Cardiospermum yellow leaf curl betasatellite
Average proteome isoelectric point is 4.71
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B0JEU4|B0JEU4_9VIRU Beta-C1 protein OS=Cardiospermum yellow leaf curl betasatellite OX=497865 GN=beta-C1 PE=4 SV=1
MM1 pKa = 8.22 PITKK5 pKa = 8.61 KK6 pKa = 9.35 TSSGKK11 pKa = 10.13 LLQLSVNVFDD21 pKa = 4.96 SKK23 pKa = 11.09 FIVLLHH29 pKa = 5.76 ITDD32 pKa = 3.83 TKK34 pKa = 11.02 SPQIQLQKK42 pKa = 10.64 IKK44 pKa = 10.74 LPYY47 pKa = 10.16 SFNSDD52 pKa = 3.57 DD53 pKa = 4.39 LLINVTGLEE62 pKa = 4.19 EE63 pKa = 4.56 EE64 pKa = 4.57 LMQHH68 pKa = 7.0 LSLICDD74 pKa = 3.17 EE75 pKa = 4.73 HH76 pKa = 8.3 VPSKK80 pKa = 11.23 VYY82 pKa = 10.13 TDD84 pKa = 3.81 YY85 pKa = 11.3 LISAIDD91 pKa = 3.48 ILILEE96 pKa = 4.55 NSQEE100 pKa = 4.06 LGLQVLEE107 pKa = 4.36 PCVVTITYY115 pKa = 7.57 TVV117 pKa = 2.85
Molecular weight: 13.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.713
IPC2_protein 4.838
IPC_protein 4.698
Toseland 4.584
ProMoST 4.825
Dawson 4.673
Bjellqvist 4.813
Wikipedia 4.558
Rodwell 4.571
Grimsley 4.495
Solomon 4.673
Lehninger 4.622
Nozaki 4.787
DTASelect 4.952
Thurlkill 4.596
EMBOSS 4.584
Sillero 4.838
Patrickios 3.605
IPC_peptide 4.673
IPC2_peptide 4.838
IPC2.peptide.svr19 4.781
Protein with the highest isoelectric point:
>tr|B0JEU4|B0JEU4_9VIRU Beta-C1 protein OS=Cardiospermum yellow leaf curl betasatellite OX=497865 GN=beta-C1 PE=4 SV=1
MM1 pKa = 8.22 PITKK5 pKa = 8.61 KK6 pKa = 9.35 TSSGKK11 pKa = 10.13 LLQLSVNVFDD21 pKa = 4.96 SKK23 pKa = 11.09 FIVLLHH29 pKa = 5.76 ITDD32 pKa = 3.83 TKK34 pKa = 11.02 SPQIQLQKK42 pKa = 10.64 IKK44 pKa = 10.74 LPYY47 pKa = 10.16 SFNSDD52 pKa = 3.57 DD53 pKa = 4.39 LLINVTGLEE62 pKa = 4.19 EE63 pKa = 4.56 EE64 pKa = 4.57 LMQHH68 pKa = 7.0 LSLICDD74 pKa = 3.17 EE75 pKa = 4.73 HH76 pKa = 8.3 VPSKK80 pKa = 11.23 VYY82 pKa = 10.13 TDD84 pKa = 3.81 YY85 pKa = 11.3 LISAIDD91 pKa = 3.48 ILILEE96 pKa = 4.55 NSQEE100 pKa = 4.06 LGLQVLEE107 pKa = 4.36 PCVVTITYY115 pKa = 7.57 TVV117 pKa = 2.85
Molecular weight: 13.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.713
IPC2_protein 4.838
IPC_protein 4.698
Toseland 4.584
ProMoST 4.825
Dawson 4.673
Bjellqvist 4.813
Wikipedia 4.558
Rodwell 4.571
Grimsley 4.495
Solomon 4.673
Lehninger 4.622
Nozaki 4.787
DTASelect 4.952
Thurlkill 4.596
EMBOSS 4.584
Sillero 4.838
Patrickios 3.605
IPC_peptide 4.673
IPC2_peptide 4.838
IPC2.peptide.svr19 4.781
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
117
117
117
117.0
13.18
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
0.855 ± 0.0
1.709 ± 0.0
5.983 ± 0.0
5.983 ± 0.0
2.564 ± 0.0
2.564 ± 0.0
2.564 ± 0.0
10.256 ± 0.0
6.838 ± 0.0
16.239 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.709 ± 0.0
3.419 ± 0.0
4.274 ± 0.0
5.983 ± 0.0
0.0 ± 0.0
9.402 ± 0.0
7.692 ± 0.0
8.547 ± 0.0
0.0 ± 0.0
3.419 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here