Brazilian cedratvirus IHUMI
Average proteome isoelectric point is 6.19
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 533 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2R8FE23|A0A2R8FE23_9VIRU 5'-nucleotidase/apyrase OS=Brazilian cedratvirus IHUMI OX=2126980 GN=BRZCDTV_242 PE=4 SV=1
MM1 pKa = 7.77 SNTTYY6 pKa = 10.03 LTFNIARR13 pKa = 11.84 NGNLLEE19 pKa = 4.29 THH21 pKa = 6.55 TEE23 pKa = 4.06 TVLPQGKK30 pKa = 9.03 LVIDD34 pKa = 3.89 AQGTSFVSLRR44 pKa = 11.84 YY45 pKa = 9.69 NINSALIYY53 pKa = 10.81 ARR55 pKa = 11.84 DD56 pKa = 3.43 EE57 pKa = 4.13 LGILFTRR64 pKa = 11.84 PYY66 pKa = 10.13 KK67 pKa = 9.98 MVWRR71 pKa = 11.84 DD72 pKa = 2.95 HH73 pKa = 6.74 RR74 pKa = 11.84 GRR76 pKa = 11.84 LEE78 pKa = 3.8 EE79 pKa = 3.91 RR80 pKa = 11.84 VYY82 pKa = 11.27 SVDD85 pKa = 4.8 DD86 pKa = 3.79 NLPYY90 pKa = 10.26 HH91 pKa = 6.79 ASFVGDD97 pKa = 3.31 YY98 pKa = 10.54 SVEE101 pKa = 4.03 IVKK104 pKa = 10.86 LEE106 pKa = 3.84 VLEE109 pKa = 4.35 EE110 pKa = 4.2 EE111 pKa = 5.25 EE112 pKa = 4.54 NEE114 pKa = 4.05 FWNDD118 pKa = 3.13 DD119 pKa = 3.65 DD120 pKa = 5.5 VEE122 pKa = 4.22 EE123 pKa = 4.21 TEE125 pKa = 5.28 YY126 pKa = 11.43 YY127 pKa = 10.75 DD128 pKa = 4.73 EE129 pKa = 5.31 LDD131 pKa = 3.62 EE132 pKa = 4.9 YY133 pKa = 11.25 EE134 pKa = 4.23 EE135 pKa = 4.12 EE136 pKa = 4.89 PYY138 pKa = 10.98 LEE140 pKa = 4.27 EE141 pKa = 4.41 EE142 pKa = 4.8 EE143 pKa = 4.62 YY144 pKa = 11.38
Molecular weight: 17.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.875
IPC2_protein 4.126
IPC_protein 4.05
Toseland 3.897
ProMoST 4.164
Dawson 3.999
Bjellqvist 4.151
Wikipedia 3.859
Rodwell 3.897
Grimsley 3.808
Solomon 3.986
Lehninger 3.948
Nozaki 4.113
DTASelect 4.228
Thurlkill 3.897
EMBOSS 3.872
Sillero 4.164
Patrickios 1.914
IPC_peptide 3.999
IPC2_peptide 4.151
IPC2.peptide.svr19 4.064
Protein with the highest isoelectric point:
>tr|A0A2R8FEG9|A0A2R8FEG9_9VIRU Ribonucleoside-diphosphate reductase OS=Brazilian cedratvirus IHUMI OX=2126980 GN=BRZCDTV_310 PE=3 SV=1
LL1 pKa = 5.95 GTNRR5 pKa = 11.84 SLFTQHH11 pKa = 6.92 RR12 pKa = 11.84 SITFTKK18 pKa = 10.38 RR19 pKa = 11.84 EE20 pKa = 4.09 NFVLLCSLSLSLSLSSPNVRR40 pKa = 11.84 TSFSPKK46 pKa = 6.45 VTKK49 pKa = 10.67 VEE51 pKa = 4.1 EE52 pKa = 4.36 GTNRR56 pKa = 11.84 SPLLSLYY63 pKa = 7.95 TQGYY67 pKa = 4.9 QTRR70 pKa = 11.84 RR71 pKa = 11.84 GYY73 pKa = 10.83 QSFSFALSAPKK84 pKa = 8.72 VTKK87 pKa = 10.61 VEE89 pKa = 4.07 EE90 pKa = 4.85 GEE92 pKa = 4.33 VGTNRR97 pKa = 11.84 SLTNQTRR104 pKa = 11.84 RR105 pKa = 11.84 GYY107 pKa = 10.65 QSFSFALSSPRR118 pKa = 11.84 LLKK121 pKa = 10.81
Molecular weight: 13.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.328
IPC2_protein 9.955
IPC_protein 10.862
Toseland 10.891
ProMoST 10.745
Dawson 10.979
Bjellqvist 10.73
Wikipedia 11.228
Rodwell 11.155
Grimsley 11.038
Solomon 11.14
Lehninger 11.096
Nozaki 10.877
DTASelect 10.73
Thurlkill 10.891
EMBOSS 11.301
Sillero 10.921
Patrickios 10.906
IPC_peptide 11.155
IPC2_peptide 9.794
IPC2.peptide.svr19 8.347
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
533
0
533
128820
50
1581
241.7
27.49
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.481 ± 0.107
2.25 ± 0.094
4.923 ± 0.077
7.46 ± 0.119
4.565 ± 0.069
6.052 ± 0.212
1.863 ± 0.053
5.54 ± 0.073
5.995 ± 0.15
11.025 ± 0.155
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.903 ± 0.049
4.46 ± 0.08
4.342 ± 0.09
3.641 ± 0.067
5.245 ± 0.097
7.434 ± 0.113
5.333 ± 0.162
6.522 ± 0.078
1.306 ± 0.069
4.648 ± 0.077
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here