Nova virus
Average proteome isoelectric point is 6.24
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0H3XTH4|A0A0H3XTH4_9VIRU Replicase OS=Nova virus OX=660955 PE=4 SV=1
MM1 pKa = 7.75 ADD3 pKa = 3.31 IEE5 pKa = 4.18 RR6 pKa = 11.84 LIAEE10 pKa = 4.16 GLEE13 pKa = 4.1 IEE15 pKa = 4.65 KK16 pKa = 10.84 EE17 pKa = 4.07 LMTARR22 pKa = 11.84 IRR24 pKa = 11.84 LQEE27 pKa = 4.12 AKK29 pKa = 9.95 EE30 pKa = 3.82 AAEE33 pKa = 4.14 RR34 pKa = 11.84 NPDD37 pKa = 3.48 EE38 pKa = 5.53 LNLKK42 pKa = 10.21 GVDD45 pKa = 3.24 ARR47 pKa = 11.84 QGVVTALEE55 pKa = 4.1 QKK57 pKa = 10.06 SAEE60 pKa = 4.11 NQRR63 pKa = 11.84 LIAEE67 pKa = 4.33 QRR69 pKa = 11.84 HH70 pKa = 5.13 TLPPTKK76 pKa = 9.58 PLKK79 pKa = 10.75 KK80 pKa = 9.42 PTDD83 pKa = 4.0 LDD85 pKa = 4.0 DD86 pKa = 6.12 DD87 pKa = 3.88 EE88 pKa = 6.31 HH89 pKa = 5.8 LTEE92 pKa = 5.41 RR93 pKa = 11.84 STLRR97 pKa = 11.84 YY98 pKa = 10.1 GSVIDD103 pKa = 6.07 LNDD106 pKa = 3.79 LDD108 pKa = 4.02 IDD110 pKa = 4.16 EE111 pKa = 5.35 PSGASANWVAICTYY125 pKa = 10.26 ILGFGATILLKK136 pKa = 10.5 ALYY139 pKa = 9.2 MLTTRR144 pKa = 11.84 GRR146 pKa = 11.84 QTIKK150 pKa = 8.54 EE151 pKa = 4.19 HH152 pKa = 5.76 KK153 pKa = 8.67 GNRR156 pKa = 11.84 VRR158 pKa = 11.84 FRR160 pKa = 11.84 DD161 pKa = 3.61 DD162 pKa = 2.7 SSYY165 pKa = 10.49 TEE167 pKa = 3.86 KK168 pKa = 11.18 GGVKK172 pKa = 9.74 VPKK175 pKa = 9.46 HH176 pKa = 6.18 LYY178 pKa = 9.93 VSLPTAQSAIKK189 pKa = 10.58 AEE191 pKa = 4.16 EE192 pKa = 3.92 LTPGRR197 pKa = 11.84 FRR199 pKa = 11.84 TIVCGLYY206 pKa = 9.89 PSEE209 pKa = 4.03 AKK211 pKa = 10.41 VRR213 pKa = 11.84 GLISPVMGVIGFQYY227 pKa = 10.69 LAQNWSEE234 pKa = 4.0 MIHH237 pKa = 7.3 RR238 pKa = 11.84 FMHH241 pKa = 5.41 MRR243 pKa = 11.84 CPFIEE248 pKa = 4.27 NPGLQVDD255 pKa = 4.22 SALLTNQAYY264 pKa = 10.3 FEE266 pKa = 4.24 MRR268 pKa = 11.84 SDD270 pKa = 3.51 ALEE273 pKa = 4.37 KK274 pKa = 10.51 IEE276 pKa = 4.01 AVEE279 pKa = 3.79 IRR281 pKa = 11.84 SIRR284 pKa = 11.84 EE285 pKa = 3.5 DD286 pKa = 4.74 AIDD289 pKa = 4.06 NDD291 pKa = 3.7 RR292 pKa = 11.84 KK293 pKa = 10.62 LINDD297 pKa = 4.22 ILQPDD302 pKa = 4.45 APWLFAGAPDD312 pKa = 4.01 RR313 pKa = 11.84 CPPTSLYY320 pKa = 10.52 VAGIPEE326 pKa = 4.96 LGAFLALMQDD336 pKa = 3.13 VRR338 pKa = 11.84 NAIMASKK345 pKa = 8.15 MTGTVEE351 pKa = 4.06 EE352 pKa = 4.48 KK353 pKa = 10.33 IKK355 pKa = 10.69 RR356 pKa = 11.84 KK357 pKa = 9.87 SSFYY361 pKa = 9.13 QSYY364 pKa = 8.58 MRR366 pKa = 11.84 RR367 pKa = 11.84 TQSMGMQMDD376 pKa = 3.31 QRR378 pKa = 11.84 IIHH381 pKa = 6.55 LFVMAWGKK389 pKa = 10.92 LMIDD393 pKa = 4.38 HH394 pKa = 7.05 FNLGDD399 pKa = 4.19 DD400 pKa = 3.91 MDD402 pKa = 4.77 PALRR406 pKa = 11.84 QMCQALLDD414 pKa = 4.09 EE415 pKa = 4.84 KK416 pKa = 11.38 VKK418 pKa = 10.31 EE419 pKa = 4.1 ICNNEE424 pKa = 3.9 PMRR427 pKa = 11.84 LL428 pKa = 3.6
Molecular weight: 48.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.665
IPC2_protein 5.652
IPC_protein 5.664
Toseland 5.931
ProMoST 5.944
Dawson 5.83
Bjellqvist 5.881
Wikipedia 5.804
Rodwell 5.804
Grimsley 6.02
Solomon 5.83
Lehninger 5.817
Nozaki 6.059
DTASelect 6.237
Thurlkill 6.186
EMBOSS 6.135
Sillero 6.148
Patrickios 4.431
IPC_peptide 5.842
IPC2_peptide 6.135
IPC2.peptide.svr19 6.212
Protein with the highest isoelectric point:
>tr|A0A0H3XVZ8|A0A0H3XVZ8_9VIRU Envelopment polyprotein OS=Nova virus OX=660955 PE=3 SV=1
MM1 pKa = 7.44 KK2 pKa = 10.2 RR3 pKa = 11.84 YY4 pKa = 9.96 LDD6 pKa = 3.64 LEE8 pKa = 4.15 RR9 pKa = 11.84 EE10 pKa = 4.16 IRR12 pKa = 11.84 QMPLSSLSATEE23 pKa = 4.76 CIGLLDD29 pKa = 3.13 QLYY32 pKa = 10.22 AVRR35 pKa = 11.84 HH36 pKa = 5.68 DD37 pKa = 5.18 LVDD40 pKa = 3.81 EE41 pKa = 4.55 MIKK44 pKa = 10.44 HH45 pKa = 6.42 DD46 pKa = 3.59 WSDD49 pKa = 3.56 NKK51 pKa = 10.95 DD52 pKa = 3.33 KK53 pKa = 8.92 EE54 pKa = 4.77 TPIVTVLMQAGIPDD68 pKa = 3.69 NSLGLIEE75 pKa = 4.98 KK76 pKa = 10.09 VVIPDD81 pKa = 3.52 HH82 pKa = 6.1 PTGKK86 pKa = 9.97 NLGAFMKK93 pKa = 7.62 TTPDD97 pKa = 2.97 NYY99 pKa = 10.44 KK100 pKa = 10.05 IEE102 pKa = 4.24 GTKK105 pKa = 8.67 ITFIEE110 pKa = 4.37 VTVTSDD116 pKa = 2.64 VDD118 pKa = 3.16 KK119 pKa = 11.19 GLKK122 pKa = 9.32 EE123 pKa = 4.18 KK124 pKa = 9.28 YY125 pKa = 9.29 QKK127 pKa = 10.67 YY128 pKa = 9.3 GSGMNTLQKK137 pKa = 10.63 EE138 pKa = 4.03 LDD140 pKa = 3.66 KK141 pKa = 10.99 LWRR144 pKa = 11.84 RR145 pKa = 11.84 GEE147 pKa = 3.98 LARR150 pKa = 11.84 QYY152 pKa = 10.58 TIIFNVVSVKK162 pKa = 10.71 NDD164 pKa = 2.86 GSNLSTQWPSRR175 pKa = 11.84 RR176 pKa = 11.84 NQGVVQHH183 pKa = 5.85 MRR185 pKa = 11.84 LLQALIQQVRR195 pKa = 11.84 EE196 pKa = 3.82 KK197 pKa = 10.77 LIKK200 pKa = 10.51 NEE202 pKa = 4.02 EE203 pKa = 4.11 KK204 pKa = 10.66 SVLEE208 pKa = 4.18 AMFNLKK214 pKa = 10.38 FNIGTQFVEE223 pKa = 4.44 TFNIPVFQGIQYY235 pKa = 9.15 EE236 pKa = 4.41 QIDD239 pKa = 4.43 CDD241 pKa = 4.09 ALLTYY246 pKa = 9.66 VKK248 pKa = 10.6 NWLSKK253 pKa = 10.38 DD254 pKa = 3.02 RR255 pKa = 11.84 KK256 pKa = 9.13 FAFNEE261 pKa = 3.9 VSGQAVKK268 pKa = 10.84 ASFKK272 pKa = 10.33 TYY274 pKa = 10.7 EE275 pKa = 4.16 DD276 pKa = 3.93 LQVASYY282 pKa = 9.79 KK283 pKa = 9.85 QSKK286 pKa = 9.34 KK287 pKa = 10.0 PRR289 pKa = 11.84 NFLLLQVAVQSDD301 pKa = 4.42 YY302 pKa = 11.57 KK303 pKa = 10.51 PATIVSDD310 pKa = 3.85 QLDD313 pKa = 3.68 TRR315 pKa = 11.84 YY316 pKa = 8.41 LTRR319 pKa = 11.84 VLHH322 pKa = 6.71 LEE324 pKa = 4.35 GADD327 pKa = 3.47 TPVQHH332 pKa = 6.85 LAKK335 pKa = 10.73 DD336 pKa = 3.37 MMMEE340 pKa = 4.86 FIQLEE345 pKa = 4.08 QVDD348 pKa = 3.74 IVSFYY353 pKa = 11.54 GNKK356 pKa = 9.2 QSFEE360 pKa = 4.19 RR361 pKa = 11.84 THH363 pKa = 6.35 KK364 pKa = 10.66 VPEE367 pKa = 4.68 PGTFKK372 pKa = 10.89 INMSKK377 pKa = 10.16 LHH379 pKa = 6.54 PDD381 pKa = 2.77 SRR383 pKa = 11.84 SFLEE387 pKa = 3.73 TLTRR391 pKa = 11.84 DD392 pKa = 3.1 KK393 pKa = 11.22 EE394 pKa = 4.0 KK395 pKa = 10.03 TKK397 pKa = 10.64 RR398 pKa = 11.84 VTGVYY403 pKa = 9.45 TPDD406 pKa = 3.79 EE407 pKa = 4.37 IEE409 pKa = 4.61 SISVKK414 pKa = 10.52 QNSEE418 pKa = 4.29 YY419 pKa = 10.63 NSCIAVIEE427 pKa = 4.67 SILTSLTTQLGNFEE441 pKa = 4.22 AEE443 pKa = 3.95 QRR445 pKa = 11.84 INPARR450 pKa = 11.84 TSIDD454 pKa = 3.18 RR455 pKa = 11.84 VLLKK459 pKa = 10.42 FMKK462 pKa = 10.75 NEE464 pKa = 3.35 ITKK467 pKa = 10.77 YY468 pKa = 9.48 MVDD471 pKa = 3.39 VLRR474 pKa = 11.84 KK475 pKa = 6.35 TVCWHH480 pKa = 5.88 VGHH483 pKa = 7.31 LTRR486 pKa = 11.84 DD487 pKa = 2.99 ITEE490 pKa = 3.94 ALIAHH495 pKa = 6.65 SGLKK499 pKa = 9.56 RR500 pKa = 11.84 SKK502 pKa = 9.22 YY503 pKa = 8.98 WSFHH507 pKa = 5.12 AFNNGNVGLFILPSKK522 pKa = 10.24 SLEE525 pKa = 3.98 VSGSYY530 pKa = 9.87 IRR532 pKa = 11.84 YY533 pKa = 6.43 FTVFKK538 pKa = 10.82 DD539 pKa = 3.27 GFGIVDD545 pKa = 4.14 HH546 pKa = 7.5 DD547 pKa = 4.86 NIDD550 pKa = 3.92 SIKK553 pKa = 10.34 MVDD556 pKa = 3.62 GVRR559 pKa = 11.84 WIYY562 pKa = 10.8 SKK564 pKa = 10.9 IMSVDD569 pKa = 3.83 LNRR572 pKa = 11.84 LMALNVAFEE581 pKa = 4.25 KK582 pKa = 11.07 ALVATAVWFQYY593 pKa = 10.24 YY594 pKa = 9.53 VEE596 pKa = 4.87 DD597 pKa = 3.7 QSHH600 pKa = 6.51 FPLITMVRR608 pKa = 11.84 NIFSIHH614 pKa = 5.83 YY615 pKa = 9.82 LLAITQKK622 pKa = 10.03 MKK624 pKa = 10.87 LCALFDD630 pKa = 4.37 NIRR633 pKa = 11.84 YY634 pKa = 8.45 LIPACTSQYY643 pKa = 11.07 SGYY646 pKa = 10.77 DD647 pKa = 3.18 SLIEE651 pKa = 4.03 KK652 pKa = 9.63 FVNRR656 pKa = 11.84 PFKK659 pKa = 10.67 SAIEE663 pKa = 3.92 IYY665 pKa = 10.67 VYY667 pKa = 11.04 DD668 pKa = 3.55 RR669 pKa = 11.84 AKK671 pKa = 10.73 RR672 pKa = 11.84 LLISLAQNNKK682 pKa = 7.77 FRR684 pKa = 11.84 YY685 pKa = 7.37 YY686 pKa = 11.11 SKK688 pKa = 11.24 VKK690 pKa = 10.63 LLGLCVDD697 pKa = 3.97 QSTVGASGIYY707 pKa = 9.81 PSLFGCAIFKK717 pKa = 10.2 HH718 pKa = 5.17 YY719 pKa = 10.58 RR720 pKa = 11.84 SVISEE725 pKa = 3.82 ATTCFFLFEE734 pKa = 4.85 KK735 pKa = 10.3 GLHH738 pKa = 4.79 GTMTEE743 pKa = 3.81 EE744 pKa = 4.3 AKK746 pKa = 10.3 IHH748 pKa = 6.36 LEE750 pKa = 3.99 TVEE753 pKa = 3.93 WANKK757 pKa = 9.48 IKK759 pKa = 10.55 EE760 pKa = 4.07 KK761 pKa = 8.89 EE762 pKa = 3.9 AKK764 pKa = 9.16 YY765 pKa = 10.56 GKK767 pKa = 10.05 EE768 pKa = 3.85 KK769 pKa = 11.15 VEE771 pKa = 4.23 NGYY774 pKa = 10.14 SLYY777 pKa = 10.84 EE778 pKa = 4.02 VMAGEE783 pKa = 4.34 VQVEE787 pKa = 3.95 QQLYY791 pKa = 9.45 CKK793 pKa = 10.51 DD794 pKa = 3.92 VVILAADD801 pKa = 3.93 EE802 pKa = 4.35 LNKK805 pKa = 10.05 VLIPKK810 pKa = 8.56 NQSVLSSIVNKK821 pKa = 10.26 HH822 pKa = 4.07 WDD824 pKa = 3.33 KK825 pKa = 11.16 PYY827 pKa = 10.31 FSQVRR832 pKa = 11.84 NISLKK837 pKa = 10.57 GMSGQLQEE845 pKa = 5.34 DD846 pKa = 3.95 GHH848 pKa = 7.24 LAASVTLIEE857 pKa = 4.79 AIRR860 pKa = 11.84 YY861 pKa = 7.99 LSSHH865 pKa = 6.19 SKK867 pKa = 10.5 NPSLMEE873 pKa = 4.39 LYY875 pKa = 10.85 NEE877 pKa = 4.24 TRR879 pKa = 11.84 HH880 pKa = 5.42 VRR882 pKa = 11.84 AQARR886 pKa = 11.84 IVRR889 pKa = 11.84 KK890 pKa = 8.7 HH891 pKa = 5.2 QRR893 pKa = 11.84 TEE895 pKa = 3.37 ADD897 pKa = 2.79 RR898 pKa = 11.84 GFFIVTMPTRR908 pKa = 11.84 VRR910 pKa = 11.84 LEE912 pKa = 4.25 IIEE915 pKa = 4.93 DD916 pKa = 4.15 YY917 pKa = 11.39 YY918 pKa = 11.64 DD919 pKa = 4.04 ALAKK923 pKa = 10.24 NVNEE927 pKa = 4.46 EE928 pKa = 4.25 YY929 pKa = 10.2 ISYY932 pKa = 10.57 GGEE935 pKa = 3.93 KK936 pKa = 10.1 KK937 pKa = 10.12 ILQIQTSLEE946 pKa = 3.94 RR947 pKa = 11.84 ALRR950 pKa = 11.84 WASGTSQLILSNDD963 pKa = 2.64 KK964 pKa = 10.87 RR965 pKa = 11.84 ILFKK969 pKa = 10.83 RR970 pKa = 11.84 KK971 pKa = 9.55 LMYY974 pKa = 10.59 VSADD978 pKa = 3.22 ATKK981 pKa = 9.98 WSPGDD986 pKa = 3.36 NSAKK990 pKa = 9.95 FRR992 pKa = 11.84 LFTAAVSNGMRR1003 pKa = 11.84 DD1004 pKa = 4.0 DD1005 pKa = 3.98 LLKK1008 pKa = 11.16 NCVIDD1013 pKa = 3.71 ALRR1016 pKa = 11.84 NIYY1019 pKa = 8.0 EE1020 pKa = 4.29 TEE1022 pKa = 3.97 FFLSRR1027 pKa = 11.84 RR1028 pKa = 11.84 LRR1030 pKa = 11.84 GYY1032 pKa = 10.1 LDD1034 pKa = 3.2 NMEE1037 pKa = 4.48 VKK1039 pKa = 10.49 SEE1041 pKa = 3.94 AVKK1044 pKa = 11.01 DD1045 pKa = 3.68 FLNFFDD1051 pKa = 4.38 FQKK1054 pKa = 10.52 EE1055 pKa = 3.66 RR1056 pKa = 11.84 SGLVRR1061 pKa = 11.84 GNWLQGNLNKK1071 pKa = 10.24 CSSLFAVGISFLFKK1085 pKa = 10.57 KK1086 pKa = 8.99 IWSMLFPEE1094 pKa = 4.99 LDD1096 pKa = 3.54 YY1097 pKa = 11.01 FIEE1100 pKa = 4.49 VAHH1103 pKa = 6.79 HH1104 pKa = 6.87 SDD1106 pKa = 3.56 DD1107 pKa = 3.6 ALFIYY1112 pKa = 9.84 GYY1114 pKa = 10.51 LEE1116 pKa = 4.35 PLDD1119 pKa = 5.7 DD1120 pKa = 4.72 GSDD1123 pKa = 2.52 WFMYY1127 pKa = 10.1 VSHH1130 pKa = 7.49 KK1131 pKa = 10.13 IQAGFYY1137 pKa = 7.81 YY1138 pKa = 9.51 WHH1140 pKa = 7.11 AVDD1143 pKa = 3.83 HH1144 pKa = 7.0 DD1145 pKa = 3.54 MWKK1148 pKa = 9.23 TMFNLHH1154 pKa = 5.28 EE1155 pKa = 4.92 HH1156 pKa = 6.58 LLLMGSIKK1164 pKa = 10.37 VSPKK1168 pKa = 8.96 KK1169 pKa = 7.75 TTVSPTNAEE1178 pKa = 3.94 FLSTFFEE1185 pKa = 4.95 GCAVSIPFTKK1195 pKa = 10.07 ILLGALSDD1203 pKa = 3.97 LPGLGFFDD1211 pKa = 5.32 DD1212 pKa = 4.49 LAAAQSRR1219 pKa = 11.84 CVKK1222 pKa = 10.44 ALDD1225 pKa = 3.65 MGANPQVAQLVVGLVNSKK1243 pKa = 8.44 VEE1245 pKa = 4.13 RR1246 pKa = 11.84 LYY1248 pKa = 10.18 GTAPGMVNNPTRR1260 pKa = 11.84 YY1261 pKa = 10.23 LNIPPEE1267 pKa = 5.03 DD1268 pKa = 3.56 IPICLGGTGPGSIMEE1283 pKa = 4.79 LATAGIAMADD1293 pKa = 3.32 KK1294 pKa = 10.85 CSLRR1298 pKa = 11.84 KK1299 pKa = 9.43 ALQTYY1304 pKa = 6.85 QHH1306 pKa = 6.88 KK1307 pKa = 10.41 ASKK1310 pKa = 10.2 SSSYY1314 pKa = 11.05 HH1315 pKa = 5.66 LGLFKK1320 pKa = 10.75 FLMLLSEE1327 pKa = 5.12 DD1328 pKa = 4.18 VFNHH1332 pKa = 5.82 DD1333 pKa = 4.1 TLGEE1337 pKa = 4.0 FCFTGKK1343 pKa = 9.28 VQWKK1347 pKa = 9.29 IFTPKK1352 pKa = 10.36 SEE1354 pKa = 4.48 FEE1356 pKa = 4.01 FHH1358 pKa = 7.24 DD1359 pKa = 5.05 LYY1361 pKa = 11.73 SPMIVKK1367 pKa = 9.82 NWTDD1371 pKa = 3.24 EE1372 pKa = 4.27 HH1373 pKa = 6.64 PSYY1376 pKa = 11.24 DD1377 pKa = 3.5 YY1378 pKa = 11.29 VVPTRR1383 pKa = 11.84 RR1384 pKa = 11.84 DD1385 pKa = 3.19 DD1386 pKa = 3.86 LLIYY1390 pKa = 10.01 LVRR1393 pKa = 11.84 RR1394 pKa = 11.84 LNEE1397 pKa = 3.9 PSIMAAMTLQSPIQLRR1413 pKa = 11.84 FRR1415 pKa = 11.84 MQAKK1419 pKa = 9.01 QHH1421 pKa = 6.36 LNVCRR1426 pKa = 11.84 YY1427 pKa = 8.43 KK1428 pKa = 10.82 SEE1430 pKa = 4.01 WVSFRR1435 pKa = 11.84 QLLAAADD1442 pKa = 3.95 VFAQEE1447 pKa = 4.68 YY1448 pKa = 10.44 NPSQNDD1454 pKa = 2.56 IDD1456 pKa = 5.57 LFQTLCNCTFSKK1468 pKa = 10.53 EE1469 pKa = 4.11 FAWRR1473 pKa = 11.84 DD1474 pKa = 3.34 FLNTVDD1480 pKa = 6.03 CEE1482 pKa = 4.25 VLPQKK1487 pKa = 10.4 RR1488 pKa = 11.84 IHH1490 pKa = 6.26 RR1491 pKa = 11.84 PKK1493 pKa = 9.34 VARR1496 pKa = 11.84 TFTVKK1501 pKa = 10.28 EE1502 pKa = 3.75 RR1503 pKa = 11.84 DD1504 pKa = 3.49 QAIQNPISVVIAYY1517 pKa = 9.26 RR1518 pKa = 11.84 FAKK1521 pKa = 10.09 KK1522 pKa = 10.01 PEE1524 pKa = 4.32 EE1525 pKa = 3.87 IRR1527 pKa = 11.84 DD1528 pKa = 3.57 VLKK1531 pKa = 10.72 NAKK1534 pKa = 9.97 FPDD1537 pKa = 4.56 SISNDD1542 pKa = 3.05 LDD1544 pKa = 3.64 VLHH1547 pKa = 6.85 KK1548 pKa = 10.53 GVHH1551 pKa = 6.07 RR1552 pKa = 11.84 EE1553 pKa = 3.91 LGLDD1557 pKa = 3.24 ISDD1560 pKa = 3.64 VAVMKK1565 pKa = 10.48 RR1566 pKa = 11.84 VAPMLYY1572 pKa = 9.1 KK1573 pKa = 10.4 SSRR1576 pKa = 11.84 SRR1578 pKa = 11.84 IVLVNGNVEE1587 pKa = 4.09 GTAEE1591 pKa = 4.47 SICSYY1596 pKa = 8.3 WLKK1599 pKa = 10.38 DD1600 pKa = 3.02 ISMMKK1605 pKa = 8.81 TIKK1608 pKa = 10.0 VRR1610 pKa = 11.84 PHH1612 pKa = 6.56 RR1613 pKa = 11.84 EE1614 pKa = 3.54 VLKK1617 pKa = 10.24 AVSIFGHH1624 pKa = 6.7 KK1625 pKa = 9.85 DD1626 pKa = 2.87 ASGDD1630 pKa = 3.91 KK1631 pKa = 10.27 KK1632 pKa = 10.9 DD1633 pKa = 3.7 LAALRR1638 pKa = 11.84 VCIEE1642 pKa = 3.47 VWRR1645 pKa = 11.84 WAKK1648 pKa = 10.95 HH1649 pKa = 4.58 NDD1651 pKa = 3.0 ADD1653 pKa = 3.77 VRR1655 pKa = 11.84 EE1656 pKa = 4.53 WFHH1659 pKa = 6.78 SLWFEE1664 pKa = 5.15 DD1665 pKa = 3.7 RR1666 pKa = 11.84 TFMDD1670 pKa = 3.95 WVNKK1674 pKa = 9.32 FQTKK1678 pKa = 9.8 GLPIVDD1684 pKa = 5.17 PEE1686 pKa = 4.17 IQCAGLMIADD1696 pKa = 4.67 LTRR1699 pKa = 11.84 DD1700 pKa = 3.48 EE1701 pKa = 6.06 SILQMQANRR1710 pKa = 11.84 RR1711 pKa = 11.84 AYY1713 pKa = 10.36 SGKK1716 pKa = 10.23 QYY1718 pKa = 11.01 DD1719 pKa = 3.99 AYY1721 pKa = 10.94 CIQTYY1726 pKa = 9.6 NEE1728 pKa = 3.89 EE1729 pKa = 4.08 KK1730 pKa = 10.36 KK1731 pKa = 10.65 QYY1733 pKa = 10.38 EE1734 pKa = 3.82 GDD1736 pKa = 3.47 LRR1738 pKa = 11.84 VTFNFGTDD1746 pKa = 3.45 CARR1749 pKa = 11.84 LEE1751 pKa = 4.26 IFWDD1755 pKa = 3.52 KK1756 pKa = 10.96 QDD1758 pKa = 3.95 YY1759 pKa = 10.87 LLEE1762 pKa = 4.04 TSITSRR1768 pKa = 11.84 HH1769 pKa = 4.76 VLKK1772 pKa = 10.89 IMMEE1776 pKa = 4.19 EE1777 pKa = 4.09 VSKK1780 pKa = 10.57 EE1781 pKa = 3.82 LQGCGMRR1788 pKa = 11.84 FKK1790 pKa = 10.52 TEE1792 pKa = 4.03 QRR1794 pKa = 11.84 SHH1796 pKa = 4.28 TTAVVLFKK1804 pKa = 10.61 TDD1806 pKa = 4.99 AGFEE1810 pKa = 4.02 WGKK1813 pKa = 10.01 PNVPCVVFTKK1823 pKa = 10.58 CVLRR1827 pKa = 11.84 TSLRR1831 pKa = 11.84 THH1833 pKa = 6.35 TAVKK1837 pKa = 9.77 HH1838 pKa = 6.03 DD1839 pKa = 3.77 FMIKK1843 pKa = 9.81 IVDD1846 pKa = 3.36 NGFRR1850 pKa = 11.84 AIAQYY1855 pKa = 11.33 DD1856 pKa = 3.77 LDD1858 pKa = 3.92 SPRR1861 pKa = 11.84 FMLAHH1866 pKa = 7.4 CYY1868 pKa = 10.19 HH1869 pKa = 6.79 ILKK1872 pKa = 10.18 DD1873 pKa = 3.59 VRR1875 pKa = 11.84 YY1876 pKa = 9.18 QAIDD1880 pKa = 3.41 TVGAIYY1886 pKa = 10.12 YY1887 pKa = 9.54 GRR1889 pKa = 11.84 RR1890 pKa = 11.84 NRR1892 pKa = 11.84 LYY1894 pKa = 10.58 LNPIISAGLFEE1905 pKa = 5.49 NFMKK1909 pKa = 10.88 GIPAVIPPTAYY1920 pKa = 10.81 SLIMNKK1926 pKa = 10.23 AKK1928 pKa = 10.49 ISVDD1932 pKa = 2.79 LFMFNKK1938 pKa = 9.2 LLAMINPNNVLNLEE1952 pKa = 4.31 GLHH1955 pKa = 6.09 PAEE1958 pKa = 4.51 TGYY1961 pKa = 8.72 STVTSISSTLWSEE1974 pKa = 3.99 EE1975 pKa = 3.96 MEE1977 pKa = 4.25 LEE1979 pKa = 4.11 EE1980 pKa = 6.47 AEE1982 pKa = 4.5 VEE1984 pKa = 3.93 DD1985 pKa = 4.57 DD1986 pKa = 4.58 EE1987 pKa = 5.42 YY1988 pKa = 11.85 VIDD1991 pKa = 5.47 LDD1993 pKa = 4.33 EE1994 pKa = 7.18 LDD1996 pKa = 4.56 FQDD1999 pKa = 4.72 ISYY2002 pKa = 10.78 QEE2004 pKa = 5.25 DD2005 pKa = 3.52 IEE2007 pKa = 5.71 HH2008 pKa = 6.33 YY2009 pKa = 9.7 LQEE2012 pKa = 3.95 EE2013 pKa = 4.46 TMYY2016 pKa = 11.24 GSDD2019 pKa = 4.78 LVIQSEE2025 pKa = 4.47 SLEE2028 pKa = 4.02 VSRR2031 pKa = 11.84 VRR2033 pKa = 11.84 GIVKK2037 pKa = 10.08 LNEE2040 pKa = 4.54 PIRR2043 pKa = 11.84 LIKK2046 pKa = 10.47 SWVSKK2051 pKa = 10.64 GLLVEE2056 pKa = 4.83 KK2057 pKa = 10.65 VFDD2060 pKa = 5.03 PIAIILITRR2069 pKa = 11.84 YY2070 pKa = 9.33 FSKK2073 pKa = 10.35 KK2074 pKa = 9.96 YY2075 pKa = 10.5 KK2076 pKa = 10.6 FGGQQVSTMDD2086 pKa = 4.19 PYY2088 pKa = 11.8 DD2089 pKa = 3.49 LTEE2092 pKa = 4.28 FEE2094 pKa = 4.32 AVVRR2098 pKa = 11.84 GWGEE2102 pKa = 3.81 LVFDD2106 pKa = 4.75 QFDD2109 pKa = 3.69 EE2110 pKa = 4.17 VDD2112 pKa = 3.73 RR2113 pKa = 11.84 QAKK2116 pKa = 9.4 EE2117 pKa = 3.64 YY2118 pKa = 10.01 AVKK2121 pKa = 10.67 YY2122 pKa = 9.93 NPSPEE2127 pKa = 4.03 EE2128 pKa = 4.53 LIPDD2132 pKa = 3.63 SVFSFRR2138 pKa = 11.84 HH2139 pKa = 5.18 TEE2141 pKa = 3.76 LLLNRR2146 pKa = 11.84 LFFKK2150 pKa = 10.9 DD2151 pKa = 3.67 KK2152 pKa = 10.1 LTSFYY2157 pKa = 11.62
Molecular weight: 248.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.536
IPC2_protein 6.707
IPC_protein 6.678
Toseland 6.766
ProMoST 7.205
Dawson 7.19
Bjellqvist 7.293
Wikipedia 7.161
Rodwell 7.19
Grimsley 6.898
Solomon 7.19
Lehninger 7.205
Nozaki 7.556
DTASelect 7.512
Thurlkill 7.556
EMBOSS 7.541
Sillero 7.629
Patrickios 4.291
IPC_peptide 7.205
IPC2_peptide 7.0
IPC2.peptide.svr19 6.892
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
3712
428
2157
1237.3
140.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.603 ± 0.738
2.613 ± 1.235
5.738 ± 0.367
5.819 ± 0.782
4.795 ± 0.453
5.603 ± 0.83
2.505 ± 0.187
6.573 ± 0.179
6.735 ± 0.44
9.537 ± 0.322
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.909 ± 0.334
4.122 ± 0.162
3.61 ± 0.32
4.095 ± 0.069
4.661 ± 0.799
7.166 ± 0.555
5.55 ± 0.521
7.004 ± 0.452
1.536 ± 0.152
3.825 ± 0.282
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here