Bos taurus papillomavirus 20
Average proteome isoelectric point is 6.54
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1B2K242|A0A1B2K242_9PAPI E4 protein OS=Bos taurus papillomavirus 20 OX=1887218 GN=E4 PE=4 SV=1
MM1 pKa = 7.24 VRR3 pKa = 11.84 AQRR6 pKa = 11.84 RR7 pKa = 11.84 KK8 pKa = 9.5 RR9 pKa = 11.84 ASEE12 pKa = 3.67 DD13 pKa = 2.98 TLYY16 pKa = 10.56 RR17 pKa = 11.84 GCLAGQDD24 pKa = 4.1 CPPDD28 pKa = 3.18 IKK30 pKa = 10.94 AKK32 pKa = 10.74 YY33 pKa = 8.54 EE34 pKa = 3.8 QDD36 pKa = 3.29 TLADD40 pKa = 5.53 RR41 pKa = 11.84 ILKK44 pKa = 8.66 WVSTFLYY51 pKa = 9.77 FGHH54 pKa = 7.29 LGISSGKK61 pKa = 8.13 GTGGQGGYY69 pKa = 8.03 TRR71 pKa = 11.84 LGGSGVSGGKK81 pKa = 7.96 GTNVTRR87 pKa = 11.84 PTVLVDD93 pKa = 3.71 ALPPPGVPIDD103 pKa = 3.93 TVSPDD108 pKa = 3.12 SSIIPLLTDD117 pKa = 2.93 SGGATTTDD125 pKa = 5.27 LIPGTGEE132 pKa = 3.57 IEE134 pKa = 4.2 IVAEE138 pKa = 3.91 VHH140 pKa = 6.31 PPPTNGQDD148 pKa = 3.31 SVIIGLEE155 pKa = 3.8 PEE157 pKa = 4.29 PPVLEE162 pKa = 4.21 VTPEE166 pKa = 3.85 HH167 pKa = 6.3 TPIAPRR173 pKa = 11.84 TRR175 pKa = 11.84 TTVSKK180 pKa = 10.74 HH181 pKa = 6.31 DD182 pKa = 3.66 NPAFNAYY189 pKa = 9.0 VASTQLPAEE198 pKa = 4.29 TAASDD203 pKa = 3.7 NVHH206 pKa = 6.43 IFHH209 pKa = 7.07 GFGGEE214 pKa = 3.99 IIGATEE220 pKa = 3.9 GATFEE225 pKa = 4.66 EE226 pKa = 5.01 IPLEE230 pKa = 4.2 EE231 pKa = 4.87 FNPPTQGPSSSTPEE245 pKa = 3.71 SGFRR249 pKa = 11.84 GVLDD253 pKa = 3.74 RR254 pKa = 11.84 FQRR257 pKa = 11.84 RR258 pKa = 11.84 LYY260 pKa = 10.47 NRR262 pKa = 11.84 RR263 pKa = 11.84 LVQQVKK269 pKa = 5.96 VTRR272 pKa = 11.84 RR273 pKa = 11.84 SFLDD277 pKa = 3.5 RR278 pKa = 11.84 PSSLVRR284 pKa = 11.84 WEE286 pKa = 4.35 FDD288 pKa = 3.06 NPTYY292 pKa = 10.95 EE293 pKa = 5.21 DD294 pKa = 4.16 DD295 pKa = 3.77 VSLLFEE301 pKa = 4.15 QDD303 pKa = 3.3 VHH305 pKa = 6.65 NVSAAPDD312 pKa = 3.85 LDD314 pKa = 3.79 FQDD317 pKa = 3.75 VVKK320 pKa = 10.78 LSRR323 pKa = 11.84 PHH325 pKa = 7.77 IYY327 pKa = 10.15 EE328 pKa = 3.66 KK329 pKa = 10.56 EE330 pKa = 4.15 GYY332 pKa = 9.3 IRR334 pKa = 11.84 LSRR337 pKa = 11.84 LGKK340 pKa = 9.98 KK341 pKa = 8.77 ATISTRR347 pKa = 11.84 SGTTIGSQVHH357 pKa = 5.95 YY358 pKa = 10.39 YY359 pKa = 10.89 SDD361 pKa = 4.34 LSPIQPVEE369 pKa = 4.44 EE370 pKa = 4.46 IPLHH374 pKa = 5.56 TFSSTDD380 pKa = 2.84 RR381 pKa = 11.84 SVIMQPLEE389 pKa = 3.63 EE390 pKa = 4.15 SVIIGSEE397 pKa = 4.11 YY398 pKa = 10.06 FDD400 pKa = 4.22 EE401 pKa = 4.54 NAVIYY406 pKa = 11.1 NEE408 pKa = 4.23 DD409 pKa = 3.51 TSLLPEE415 pKa = 4.4 FADD418 pKa = 5.21 DD419 pKa = 4.94 VLDD422 pKa = 4.96 DD423 pKa = 4.63 PYY425 pKa = 11.6 EE426 pKa = 4.09 EE427 pKa = 5.68 DD428 pKa = 3.6 FANTRR433 pKa = 11.84 LEE435 pKa = 4.1 VSGSSSRR442 pKa = 11.84 QTIYY446 pKa = 10.97 VPDD449 pKa = 5.59 GIPPGSVRR457 pKa = 11.84 AFVTDD462 pKa = 3.73 YY463 pKa = 11.05 NNHH466 pKa = 5.13 STVTPEE472 pKa = 4.33 GSNPLQPDD480 pKa = 4.07 IIHH483 pKa = 7.2 PDD485 pKa = 3.16 IVPDD489 pKa = 4.65 IILDD493 pKa = 3.75 IYY495 pKa = 10.35 TPGSTFYY502 pKa = 10.65 LHH504 pKa = 6.96 PSHH507 pKa = 6.28 YY508 pKa = 9.65 RR509 pKa = 11.84 RR510 pKa = 11.84 RR511 pKa = 11.84 GRR513 pKa = 11.84 KK514 pKa = 8.75 RR515 pKa = 11.84 KK516 pKa = 8.64 RR517 pKa = 11.84 TVFF520 pKa = 3.39
Molecular weight: 57.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.936
IPC2_protein 4.94
IPC_protein 4.914
Toseland 4.838
ProMoST 5.054
Dawson 4.914
Bjellqvist 5.041
Wikipedia 4.787
Rodwell 4.813
Grimsley 4.762
Solomon 4.902
Lehninger 4.863
Nozaki 5.029
DTASelect 5.207
Thurlkill 4.851
EMBOSS 4.825
Sillero 5.092
Patrickios 3.999
IPC_peptide 4.914
IPC2_peptide 5.092
IPC2.peptide.svr19 5.045
Protein with the highest isoelectric point:
>tr|A0A1B2K224|A0A1B2K224_9PAPI Replication protein E1 OS=Bos taurus papillomavirus 20 OX=1887218 GN=E1 PE=3 SV=1
MM1 pKa = 7.66 EE2 pKa = 4.54 EE3 pKa = 3.23 LAARR7 pKa = 11.84 FDD9 pKa = 4.08 AVQEE13 pKa = 3.96 RR14 pKa = 11.84 LMQIYY19 pKa = 10.16 EE20 pKa = 4.32 SGSVTLEE27 pKa = 3.75 SQIEE31 pKa = 4.34 YY32 pKa = 6.25 WTVVRR37 pKa = 11.84 KK38 pKa = 9.83 EE39 pKa = 3.55 YY40 pKa = 11.08 ALYY43 pKa = 8.85 YY44 pKa = 8.01 TARR47 pKa = 11.84 KK48 pKa = 9.46 QGIKK52 pKa = 10.45 KK53 pKa = 10.04 LGLYY57 pKa = 8.15 VVPSLQIAEE66 pKa = 4.36 QKK68 pKa = 10.58 AKK70 pKa = 10.36 EE71 pKa = 4.3 AIRR74 pKa = 11.84 MTLYY78 pKa = 10.94 LEE80 pKa = 4.52 SLSKK84 pKa = 10.96 SQFGDD89 pKa = 3.8 LQWTLPEE96 pKa = 4.14 TSLEE100 pKa = 4.3 TFSAPPANTFKK111 pKa = 11.1 KK112 pKa = 10.13 KK113 pKa = 9.63 GAQAIVTYY121 pKa = 10.98 DD122 pKa = 3.5 NDD124 pKa = 3.83 PEE126 pKa = 3.98 NRR128 pKa = 11.84 MMYY131 pKa = 8.24 TVWKK135 pKa = 9.19 EE136 pKa = 3.56 LYY138 pKa = 9.37 YY139 pKa = 10.47 QDD141 pKa = 5.65 DD142 pKa = 3.72 EE143 pKa = 4.81 EE144 pKa = 4.35 MWHH147 pKa = 6.65 KK148 pKa = 11.18 GEE150 pKa = 4.15 STVNHH155 pKa = 6.08 EE156 pKa = 4.34 GIYY159 pKa = 10.64 YY160 pKa = 10.07 VDD162 pKa = 3.14 NTGRR166 pKa = 11.84 TVYY169 pKa = 9.71 YY170 pKa = 10.59 QRR172 pKa = 11.84 FAQDD176 pKa = 3.15 AEE178 pKa = 4.38 RR179 pKa = 11.84 FSKK182 pKa = 10.28 SGQWNVQYY190 pKa = 10.72 EE191 pKa = 4.4 NQVFSAPVTSSSRR204 pKa = 11.84 QLRR207 pKa = 11.84 TPRR210 pKa = 11.84 GQGPEE215 pKa = 3.94 EE216 pKa = 4.24 RR217 pKa = 11.84 QDD219 pKa = 3.24 STGAQTHH226 pKa = 5.97 SPAAGPRR233 pKa = 11.84 QPAAPASRR241 pKa = 11.84 RR242 pKa = 11.84 PPVRR246 pKa = 11.84 PSLRR250 pKa = 11.84 GSRR253 pKa = 11.84 GSLSSAEE260 pKa = 3.88 RR261 pKa = 11.84 GPRR264 pKa = 11.84 RR265 pKa = 11.84 GRR267 pKa = 11.84 RR268 pKa = 11.84 GPRR271 pKa = 11.84 AGRR274 pKa = 11.84 QTSTSDD280 pKa = 2.84 GSSRR284 pKa = 11.84 SRR286 pKa = 11.84 SRR288 pKa = 11.84 SRR290 pKa = 11.84 SRR292 pKa = 11.84 TRR294 pKa = 11.84 SRR296 pKa = 11.84 SRR298 pKa = 11.84 SRR300 pKa = 11.84 GPEE303 pKa = 3.69 SGGQAPVSPEE313 pKa = 3.74 EE314 pKa = 3.86 VGTRR318 pKa = 11.84 TRR320 pKa = 11.84 TPEE323 pKa = 3.64 TRR325 pKa = 11.84 PTSRR329 pKa = 11.84 IAQLIEE335 pKa = 4.05 DD336 pKa = 4.11 ARR338 pKa = 11.84 DD339 pKa = 3.58 PPVLLLQGPANSLKK353 pKa = 10.35 CFRR356 pKa = 11.84 RR357 pKa = 11.84 RR358 pKa = 11.84 SKK360 pKa = 8.73 TQHH363 pKa = 6.53 PSLFLCMSTSWSWVCRR379 pKa = 11.84 TSTQKK384 pKa = 10.81 LGNRR388 pKa = 11.84 MLVAFSDD395 pKa = 3.9 ASQRR399 pKa = 11.84 TAFLSRR405 pKa = 11.84 VKK407 pKa = 10.53 LPRR410 pKa = 11.84 GVTWTTGSLNGLL422 pKa = 3.82
Molecular weight: 47.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.302
IPC2_protein 9.56
IPC_protein 10.218
Toseland 10.233
ProMoST 9.999
Dawson 10.438
Bjellqvist 10.16
Wikipedia 10.657
Rodwell 10.613
Grimsley 10.511
Solomon 10.482
Lehninger 10.438
Nozaki 10.218
DTASelect 10.16
Thurlkill 10.292
EMBOSS 10.643
Sillero 10.35
Patrickios 10.116
IPC_peptide 10.482
IPC2_peptide 9.048
IPC2.peptide.svr19 8.664
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2458
93
598
351.1
39.63
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.021 ± 0.647
2.034 ± 0.603
5.858 ± 0.669
6.347 ± 0.3
4.434 ± 0.706
6.387 ± 0.724
1.993 ± 0.217
4.231 ± 0.613
5.614 ± 0.973
8.747 ± 0.74
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.749 ± 0.358
3.662 ± 0.495
6.876 ± 0.916
5.004 ± 0.401
6.509 ± 1.066
7.608 ± 0.805
6.021 ± 0.915
6.021 ± 0.418
1.424 ± 0.284
3.458 ± 0.208
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here