Amino acid dipepetide frequency for Escherichia phage phiKT

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.782AlaAla: 14.782 ± 2.526
0.89AlaCys: 0.89 ± 0.371
6.055AlaAsp: 6.055 ± 0.837
7.124AlaGlu: 7.124 ± 0.736
3.295AlaPhe: 3.295 ± 0.647
8.103AlaGly: 8.103 ± 0.884
1.247AlaHis: 1.247 ± 0.299
5.076AlaIle: 5.076 ± 0.564
4.63AlaLys: 4.63 ± 0.684
8.014AlaLeu: 8.014 ± 0.861
2.76AlaMet: 2.76 ± 0.546
5.432AlaAsn: 5.432 ± 1.534
3.384AlaPro: 3.384 ± 1.236
5.076AlaGln: 5.076 ± 0.946
3.473AlaArg: 3.473 ± 0.604
5.699AlaSer: 5.699 ± 1.006
6.322AlaThr: 6.322 ± 1.507
6.768AlaVal: 6.768 ± 0.856
1.425AlaTrp: 1.425 ± 0.271
3.206AlaTyr: 3.206 ± 0.587
0.0AlaXaa: 0.0 ± 0.0
Cys
0.801CysAla: 0.801 ± 0.313
0.0CysCys: 0.0 ± 0.0
0.356CysAsp: 0.356 ± 0.196
0.623CysGlu: 0.623 ± 0.277
0.267CysPhe: 0.267 ± 0.124
0.089CysGly: 0.089 ± 0.089
0.178CysHis: 0.178 ± 0.126
0.89CysIle: 0.89 ± 0.291
0.534CysLys: 0.534 ± 0.237
0.98CysLeu: 0.98 ± 0.377
0.267CysMet: 0.267 ± 0.162
0.356CysAsn: 0.356 ± 0.175
0.445CysPro: 0.445 ± 0.228
0.178CysGln: 0.178 ± 0.122
0.356CysArg: 0.356 ± 0.188
0.623CysSer: 0.623 ± 0.275
0.712CysThr: 0.712 ± 0.361
0.534CysVal: 0.534 ± 0.175
0.0CysTrp: 0.0 ± 0.0
0.178CysTyr: 0.178 ± 0.127
0.0CysXaa: 0.0 ± 0.0
Asp
5.788AspAla: 5.788 ± 0.8
0.623AspCys: 0.623 ± 0.238
4.185AspAsp: 4.185 ± 0.723
3.562AspGlu: 3.562 ± 0.598
2.939AspPhe: 2.939 ± 0.361
5.521AspGly: 5.521 ± 0.82
0.712AspHis: 0.712 ± 0.325
3.117AspIle: 3.117 ± 0.495
3.473AspLys: 3.473 ± 0.481
4.809AspLeu: 4.809 ± 0.862
1.692AspMet: 1.692 ± 0.307
1.781AspAsn: 1.781 ± 0.365
3.295AspPro: 3.295 ± 0.5
2.048AspGln: 2.048 ± 0.496
2.493AspArg: 2.493 ± 0.455
3.829AspSer: 3.829 ± 0.701
3.651AspThr: 3.651 ± 0.498
4.185AspVal: 4.185 ± 0.48
1.425AspTrp: 1.425 ± 0.32
2.226AspTyr: 2.226 ± 0.596
0.0AspXaa: 0.0 ± 0.0
Glu
5.966GluAla: 5.966 ± 0.919
0.623GluCys: 0.623 ± 0.275
2.939GluAsp: 2.939 ± 0.559
3.295GluGlu: 3.295 ± 0.779
2.76GluPhe: 2.76 ± 0.468
1.959GluGly: 1.959 ± 0.466
1.692GluHis: 1.692 ± 0.42
3.384GluIle: 3.384 ± 0.662
3.74GluLys: 3.74 ± 0.795
4.452GluLeu: 4.452 ± 0.523
1.603GluMet: 1.603 ± 0.415
2.048GluAsn: 2.048 ± 0.3
1.603GluPro: 1.603 ± 0.379
3.295GluGln: 3.295 ± 0.526
3.829GluArg: 3.829 ± 0.542
3.651GluSer: 3.651 ± 0.574
3.295GluThr: 3.295 ± 0.574
3.829GluVal: 3.829 ± 0.484
0.98GluTrp: 0.98 ± 0.199
2.493GluTyr: 2.493 ± 0.462
0.0GluXaa: 0.0 ± 0.0
Phe
2.404PheAla: 2.404 ± 0.491
0.267PheCys: 0.267 ± 0.134
2.939PheAsp: 2.939 ± 0.545
1.87PheGlu: 1.87 ± 0.296
1.069PhePhe: 1.069 ± 0.436
3.295PheGly: 3.295 ± 0.522
0.445PheHis: 0.445 ± 0.148
2.315PheIle: 2.315 ± 0.37
1.425PheLys: 1.425 ± 0.308
3.117PheLeu: 3.117 ± 0.564
1.069PheMet: 1.069 ± 0.364
0.89PheAsn: 0.89 ± 0.262
1.247PhePro: 1.247 ± 0.307
1.959PheGln: 1.959 ± 0.255
2.404PheArg: 2.404 ± 0.387
2.315PheSer: 2.315 ± 0.451
1.692PheThr: 1.692 ± 0.325
1.959PheVal: 1.959 ± 0.399
0.178PheTrp: 0.178 ± 0.126
1.425PheTyr: 1.425 ± 0.353
0.0PheXaa: 0.0 ± 0.0
Gly
5.966GlyAla: 5.966 ± 0.624
0.445GlyCys: 0.445 ± 0.244
6.589GlyAsp: 6.589 ± 0.54
2.76GlyGlu: 2.76 ± 0.31
2.671GlyPhe: 2.671 ± 0.504
6.679GlyGly: 6.679 ± 0.867
1.781GlyHis: 1.781 ± 0.435
3.829GlyIle: 3.829 ± 0.618
3.918GlyLys: 3.918 ± 0.634
5.076GlyLeu: 5.076 ± 0.813
2.226GlyMet: 2.226 ± 0.448
4.185GlyAsn: 4.185 ± 0.505
1.692GlyPro: 1.692 ± 0.403
2.85GlyGln: 2.85 ± 0.472
4.096GlyArg: 4.096 ± 0.538
4.452GlySer: 4.452 ± 0.787
6.679GlyThr: 6.679 ± 0.737
6.411GlyVal: 6.411 ± 1.032
1.069GlyTrp: 1.069 ± 0.381
3.117GlyTyr: 3.117 ± 0.569
0.0GlyXaa: 0.0 ± 0.0
His
1.336HisAla: 1.336 ± 0.297
0.267HisCys: 0.267 ± 0.162
1.336HisAsp: 1.336 ± 0.442
1.247HisGlu: 1.247 ± 0.334
0.712HisPhe: 0.712 ± 0.261
2.137HisGly: 2.137 ± 0.623
0.623HisHis: 0.623 ± 0.317
0.356HisIle: 0.356 ± 0.267
0.445HisLys: 0.445 ± 0.196
1.425HisLeu: 1.425 ± 0.274
0.712HisMet: 0.712 ± 0.239
0.712HisAsn: 0.712 ± 0.23
1.158HisPro: 1.158 ± 0.361
0.623HisGln: 0.623 ± 0.269
0.98HisArg: 0.98 ± 0.275
0.98HisSer: 0.98 ± 0.319
1.425HisThr: 1.425 ± 0.374
0.89HisVal: 0.89 ± 0.323
0.445HisTrp: 0.445 ± 0.183
0.534HisTyr: 0.534 ± 0.157
0.0HisXaa: 0.0 ± 0.0
Ile
4.72IleAla: 4.72 ± 0.607
0.89IleCys: 0.89 ± 0.301
3.829IleAsp: 3.829 ± 0.537
2.671IleGlu: 2.671 ± 0.752
1.069IlePhe: 1.069 ± 0.292
3.028IleGly: 3.028 ± 0.503
0.712IleHis: 0.712 ± 0.209
2.048IleIle: 2.048 ± 0.528
2.939IleLys: 2.939 ± 0.512
3.295IleLeu: 3.295 ± 0.671
1.247IleMet: 1.247 ± 0.402
2.493IleAsn: 2.493 ± 0.426
2.582IlePro: 2.582 ± 0.57
1.514IleGln: 1.514 ± 0.329
2.582IleArg: 2.582 ± 0.451
3.562IleSer: 3.562 ± 0.647
3.651IleThr: 3.651 ± 0.555
3.384IleVal: 3.384 ± 0.533
0.801IleTrp: 0.801 ± 0.261
1.87IleTyr: 1.87 ± 0.392
0.0IleXaa: 0.0 ± 0.0
Lys
6.768LysAla: 6.768 ± 0.742
0.267LysCys: 0.267 ± 0.147
3.473LysAsp: 3.473 ± 0.562
3.028LysGlu: 3.028 ± 0.578
2.315LysPhe: 2.315 ± 0.467
3.028LysGly: 3.028 ± 0.47
1.069LysHis: 1.069 ± 0.366
2.048LysIle: 2.048 ± 0.423
4.541LysLys: 4.541 ± 0.945
4.987LysLeu: 4.987 ± 0.921
2.226LysMet: 2.226 ± 0.369
2.226LysAsn: 2.226 ± 0.345
2.137LysPro: 2.137 ± 0.37
1.959LysGln: 1.959 ± 0.301
3.295LysArg: 3.295 ± 0.627
2.85LysSer: 2.85 ± 0.349
2.76LysThr: 2.76 ± 0.468
3.384LysVal: 3.384 ± 0.458
1.247LysTrp: 1.247 ± 0.281
1.692LysTyr: 1.692 ± 0.344
0.0LysXaa: 0.0 ± 0.0
Leu
6.946LeuAla: 6.946 ± 0.63
0.712LeuCys: 0.712 ± 0.234
5.343LeuAsp: 5.343 ± 0.632
4.63LeuGlu: 4.63 ± 0.749
2.315LeuPhe: 2.315 ± 0.475
5.788LeuGly: 5.788 ± 1.076
1.959LeuHis: 1.959 ± 0.51
4.541LeuIle: 4.541 ± 0.867
4.363LeuLys: 4.363 ± 0.689
6.322LeuLeu: 6.322 ± 0.803
2.048LeuMet: 2.048 ± 0.364
4.096LeuAsn: 4.096 ± 0.526
2.85LeuPro: 2.85 ± 0.465
3.117LeuGln: 3.117 ± 0.576
2.671LeuArg: 2.671 ± 0.315
7.124LeuSer: 7.124 ± 0.821
4.185LeuThr: 4.185 ± 0.699
5.61LeuVal: 5.61 ± 0.943
0.801LeuTrp: 0.801 ± 0.299
2.404LeuTyr: 2.404 ± 0.412
0.0LeuXaa: 0.0 ± 0.0
Met
2.85MetAla: 2.85 ± 0.487
0.089MetCys: 0.089 ± 0.074
1.692MetAsp: 1.692 ± 0.453
0.98MetGlu: 0.98 ± 0.355
1.247MetPhe: 1.247 ± 0.269
1.959MetGly: 1.959 ± 0.513
0.267MetHis: 0.267 ± 0.136
1.247MetIle: 1.247 ± 0.382
1.514MetLys: 1.514 ± 0.289
3.117MetLeu: 3.117 ± 0.504
0.623MetMet: 0.623 ± 0.278
1.336MetAsn: 1.336 ± 0.317
1.069MetPro: 1.069 ± 0.291
1.781MetGln: 1.781 ± 0.492
2.493MetArg: 2.493 ± 0.674
1.87MetSer: 1.87 ± 0.406
1.425MetThr: 1.425 ± 0.491
2.76MetVal: 2.76 ± 0.514
0.534MetTrp: 0.534 ± 0.21
1.069MetTyr: 1.069 ± 0.393
0.0MetXaa: 0.0 ± 0.0
Asn
4.63AsnAla: 4.63 ± 0.828
0.178AsnCys: 0.178 ± 0.135
1.247AsnAsp: 1.247 ± 0.25
2.671AsnGlu: 2.671 ± 0.371
1.514AsnPhe: 1.514 ± 0.379
3.562AsnGly: 3.562 ± 0.638
0.98AsnHis: 0.98 ± 0.289
2.76AsnIle: 2.76 ± 0.415
1.781AsnLys: 1.781 ± 0.314
3.562AsnLeu: 3.562 ± 0.61
1.603AsnMet: 1.603 ± 0.383
2.85AsnAsn: 2.85 ± 0.597
2.226AsnPro: 2.226 ± 0.424
1.425AsnGln: 1.425 ± 0.364
2.493AsnArg: 2.493 ± 0.527
2.404AsnSer: 2.404 ± 0.457
4.096AsnThr: 4.096 ± 1.014
1.959AsnVal: 1.959 ± 0.45
0.801AsnTrp: 0.801 ± 0.237
1.425AsnTyr: 1.425 ± 0.383
0.0AsnXaa: 0.0 ± 0.0
Pro
4.185ProAla: 4.185 ± 1.054
0.178ProCys: 0.178 ± 0.125
2.85ProAsp: 2.85 ± 0.511
3.473ProGlu: 3.473 ± 0.593
1.247ProPhe: 1.247 ± 0.305
2.85ProGly: 2.85 ± 0.6
0.445ProHis: 0.445 ± 0.17
1.247ProIle: 1.247 ± 0.269
1.692ProLys: 1.692 ± 0.422
2.939ProLeu: 2.939 ± 0.434
1.158ProMet: 1.158 ± 0.285
2.048ProAsn: 2.048 ± 0.429
1.425ProPro: 1.425 ± 0.455
2.315ProGln: 2.315 ± 0.562
1.247ProArg: 1.247 ± 0.397
2.404ProSer: 2.404 ± 0.553
2.582ProThr: 2.582 ± 0.416
3.918ProVal: 3.918 ± 0.826
0.267ProTrp: 0.267 ± 0.164
1.514ProTyr: 1.514 ± 0.329
0.0ProXaa: 0.0 ± 0.0
Gln
5.521GlnAla: 5.521 ± 1.049
0.089GlnCys: 0.089 ± 0.093
1.425GlnAsp: 1.425 ± 0.346
2.226GlnGlu: 2.226 ± 0.447
1.425GlnPhe: 1.425 ± 0.271
2.76GlnGly: 2.76 ± 0.449
0.801GlnHis: 0.801 ± 0.269
1.959GlnIle: 1.959 ± 0.459
2.048GlnLys: 2.048 ± 0.435
3.829GlnLeu: 3.829 ± 0.591
1.781GlnMet: 1.781 ± 0.343
1.514GlnAsn: 1.514 ± 0.496
2.048GlnPro: 2.048 ± 0.442
3.829GlnGln: 3.829 ± 0.927
2.939GlnArg: 2.939 ± 0.576
3.028GlnSer: 3.028 ± 0.631
2.137GlnThr: 2.137 ± 0.388
3.295GlnVal: 3.295 ± 0.581
0.356GlnTrp: 0.356 ± 0.182
1.603GlnTyr: 1.603 ± 0.416
0.0GlnXaa: 0.0 ± 0.0
Arg
4.274ArgAla: 4.274 ± 0.723
1.158ArgCys: 1.158 ± 0.335
3.295ArgAsp: 3.295 ± 0.445
4.185ArgGlu: 4.185 ± 0.614
2.404ArgPhe: 2.404 ± 0.462
4.452ArgGly: 4.452 ± 0.555
1.069ArgHis: 1.069 ± 0.32
3.295ArgIle: 3.295 ± 0.45
2.85ArgLys: 2.85 ± 0.608
3.829ArgLeu: 3.829 ± 0.566
2.493ArgMet: 2.493 ± 0.399
1.603ArgAsn: 1.603 ± 0.416
2.493ArgPro: 2.493 ± 0.405
1.603ArgGln: 1.603 ± 0.384
3.206ArgArg: 3.206 ± 0.8
1.959ArgSer: 1.959 ± 0.528
2.226ArgThr: 2.226 ± 0.421
3.028ArgVal: 3.028 ± 0.586
0.98ArgTrp: 0.98 ± 0.382
1.781ArgTyr: 1.781 ± 0.348
0.0ArgXaa: 0.0 ± 0.0
Ser
6.857SerAla: 6.857 ± 1.068
0.178SerCys: 0.178 ± 0.113
4.096SerAsp: 4.096 ± 0.752
3.117SerGlu: 3.117 ± 0.728
1.603SerPhe: 1.603 ± 0.31
5.165SerGly: 5.165 ± 0.677
0.712SerHis: 0.712 ± 0.257
3.117SerIle: 3.117 ± 0.458
3.918SerLys: 3.918 ± 0.648
4.987SerLeu: 4.987 ± 0.672
1.514SerMet: 1.514 ± 0.341
2.493SerAsn: 2.493 ± 0.362
2.939SerPro: 2.939 ± 0.612
2.315SerGln: 2.315 ± 0.746
3.562SerArg: 3.562 ± 0.574
4.007SerSer: 4.007 ± 0.676
4.898SerThr: 4.898 ± 1.032
4.987SerVal: 4.987 ± 0.758
0.712SerTrp: 0.712 ± 0.248
2.226SerTyr: 2.226 ± 0.444
0.0SerXaa: 0.0 ± 0.0
Thr
8.103ThrAla: 8.103 ± 2.315
0.356ThrCys: 0.356 ± 0.186
4.274ThrAsp: 4.274 ± 0.789
2.939ThrGlu: 2.939 ± 0.506
1.959ThrPhe: 1.959 ± 0.34
6.679ThrGly: 6.679 ± 0.725
1.425ThrHis: 1.425 ± 0.306
2.582ThrIle: 2.582 ± 0.508
4.72ThrLys: 4.72 ± 0.544
4.63ThrLeu: 4.63 ± 0.729
1.603ThrMet: 1.603 ± 0.362
2.582ThrAsn: 2.582 ± 0.507
3.384ThrPro: 3.384 ± 0.561
2.315ThrGln: 2.315 ± 0.467
3.206ThrArg: 3.206 ± 0.488
4.274ThrSer: 4.274 ± 0.479
5.076ThrThr: 5.076 ± 0.977
3.384ThrVal: 3.384 ± 0.683
0.445ThrTrp: 0.445 ± 0.223
1.87ThrTyr: 1.87 ± 0.436
0.0ThrXaa: 0.0 ± 0.0
Val
6.857ValAla: 6.857 ± 0.664
0.534ValCys: 0.534 ± 0.253
3.117ValAsp: 3.117 ± 0.483
4.63ValGlu: 4.63 ± 0.48
1.603ValPhe: 1.603 ± 0.291
5.966ValGly: 5.966 ± 0.837
1.514ValHis: 1.514 ± 0.412
2.85ValIle: 2.85 ± 0.428
3.651ValLys: 3.651 ± 0.486
4.007ValLeu: 4.007 ± 0.574
1.959ValMet: 1.959 ± 0.37
3.117ValAsn: 3.117 ± 0.364
2.582ValPro: 2.582 ± 0.421
4.096ValGln: 4.096 ± 0.753
4.452ValArg: 4.452 ± 0.854
4.987ValSer: 4.987 ± 0.663
5.254ValThr: 5.254 ± 0.698
5.254ValVal: 5.254 ± 0.815
1.069ValTrp: 1.069 ± 0.244
2.137ValTyr: 2.137 ± 0.471
0.0ValXaa: 0.0 ± 0.0
Trp
0.445TrpAla: 0.445 ± 0.213
0.267TrpCys: 0.267 ± 0.175
1.069TrpAsp: 1.069 ± 0.261
0.801TrpGlu: 0.801 ± 0.292
0.445TrpPhe: 0.445 ± 0.193
0.623TrpGly: 0.623 ± 0.25
0.178TrpHis: 0.178 ± 0.125
0.445TrpIle: 0.445 ± 0.175
0.801TrpLys: 0.801 ± 0.297
1.514TrpLeu: 1.514 ± 0.354
0.445TrpMet: 0.445 ± 0.149
0.623TrpAsn: 0.623 ± 0.275
0.445TrpPro: 0.445 ± 0.173
0.98TrpGln: 0.98 ± 0.258
0.712TrpArg: 0.712 ± 0.222
1.158TrpSer: 1.158 ± 0.357
0.89TrpThr: 0.89 ± 0.354
1.158TrpVal: 1.158 ± 0.304
0.267TrpTrp: 0.267 ± 0.16
0.89TrpTyr: 0.89 ± 0.312
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.562TyrAla: 3.562 ± 0.548
0.356TyrCys: 0.356 ± 0.186
1.247TyrAsp: 1.247 ± 0.289
1.603TyrGlu: 1.603 ± 0.449
1.425TyrPhe: 1.425 ± 0.397
2.85TyrGly: 2.85 ± 0.374
0.534TyrHis: 0.534 ± 0.16
1.781TyrIle: 1.781 ± 0.337
2.315TyrLys: 2.315 ± 0.343
2.939TyrLeu: 2.939 ± 0.652
0.801TyrMet: 0.801 ± 0.301
1.87TyrAsn: 1.87 ± 0.363
0.98TyrPro: 0.98 ± 0.326
1.336TyrGln: 1.336 ± 0.427
1.692TyrArg: 1.692 ± 0.444
2.226TyrSer: 2.226 ± 0.409
2.85TyrThr: 2.85 ± 0.519
2.939TyrVal: 2.939 ± 0.475
0.356TyrTrp: 0.356 ± 0.164
1.425TyrTyr: 1.425 ± 0.356
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 31 proteins (11231 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski