Pea stem necrosis virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Procedovirinae; Gammacarmovirus

Average proteome isoelectric point is 7.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q7T4K0|Q7T4K0_9TOMB p6 product OS=Pea stem necrosis virus OX=199361 GN=p6 PE=4 SV=1
MM1 pKa = 7.6GNLPNNSDD9 pKa = 4.07DD10 pKa = 3.45FWVNKK15 pKa = 8.67VRR17 pKa = 11.84GWWRR21 pKa = 11.84RR22 pKa = 11.84ASRR25 pKa = 11.84EE26 pKa = 4.24SYY28 pKa = 10.6SSCGCCDD35 pKa = 3.25RR36 pKa = 11.84CPWKK40 pKa = 10.7APCYY44 pKa = 9.89HH45 pKa = 6.39SRR47 pKa = 11.84NSNTVVTHH55 pKa = 6.29RR56 pKa = 11.84EE57 pKa = 3.93YY58 pKa = 10.77VTDD61 pKa = 3.62VSNSSGVFVNNSSGSSSVFRR81 pKa = 11.84INPSNTRR88 pKa = 11.84VFNWLSSIAGSYY100 pKa = 10.83DD101 pKa = 3.11SYY103 pKa = 11.54KK104 pKa = 8.89FTRR107 pKa = 11.84VRR109 pKa = 11.84FTYY112 pKa = 10.41VPYY115 pKa = 10.63AGSNTPGRR123 pKa = 11.84LYY125 pKa = 10.81LGWDD129 pKa = 3.55ADD131 pKa = 4.2SQDD134 pKa = 3.69VIQPDD139 pKa = 3.79RR140 pKa = 11.84ASLANFSPTSEE151 pKa = 4.07DD152 pKa = 3.91SVWTDD157 pKa = 2.7SYY159 pKa = 11.8LVIPVDD165 pKa = 3.82RR166 pKa = 11.84EE167 pKa = 3.73WRR169 pKa = 11.84FVDD172 pKa = 3.4DD173 pKa = 4.04TNISSRR179 pKa = 11.84KK180 pKa = 9.68LVDD183 pKa = 4.02LGQLVLATWGGVDD196 pKa = 3.2NTVCGEE202 pKa = 4.18VYY204 pKa = 10.38VEE206 pKa = 3.9YY207 pKa = 10.46SVEE210 pKa = 3.79LRR212 pKa = 11.84QPQPPSGFVQVGRR225 pKa = 11.84VDD227 pKa = 3.33VPGILTFTGPAFVPASDD244 pKa = 4.96LSISDD249 pKa = 3.34TSFSFNLNTAGQYY262 pKa = 10.97ALSIDD267 pKa = 4.02LQATTSGTLTVAGNCTLLGVVKK289 pKa = 10.44SQFASGVGIYY299 pKa = 9.05MCIVRR304 pKa = 11.84STGGPNSAASISIGTLTGLSRR325 pKa = 11.84VQFFLSRR332 pKa = 11.84GTTSAGIRR340 pKa = 11.84VTCISSEE347 pKa = 3.97RR348 pKa = 11.84HH349 pKa = 5.2LSS351 pKa = 3.4

Molecular weight: 38.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q7T4K3|Q7T4K3_9TOMB RNA-directed RNA polymerase OS=Pea stem necrosis virus OX=199361 GN=p84 PE=4 SV=2
MM1 pKa = 7.67SIRR4 pKa = 11.84MKK6 pKa = 10.79FGLALVAGCASVMYY20 pKa = 9.11VAYY23 pKa = 10.0RR24 pKa = 11.84RR25 pKa = 11.84RR26 pKa = 11.84VVRR29 pKa = 11.84GKK31 pKa = 9.11MLPRR35 pKa = 11.84NLQIEE40 pKa = 4.29NSRR43 pKa = 11.84VVLKK47 pKa = 10.64VLNSQSDD54 pKa = 3.97GVDD57 pKa = 3.93FPEE60 pKa = 4.66EE61 pKa = 4.13DD62 pKa = 4.0SVDD65 pKa = 3.39SSGPVILIGSVEE77 pKa = 4.46VKK79 pKa = 10.54LPAPVTVKK87 pKa = 10.5RR88 pKa = 11.84KK89 pKa = 9.46PKK91 pKa = 9.99AKK93 pKa = 9.39HH94 pKa = 5.83KK95 pKa = 10.73SMPFITKK102 pKa = 10.14LINAAKK108 pKa = 9.76VHH110 pKa = 6.44FDD112 pKa = 3.53GVPKK116 pKa = 9.16PTEE119 pKa = 4.24SNHH122 pKa = 4.85MAVTRR127 pKa = 11.84FIKK130 pKa = 10.06DD131 pKa = 3.13YY132 pKa = 10.31CKK134 pKa = 10.66EE135 pKa = 4.0HH136 pKa = 7.01GVDD139 pKa = 3.91DD140 pKa = 4.04NQTRR144 pKa = 11.84RR145 pKa = 11.84VCAIAGPLILSPDD158 pKa = 3.26RR159 pKa = 11.84TDD161 pKa = 2.76IMSRR165 pKa = 11.84AFLYY169 pKa = 10.81GPEE172 pKa = 4.22LSKK175 pKa = 10.95QRR177 pKa = 11.84AEE179 pKa = 4.06YY180 pKa = 10.1AAAASTISWFDD191 pKa = 4.26AIVAAPLSCRR201 pKa = 11.84AWRR204 pKa = 11.84RR205 pKa = 11.84AYY207 pKa = 9.98LALMGYY213 pKa = 8.4PDD215 pKa = 3.59SVGYY219 pKa = 10.55QMVKK223 pKa = 10.58

Molecular weight: 24.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1444

57

750

288.8

32.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.202 ± 0.985

2.424 ± 0.328

4.778 ± 0.375

4.155 ± 0.736

4.294 ± 0.395

7.202 ± 0.591

2.008 ± 0.446

5.471 ± 0.658

5.194 ± 1.306

7.825 ± 0.889

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.355 ± 0.78

4.432 ± 0.662

4.848 ± 0.397

3.324 ± 0.347

6.925 ± 0.319

9.418 ± 1.689

4.224 ± 1.148

9.003 ± 0.562

1.385 ± 0.375

3.463 ± 0.092
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski