4.02FANYY124 pKa = 9.73LRR126 pKa = 11.84GYY128 pKa = 9.7AGNDD132 pKa = 3.52VISGGQYY139 pKa = 10.6SDD141 pKa = 5.54DD142 pKa = 4.18LFGDD146 pKa = 4.26DD147 pKa = 5.45GDD149 pKa = 4.85DD150 pKa = 5.11KK151 pKa = 11.46ISGDD155 pKa = 4.04YY156 pKa = 11.04GWDD159 pKa = 3.15NLYY162 pKa = 11.04GGNGNDD168 pKa = 3.98TLNGGAGSDD177 pKa = 3.97EE178 pKa = 4.45LSGGAGSDD186 pKa = 3.23TASYY190 pKa = 10.48VGASEE195 pKa = 4.89GVVASLKK202 pKa = 10.68NLASNTNDD210 pKa = 3.12ASGDD214 pKa = 3.84SYY216 pKa = 12.02SSIEE220 pKa = 4.04NLTGTSYY227 pKa = 12.05ADD229 pKa = 3.53TLEE232 pKa = 4.45GNANANSLTGGNGNDD247 pKa = 3.41RR248 pKa = 11.84LIGRR252 pKa = 11.84AGGDD256 pKa = 3.57VLNGGAGTDD265 pKa = 3.08SAYY268 pKa = 10.95YY269 pKa = 9.67YY270 pKa = 10.38GATVGVIANLSNAAANTNDD289 pKa = 3.57AAGDD293 pKa = 3.84VYY295 pKa = 11.45SSIEE299 pKa = 4.08SLVGSSYY306 pKa = 10.27TDD308 pKa = 2.9RR309 pKa = 11.84LFGNGVANTLIGNGGHH325 pKa = 7.21DD326 pKa = 3.95SLVGYY331 pKa = 10.22AGNDD335 pKa = 3.5GLYY338 pKa = 10.42GGAGADD344 pKa = 3.42RR345 pKa = 11.84LFGGTGADD353 pKa = 2.76RR354 pKa = 11.84FIFKK358 pKa = 8.23EE359 pKa = 4.44TTEE362 pKa = 4.34STSSVTDD369 pKa = 3.7SIFDD373 pKa = 3.8FLVSEE378 pKa = 4.26QDD380 pKa = 3.53RR381 pKa = 11.84IDD383 pKa = 3.4VSGIDD388 pKa = 3.36ARR390 pKa = 11.84LSVGGDD396 pKa = 3.11QAFVFIGTAAFSGDD410 pKa = 3.38GSEE413 pKa = 4.81LRR415 pKa = 11.84YY416 pKa = 10.17EE417 pKa = 4.28RR418 pKa = 11.84LASDD422 pKa = 3.82TYY424 pKa = 10.93IYY426 pKa = 11.17ADD428 pKa = 3.26VDD430 pKa = 3.57GDD432 pKa = 3.85QVADD436 pKa = 3.83LTIHH440 pKa = 6.97LDD442 pKa = 3.59DD443 pKa = 6.55AITLTSGHH451 pKa = 6.88FILL454 pKa = 6.01

Molecular weight: 46.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C3MFA3|C3MFA3_SINFN Uncharacterized protein OS=Sinorhizobium fredii (strain NBRC 101917 / NGR234) OX=394 GN=NGR_c01370 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.46GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.27GGQKK29 pKa = 9.61VLAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6273

0

6273

1950178

28

2870

310.9

33.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.144 ± 0.043

0.882 ± 0.01

5.563 ± 0.026

6.004 ± 0.023

3.861 ± 0.02

8.381 ± 0.027

2.074 ± 0.017

5.476 ± 0.022

3.542 ± 0.025

10.002 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.476 ± 0.015

2.696 ± 0.019

4.937 ± 0.023

2.997 ± 0.02

7.141 ± 0.033

5.685 ± 0.025

5.201 ± 0.023

7.397 ± 0.027

1.275 ± 0.015

2.266 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski