Pacific flying fox associated multicomponent virus
Average proteome isoelectric point is 6.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A140CTS8|A0A140CTS8_9VIRU ATP-dependent helicase Rep OS=Pacific flying fox associated multicomponent virus OX=1796003 PE=3 SV=1
MM1 pKa = 7.91 PLRR4 pKa = 11.84 SRR6 pKa = 11.84 GWCYY10 pKa = 9.27 TVNNWTEE17 pKa = 3.79 NDD19 pKa = 3.44 EE20 pKa = 4.12 EE21 pKa = 4.43 LVAKK25 pKa = 10.71 LSEE28 pKa = 3.86 QTLYY32 pKa = 10.9 HH33 pKa = 5.86 IVARR37 pKa = 11.84 EE38 pKa = 3.62 IGEE41 pKa = 4.13 EE42 pKa = 4.36 GTIHH46 pKa = 6.18 LQGYY50 pKa = 9.69 CYY52 pKa = 10.46 FKK54 pKa = 10.86 NFKK57 pKa = 10.6 SLAQFKK63 pKa = 10.64 ALLPRR68 pKa = 11.84 AHH70 pKa = 7.34 LEE72 pKa = 4.34 TQKK75 pKa = 9.82 GTPQEE80 pKa = 4.0 ASDD83 pKa = 3.95 YY84 pKa = 10.13 CKK86 pKa = 10.59 KK87 pKa = 10.88 DD88 pKa = 2.76 GDD90 pKa = 4.23 YY91 pKa = 11.33 VEE93 pKa = 5.82 SGTLPMSQKK102 pKa = 10.6 RR103 pKa = 11.84 KK104 pKa = 9.2 GDD106 pKa = 3.37 LGRR109 pKa = 11.84 EE110 pKa = 3.99 YY111 pKa = 10.3 WEE113 pKa = 4.45 EE114 pKa = 3.71 QLSLAKK120 pKa = 9.99 KK121 pKa = 10.53 GKK123 pKa = 10.28 VEE125 pKa = 3.96 EE126 pKa = 4.97 CDD128 pKa = 3.32 PKK130 pKa = 11.25 LQITHH135 pKa = 6.92 FNALNAISARR145 pKa = 11.84 YY146 pKa = 9.8 APMPPDD152 pKa = 4.48 NEE154 pKa = 5.96 DD155 pKa = 3.65 IDD157 pKa = 4.46 NQWFYY162 pKa = 11.76 GPTGTGKK169 pKa = 9.94 SRR171 pKa = 11.84 KK172 pKa = 9.44 ARR174 pKa = 11.84 GDD176 pKa = 3.38 NPGCYY181 pKa = 10.14 LKK183 pKa = 9.94 MCNKK187 pKa = 8.77 WWDD190 pKa = 3.79 GYY192 pKa = 11.1 LGEE195 pKa = 4.3 EE196 pKa = 4.21 TVLIEE201 pKa = 5.74 DD202 pKa = 3.99 FDD204 pKa = 4.24 KK205 pKa = 10.78 KK206 pKa = 10.48 HH207 pKa = 5.86 EE208 pKa = 4.19 VLGHH212 pKa = 5.97 HH213 pKa = 6.53 LKK215 pKa = 9.94 IWADD219 pKa = 3.87 RR220 pKa = 11.84 YY221 pKa = 10.85 AFPAEE226 pKa = 4.25 VKK228 pKa = 10.22 GSKK231 pKa = 10.46 VNLRR235 pKa = 11.84 PKK237 pKa = 10.37 KK238 pKa = 10.51 IIVTSNFHH246 pKa = 6.67 PNEE249 pKa = 3.63 IWSDD253 pKa = 3.71 LSTLDD258 pKa = 4.14 PILRR262 pKa = 11.84 RR263 pKa = 11.84 FNVIKK268 pKa = 10.45 FSNPFSYY275 pKa = 10.67 LSPLRR280 pKa = 11.84 YY281 pKa = 9.44 LL282 pKa = 3.91
Molecular weight: 32.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.693
IPC2_protein 6.868
IPC_protein 6.956
Toseland 6.839
ProMoST 7.424
Dawson 7.571
Bjellqvist 7.614
Wikipedia 7.512
Rodwell 7.571
Grimsley 6.927
Solomon 7.644
Lehninger 7.673
Nozaki 7.892
DTASelect 7.746
Thurlkill 7.79
EMBOSS 7.849
Sillero 8.009
Patrickios 4.228
IPC_peptide 7.644
IPC2_peptide 7.132
IPC2.peptide.svr19 7.203
Protein with the highest isoelectric point:
>tr|A0A140CTS8|A0A140CTS8_9VIRU ATP-dependent helicase Rep OS=Pacific flying fox associated multicomponent virus OX=1796003 PE=3 SV=1
MM1 pKa = 7.91 PLRR4 pKa = 11.84 SRR6 pKa = 11.84 GWCYY10 pKa = 9.27 TVNNWTEE17 pKa = 3.79 NDD19 pKa = 3.44 EE20 pKa = 4.12 EE21 pKa = 4.43 LVAKK25 pKa = 10.71 LSEE28 pKa = 3.86 QTLYY32 pKa = 10.9 HH33 pKa = 5.86 IVARR37 pKa = 11.84 EE38 pKa = 3.62 IGEE41 pKa = 4.13 EE42 pKa = 4.36 GTIHH46 pKa = 6.18 LQGYY50 pKa = 9.69 CYY52 pKa = 10.46 FKK54 pKa = 10.86 NFKK57 pKa = 10.6 SLAQFKK63 pKa = 10.64 ALLPRR68 pKa = 11.84 AHH70 pKa = 7.34 LEE72 pKa = 4.34 TQKK75 pKa = 9.82 GTPQEE80 pKa = 4.0 ASDD83 pKa = 3.95 YY84 pKa = 10.13 CKK86 pKa = 10.59 KK87 pKa = 10.88 DD88 pKa = 2.76 GDD90 pKa = 4.23 YY91 pKa = 11.33 VEE93 pKa = 5.82 SGTLPMSQKK102 pKa = 10.6 RR103 pKa = 11.84 KK104 pKa = 9.2 GDD106 pKa = 3.37 LGRR109 pKa = 11.84 EE110 pKa = 3.99 YY111 pKa = 10.3 WEE113 pKa = 4.45 EE114 pKa = 3.71 QLSLAKK120 pKa = 9.99 KK121 pKa = 10.53 GKK123 pKa = 10.28 VEE125 pKa = 3.96 EE126 pKa = 4.97 CDD128 pKa = 3.32 PKK130 pKa = 11.25 LQITHH135 pKa = 6.92 FNALNAISARR145 pKa = 11.84 YY146 pKa = 9.8 APMPPDD152 pKa = 4.48 NEE154 pKa = 5.96 DD155 pKa = 3.65 IDD157 pKa = 4.46 NQWFYY162 pKa = 11.76 GPTGTGKK169 pKa = 9.94 SRR171 pKa = 11.84 KK172 pKa = 9.44 ARR174 pKa = 11.84 GDD176 pKa = 3.38 NPGCYY181 pKa = 10.14 LKK183 pKa = 9.94 MCNKK187 pKa = 8.77 WWDD190 pKa = 3.79 GYY192 pKa = 11.1 LGEE195 pKa = 4.3 EE196 pKa = 4.21 TVLIEE201 pKa = 5.74 DD202 pKa = 3.99 FDD204 pKa = 4.24 KK205 pKa = 10.78 KK206 pKa = 10.48 HH207 pKa = 5.86 EE208 pKa = 4.19 VLGHH212 pKa = 5.97 HH213 pKa = 6.53 LKK215 pKa = 9.94 IWADD219 pKa = 3.87 RR220 pKa = 11.84 YY221 pKa = 10.85 AFPAEE226 pKa = 4.25 VKK228 pKa = 10.22 GSKK231 pKa = 10.46 VNLRR235 pKa = 11.84 PKK237 pKa = 10.37 KK238 pKa = 10.51 IIVTSNFHH246 pKa = 6.67 PNEE249 pKa = 3.63 IWSDD253 pKa = 3.71 LSTLDD258 pKa = 4.14 PILRR262 pKa = 11.84 RR263 pKa = 11.84 FNVIKK268 pKa = 10.45 FSNPFSYY275 pKa = 10.67 LSPLRR280 pKa = 11.84 YY281 pKa = 9.44 LL282 pKa = 3.91
Molecular weight: 32.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.693
IPC2_protein 6.868
IPC_protein 6.956
Toseland 6.839
ProMoST 7.424
Dawson 7.571
Bjellqvist 7.614
Wikipedia 7.512
Rodwell 7.571
Grimsley 6.927
Solomon 7.644
Lehninger 7.673
Nozaki 7.892
DTASelect 7.746
Thurlkill 7.79
EMBOSS 7.849
Sillero 8.009
Patrickios 4.228
IPC_peptide 7.644
IPC2_peptide 7.132
IPC2.peptide.svr19 7.203
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
282
282
282
282.0
32.62
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.319 ± 0.0
2.128 ± 0.0
5.674 ± 0.0
7.801 ± 0.0
3.901 ± 0.0
6.738 ± 0.0
2.837 ± 0.0
4.61 ± 0.0
8.865 ± 0.0
9.574 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.418 ± 0.0
5.319 ± 0.0
5.674 ± 0.0
3.191 ± 0.0
4.965 ± 0.0
5.674 ± 0.0
4.61 ± 0.0
3.901 ± 0.0
2.837 ± 0.0
4.965 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here