Halhan virus 2
Average proteome isoelectric point is 5.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3G2LQK0|A0A3G2LQK0_9VIRU Protein 2 OS=Halhan virus 2 OX=2480177 PE=4 SV=1
MM1 pKa = 7.03 YY2 pKa = 10.33 LWLLFAVAAGQQVMLDD18 pKa = 3.58 YY19 pKa = 10.62 QQYY22 pKa = 9.65 EE23 pKa = 4.22 GLIEE27 pKa = 5.57 AITSSHH33 pKa = 6.45 ASSIKK38 pKa = 8.8 TDD40 pKa = 3.46 TVIPEE45 pKa = 4.18 SEE47 pKa = 3.96 VKK49 pKa = 10.5 AFHH52 pKa = 6.4 FHH54 pKa = 6.08 FLKK57 pKa = 10.98 LGTFVSNVSIDD68 pKa = 3.41 LTLDD72 pKa = 2.89 VGYY75 pKa = 9.79 YY76 pKa = 9.86 IPITQFYY83 pKa = 8.58 GCNVSFFPHH92 pKa = 6.73 GAITTQNRR100 pKa = 11.84 SAQDD104 pKa = 3.34 FKK106 pKa = 11.87 ALVQEE111 pKa = 4.5 MMDD114 pKa = 3.63 FHH116 pKa = 7.97 QMLVRR121 pKa = 11.84 FTNRR125 pKa = 11.84 VWILQNVGGKK135 pKa = 9.78 LGSGSLHH142 pKa = 6.26 VPLLMINGLGLRR154 pKa = 11.84 FINTNIPFFEE164 pKa = 4.61 KK165 pKa = 10.39 PLLLSEE171 pKa = 4.28 ITDD174 pKa = 3.55 AYY176 pKa = 10.62 YY177 pKa = 10.12 IFYY180 pKa = 10.94 EE181 pKa = 4.44 HH182 pKa = 6.69 MFKK185 pKa = 9.83 TAQRR189 pKa = 11.84 AGSTGNFLNIYY200 pKa = 10.46 DD201 pKa = 3.84 MSLYY205 pKa = 10.7 SSIPITGVSTSSVGFGICKK224 pKa = 9.94 NDD226 pKa = 3.34 NKK228 pKa = 10.41 VGTQVPVCVNVAYY241 pKa = 9.54 TEE243 pKa = 3.78 QDD245 pKa = 3.37 YY246 pKa = 9.37 TITSLGRR253 pKa = 11.84 KK254 pKa = 9.28 VYY256 pKa = 7.99 MTIHH260 pKa = 6.13 GMDD263 pKa = 3.2 KK264 pKa = 10.26 FYY266 pKa = 10.94 HH267 pKa = 5.22 QFEE270 pKa = 4.08 ITLNFTALFTTAQKK284 pKa = 10.62 EE285 pKa = 3.97 DD286 pKa = 4.13 SEE288 pKa = 5.19 IKK290 pKa = 10.26 LQPAPIIQRR299 pKa = 11.84 TPMSFNYY306 pKa = 9.73 QYY308 pKa = 10.04 WHH310 pKa = 7.25 AAPGIYY316 pKa = 9.51 PVAIFFHH323 pKa = 7.5 DD324 pKa = 4.13 EE325 pKa = 3.99 FSFIPPPGYY334 pKa = 10.24 ASSYY338 pKa = 11.08 RR339 pKa = 11.84 LFQTDD344 pKa = 3.35 QGADD348 pKa = 3.27 SCAKK352 pKa = 10.11 YY353 pKa = 10.63 YY354 pKa = 10.69 VDD356 pKa = 4.02 PVEE359 pKa = 4.53 GALFLSQQPAPNCEE373 pKa = 3.91 FTVDD377 pKa = 5.49 SLFCLQGNNFVVTGAAAPAALGNFEE402 pKa = 4.8 RR403 pKa = 11.84 DD404 pKa = 3.18 LVVPRR409 pKa = 11.84 INYY412 pKa = 7.61 TVVNWYY418 pKa = 10.35 DD419 pKa = 3.51 FTLLGMPYY427 pKa = 8.2 YY428 pKa = 10.26 TPLGSSAYY436 pKa = 7.8 TSSYY440 pKa = 10.4 TIYY443 pKa = 10.25 QANPTSRR450 pKa = 11.84 LGSTAFDD457 pKa = 4.13 DD458 pKa = 4.39 ALDD461 pKa = 4.39 VISALPLPDD470 pKa = 3.89 GLRR473 pKa = 11.84 SIQDD477 pKa = 3.59 TIDD480 pKa = 3.7 DD481 pKa = 3.77 FRR483 pKa = 11.84 EE484 pKa = 4.34 FYY486 pKa = 11.08 DD487 pKa = 4.16 GVKK490 pKa = 10.68 DD491 pKa = 4.57 FWNTLQKK498 pKa = 9.76 IDD500 pKa = 4.84 EE501 pKa = 4.5 ILDD504 pKa = 3.55 VVKK507 pKa = 10.15 TFGAIFAA514 pKa = 5.07
Molecular weight: 57.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.756
IPC2_protein 4.825
IPC_protein 4.8
Toseland 4.698
ProMoST 4.952
Dawson 4.8
Bjellqvist 4.94
Wikipedia 4.711
Rodwell 4.685
Grimsley 4.622
Solomon 4.8
Lehninger 4.762
Nozaki 4.927
DTASelect 5.13
Thurlkill 4.711
EMBOSS 4.736
Sillero 4.978
Patrickios 0.922
IPC_peptide 4.8
IPC2_peptide 4.965
IPC2.peptide.svr19 4.829
Protein with the highest isoelectric point:
>tr|A0A3G2LQJ8|A0A3G2LQJ8_9VIRU Protein 3 OS=Halhan virus 2 OX=2480177 PE=4 SV=1
MM1 pKa = 7.88 RR2 pKa = 11.84 AFLDD6 pKa = 3.58 AMPDD10 pKa = 2.93 EE11 pKa = 5.44 FYY13 pKa = 10.78 RR14 pKa = 11.84 PKK16 pKa = 10.38 IPTAPPPPPDD26 pKa = 4.09 LNAAPKK32 pKa = 10.3 SSAASTMTIDD42 pKa = 3.89 ASPVVLRR49 pKa = 11.84 PVQAKK54 pKa = 9.63 PLLKK58 pKa = 10.49 KK59 pKa = 10.68 EE60 pKa = 4.53 GITNRR65 pKa = 11.84 STQDD69 pKa = 3.05 LSKK72 pKa = 11.35 NMPLPPLIRR81 pKa = 11.84 SSSNGDD87 pKa = 3.46 VVVGKK92 pKa = 10.74 IMDD95 pKa = 4.39 FKK97 pKa = 10.75 TFKK100 pKa = 10.7 AQQKK104 pKa = 8.56 PCKK107 pKa = 8.83 TPQPVVDD114 pKa = 4.04 ILQTKK119 pKa = 7.65 AVKK122 pKa = 10.08 YY123 pKa = 10.07 ASCDD127 pKa = 2.88 TDD129 pKa = 4.13 YY130 pKa = 11.62 NISACNRR137 pKa = 11.84 RR138 pKa = 11.84 YY139 pKa = 10.16 HH140 pKa = 6.23 SFVHH144 pKa = 6.53 ASYY147 pKa = 11.31 AVFDD151 pKa = 3.74 EE152 pKa = 4.58 VVNNRR157 pKa = 11.84 AIFEE161 pKa = 4.25 QVTAQEE167 pKa = 4.19 VSKK170 pKa = 11.07 QFALDD175 pKa = 3.41 MAQIFCADD183 pKa = 3.24 KK184 pKa = 10.36 TSYY187 pKa = 11.01 DD188 pKa = 3.08 IARR191 pKa = 11.84 CVVAALEE198 pKa = 4.35 DD199 pKa = 3.69 EE200 pKa = 4.16 EE201 pKa = 6.01 RR202 pKa = 11.84 EE203 pKa = 4.04 YY204 pKa = 10.79 FYY206 pKa = 11.46 EE207 pKa = 4.19 NLRR210 pKa = 11.84 DD211 pKa = 3.82 CSKK214 pKa = 9.91 TVADD218 pKa = 3.75 TTIADD223 pKa = 3.89 EE224 pKa = 4.51 LLDD227 pKa = 4.57 EE228 pKa = 4.34 IVYY231 pKa = 10.71 CIEE234 pKa = 3.75 QHH236 pKa = 5.67 NKK238 pKa = 9.92 KK239 pKa = 10.23 NDD241 pKa = 4.03 AIQRR245 pKa = 11.84 QALPEE250 pKa = 3.94 YY251 pKa = 10.87 LLLKK255 pKa = 10.3 PSAFLKK261 pKa = 10.61 PYY263 pKa = 7.4 MTGNSFKK270 pKa = 11.25 GKK272 pKa = 9.79 DD273 pKa = 3.4 AEE275 pKa = 4.46 EE276 pKa = 4.35 ACTTGFGQYY285 pKa = 9.96 PNSNKK290 pKa = 9.34 FFYY293 pKa = 10.52 DD294 pKa = 3.13 KK295 pKa = 11.0 NFPYY299 pKa = 10.35 PKK301 pKa = 9.46 ICSKK305 pKa = 11.27 GDD307 pKa = 3.31 ANYY310 pKa = 7.5 YY311 pKa = 9.61 TPFHH315 pKa = 6.71 PLYY318 pKa = 10.36 VGTQMPPEE326 pKa = 4.59 PIIAFSDD333 pKa = 3.41 LTNYY337 pKa = 9.34 LAHH340 pKa = 6.97 HH341 pKa = 6.58 GADD344 pKa = 3.51 KK345 pKa = 10.56 IKK347 pKa = 10.47 KK348 pKa = 7.67 VTVALDD354 pKa = 3.38 VEE356 pKa = 4.79 IPNGLQRR363 pKa = 11.84 VPYY366 pKa = 8.78 YY367 pKa = 10.97 GVVLDD372 pKa = 4.17 VSDD375 pKa = 3.97 NTVHH379 pKa = 7.38 GIPFEE384 pKa = 4.1 VEE386 pKa = 3.6 PMKK389 pKa = 10.8 FSIVVTEE396 pKa = 3.91 PAVFEE401 pKa = 4.11 AAKK404 pKa = 9.12 MHH406 pKa = 7.21 AISQLAGEE414 pKa = 4.77 KK415 pKa = 9.65 SQIYY419 pKa = 9.81 KK420 pKa = 10.08 EE421 pKa = 4.23 LHH423 pKa = 5.83 NQTADD428 pKa = 3.36 YY429 pKa = 10.71 LRR431 pKa = 11.84 NLEE434 pKa = 4.14 PQVSIINVMNGTYY447 pKa = 10.78 GCGKK451 pKa = 6.71 TTAIKK456 pKa = 10.89 QMMKK460 pKa = 10.42 KK461 pKa = 10.06 EE462 pKa = 4.25 GQNHH466 pKa = 6.15 EE467 pKa = 4.33 NTVVCCPSRR476 pKa = 11.84 PLANDD481 pKa = 3.43 YY482 pKa = 11.51 GKK484 pKa = 10.88 GAYY487 pKa = 7.36 TWARR491 pKa = 11.84 AFCRR495 pKa = 11.84 AMEE498 pKa = 4.3 LLEE501 pKa = 4.15 MNNLNAIYY509 pKa = 9.79 IDD511 pKa = 3.77 EE512 pKa = 4.61 VYY514 pKa = 9.07 NTHH517 pKa = 6.79 PLIVLALGSLGVPTYY532 pKa = 10.89 AIGDD536 pKa = 3.93 PRR538 pKa = 11.84 QMRR541 pKa = 11.84 PGGEE545 pKa = 3.81 GANYY549 pKa = 9.79 KK550 pKa = 10.18 IKK552 pKa = 9.73 TLDD555 pKa = 3.57 EE556 pKa = 4.02 LWAGKK561 pKa = 8.17 VTDD564 pKa = 3.76 YY565 pKa = 11.34 RR566 pKa = 11.84 DD567 pKa = 3.36 VSFSAPIDD575 pKa = 3.66 VVNTFNKK582 pKa = 9.19 EE583 pKa = 4.06 YY584 pKa = 10.42 EE585 pKa = 4.25 RR586 pKa = 11.84 KK587 pKa = 8.01 TYY589 pKa = 9.33 TLSTVFEE596 pKa = 4.45 SVVYY600 pKa = 10.32 KK601 pKa = 10.48 SIKK604 pKa = 9.18 SVKK607 pKa = 9.53 EE608 pKa = 3.82 IPAHH612 pKa = 6.15 CDD614 pKa = 3.11 KK615 pKa = 11.1 NCKK618 pKa = 8.9 EE619 pKa = 4.08 DD620 pKa = 3.74 HH621 pKa = 5.93 EE622 pKa = 5.27 HH623 pKa = 6.93 NLLLVFTTRR632 pKa = 11.84 AQDD635 pKa = 3.47 TTKK638 pKa = 9.95 GTTVASAQGLRR649 pKa = 11.84 KK650 pKa = 9.77 KK651 pKa = 10.63 SIDD654 pKa = 3.28 MFFEE658 pKa = 3.99 QNAAGCFRR666 pKa = 11.84 IHH668 pKa = 5.65 GQKK671 pKa = 10.47 LVGFTRR677 pKa = 11.84 HH678 pKa = 4.67 TEE680 pKa = 3.69 KK681 pKa = 11.23 LNVYY685 pKa = 8.34 TCAKK689 pKa = 5.86 TTLANKK695 pKa = 9.49 IRR697 pKa = 11.84 FTAPTIADD705 pKa = 3.63 RR706 pKa = 11.84 VGARR710 pKa = 11.84 HH711 pKa = 7.02 DD712 pKa = 3.32 FATYY716 pKa = 9.96 HH717 pKa = 6.61 GEE719 pKa = 3.94 FDD721 pKa = 4.63 LLSASYY727 pKa = 10.83 IDD729 pKa = 4.11 GEE731 pKa = 4.93 KK732 pKa = 10.04 IKK734 pKa = 11.15 CVDD737 pKa = 3.3 KK738 pKa = 10.7 VGKK741 pKa = 10.27 VITPSRR747 pKa = 11.84 PEE749 pKa = 3.39 RR750 pKa = 11.84 VEE752 pKa = 4.39 RR753 pKa = 11.84 IEE755 pKa = 4.09 EE756 pKa = 3.86 LAAEE760 pKa = 4.3 NQGKK764 pKa = 9.5 AVFTLPSEE772 pKa = 4.43 YY773 pKa = 10.62 LATHH777 pKa = 6.28 YY778 pKa = 11.24 AHH780 pKa = 6.52 VRR782 pKa = 11.84 PPVDD786 pKa = 3.53 SEE788 pKa = 4.29 LPQLATLEE796 pKa = 4.21 EE797 pKa = 4.12 QDD799 pKa = 4.64 VLVQEE804 pKa = 4.52 QPGTTTFGTNDD815 pKa = 3.52 DD816 pKa = 4.06 PQYY819 pKa = 9.43 TAQVLTQIAAPALGPIPSQRR839 pKa = 11.84 EE840 pKa = 3.79 VNILKK845 pKa = 9.99 IAAMKK850 pKa = 10.6 KK851 pKa = 7.26 NTTMHH856 pKa = 5.15 VHH858 pKa = 5.56 TDD860 pKa = 3.25 KK861 pKa = 11.25 PLVTQCEE868 pKa = 4.53 KK869 pKa = 9.4 PTHH872 pKa = 6.36 AFMAPIFGVKK882 pKa = 7.48 QTGQSTHH889 pKa = 6.12 VINTTVEE896 pKa = 3.92 RR897 pKa = 11.84 YY898 pKa = 9.44 AAVSSVPIKK907 pKa = 10.72 KK908 pKa = 9.7 RR909 pKa = 11.84 DD910 pKa = 3.35 VEE912 pKa = 4.58 SYY914 pKa = 10.54 SEE916 pKa = 4.16 KK917 pKa = 10.87 LLTGLAKK924 pKa = 10.43 FVDD927 pKa = 3.9 TDD929 pKa = 3.36 KK930 pKa = 10.99 FRR932 pKa = 11.84 PPTTAEE938 pKa = 3.97 FEE940 pKa = 4.33 ACRR943 pKa = 11.84 SQTLLRR949 pKa = 11.84 VAAKK953 pKa = 9.7 KK954 pKa = 10.11 QYY956 pKa = 9.58 PEE958 pKa = 4.01 PEE960 pKa = 3.98 IYY962 pKa = 10.51 GQTVQNTMKK971 pKa = 10.29 IKK973 pKa = 10.81 SFTKK977 pKa = 9.86 KK978 pKa = 9.13 QLKK981 pKa = 10.32 SKK983 pKa = 10.92 VGDD986 pKa = 4.07 FQDD989 pKa = 5.53 CMIKK993 pKa = 9.69 DD994 pKa = 3.4 TGSDD998 pKa = 3.64 YY999 pKa = 10.88 KK1000 pKa = 11.49 VKK1002 pKa = 10.36 GGQMVCAQTKK1012 pKa = 8.91 QCNEE1016 pKa = 3.46 STAAYY1021 pKa = 8.72 VAWAEE1026 pKa = 4.07 RR1027 pKa = 11.84 QVMEE1031 pKa = 4.24 NLRR1034 pKa = 11.84 EE1035 pKa = 4.06 GVMLGYY1041 pKa = 10.82 GDD1043 pKa = 3.51 STSRR1047 pKa = 11.84 LRR1049 pKa = 11.84 TRR1051 pKa = 11.84 ISRR1054 pKa = 11.84 RR1055 pKa = 11.84 IRR1057 pKa = 11.84 KK1058 pKa = 8.46 CRR1060 pKa = 11.84 KK1061 pKa = 4.39 TTYY1064 pKa = 10.18 EE1065 pKa = 4.31 CVSCDD1070 pKa = 3.44 MDD1072 pKa = 4.5 QQDD1075 pKa = 3.68 TTKK1078 pKa = 10.93 NDD1080 pKa = 3.26 AVNLYY1085 pKa = 8.05 MRR1087 pKa = 11.84 KK1088 pKa = 9.29 IYY1090 pKa = 10.13 RR1091 pKa = 11.84 SCGVPDD1097 pKa = 4.96 KK1098 pKa = 10.78 IIDD1101 pKa = 3.72 MLEE1104 pKa = 3.93 MPNEE1108 pKa = 3.74 KK1109 pKa = 9.46 WLMKK1113 pKa = 10.29 TKK1115 pKa = 8.02 TTSVNVNKK1123 pKa = 9.84 QFQSGRR1129 pKa = 11.84 SDD1131 pKa = 3.25 TLFANTMHH1139 pKa = 7.19 SIGEE1143 pKa = 4.05 AGKK1146 pKa = 10.13 SFEE1149 pKa = 4.36 IRR1151 pKa = 11.84 EE1152 pKa = 4.04 LMLALFQGDD1161 pKa = 3.67 DD1162 pKa = 3.63 SYY1164 pKa = 11.98 IRR1166 pKa = 11.84 AHH1168 pKa = 7.1 KK1169 pKa = 9.41 IAKK1172 pKa = 9.13 TSDD1175 pKa = 2.88 FFEE1178 pKa = 4.21 RR1179 pKa = 11.84 LKK1181 pKa = 10.83 VDD1183 pKa = 3.55 RR1184 pKa = 11.84 NLVGDD1189 pKa = 3.87 FVGLVIGTDD1198 pKa = 3.55 DD1199 pKa = 3.67 VSIDD1203 pKa = 3.16 IPRR1206 pKa = 11.84 ITGKK1210 pKa = 10.56 LLTRR1214 pKa = 11.84 SMIDD1218 pKa = 2.96 NARR1221 pKa = 11.84 YY1222 pKa = 10.05 EE1223 pKa = 4.43 EE1224 pKa = 4.2 IKK1226 pKa = 10.71 LAARR1230 pKa = 11.84 DD1231 pKa = 4.1 MIKK1234 pKa = 8.79 ITCTPEE1240 pKa = 3.8 TTRR1243 pKa = 11.84 KK1244 pKa = 10.07 AILYY1248 pKa = 9.14 NAFKK1252 pKa = 8.17 YY1253 pKa = 10.36 HH1254 pKa = 6.2 CMEE1257 pKa = 4.06 GTMSSLLEE1265 pKa = 3.93 FLQFFADD1272 pKa = 4.02 PRR1274 pKa = 11.84 RR1275 pKa = 11.84 GTPPEE1280 pKa = 4.27 KK1281 pKa = 9.72 ATKK1284 pKa = 10.61 DD1285 pKa = 3.26 NATLLKK1291 pKa = 10.4 IIITPFMVSS1300 pKa = 2.79
Molecular weight: 145.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.013
IPC2_protein 7.454
IPC_protein 7.234
Toseland 6.927
ProMoST 7.849
Dawson 7.966
Bjellqvist 8.375
Wikipedia 7.878
Rodwell 8.009
Grimsley 7.0
Solomon 7.995
Lehninger 8.009
Nozaki 8.58
DTASelect 8.097
Thurlkill 8.156
EMBOSS 8.185
Sillero 8.478
Patrickios 4.266
IPC_peptide 7.98
IPC2_peptide 7.293
IPC2.peptide.svr19 7.443
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
2479
306
1300
619.8
69.28
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.906 ± 0.357
1.493 ± 0.558
6.091 ± 0.201
4.962 ± 0.728
5.365 ± 0.904
5.607 ± 0.596
2.259 ± 0.26
5.728 ± 0.495
6.051 ± 1.35
7.543 ± 0.538
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.259 ± 0.474
4.155 ± 0.261
5.325 ± 0.262
4.316 ± 0.342
4.316 ± 0.473
6.172 ± 0.634
8.31 ± 0.399
7.261 ± 0.435
0.565 ± 0.202
4.316 ± 0.399
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here