Winogradskyella epiphytica

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Winogradskyella

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3007 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2V4WV60|A0A2V4WV60_9FLAO Uncharacterized protein OS=Winogradskyella epiphytica OX=262005 GN=DFQ11_10466 PE=4 SV=1
MM1 pKa = 7.5KK2 pKa = 10.55LKK4 pKa = 10.44FLRR7 pKa = 11.84LSLLLLAVFSVVISCKK23 pKa = 10.49DD24 pKa = 3.22DD25 pKa = 4.4DD26 pKa = 4.78DD27 pKa = 5.22FEE29 pKa = 5.15EE30 pKa = 4.81IPYY33 pKa = 10.22VLEE36 pKa = 5.69DD37 pKa = 3.49RR38 pKa = 11.84TEE40 pKa = 3.9QQAKK44 pKa = 10.65DD45 pKa = 3.55KK46 pKa = 11.23DD47 pKa = 3.93SLLSYY52 pKa = 10.94LSTHH56 pKa = 6.63YY57 pKa = 10.77YY58 pKa = 10.31NSAFFEE64 pKa = 4.39TGTNHH69 pKa = 7.44RR70 pKa = 11.84YY71 pKa = 9.15NDD73 pKa = 3.4IVITEE78 pKa = 4.25LPKK81 pKa = 10.96DD82 pKa = 3.46EE83 pKa = 4.62SGNYY87 pKa = 10.34LEE89 pKa = 5.88MPDD92 pKa = 4.43PEE94 pKa = 4.93NNTLLIEE101 pKa = 4.29AVEE104 pKa = 4.3TLTTEE109 pKa = 4.26YY110 pKa = 11.07KK111 pKa = 10.36DD112 pKa = 3.32VSYY115 pKa = 10.55EE116 pKa = 4.1YY117 pKa = 11.06YY118 pKa = 10.73VLNINQGGGEE128 pKa = 4.38SPNFTDD134 pKa = 3.66KK135 pKa = 11.04VRR137 pKa = 11.84VRR139 pKa = 11.84YY140 pKa = 9.93QGVSVGNQTVFDD152 pKa = 4.32AVSSPITLPLVGDD165 pKa = 4.5GFQTGGAIRR174 pKa = 11.84AWQLVIPTFKK184 pKa = 10.22TAVSYY189 pKa = 10.79SYY191 pKa = 11.38NDD193 pKa = 3.56GVVEE197 pKa = 3.96YY198 pKa = 10.58DD199 pKa = 3.79DD200 pKa = 5.64FGLGMMFVPSGLAYY214 pKa = 10.13FAGVNTGSAYY224 pKa = 10.83DD225 pKa = 3.52NLIFKK230 pKa = 10.62FEE232 pKa = 4.22LLQYY236 pKa = 10.36EE237 pKa = 4.77VVDD240 pKa = 4.35HH241 pKa = 7.41DD242 pKa = 4.9SDD244 pKa = 5.61GIPSWVEE251 pKa = 3.89DD252 pKa = 3.97LDD254 pKa = 5.86GDD256 pKa = 4.91LDD258 pKa = 4.15VSNDD262 pKa = 3.52DD263 pKa = 3.38TDD265 pKa = 5.06GNGFPNYY272 pKa = 9.79VDD274 pKa = 5.0RR275 pKa = 11.84DD276 pKa = 3.85DD277 pKa = 6.18DD278 pKa = 4.75GDD280 pKa = 4.05GVLTRR285 pKa = 11.84DD286 pKa = 3.45EE287 pKa = 5.71LIPTTYY293 pKa = 10.44TIDD296 pKa = 3.54LNAGEE301 pKa = 4.67EE302 pKa = 4.34EE303 pKa = 4.39PVLSEE308 pKa = 4.63GEE310 pKa = 4.3FEE312 pKa = 5.11VSRR315 pKa = 11.84DD316 pKa = 3.67LNNGILTIEE325 pKa = 4.37TVTIADD331 pKa = 3.96SNNDD335 pKa = 3.08GLADD339 pKa = 3.76YY340 pKa = 10.31LDD342 pKa = 4.17PNITINYY349 pKa = 9.07NEE351 pKa = 3.96

Molecular weight: 39.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2V4YH18|A0A2V4YH18_9FLAO 1-acyl-sn-glycerol-3-phosphate acyltransferase OS=Winogradskyella epiphytica OX=262005 GN=DFQ11_101645 PE=4 SV=1
MM1 pKa = 7.84PSGKK5 pKa = 9.32KK6 pKa = 9.59RR7 pKa = 11.84KK8 pKa = 7.05RR9 pKa = 11.84HH10 pKa = 5.11KK11 pKa = 10.59VATHH15 pKa = 5.21KK16 pKa = 10.3RR17 pKa = 11.84KK18 pKa = 9.62KK19 pKa = 9.04RR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84ANRR25 pKa = 11.84HH26 pKa = 4.5KK27 pKa = 10.78KK28 pKa = 9.37KK29 pKa = 10.83

Molecular weight: 3.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3007

0

3007

994994

29

2881

330.9

37.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.247 ± 0.04

0.738 ± 0.019

5.837 ± 0.039

6.752 ± 0.041

5.141 ± 0.035

6.156 ± 0.041

1.831 ± 0.023

8.047 ± 0.045

7.701 ± 0.068

9.307 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.204 ± 0.023

6.143 ± 0.048

3.305 ± 0.024

3.328 ± 0.025

3.439 ± 0.028

6.647 ± 0.044

5.882 ± 0.068

6.205 ± 0.036

0.978 ± 0.016

4.112 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski