Mycobacterium phage Baee

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bclasvirinae; Acadianvirus; Mycobacterium virus Baee

Average proteome isoelectric point is 5.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 96 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F6YQN7|A0A0F6YQN7_9CAUD Uncharacterized protein OS=Mycobacterium phage Baee OX=1647306 GN=45 PE=4 SV=1
MM1 pKa = 7.56PVYY4 pKa = 10.5IGDD7 pKa = 3.81QPIDD11 pKa = 4.96DD12 pKa = 4.19ILGITDD18 pKa = 3.91ADD20 pKa = 4.57DD21 pKa = 3.72YY22 pKa = 11.72DD23 pKa = 3.93AVYY26 pKa = 10.52LGSDD30 pKa = 3.72QVWPPIEE37 pKa = 3.95FPYY40 pKa = 10.52IVIDD44 pKa = 4.41SNITDD49 pKa = 3.63AEE51 pKa = 4.44VPEE54 pKa = 4.66GTTGCWVSLHH64 pKa = 6.53GGGEE68 pKa = 3.96AGGKK72 pKa = 9.62GGHH75 pKa = 6.39EE76 pKa = 5.24DD77 pKa = 3.35GTTATGSASGGARR90 pKa = 11.84GAGGAVVDD98 pKa = 4.24RR99 pKa = 11.84FFIYY103 pKa = 10.78AEE105 pKa = 4.63DD106 pKa = 4.09LPSTLWSLIKK116 pKa = 11.01GNGGASNGIDD126 pKa = 3.23GTASRR131 pKa = 11.84FLCGSVDD138 pKa = 3.14ILAGGGAAQVGGTASVTGLPTDD160 pKa = 3.98YY161 pKa = 10.72YY162 pKa = 11.44APKK165 pKa = 9.64LYY167 pKa = 10.91NGASSGNATGAGAAGGSGGGSSWTGDD193 pKa = 3.32NTPNPSNGSSGGTSTPATDD212 pKa = 4.04QYY214 pKa = 10.98WPAGDD219 pKa = 4.53GGDD222 pKa = 3.79GGNGHH227 pKa = 6.88GSGAGHH233 pKa = 7.3HH234 pKa = 5.92YY235 pKa = 10.92ASGGGGGGGGGAYY248 pKa = 10.26DD249 pKa = 4.46GLNGSNGTQNAGGNGGRR266 pKa = 11.84GGDD269 pKa = 3.21GRR271 pKa = 11.84VEE273 pKa = 4.14VEE275 pKa = 3.97WVNTHH280 pKa = 5.52VPRR283 pKa = 11.84DD284 pKa = 3.74RR285 pKa = 11.84TWQFGAGAWSWTCPAWAQTGWKK307 pKa = 9.79VDD309 pKa = 3.76IIEE312 pKa = 4.2WAGGKK317 pKa = 9.95AGANGGSTSAGNGGLAGATNSQTLTLGTDD346 pKa = 2.91IALGSTLSGNVGTGGASNGANGGNTTCTQLGHH378 pKa = 6.23NVLGATGNTGAQDD391 pKa = 4.0GQASSPITIGGKK403 pKa = 7.94TYY405 pKa = 10.33PGGPGGTSGTSSSAGQDD422 pKa = 3.06GSAPGGGGEE431 pKa = 4.54GGGSLFFVGLAGGKK445 pKa = 10.22GGDD448 pKa = 3.19GRR450 pKa = 11.84VIIRR454 pKa = 11.84LRR456 pKa = 11.84EE457 pKa = 3.81VV458 pKa = 2.83

Molecular weight: 43.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F6YRY9|A0A0F6YRY9_9CAUD Uncharacterized protein OS=Mycobacterium phage Baee OX=1647306 GN=82 PE=4 SV=1
MM1 pKa = 7.24TVVCSGPQPGSKK13 pKa = 9.42RR14 pKa = 11.84ASNRR18 pKa = 11.84TSDD21 pKa = 3.35RR22 pKa = 11.84DD23 pKa = 3.66TRR25 pKa = 11.84ALLAAIEE32 pKa = 4.11AAGGEE37 pKa = 4.28LKK39 pKa = 10.33PCRR42 pKa = 11.84SRR44 pKa = 11.84TGHH47 pKa = 5.52YY48 pKa = 9.75KK49 pKa = 10.25VYY51 pKa = 10.76LDD53 pKa = 3.68GRR55 pKa = 11.84LIGTIAGTPSEE66 pKa = 4.13YY67 pKa = 9.91RR68 pKa = 11.84SRR70 pKa = 11.84KK71 pKa = 9.28NDD73 pKa = 3.04IANLRR78 pKa = 11.84RR79 pKa = 11.84NGLQITSKK87 pKa = 10.45GAPITT92 pKa = 3.83

Molecular weight: 9.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

96

0

96

22616

34

2003

235.6

25.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.022 ± 0.375

1.008 ± 0.132

6.553 ± 0.258

5.912 ± 0.302

2.427 ± 0.099

9.763 ± 0.585

1.892 ± 0.152

4.696 ± 0.147

2.87 ± 0.149

8.025 ± 0.245

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.034 ± 0.117

2.892 ± 0.15

6.111 ± 0.237

3.197 ± 0.141

6.747 ± 0.388

5.412 ± 0.224

6.84 ± 0.211

7.296 ± 0.199

1.959 ± 0.155

2.343 ± 0.137
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski