Corynebacterium striatum
Average proteome isoelectric point is 5.97
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2642 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A376GVJ5|A0A376GVJ5_CORST 3-hydroxybutyryl-CoA dehydratase OS=Corynebacterium striatum OX=43770 GN=echA8_2 PE=3 SV=1
MM1 pKa = 7.24 LRR3 pKa = 11.84 SAVALLGAAVICLPLASCADD23 pKa = 4.06 DD24 pKa = 4.18 AAQDD28 pKa = 3.63 NSPAFDD34 pKa = 3.59 TVQNSATDD42 pKa = 3.62 NAPDD46 pKa = 4.4 DD47 pKa = 4.5 SEE49 pKa = 4.58 STDD52 pKa = 3.98 DD53 pKa = 4.52 PVSEE57 pKa = 4.65 PPAAGASPTLPAEE70 pKa = 4.5 SPAEE74 pKa = 4.01 EE75 pKa = 4.35 EE76 pKa = 4.3 DD77 pKa = 4.02 AEE79 pKa = 4.35 SCEE82 pKa = 4.58 TIDD85 pKa = 3.6 AHH87 pKa = 6.82 AAYY90 pKa = 9.46 EE91 pKa = 4.28 SGIGTIPPWNDD102 pKa = 3.19 NNWTLVDD109 pKa = 4.89 FSQFDD114 pKa = 3.75 PCAEE118 pKa = 4.64 LSWQTISIEE127 pKa = 4.29 RR128 pKa = 11.84 GTSSSPYY135 pKa = 10.05 HH136 pKa = 6.99 IMLFHH141 pKa = 6.96 KK142 pKa = 10.77 GEE144 pKa = 4.22 YY145 pKa = 10.12 LGTATAEE152 pKa = 4.25 PYY154 pKa = 11.12 GFFPTVEE161 pKa = 4.04 QVSSNEE167 pKa = 3.75 IAVTYY172 pKa = 8.91 HH173 pKa = 6.32 WPRR176 pKa = 11.84 EE177 pKa = 4.23 GEE179 pKa = 4.31 SNAGHH184 pKa = 6.41 TGEE187 pKa = 4.11 THH189 pKa = 7.41 AGFRR193 pKa = 11.84 WDD195 pKa = 4.92 DD196 pKa = 3.74 GQQKK200 pKa = 10.7 VIMSGDD206 pKa = 3.67 VPPGSS211 pKa = 3.56
Molecular weight: 22.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.745
IPC2_protein 3.859
IPC_protein 3.846
Toseland 3.643
ProMoST 3.986
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.732
Rodwell 3.668
Grimsley 3.554
Solomon 3.808
Lehninger 3.77
Nozaki 3.935
DTASelect 4.126
Thurlkill 3.681
EMBOSS 3.745
Sillero 3.961
Patrickios 0.807
IPC_peptide 3.808
IPC2_peptide 3.935
IPC2.peptide.svr19 3.849
Protein with the highest isoelectric point:
>tr|A0A0M2XBA3|A0A0M2XBA3_CORST Cold shock protein OS=Corynebacterium striatum OX=43770 GN=cspA PE=4 SV=1
MM1 pKa = 7.69 AKK3 pKa = 10.06 GKK5 pKa = 8.69 RR6 pKa = 11.84 TFQPNNRR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 ARR17 pKa = 11.84 KK18 pKa = 8.59 HH19 pKa = 4.58 GFRR22 pKa = 11.84 TRR24 pKa = 11.84 MSTRR28 pKa = 11.84 AGRR31 pKa = 11.84 AIVAARR37 pKa = 11.84 RR38 pKa = 11.84 KK39 pKa = 9.71 KK40 pKa = 10.51 GRR42 pKa = 11.84 AKK44 pKa = 10.73 LSAA47 pKa = 3.92
Molecular weight: 5.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.525
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.056
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.735
Grimsley 13.086
Solomon 13.539
Lehninger 13.451
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.554
Sillero 13.042
Patrickios 12.457
IPC_peptide 13.554
IPC2_peptide 12.53
IPC2.peptide.svr19 9.203
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2642
0
2642
831499
29
3042
314.7
34.14
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.793 ± 0.067
0.728 ± 0.015
5.82 ± 0.044
6.516 ± 0.054
3.473 ± 0.035
8.196 ± 0.045
2.14 ± 0.023
5.09 ± 0.034
3.694 ± 0.045
9.521 ± 0.059
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.284 ± 0.019
3.064 ± 0.027
4.929 ± 0.036
3.348 ± 0.029
5.924 ± 0.045
5.892 ± 0.033
5.914 ± 0.028
7.964 ± 0.047
1.382 ± 0.021
2.329 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here