Gilvibacter sp. SZ-19
Average proteome isoelectric point is 6.12
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2809 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Y0MPK8|A0A1Y0MPK8_9FLAO Uncharacterized protein OS=Gilvibacter sp. SZ-19 OX=754429 GN=BTO09_05445 PE=4 SV=1
MM1 pKa = 6.85 NTNSVQRR8 pKa = 11.84 IIVDD12 pKa = 3.6 YY13 pKa = 11.1 QKK15 pKa = 10.37 MKK17 pKa = 10.48 PEE19 pKa = 3.99 VMQQLLEE26 pKa = 4.09 QYY28 pKa = 10.26 PDD30 pKa = 4.17 GYY32 pKa = 10.44 DD33 pKa = 3.32 TDD35 pKa = 3.81 QLIQFKK41 pKa = 9.98 NAQGLWVYY49 pKa = 10.18 CLEE52 pKa = 4.32 VKK54 pKa = 9.92 TPEE57 pKa = 3.66 TVYY60 pKa = 10.57 LVKK63 pKa = 10.69 VSRR66 pKa = 11.84 KK67 pKa = 9.3 LDD69 pKa = 3.62 EE70 pKa = 5.43 AIDD73 pKa = 3.65 RR74 pKa = 11.84 ALAEE78 pKa = 4.68 DD79 pKa = 5.73 DD80 pKa = 3.88 EE81 pKa = 6.69 DD82 pKa = 4.18 EE83 pKa = 4.94 DD84 pKa = 4.91 YY85 pKa = 11.3 SYY87 pKa = 11.63 QSDD90 pKa = 4.66 DD91 pKa = 3.99 IEE93 pKa = 6.17 DD94 pKa = 3.63 SADD97 pKa = 3.33 YY98 pKa = 11.14 SDD100 pKa = 4.49 YY101 pKa = 11.62 SDD103 pKa = 3.92 DD104 pKa = 4.43 FEE106 pKa = 4.66 YY107 pKa = 11.3 AA108 pKa = 3.19
Molecular weight: 12.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.7
IPC2_protein 3.757
IPC_protein 3.757
Toseland 3.528
ProMoST 3.923
Dawson 3.745
Bjellqvist 3.91
Wikipedia 3.694
Rodwell 3.579
Grimsley 3.439
Solomon 3.732
Lehninger 3.694
Nozaki 3.872
DTASelect 4.101
Thurlkill 3.592
EMBOSS 3.706
Sillero 3.872
Patrickios 0.998
IPC_peptide 3.732
IPC2_peptide 3.846
IPC2.peptide.svr19 3.786
Protein with the highest isoelectric point:
>tr|A0A1Y0MKY4|A0A1Y0MKY4_9FLAO DUF4129 domain-containing protein OS=Gilvibacter sp. SZ-19 OX=754429 GN=BTO09_01050 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.59 RR3 pKa = 11.84 TFQPSKK9 pKa = 9.13 RR10 pKa = 11.84 KK11 pKa = 9.48 RR12 pKa = 11.84 RR13 pKa = 11.84 NKK15 pKa = 9.49 HH16 pKa = 3.94 GFRR19 pKa = 11.84 EE20 pKa = 4.18 RR21 pKa = 11.84 MASVNGRR28 pKa = 11.84 KK29 pKa = 9.18 VLASRR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.04 GRR39 pKa = 11.84 KK40 pKa = 8.7 KK41 pKa = 9.83 LTVSSEE47 pKa = 3.9 PRR49 pKa = 11.84 HH50 pKa = 5.77 KK51 pKa = 10.61 KK52 pKa = 9.84
Molecular weight: 6.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.33
IPC2_protein 10.716
IPC_protein 12.266
Toseland 12.442
ProMoST 12.925
Dawson 12.442
Bjellqvist 12.427
Wikipedia 12.91
Rodwell 12.281
Grimsley 12.486
Solomon 12.939
Lehninger 12.837
Nozaki 12.442
DTASelect 12.427
Thurlkill 12.442
EMBOSS 12.939
Sillero 12.442
Patrickios 12.003
IPC_peptide 12.939
IPC2_peptide 11.915
IPC2.peptide.svr19 9.051
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2809
0
2809
944669
38
2532
336.3
37.64
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.912 ± 0.046
0.756 ± 0.015
5.978 ± 0.046
6.431 ± 0.049
5.022 ± 0.038
6.938 ± 0.045
1.78 ± 0.025
6.726 ± 0.04
6.169 ± 0.062
9.706 ± 0.061
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.294 ± 0.025
5.112 ± 0.044
3.786 ± 0.028
3.916 ± 0.03
4.048 ± 0.03
6.085 ± 0.037
5.671 ± 0.055
6.571 ± 0.036
1.133 ± 0.017
3.968 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here